Status
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Gene information
General information
TC034492
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001853,TC001855
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451507.1: 4165..10451
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC034492
      iBeetle screen iB_06684

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Adult morphology
      abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 4 (13 dpi)
      Days post injection


      abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 4 (13 dpi)
      Days post injection


      abdominal odoriferous glands content darker - Number of animals showing the phenotype: 4 (13 dpi)
      Days post injection



      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC034492 RA
        MKLYNKQTC DSQHSIDST LTKREDVIK CSQLSVKEK 
        QLANHAVAN IVQIVDEDD DFMVTLSSQ PDSELSFSL 
        PFTQYLAQH GIENDYSDD ISLTLIDQF INEDEAASN 
        PVVDEGTCP INNSVTDEL NVFDNDMVV VEEPPISPS 
        PPQSPFVEP VFTRPVTPT SPEAAPAVL TQPPMIISQ 
        DIIDALSEP WVEVENIIN GNVEHPPQP NSPIIEEND 
        VLPERLVVV EAENNIIRS PQPSPPPSP IILSQGVND 
        MQQPSLIDA LAEPWVEER RQGEEMDVE NDIIQPSQL 
        SPILGQINN RIENRVEGG DDDDDEDII PPTPPHSRS 
        RRRRRPRKP RRRSRKSPL ILIINNYNI NININDVNI 
        NN
      • >TC034492 RB
        MLEETMRHN KAMEMSRGK DSSLSQYLR EDCLEEDVI 
        KCSQLSVKE KQLANHAVA NIVQIVDED DDFMVTLSS 
        QPDSELSFS LPFTQYLAQ HGIENDYSD DISLTLIDQ 
        FINEDEAAS NPVVDEGTC PINNSVTDE LNVFDNDMV 
        VVEEPPISP SPPQSPFVE PVFTRPVTP TSPEAAPAV 
        LTQPPMIIS QDIIDALSE PWVEVENII NGNVEHPPQ 
        PNSPIIEEN DVLPERLVV VEAENNIIR SPQPSPPPS 
        PIILSQGVN DMQQPSLID ALAEPWVEE RRQGEEMDV 
        ENDIIQPSQ LSPILGQIN NRIENRVEG GDDDDDEDI 
        IPPTPPHSR SRRRRRPRK PRRRSRKSP LILIINNYN 
        INININDVN INN
      CDS sequence
      • >TC034492 RA
        atgaagtta tataataaa cagacttgt gattctcaa 
        catagtatt gattcaact ctgacaaag agagaggat 
        gtgattaaa tgtagtcag ttaagtgtg aaagagaag 
        caattagct aatcacgct gttgctaac attgttcaa 
        atcgttgat gaggatgat gattttatg gttactctt 
        tcatcacaa cctgatagt gaattatcg ttttcttta 
        ccatttacg caatacctg gctcaacat ggtattgaa 
        aatgattat tcggacgac atctcgttg accttaata 
        gaccagttt attaacgaa gacgaggcg gcgtccaat 
        ccggttgtg gatgaagga acttgtccc atcaataat 
        tcggttacc gatgagtta aatgtattt gataatgat 
        atggtggtg gtggaagaa cccccaatc tctcccagt 
        ccaccacaa agccccttc gttgagcca gtgtttacc 
        cgccccgtt acaccaaca tctcctgaa gcggcaccc 
        gcggtatta actcaaccc cctatgata atatcgcag 
        gacatcatt gacgcttta tcggagcct tgggttgag 
        gtggaaaac attatcaat ggtaacgtt gaacatccc 
        cctcaaccc aattcaccg attattgag gaaaatgat 
        gttctgcca gagagattg gtggtggta gaggccgaa 
        aataacatc attcggtca ccacaacca tcaccacca 
        ccgtcaccc ataatatta tcacagggc gtcaatgac 
        atgcaacaa ccatctctc attgacgca ctggcagag 
        ccgtgggtt gaagagagg aggcagggt gaagagatg 
        gatgttgaa aatgacatc attcaaccc tctcaattg 
        tcacccatt ctagggcaa ataaataat aggattgag 
        aatagggtg gagggtgga gacgatgac gacgacgaa 
        gacatcatt ccacccact ccccctcac tcacgatca 
        aggaggcgt cggcgccct agaaagcct cgtagacgt 
        tcgcgaaag tcgccatta atattaatt attaataat 
        tataatatt aatattaat attaatgat gttaatatt 
        aataattaa
      • >TC034492 RB
        atgttggag gaaactatg cggcataat aaagcaatg 
        gaaatgagt aggggtaaa gattcgtct ttaagtcaa 
        tatttgagg gaagattgt cttgaagag gatgtgatt 
        aaatgtagt cagttaagt gtgaaagag aagcaatta 
        gctaatcac gctgttgct aacattgtt caaatcgtt 
        gatgaggat gatgatttt atggttact ctttcatca 
        caacctgat agtgaatta tcgttttct ttaccattt 
        acgcaatac ctggctcaa catggtatt gaaaatgat 
        tattcggac gacatctcg ttgacctta atagaccag 
        tttattaac gaagacgag gcggcgtcc aatccggtt 
        gtggatgaa ggaacttgt cccatcaat aattcggtt 
        accgatgag ttaaatgta tttgataat gatatggtg 
        gtggtggaa gaaccccca atctctccc agtccacca 
        caaagcccc ttcgttgag ccagtgttt acccgcccc 
        gttacacca acatctcct gaagcggca cccgcggta 
        ttaactcaa ccccctatg ataatatcg caggacatc 
        attgacgct ttatcggag ccttgggtt gaggtggaa 
        aacattatc aatggtaac gttgaacat ccccctcaa 
        cccaattca ccgattatt gaggaaaat gatgttctg 
        ccagagaga ttggtggtg gtagaggcc gaaaataac 
        atcattcgg tcaccacaa ccatcacca ccaccgtca 
        cccataata ttatcacag ggcgtcaat gacatgcaa 
        caaccatct ctcattgac gcactggca gagccgtgg 
        gttgaagag aggaggcag ggtgaagag atggatgtt 
        gaaaatgac atcattcaa ccctctcaa ttgtcaccc 
        attctaggg caaataaat aataggatt gagaatagg 
        gtggagggt ggagacgat gacgacgac gaagacatc 
        attccaccc actccccct cactcacga tcaaggagg 
        cgtcggcgc cctagaaag cctcgtaga cgttcgcga 
        aagtcgcca ttaatatta attattaat aattataat 
        attaatatt aatattaat gatgttaat attaataat 
        taa
      mRNA sequence
      • >TC034492 RA
        tacttagaa aatagggtt tcgtttgtc ataagtgta 
        aaactccgt gttacacag ctagccaag tggatccgg 
        aaaatgccc aaaaaatta tagctatat accactaac 
        ttaaatcag cataatgga agttgacaa tgatcctta 
        ctgctacat gctcttttg cctatctcc aaagaactg 
        tagtaacag aaacttctt ttgtttgag atgtgtgaa 
        gcttatggc agtgagatt tcacatttg tcatctaca 
        tgtgacttc agtgttaaa ctacaagtg tttttggtg 
        taatacagt aataggaca gaattctca gttgtgtgg 
        aaataattt aataaacag aatgactgt taaaatgtg 
        gtattttag tagagagcg acaaatgat gttttatga 
        ttctgatga ctatgattc tgacgactc atcaagtag 
        ctgtgatag tggtataga atgttagtt aataagagt 
        agaaattag atataaaca acttgtaac cttagatta 
        aattagtat ctagattgt tcttttgct gtatggata 
        aaagtaaat aaatgaagt tatataata aacagactt 
        gtgattctc aacatagta ttgattcaa ctctgacaa 
        agagagagg atgtgatta aatgtagtc agttaagtg 
        tgaaagaga agcaattag ctaatcacg ctgttgcta 
        acattgttc aaatcgttg atgaggatg atgatttta 
        tggttactc tttcatcac aacctgata gtgaattat 
        cgttttctt taccattta cgcaatacc tggctcaac 
        atggtattg aaaatgatt attcggacg acatctcgt 
        tgaccttaa tagaccagt ttattaacg aagacgagg 
        cggcgtcca atccggttg tggatgaag gaacttgtc 
        ccatcaata attcggtta ccgatgagt taaatgtat 
        ttgataatg atatggtgg tggtggaag aacccccaa 
        tctctccca gtccaccac aaagcccct tcgttgagc 
        cagtgttta cccgccccg ttacaccaa catctcctg 
        aagcggcac ccgcggtat taactcaac cccctatga 
        taatatcgc aggacatca ttgacgctt tatcggagc 
        cttgggttg aggtggaaa acattatca atggtaacg 
        ttgaacatc cccctcaac ccaattcac cgattattg 
        aggaaaatg atgttctgc cagagagat tggtggtgg 
        tagaggccg aaaataaca tcattcggt caccacaac 
        catcaccac caccgtcac ccataatat tatcacagg 
        gcgtcaatg acatgcaac aaccatctc tcattgacg 
        cactggcag agccgtggg ttgaagaga ggaggcagg 
        gtgaagaga tggatgttg aaaatgaca tcattcaac 
        cctctcaat tgtcaccca ttctagggc aaataaata 
