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Gene information
General information
TC031638
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002089
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5931126..5933189
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031638
    iBeetle screen iB_06681

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031638 RA
      MALLGAQLV ITLVMISVI QKLGPHFSF ARWLLCSTG 
      LVRYLYPTD AELRHLASI PKDKLKGKK GPKPTNGKT 
      ETFHVPRSL DVQLETAKV TRLDVVHLR YYTEYQWLL 
      DFTVYAVIV YVVTEVYQT WFPLKDEVN LSMMWCGLV 
      LLFSVKLLL SLTVQYFKG EESVGERST CIVMGFTYL 
      LISMMVLIV DENTLELGL EDAYNSFNK SASAFLTTQ 
      GLNSSGPAS KIVLKFFIA LWCGILGAL FTFPGLRIA 
      KMHWDLLKY FRERKLIQI LLNLSFALP FVLVIFWIK 
      PVARTYLTV RVFSGMSQP LLTTHAFET LRITAIVFT 
      VLVKLALMP WYLQAYLDM AYNRTEEQK KEAGRITNI 
      EFQKKIAAV FYYLCVVTL QYITPIIMC LFLSFMYKT 
      LGEFTWSGL FKEPANECP VNSPNTKSI DPFESHGDS 
      IAQSAAEFH LAFENLKSV FSAEVFRGI FGFSTWWCC 
      FVYFATTSV GLVYQTYFS TI
    CDS sequence
    • >TC031638 RA
      atggccctg cttggcgcc caattggtc ataaccctg 
      gtcatgatc agcgtgatc cagaaacta gggccccac 
      ttttccttc gccaggtgg ctgctgtgc tccacgggg 
      ctcgtgagg tacttgtac cccacggat gccgagctg 
      cggcacctg gccagcatc cccaaagac aagctgaag 
      ggcaaaaag gggcccaag ccgactaat ggaaagacg 
      gagactttt catgttccg aggagtctc gacgtgcag 
      ttggagaca gccaaagtg acacgactt gacgttgtc 
      catttgaga tactataca gaatatcag tggttgctt 
      gattttaca gtctatgcg gttattgtc tatgttgtg 
      acggaggtg taccagacg tggttcccg ctcaaagat 
      gaggtcaat ttgagcatg atgtggtgc gggttggtg 
      ctccttttc tcggtgaaa ctgctccta tccctcaca 
      gtacagtac ttcaagggg gaggagtcg gttggtgaa 
      cgctccacg tgtattgtt atgggattc acctattta 
      ttaatctca atgatggtc ttgattgtt gatgaaaat 
      actctcgag ttaggtcta gaagatgcg tataacagt 
      tttaataaa agtgcgtcc gcttttttg accactcaa 
      ggcctaaat tcgtctgga ccggcgtct aaaatcgta 
      ttgaaattt tttattgcc ttgtggtgt ggtatttta 
      ggagcttta ttcactttt ccaggattg agaattgca 
      aaaatgcat tgggatttg cttaaatat ttcagggag 
      aggaaatta attcaaatt ttgttaaac ctaagtttt 
      gcgttacct tttgttttg gtgattttt tggatcaaa 
      ccagtggct aggacctac ctcacagtg cgggttttt 
      tccggaatg agccaacct ttgttgacg acacacgca 
      tttgaaacc ctccgaatc accgccata gtgtttaca 
      gttttggtc aagttggca ctgatgcca tggtaccta 
      caagcctac ttagacatg gcatacaac cgaactgaa 
      gaacaaaag aaggaagct ggccgtatc acaaatatc 
      gaatttcag aaaaaaatc gctgccgtt ttttactac 
      ctctgcgtg gtaaccctg cagtacatc acccccata 
      atcatgtgc ctgtttctc tccttcatg tacaagact 
      ttgggggaa ttcacgtgg agcggttta ttcaaagaa 
      ccagcgaac gaatgtccg gtaaattcc ccaaatacc 
      aaaagtatc gatccgttt gagtcacat ggtgactcg 
      atcgcccaa tcagcggcc gagtttcat ctcgctttc 
      gagaatttg aaatcggtc ttttcggcc gaagtgttt 
      cggggaatt tttggtttt tcgacttgg tggtgttgt 
      tttgtttat ttcgcaacg acgtcggta gggcttgtc 
      taccagacg tacttttcg acgatttga
    mRNA sequence
    • >TC031638 RA
      ctcaaactt gataacacc cagttcaag tcacatgtg 
      tgtaatgta atcaactta atcacacat ttagctcaa 
      ttgggcaaa atcgggtca attttgact cgcccccga 
      aaccttatg taaaaacgg tctaaaaat acgcccatt 
      tataggtta ggtcagcta tttctatta ataagacaa 
      tggtgtagt cacgtttgc acgctactg cgactatgg 
      ccctgcttg gcgcccaat tggtcataa ccctggtca 
      tgatcagcg tgatccaga aactagggc cccactttt 
      ccttcgcca ggtggctgc tgtgctcca cggggctcg 
      tgaggtact tgtacccca cggatgccg agctgcggc 
      acctggcca gcatcccca aagacaagc tgaagggca 
      aaaaggggc ccaagccga ctaatggaa agacggaga 
      cttttcatg ttccgagga gtctcgacg tgcagttgg 
      agacagcca aagtgacac gacttgacg ttgtccatt 
      tgagatact atacagaat atcagtggt tgcttgatt 
      ttacagtct atgcggtta ttgtctatg ttgtgacgg 
      aggtgtacc agacgtggt tcccgctca aagatgagg 
      tcaatttga gcatgatgt ggtgcgggt tggtgctcc 
      ttttctcgg tgaaactgc tcctatccc tcacagtac 
      agtacttca agggggagg agtcggttg gtgaacgct 
      ccacgtgta ttgttatgg gattcacct atttattaa 
      tctcaatga tggtcttga ttgttgatg aaaatactc 
      tcgagttag gtctagaag atgcgtata acagtttta 
      ataaaagtg cgtccgctt ttttgacca ctcaaggcc 
      taaattcgt ctggaccgg cgtctaaaa tcgtattga 
      aatttttta ttgccttgt ggtgtggta ttttaggag 
      ctttattca cttttccag gattgagaa ttgcaaaaa 
      tgcattggg atttgctta aatatttca gggagagga 
      aattaattc aaattttgt taaacctaa gttttgcgt 
      taccttttg ttttggtga ttttttgga tcaaaccag 
      tggctagga cctacctca cagtgcggg ttttttccg 
      gaatgagcc aacctttgt tgacgacac acgcatttg 
      aaaccctcc gaatcaccg ccatagtgt ttacagttt 
      tggtcaagt tggcactga tgccatggt acctacaag 
      cctacttag acatggcat acaaccgaa ctgaagaac 
      aaaagaagg aagctggcc gtatcacaa atatcgaat 
      ttcagaaaa aaatcgctg ccgtttttt actacctct 
      gcgtggtaa ccctgcagt acatcaccc ccataatca 
      tgtgcctgt ttctctcct tcatgtaca agactttgg 
      gggaattca cgtggagcg gtttattca aagaaccag 
      cgaacgaat gtccggtaa attccccaa ataccaaaa 
      gtatcgatc cgtttgagt cacatggtg actcgatcg 
      cccaatcag cggccgagt ttcatctcg ctttcgaga 
      atttgaaat cggtctttt cggccgaag tgtttcggg 
      gaatttttg gtttttcga cttggtggt gttgttttg 
      tttatttcg caacgacgt cggtagggc ttgtctacc 
      agacgtact tttcgacga tttgattgg tttgtgaat 
      aaataaaag gcgtggct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY