Status
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Gene information
General information
TC031075
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002060,TC002061
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5977391..5983305
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031075
    iBeetle screen iB_06677

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031075 RA
      MNAFQPCRN TSDCVPLYL PQQLYLKPA ARLTISIQL 
      PNIKKLEKS ISHWEIMEK LRTLIRPDE FSVLKVSKT 
      TLNFVRFEA ELENRTRLD RVVSKLDNN SIKLKEIPD 
      LLRLKASEV KSDFPTRHK WDSFFQEAK DMDEMKPGQ 
      RPDTVHLSN LPRKWFVPY HLPEGEDVV PSEKLFYRI 
      FEKFGQIRY VDIPVCDPY RKKMKDHIS GIKAHPVDD 
      RDFFEGYVQ FKDYIGFTK TMDALKGMK LVRKEEDEA 
      FCVNIKVDF DRSKHLSDA SIRRREIVR DRLVKKQRE 
      KEEKEKNEL DAKKSRELD ERQKEVTLK SEKEQRRRE 
      REEKRKARL LEKLKINSC DEINDKIAK EEKKLLKAQ 
      RKLESIRLV EELFRRIKE KKEELHMYS KTKSSSGDE 
      LKRFKDMSE LEVLTQRQK LHNAIEGRV VLKNILSRK 
      KKSRSRSSS SSDTSLSLD EDRKDRNIP EVVYDPTWY 
      NYPIYVYPP RGRYPLPGP SRGRPPFRG RGFRPRYRG 
      GFRGKYSHY SPEMEEQYM RYFSKFFSE HDRRSRSRS 
      HKRSRRSRS RSKSVDSSK FVSPRSSRR RSKSWSMPR 
      KCQEYKHRI KRRASVVTY IFGGSASRS REFPHMAAL 
      GYGQPIEWL CGGSLISER FVLTAAHCL ATSNLGELV 
      RVRLGDLDL QSVTDDAQP QDYRVSQKI IHPSYHAPA 
      QYDDIALIR LDRDVQFSP YIAPICLET QKNLPNYNF 
      IATGWGKTE VGGSQSDIL MKVDLEYFS NQICRQNYA 
      NVGSEYLSR GVDDNSQIC AGSRKDGKD TCQGDSGGP 
      LQIRTDVLY LVGITSFGK ICGIPNSPG VYTRVSYYI 
      PWIERIVWP Q
    • >TC031075 RB
      MNAFQPCRN TSDCVPLYL PQQLYLKPA ARLTISIQL 
      PNIKKLEKS ISHWEIMEK LRTLIRPDE FSVLKVSKT 
      TLNFVRFEA ELENRTRLD RVVSKLDNN SIKLKEIPD 
      LLRLKASEV KSDFPTRHK WDSFFQEAK DMDEMKPGQ 
      RPDTVHLSN LPRKWFVPY HLPEGEDVV PSEKLFYRI 
      FEKFGQIRY VDIPVCDPY RKKMKDHIS GIKAHPVDD 
      RDFFEGYVQ FKDYIGFTK TMDALKGMK LVRKEEDEA 
      FCVNIKVDF DRSKHLSDA SIRRREIVR DRLVKKQRE 
      KEEKEKNEL DAKKSRELD ERQKEVTLK SEKEQRRRE 
      REEKRKARL LEKLKINSC DEINDKIAK EEKKLLKAQ 
      RKLESIRLV EELFRRIKE KKEELHMYS KTKSSSGDE 
      LKRFKDMSE LEVLTQRQK LHNAIEGRV VLKNILSRK 
      KKSRSRSSS SSDTSLSLD EDRKDRNIP EVVYDPTWY 
      NYPIYVYPP RGRYPLPGP SRGRPPFRG RGFRPRYRG 
      GFRGKYSHY SPEMEEQYM RYFSKFFSE HDRRSRSRS 
      KSVDSSKFV SPRSSRRRS KSWSMPRSG GEKGRSWSK 
      TPSQSGKTP KIARHKITG FCNSGPHHL GKNIVTPTI 
      TPSPIVQTV RPTTNPIPV PTIPTASVA TTKCQEYKH 
      RIKRRASVV TYIFGGSAS RSREFPHMA ALGYGQPIE 
      WLCGGSLIS ERFVLTAAH CLATSNLGE LVRVRLGDL 
      DLQSVTDDA QPQDYRVSQ KIIHPSYHA PAQYDDIAL 
      IRLDRDVQF SPYIAPICL ETQKNLPNY NFIATGWGK 
      TEVGGSQSD ILMKVDLEY FSNQICRQN YANVGSEYL 
      SRGVDDNSQ ICAGSRKDG KDTCQGDSG GPLQIRTDV 
      LYLVGITSF GKICGIPNS PGVYTRVSY YIPWIERIV 
      WPQ
    • >TC031075 RC
      MNAFQPCRN TSDCVPLYL PQQLYLKPA ARLTISIQL 
      PNIKKLEKS ISHWEIMEK LRTLIRPDE FSVLKVSKT 
      TLNFVRFEA ELENRTRLD RVVSKLDNN SIKLKEIPD 
      LLRLKASEV KSDFPTRHK WDSFFQEAK DMDEMKPGQ 
      RPDTVHLSN LPRKWFVPY HLPEGEDVV PSEKLFYRI 
      FEKFGQIRY VDIPVCDPY RKKMKDHIS GIKAHPVDD 
      RDFFEGYVQ FKDYIGFTK TMDALKGMK LVRKEEDEA 
      FCVNIKVDF DRSKHLSDA SIRRREIVR DRLVKKQRE 
      KEEKEKNEL DAKKSRELD ERQKEVTLK SEKEQRRRE 
      REEKRKARL LEKLKINSC DEINDKIAK EEKKLLKAQ 
      RKLESIRLV EELFRRIKE KKEELHMYS KTKSSSGDE 
      LKRFKDMSE LEVLTQRQK LHNAIEGRV VLKNILSRK 
      KKSRSRSSS SSDTSLSLD EDRKDRNIP EVVYDPTWY 
      NYPIYVYPP RGRYPLPGP SRGRPPFRG RGFRPRYRG 
      GFRGKYSHY SPEMEEQYM RYFSKFFSE HDRRSRSRS 
      HKRSRRSRS RSKSVDSSK FVSPRSSRR RSKSWSMPR 
      SGGEKGRSW SKTPSQSGK TPKIARHKI TGFCNSGPH 
      HLGKNIVTP TITPSPIVQ TVRPTTNPI PVPTIPTAS 
      VATTKCQEY KHRIKRRAS VVTYIFGGS ASRSREFPH 
      MAALGYGQP IEWLCGGSL ISERFVLTA AHCLATSNL 
      GELVRVRLG DLDLQSVTD DAQPQDYRV SQKIIHPSY 
      HAPAQYDDI ALIRLDRDV QFSPYIAPI CLETQKNLP 
      NYNFIATGW GKTEVGGSQ SDILMKVDL EYFSNQICR 
      QNYANVGSE YLSRGVDDN SQICAGSRK DGKDTCQGD 
      SGGPLQIRT DVLYLVGIT SFGKICGIP NSPGVYTRV 
      SYYIPWIER IVWPQ
    CDS sequence
    • >TC031075 RA
      atgaatgca tttcagcca tgtcgcaac acctccgac 
      tgcgtgccc ctctacctg ccccagcag ctgtacctg 
      aaaccggcg gctcgtttg accatttcc atccaactc 
      cccaacata aaaaagcta gaaaaaagc atctcccac 
      tgggaaatc atggaaaag ttgcgcact ttgatccgt 
      cctgacgaa ttcagcgtg ttaaaagtg tccaagact 
      acgctcaat ttcgtccga tttgaggcc gagctggag 
      aatcggaca cgtctggat cgagttgtg tccaaactg 
      gacaacaat tcgatcaaa ctgaaggaa attcccgac 
      ttgttgcgc cttaaagcg agcgaagtt aagagcgat 
      tttcccacg agacataaa tgggactca tttttccaa 
      gaagctaaa gacatggat gagatgaaa ccggggcaa 
      aggcctgac actgtacac ctgtcgaat ttgccccgg 
      aaatggttc gttccgtac catttgccc gagggcgag 
      gatgtcgtc ccttctgag aaattattt taccggatt 
      tttgaaaag ttcgggcaa atccggtac gtggatatt 
      ccggtttgt gatccttac cggaaaaaa atgaaggac 
      cacatttcc gggattaag gcacatccg gtcgacgat 
      cgcgatttt ttcgagggt tacgtccaa tttaaggac 
      tacattggc tttactaaa actatggac gcccttaaa 
      gggatgaaa ctagtgcgc aaggaggaa gatgaggca 
      ttttgtgtc aatatcaag gttgatttc gatcgttcg 
      aaacatctg agtgatgcc tcaatacgt cggagagag 
      atagtaaga gacagactg gtcaaaaaa caacgagag 
      aaagaagaa aaggagaaa aatgagctt gatgcgaaa 
      aaatcaaga gaactagac gagaggcaa aaagaggtg 
      accttgaag agcgaaaag gagcaaaga cggcgcgaa 
      cgcgaagaa aaacgcaaa gctagactt ttggaaaag 
      ctcaaaatc aacagttgt gacgaaatt aacgataaa 
      atcgccaaa gaagagaaa aaattgctt aaagcgcaa 
      cgtaaactc gagtcaatt cgactcgtt gaagaacta 
      ttcagacgt attaaggag aaaaaagaa gagttgcat 
      atgtacagt aaaacgaaa agttcaagt ggcgatgag 
      ctcaaaaga ttcaaagat atgagcgaa ttggaggtt 
      ttaacccaa cgacaaaaa ctacacaat gcgattgag 
      ggccgagtt gtattgaaa aatatcttg tcgaggaaa 
      aaaaaatca aggagtcgc tcatcgtcc agttctgac 
      acttcccta tcgcttgat gaggaccga aaagaccgc 
      aatattcct gaggttgtt tacgacccc acgtggtac 
      aactacccc atctacgtg tacccccct cgaggtcgg 
      taccccctt ccggggccc tcccgtggg cgtcccccc 
      tttcggggg cgcggtttc cggccgcgc tatcgtggg 
      ggttttcgg gggaagtac tcacactat tcccccgaa 
      atggaggaa cagtatatg cgatacttt tccaagttt 
      ttttcggaa catgacagg aggtcaagg tcaaggtcg 
      cacaagagg tcacgaagg tcaaggtca aggtcgaaa 
      agtgtggat agttccaag ttcgtgtcg ccgaggagt 
      tcgaggagg aggtcgaag tcgtggtcc atgccgagg 
      aaatgtcaa gagtacaaa caccgaatt aaaagacgc 
      gcctctgtt gtgacatac atttttggc gggagtgcg 
      tcccgaagc cgggaattc ccccacatg gcggccttg 
      ggctacggc caaccaatc gaatggctt tgcggtggt 
      agtttaatt agcgaacgc tttgtccta acagccgct 
      cattgccta gcaacgtcc aacctgggc gagttggtg 
      agagttaga ttgggtgac cttgacctc caaagtgtc 
      actgacgac gcccaaccc caggattac agagtgtcc 
      caaaaaata atacatccc agttaccac gcgcctgcg 
      cagtatgac gatatagcc ctgattagg ctggacagg 
      gatgtccaa ttcagccca tatattgcg cccatttgt 
      ttggagact cagaagaac ttgcccaat tataatttt 
      attgccaca gggtggggg aagaccgaa gtcgggggt 
      tcccagagc gatattttg atgaaagtg gatttggag 
      tatttttcg aatcaaatt tgccgccaa aattacgcc 
      aacgttggg agtgaatat ctctcaagg ggggttgat 
      gacaacagc cagatttgt gcaggaagt cgcaaggat 
      gggaaggac acgtgtcag ggtgattcc gggggtccc 
      ctgcaaatc cgtaccgac gtcctgtac ttagtcggg 
      atcacgtcc tttggaaaa atctgtggc atcccaaac 
      agtccgggg gtttacacg agagtctcg tattacata 
      ccgtggatc gaacgaatt gtttggccc caataa
    • >TC031075 RB
      atgaatgca tttcagcca tgtcgcaac acctccgac 
      tgcgtgccc ctctacctg ccccagcag ctgtacctg 
      aaaccggcg gctcgtttg accatttcc atccaactc 
      cccaacata aaaaagcta gaaaaaagc atctcccac 
      tgggaaatc atggaaaag ttgcgcact ttgatccgt 
      cctgacgaa ttcagcgtg ttaaaagtg tccaagact 
      acgctcaat ttcgtccga tttgaggcc gagctggag 
      aatcggaca cgtctggat cgagttgtg tccaaactg 
      gacaacaat tcgatcaaa ctgaaggaa attcccgac 
      ttgttgcgc cttaaagcg agcgaagtt aagagcgat 
      tttcccacg agacataaa tgggactca tttttccaa 
      gaagctaaa gacatggat gagatgaaa ccggggcaa 
      aggcctgac actgtacac ctgtcgaat ttgccccgg 
      aaatggttc gttccgtac catttgccc gagggcgag 
      gatgtcgtc ccttctgag aaattattt taccggatt 
      tttgaaaag ttcgggcaa atccggtac gtggatatt 
      ccggtttgt gatccttac cggaaaaaa atgaaggac 
      cacatttcc gggattaag gcacatccg gtcgacgat 
      cgcgatttt ttcgagggt tacgtccaa tttaaggac 
      tacattggc tttactaaa actatggac gcccttaaa 
      gggatgaaa ctagtgcgc aaggaggaa gatgaggca 
      ttttgtgtc aatatcaag gttgatttc gatcgttcg 
      aaacatctg agtgatgcc tcaatacgt cggagagag 
      atagtaaga gacagactg gtcaaaaaa caacgagag 
      aaagaagaa aaggagaaa aatgagctt gatgcgaaa 
      aaatcaaga gaactagac gagaggcaa aaagaggtg 
      accttgaag agcgaaaag gagcaaaga cggcgcgaa 
      cgcgaagaa aaacgcaaa gctagactt ttggaaaag 
      ctcaaaatc aacagttgt gacgaaatt aacgataaa 
      atcgccaaa gaagagaaa aaattgctt aaagcgcaa 
      cgtaaactc gagtcaatt cgactcgtt gaagaacta 
      ttcagacgt attaaggag aaaaaagaa gagttgcat 
      atgtacagt aaaacgaaa agttcaagt ggcgatgag 
      ctcaaaaga ttcaaagat atgagcgaa ttggaggtt 
      ttaacccaa cgacaaaaa ctacacaat gcgattgag 
      ggccgagtt gtattgaaa aatatcttg tcgaggaaa 
      aaaaaatca aggagtcgc tcatcgtcc agttctgac 
      acttcccta tcgcttgat gaggaccga aaagaccgc 
      aatattcct gaggttgtt tacgacccc acgtggtac 
      aactacccc atctacgtg tacccccct cgaggtcgg 
      taccccctt ccggggccc tcccgtggg cgtcccccc 
      tttcggggg cgcggtttc cggccgcgc tatcgtggg 
      ggttttcgg gggaagtac tcacactat tcccccgaa 
      atggaggaa cagtatatg cgatacttt tccaagttt 
      ttttcggaa catgacagg aggtcaagg tcaaggtcg 
      aaaagtgtg gatagttcc aagttcgtg tcgccgagg 
      agttcgagg aggaggtcg aagtcgtgg tccatgccg 
      aggagtggg ggggagaag gggaggtcg tggtcgaag 
      acgccttca caatcaggt aaaaccccc aaaattgcc 
      agacacaaa attacaggt ttctgtaat tcagggccg 
      caccatttg ggcaaaaat atcgtcacc ccaaccata 
      accccgagc cccatcgtc caaacagtt cgaccaact 
      acaaaccca atcccggta ccaaccatc ccgacggcc 
      agtgtcgcc accacaaaa tgtcaagag tacaaacac 
      cgaattaaa agacgcgcc tctgttgtg acatacatt 
      tttggcggg agtgcgtcc cgaagccgg gaattcccc 
      cacatggcg gccttgggc tacggccaa ccaatcgaa 
      tggctttgc ggtggtagt ttaattagc gaacgcttt 
      gtcctaaca gccgctcat tgcctagca acgtccaac 
      ctgggcgag ttggtgaga gttagattg ggtgacctt 
      gacctccaa agtgtcact gacgacgcc caaccccag 
      gattacaga gtgtcccaa aaaataata catcccagt 
      taccacgcg cctgcgcag tatgacgat atagccctg 
      attaggctg gacagggat gtccaattc agcccatat 
      attgcgccc atttgtttg gagactcag aagaacttg 
      cccaattat aattttatt gccacaggg tgggggaag 
      accgaagtc gggggttcc cagagcgat attttgatg 
      aaagtggat ttggagtat ttttcgaat caaatttgc 
      cgccaaaat tacgccaac gttgggagt gaatatctc 
      tcaaggggg gttgatgac aacagccag atttgtgca 
      ggaagtcgc aaggatggg aaggacacg tgtcagggt 
      gattccggg ggtcccctg caaatccgt accgacgtc 
      ctgtactta gtcgggatc acgtccttt ggaaaaatc 
      tgtggcatc ccaaacagt ccgggggtt tacacgaga 
      gtctcgtat tacataccg tggatcgaa cgaattgtt 
      tggccccaa taa
    • >TC031075 RC
      atgaatgca tttcagcca tgtcgcaac acctccgac 
      tgcgtgccc ctctacctg ccccagcag ctgtacctg 
      aaaccggcg gctcgtttg accatttcc atccaactc 
      cccaacata aaaaagcta gaaaaaagc atctcccac 
      tgggaaatc atggaaaag ttgcgcact ttgatccgt 
      cctgacgaa ttcagcgtg ttaaaagtg tccaagact 
      acgctcaat ttcgtccga tttgaggcc gagctggag 
      aatcggaca cgtctggat cgagttgtg tccaaactg 
      gacaacaat tcgatcaaa ctgaaggaa attcccgac 
      ttgttgcgc cttaaagcg agcgaagtt aagagcgat 
      tttcccacg agacataaa tgggactca tttttccaa 
      gaagctaaa gacatggat gagatgaaa ccggggcaa 
      aggcctgac actgtacac ctgtcgaat ttgccccgg 
      aaatggttc gttccgtac catttgccc gagggcgag 
      gatgtcgtc ccttctgag aaattattt taccggatt 
      tttgaaaag ttcgggcaa atccggtac gtggatatt 
      ccggtttgt gatccttac cggaaaaaa atgaaggac 
      cacatttcc gggattaag gcacatccg gtcgacgat 
      cgcgatttt ttcgagggt tacgtccaa tttaaggac 
      tacattggc tttactaaa actatggac gcccttaaa 
      gggatgaaa ctagtgcgc aaggaggaa gatgaggca 
      ttttgtgtc aatatcaag gttgatttc gatcgttcg 
      aaacatctg agtgatgcc tcaatacgt cggagagag 
      atagtaaga gacagactg gtcaaaaaa caacgagag 
      aaagaagaa aaggagaaa aatgagctt gatgcgaaa 
      aaatcaaga gaactagac gagaggcaa aaagaggtg 
      accttgaag agcgaaaag gagcaaaga cggcgcgaa 
      cgcgaagaa aaacgcaaa gctagactt ttggaaaag 
      ctcaaaatc aacagttgt gacgaaatt aacgataaa 
      atcgccaaa gaagagaaa aaattgctt aaagcgcaa 
      cgtaaactc gagtcaatt cgactcgtt gaagaacta 
      ttcagacgt attaaggag aaaaaagaa gagttgcat 
      atgtacagt aaaacgaaa agttcaagt ggcgatgag 
      ctcaaaaga ttcaaagat atgagcgaa ttggaggtt 
      ttaacccaa cgacaaaaa ctacacaat gcgattgag 
      ggccgagtt gtattgaaa aatatcttg tcgaggaaa 
      aaaaaatca aggagtcgc tcatcgtcc agttctgac 
      acttcccta tcgcttgat gaggaccga aaagaccgc 
      aatattcct gaggttgtt tacgacccc acgtggtac 
      aactacccc atctacgtg tacccccct cgaggtcgg 
      taccccctt ccggggccc tcccgtggg cgtcccccc 
      tttcggggg cgcggtttc cggccgcgc tatcgtggg 
      ggttttcgg gggaagtac tcacactat tcccccgaa 
      atggaggaa cagtatatg cgatacttt tccaagttt 
      ttttcggaa catgacagg aggtcaagg tcaaggtcg 
      cacaagagg tcacgaagg tcaaggtca aggtcgaaa 
      agtgtggat agttccaag ttcgtgtcg ccgaggagt 
      tcgaggagg aggtcgaag tcgtggtcc atgccgagg 
      agtgggggg gagaagggg aggtcgtgg tcgaagacg 
      ccttcacaa tcaggtaaa acccccaaa attgccaga 
      cacaaaatt acaggtttc tgtaattca gggccgcac 
      catttgggc aaaaatatc gtcacccca accataacc 
      ccgagcccc atcgtccaa acagttcga ccaactaca 
      aacccaatc ccggtacca accatcccg acggccagt 
      gtcgccacc acaaaatgt caagagtac aaacaccga 
      attaaaaga cgcgcctct gttgtgaca tacattttt 
      ggcgggagt gcgtcccga agccgggaa ttcccccac 
      atggcggcc ttgggctac ggccaacca atcgaatgg 
      ctttgcggt ggtagttta attagcgaa cgctttgtc 
      ctaacagcc gctcattgc ctagcaacg tccaacctg 
      ggcgagttg gtgagagtt agattgggt gaccttgac 
      ctccaaagt gtcactgac gacgcccaa ccccaggat 
      tacagagtg tcccaaaaa ataatacat cccagttac 
      cacgcgcct gcgcagtat gacgatata gccctgatt 
      aggctggac agggatgtc caattcagc ccatatatt 
      gcgcccatt tgtttggag actcagaag aacttgccc 
      aattataat tttattgcc acagggtgg gggaagacc 
      gaagtcggg ggttcccag agcgatatt ttgatgaaa 
      gtggatttg gagtatttt tcgaatcaa atttgccgc 
      caaaattac gccaacgtt gggagtgaa tatctctca 
      aggggggtt gatgacaac agccagatt tgtgcagga 
      agtcgcaag gatgggaag gacacgtgt cagggtgat 
      tccgggggt cccctgcaa atccgtacc gacgtcctg 
      tacttagtc gggatcacg tcctttgga aaaatctgt 
      ggcatccca aacagtccg ggggtttac acgagagtc 
      tcgtattac ataccgtgg atcgaacga attgtttgg 
      ccccaataa
    mRNA sequence
    • >TC031075 RA
      aactttaac aaactagtt gagtttaat gaataattg 
      gtattctga atataggtt gtttggaac ggttgtatt 
      ggtagtatg gacgtcaag cgtaacttt aagtaaagt 
      ttttagcgt gattttccg cggacatgt ctcaatttt 
      ccagtactt tggttaaaa caacccaat gctagtctt 
      tcgtgtcta aatggccct attaagtaa ttcgggtcg 
      gttccgttt gtaaatctg gtgatttcg gccgccatc 
      ttgccttgt caaaagtga cactgtcag agtagtgag 
      gttaaattg tttatttcg gtgattaaa ggcatttat 
      gatagagag gagcccccc gtggttgcc cccctctca 
      tcatgaatg catttcagc catgtcgca acacctccg 
      actgcgtgc ccctctacc tgccccagc agctgtacc 
      tgaaaccgg cggctcgtt tgaccattt ccatccaac 
      tccccaaca taaaaaagc tagaaaaaa gcatctccc 
      actgggaaa tcatggaaa agttgcgca ctttgatcc 
      gtcctgacg aattcagcg tgttaaaag tgtccaaga 
      ctacgctca atttcgtcc gatttgagg ccgagctgg 
      agaatcgga cacgtctgg atcgagttg tgtccaaac 
      tggacaaca attcgatca aactgaagg aaattcccg 
      acttgttgc gccttaaag cgagcgaag ttaagagcg 
      attttccca cgagacata aatgggact catttttcc 
      aagaagcta aagacatgg atgagatga aaccggggc 
      aaaggcctg acactgtac acctgtcga atttgcccc 
      ggaaatggt tcgttccgt accatttgc ccgagggcg 
      aggatgtcg tcccttctg agaaattat tttaccgga 
      tttttgaaa agttcgggc aaatccggt acgtggata 
      ttccggttt gtgatcctt accggaaaa aaatgaagg 
      accacattt ccgggatta aggcacatc cggtcgacg 
      atcgcgatt ttttcgagg gttacgtcc aatttaagg 
      actacattg gctttacta aaactatgg acgccctta 
      aagggatga aactagtgc gcaaggagg aagatgagg 
      cattttgtg tcaatatca aggttgatt tcgatcgtt 
      cgaaacatc tgagtgatg cctcaatac gtcggagag 
      agatagtaa gagacagac tggtcaaaa aacaacgag 
      agaaagaag aaaaggaga aaaatgagc ttgatgcga 
      aaaaatcaa gagaactag acgagaggc aaaaagagg 
      tgaccttga agagcgaaa aggagcaaa gacggcgcg 
      aacgcgaag aaaaacgca aagctagac ttttggaaa 
      agctcaaaa tcaacagtt gtgacgaaa ttaacgata 
      aaatcgcca aagaagaga aaaaattgc ttaaagcgc 
      aacgtaaac tcgagtcaa ttcgactcg ttgaagaac 
      tattcagac gtattaagg agaaaaaag aagagttgc 
      atatgtaca gtaaaacga aaagttcaa gtggcgatg 
      agctcaaaa gattcaaag atatgagcg aattggagg 
      ttttaaccc aacgacaaa aactacaca atgcgattg 
      agggccgag ttgtattga aaaatatct tgtcgagga 
      aaaaaaaat caaggagtc gctcatcgt ccagttctg 
      acacttccc tatcgcttg atgaggacc gaaaagacc 
      gcaatattc ctgaggttg tttacgacc ccacgtggt 
      acaactacc ccatctacg tgtaccccc ctcgaggtc 
      ggtaccccc ttccggggc cctcccgtg ggcgtcccc 
      cctttcggg ggcgcggtt tccggccgc gctatcgtg 
      ggggttttc gggggaagt actcacact attcccccg 
      aaatggagg aacagtata tgcgatact tttccaagt 
      ttttttcgg aacatgaca ggaggtcaa ggtcaaggt 
      cgcacaaga ggtcacgaa ggtcaaggt caaggtcga 
      aaagtgtgg atagttcca agttcgtgt cgccgagga 
      gttcgagga ggaggtcga agtcgtggt ccatgccga 
      ggaaatgtc aagagtaca aacaccgaa ttaaaagac 
      gcgcctctg ttgtgacat acatttttg gcgggagtg 
      cgtcccgaa gccgggaat tcccccaca tggcggcct 
      tgggctacg gccaaccaa tcgaatggc tttgcggtg 
      gtagtttaa ttagcgaac gctttgtcc taacagccg 
      ctcattgcc tagcaacgt ccaacctgg gcgagttgg 
      tgagagtta gattgggtg accttgacc tccaaagtg 
      tcactgacg acgcccaac cccaggatt acagagtgt 
      cccaaaaaa taatacatc ccagttacc acgcgcctg 
      cgcagtatg acgatatag ccctgatta ggctggaca 
      gggatgtcc aattcagcc catatattg cgcccattt 
      gtttggaga ctcagaaga acttgccca attataatt 
      ttattgcca cagggtggg ggaagaccg aagtcgggg 
      gttcccaga gcgatattt tgatgaaag tggatttgg 
      agtattttt cgaatcaaa tttgccgcc aaaattacg 
      ccaacgttg ggagtgaat atctctcaa ggggggttg 
      atgacaaca gccagattt gtgcaggaa gtcgcaagg 
      atgggaagg acacgtgtc agggtgatt ccgggggtc 
      ccctgcaaa tccgtaccg acgtcctgt acttagtcg 
      ggatcacgt cctttggaa aaatctgtg gcatcccaa 
      acagtccgg gggtttaca cgagagtct cgtattaca 
      taccgtgga tcgaacgaa ttgtttggc cccaataat 
      acagttaaa taaattata accttaaaa
    • >TC031075 RB
      aactttaac aaactagtt gagtttaat gaataattg 
      gtattctga atataggtt gtttggaac ggttgtatt 
      ggtagtatg gacgtcaag cgtaacttt aagtaaagt 
      ttttagcgt gattttccg cggacatgt ctcaatttt 
      ccagtactt tggttaaaa caacccaat gctagtctt 
      tcgtgtcta aatggccct attaagtaa ttcgggtcg 
      gttccgttt gtaaatctg gtgatttcg gccgccatc 
      ttgccttgt caaaagtga cactgtcag agtagtgag 
      gttaaattg tttatttcg gtgattaaa ggcatttat 
      gatagagag gagcccccc gtggttgcc cccctctca 
      tcatgaatg catttcagc catgtcgca acacctccg 
      actgcgtgc ccctctacc tgccccagc agctgtacc 
      tgaaaccgg cggctcgtt tgaccattt ccatccaac 
      tccccaaca taaaaaagc tagaaaaaa gcatctccc 
      actgggaaa tcatggaaa agttgcgca ctttgatcc 
      gtcctgacg aattcagcg tgttaaaag tgtccaaga 
      ctacgctca atttcgtcc gatttgagg ccgagctgg 
      agaatcgga cacgtctgg atcgagttg tgtccaaac 
      tggacaaca attcgatca aactgaagg aaattcccg 
      acttgttgc gccttaaag cgagcgaag ttaagagcg 
      attttccca cgagacata aatgggact catttttcc 
      aagaagcta aagacatgg atgagatga aaccggggc 
      aaaggcctg acactgtac acctgtcga atttgcccc 
      ggaaatggt tcgttccgt accatttgc ccgagggcg 
      aggatgtcg tcccttctg agaaattat tttaccgga 
      tttttgaaa agttcgggc aaatccggt acgtggata 
      ttccggttt gtgatcctt accggaaaa aaatgaagg 
      accacattt ccgggatta aggcacatc cggtcgacg 
      atcgcgatt ttttcgagg gttacgtcc aatttaagg 
      actacattg gctttacta aaactatgg acgccctta 
      aagggatga aactagtgc gcaaggagg aagatgagg 
      cattttgtg tcaatatca aggttgatt tcgatcgtt 
      cgaaacatc tgagtgatg cctcaatac gtcggagag 
      agatagtaa gagacagac tggtcaaaa aacaacgag 
      agaaagaag aaaaggaga aaaatgagc ttgatgcga 
      aaaaatcaa gagaactag acgagaggc aaaaagagg 
      tgaccttga agagcgaaa aggagcaaa gacggcgcg 
      aacgcgaag aaaaacgca aagctagac ttttggaaa 
      agctcaaaa tcaacagtt gtgacgaaa ttaacgata 
      aaatcgcca aagaagaga aaaaattgc ttaaagcgc 
      aacgtaaac tcgagtcaa ttcgactcg ttgaagaac 
      tattcagac gtattaagg agaaaaaag aagagttgc 
      atatgtaca gtaaaacga aaagttcaa gtggcgatg 
      agctcaaaa gattcaaag atatgagcg aattggagg 
      ttttaaccc aacgacaaa aactacaca atgcgattg 
      agggccgag ttgtattga aaaatatct tgtcgagga 
      aaaaaaaat caaggagtc gctcatcgt ccagttctg 
      acacttccc tatcgcttg atgaggacc gaaaagacc 
      gcaatattc ctgaggttg tttacgacc ccacgtggt 
      acaactacc ccatctacg tgtaccccc ctcgaggtc 
      ggtaccccc ttccggggc cctcccgtg ggcgtcccc 
      cctttcggg ggcgcggtt tccggccgc gctatcgtg 
      ggggttttc gggggaagt actcacact attcccccg 
      aaatggagg aacagtata tgcgatact tttccaagt 
      ttttttcgg aacatgaca ggaggtcaa ggtcaaggt 
      cgaaaagtg tggatagtt ccaagttcg tgtcgccga 
      ggagttcga ggaggaggt cgaagtcgt ggtccatgc 
      cgaggagtg ggggggaga aggggaggt cgtggtcga 
      agacgcctt cacaatcag gtaaaaccc ccaaaattg 
      ccagacaca aaattacag gtttctgta attcagggc 
      cgcaccatt tgggcaaaa atatcgtca ccccaacca 
      taaccccga gccccatcg tccaaacag ttcgaccaa 
      ctacaaacc caatcccgg taccaacca tcccgacgg 
      ccagtgtcg ccaccacaa aatgtcaag agtacaaac 
      accgaatta aaagacgcg cctctgttg tgacataca 
      tttttggcg ggagtgcgt cccgaagcc gggaattcc 
      cccacatgg cggccttgg gctacggcc aaccaatcg 
      aatggcttt gcggtggta gtttaatta gcgaacgct 
      ttgtcctaa cagccgctc attgcctag caacgtcca 
      acctgggcg agttggtga gagttagat tgggtgacc 
      ttgacctcc aaagtgtca ctgacgacg cccaacccc 
      aggattaca gagtgtccc aaaaaataa tacatccca 
      gttaccacg cgcctgcgc agtatgacg atatagccc 
      tgattaggc tggacaggg atgtccaat tcagcccat 
      atattgcgc ccatttgtt tggagactc agaagaact 
      tgcccaatt ataatttta ttgccacag ggtggggga 
      agaccgaag tcgggggtt cccagagcg atattttga 
      tgaaagtgg atttggagt atttttcga atcaaattt 
      gccgccaaa attacgcca acgttggga gtgaatatc 
      tctcaaggg gggttgatg acaacagcc agatttgtg 
      caggaagtc gcaaggatg ggaaggaca cgtgtcagg 
      gtgattccg ggggtcccc tgcaaatcc gtaccgacg 
      tcctgtact tagtcggga tcacgtcct ttggaaaaa 
      tctgtggca tcccaaaca gtccggggg tttacacga 
      gagtctcgt attacatac cgtggatcg aacgaattg 
      tttggcccc aataataca gttaaataa attataacc 
      ttaaaa
    • >TC031075 RC
      aactttaac aaactagtt gagtttaat gaataattg 
      gtattctga atataggtt gtttggaac ggttgtatt 
      ggtagtatg gacgtcaag cgtaacttt aagtaaagt 
      ttttagcgt gattttccg cggacatgt ctcaatttt 
      ccagtactt tggttaaaa caacccaat gctagtctt 
      tcgtgtcta aatggccct attaagtaa ttcgggtcg 
      gttccgttt gtaaatctg gtgatttcg gccgccatc 
      ttgccttgt caaaagtga cactgtcag agtagtgag 
      gttaaattg tttatttcg gtgattaaa ggcatttat 
      gatagagag gagcccccc gtggttgcc cccctctca 
      tcatgaatg catttcagc catgtcgca acacctccg 
      actgcgtgc ccctctacc tgccccagc agctgtacc 
      tgaaaccgg cggctcgtt tgaccattt ccatccaac 
      tccccaaca taaaaaagc tagaaaaaa gcatctccc 
      actgggaaa tcatggaaa agttgcgca ctttgatcc 
      gtcctgacg aattcagcg tgttaaaag tgtccaaga 
      ctacgctca atttcgtcc gatttgagg ccgagctgg 
      agaatcgga cacgtctgg atcgagttg tgtccaaac 
      tggacaaca attcgatca aactgaagg aaattcccg 
      acttgttgc gccttaaag cgagcgaag ttaagagcg 
      attttccca cgagacata aatgggact catttttcc 
      aagaagcta aagacatgg atgagatga aaccggggc 
      aaaggcctg acactgtac acctgtcga atttgcccc 
      ggaaatggt tcgttccgt accatttgc ccgagggcg 
      aggatgtcg tcccttctg agaaattat tttaccgga 
      tttttgaaa agttcgggc aaatccggt acgtggata 
      ttccggttt gtgatcctt accggaaaa aaatgaagg 
      accacattt ccgggatta aggcacatc cggtcgacg 
      atcgcgatt ttttcgagg gttacgtcc aatttaagg 
      actacattg gctttacta aaactatgg acgccctta 
      aagggatga aactagtgc gcaaggagg aagatgagg 
      cattttgtg tcaatatca aggttgatt tcgatcgtt 
      cgaaacatc tgagtgatg cctcaatac gtcggagag 
      agatagtaa gagacagac tggtcaaaa aacaacgag 
      agaaagaag aaaaggaga aaaatgagc ttgatgcga 
      aaaaatcaa gagaactag acgagaggc aaaaagagg 
      tgaccttga agagcgaaa aggagcaaa gacggcgcg 
      aacgcgaag aaaaacgca aagctagac ttttggaaa 
      agctcaaaa tcaacagtt gtgacgaaa ttaacgata 
      aaatcgcca aagaagaga aaaaattgc ttaaagcgc 
      aacgtaaac tcgagtcaa ttcgactcg ttgaagaac 
      tattcagac gtattaagg agaaaaaag aagagttgc 
      atatgtaca gtaaaacga aaagttcaa gtggcgatg 
      agctcaaaa gattcaaag atatgagcg aattggagg 
      ttttaaccc aacgacaaa aactacaca atgcgattg 
      agggccgag ttgtattga aaaatatct tgtcgagga 
      aaaaaaaat caaggagtc gctcatcgt ccagttctg 
      acacttccc tatcgcttg atgaggacc gaaaagacc 
      gcaatattc ctgaggttg tttacgacc ccacgtggt 
      acaactacc ccatctacg tgtaccccc ctcgaggtc 
      ggtaccccc ttccggggc cctcccgtg ggcgtcccc 
      cctttcggg ggcgcggtt tccggccgc gctatcgtg 
      ggggttttc gggggaagt actcacact attcccccg 
      aaatggagg aacagtata tgcgatact tttccaagt 
      ttttttcgg aacatgaca ggaggtcaa ggtcaaggt 
      cgcacaaga ggtcacgaa ggtcaaggt caaggtcga 
      aaagtgtgg atagttcca agttcgtgt cgccgagga 
      gttcgagga ggaggtcga agtcgtggt ccatgccga 
      ggagtgggg gggagaagg ggaggtcgt ggtcgaaga 
      cgccttcac aatcaggta aaaccccca aaattgcca 
      gacacaaaa ttacaggtt tctgtaatt cagggccgc 
      accatttgg gcaaaaata tcgtcaccc caaccataa 
      ccccgagcc ccatcgtcc aaacagttc gaccaacta 
      caaacccaa tcccggtac caaccatcc cgacggcca 
      gtgtcgcca ccacaaaat gtcaagagt acaaacacc 
      gaattaaaa gacgcgcct ctgttgtga catacattt 
      ttggcggga gtgcgtccc gaagccggg aattccccc 
      acatggcgg ccttgggct acggccaac caatcgaat 
      ggctttgcg gtggtagtt taattagcg aacgctttg 
      tcctaacag ccgctcatt gcctagcaa cgtccaacc 
      tgggcgagt tggtgagag ttagattgg gtgaccttg 
      acctccaaa gtgtcactg acgacgccc aaccccagg 
      attacagag tgtcccaaa aaataatac atcccagtt 
      accacgcgc ctgcgcagt atgacgata tagccctga 
      ttaggctgg acagggatg tccaattca gcccatata 
      ttgcgccca tttgtttgg agactcaga agaacttgc 
      ccaattata attttattg ccacagggt gggggaaga 
      ccgaagtcg ggggttccc agagcgata ttttgatga 
      aagtggatt tggagtatt tttcgaatc aaatttgcc 
      gccaaaatt acgccaacg ttgggagtg aatatctct 
      caagggggg ttgatgaca acagccaga tttgtgcag 
      gaagtcgca aggatggga aggacacgt gtcagggtg 
      attccgggg gtcccctgc aaatccgta ccgacgtcc 
      tgtacttag tcgggatca cgtcctttg gaaaaatct 
      gtggcatcc caaacagtc cgggggttt acacgagag 
      tctcgtatt acataccgt ggatcgaac gaattgttt 
      ggccccaat aatacagtt aaataaatt ataacctta 
      aaa
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