        ataggattg agaataggg tggagggtg gagacgatg 
        acgacgacg aagacatca ttccaccca ctccccctc 
        actcacgat caaggaggc gtcggcgcc ctagaaagc 
        ctcgtagac gttcgcgaa agtcgccat taatattaa 
        ttattaata attataata ttaatatta atattaatg 
        atgttaata ttaataatt aatattaat aaatcctat 
        ttccatcc
      • >TC034492 RB
        tacttagaa aatagggtt tcgtttgtc ataagtgta 
        aaactccgt gttacacag ctagccaag tggatccgg 
        aaaatgccc aaaaaatta tagctatat accactaac 
        ttaaatcag cataatgga agttgacaa tgatcctta 
        ctgctacat gctcttttg cctatctcc aaagaactg 
        tagtaacag aaacttctt ttgtttgag atgtgtgaa 
        gcttatggc agtgagatt tcacatttg tcatctaca 
        tgtgacttc agtgttaaa ctacaagtg tttttggtg 
        taatacagt aataggaca gaattctca gttgtgtgg 
        aaataattt aataaacag aatgactgt taaaatgtg 
        gtattttag tagagagcg acaaatgat gttttatga 
        ttctgatga ctatgattc tgacgactc atcaagtag 
        ctgtgatag tggtataga atgttagtt aataagagt 
        agaaattag atataaaca acttgtaac cttagatta 
        aattagtat ctagattgt tcttttgct gtatggata 
        aaagtaaat aaatgaagt tatataata aacagactt 
        gtgattctc aacatagta ttgattcaa ctctgacaa 
        agagaggtt ggggtctaa taaataaat taataaata 
        aattaccca ttgaattac atataccga gttattagt 
        tttgtggtc ctggaacca aattacaag aacgaattg 
        cacttggac atagtggta gaaataaat tagatcagg 
        cttgtaaag aacacgata tatcgtacg ccaacgaaa 
        ctaatttgc aaaaacgac acgaagcag ataagttat 
        tattaagta aagcagttg aacgtttaa aagcaaaag 
        acgcatcgt ttggcgaaa aagcagctg cattagcaa 
        tcgctggta taatgaaag gcaaagtaa aattcggaa 
        tgggagcta aaagtaaaa agggtaaaa aaaagaaga 
        caaaaaaac aacgagttc tagttgctc ctaaatatg 
        gtggttttt tactattcc tattaccac tattaggtg 
        ctctaggcg ctttaggag gaggagctg cagggatag 
        cgactgctg taaataaag catcggtag ataaaaaaa 
        tgttggagg aaactatgc ggcataata aagcaatgg 
        aaatgagta ggggtaaag attcgtctt taagtcaat 
        atttgaggg aagattgtc ttgaagagg atgtgatta 
        aatgtagtc agttaagtg tgaaagaga agcaattag 
        ctaatcacg ctgttgcta acattgttc aaatcgttg 
        atgaggatg atgatttta tggttactc tttcatcac 
        aacctgata gtgaattat cgttttctt taccattta 
        cgcaatacc tggctcaac atggtattg aaaatgatt 
        attcggacg acatctcgt tgaccttaa tagaccagt 
        ttattaacg aagacgagg cggcgtcca atccggttg 
        tggatgaag gaacttgtc ccatcaata attcggtta 
        ccgatgagt taaatgtat ttgataatg atatggtgg 
        tggtggaag aacccccaa tctctccca gtccaccac 
        aaagcccct tcgttgagc cagtgttta cccgccccg 
        ttacaccaa catctcctg aagcggcac ccgcggtat 
        taactcaac cccctatga taatatcgc aggacatca 
        ttgacgctt tatcggagc cttgggttg aggtggaaa 
        acattatca atggtaacg ttgaacatc cccctcaac 
        ccaattcac cgattattg aggaaaatg atgttctgc 
        cagagagat tggtggtgg tagaggccg aaaataaca 
        tcattcggt caccacaac catcaccac caccgtcac 
        ccataatat tatcacagg gcgtcaatg acatgcaac 
        aaccatctc tcattgacg cactggcag agccgtggg 
        ttgaagaga ggaggcagg gtgaagaga tggatgttg 
        aaaatgaca tcattcaac cctctcaat tgtcaccca 
        ttctagggc aaataaata ataggattg agaataggg 
        tggagggtg gagacgatg acgacgacg aagacatca 
        ttccaccca ctccccctc actcacgat caaggaggc 
        gtcggcgcc ctagaaagc ctcgtagac gttcgcgaa 
        agtcgccat taatattaa ttattaata attataata 
        ttaatatta atattaatg atgttaata ttaataatt 
        aatattaat aaatcctat ttccatcc
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY