General information | |||
TC005276 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450917.1: 1917..21557 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC005276 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06673 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 3
abdomen musculature random partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
labium proximal swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
labium orientation irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
dorsal ridge row not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax tracheal opening number increased - Penetrance (percentage of animals/eggs on slide): more than 80%
Comment: |
prothorax tracheal opening ectopically present - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC005276 RA
MRKAENEMS NKMRKILVK DMNDVLVCT LCNGYLVDA TTLTECHHV FCRGCILRH FETCKTACP SCNLVYKKK NQVFFKADP QIQSIVYKV VPGLYSKEM QRREDFYRS TGVRASSSC SDDSVLERE REIIKDEDS MVSSYTGSK TQFFGPEDS ISLSLEYYQ VHLDKTPVE GSKSIKSSD KSVSVLNNN NSEAVSDKH KLNGTKNGE CKSGDKRYL QCPAAVSMK HLQKFIRMK FALSQNHKV DIIYEGEVL HSNFTLMDV AYTFKWKRL KPLRFFYRI FTPLKIQPI KIVNSGKQL QIVKNGEPI TPKPEPEVI SEEKERLLA NLQLQSKTK VQNDNNVPE KVAQDCVFD YEEQDKEEI KRFAEIRDR EWALQKKLD ESREKERDE DYHHFSKKR KKSKHSKND CVYKKRKLH AEITNEEEL KLKVKLTPH NGHKHKHHK SEMSSKEKL LQMRQVRHK HVTSEDKNV QTASTEPPT ADKKPEPEE INGEKKEET KEAKEPKKP IPDLAPKTL RSDFPAKTL NKEWGHRVP TVQIERNEQ TEQQAKKTF LKSYQAYAE KYQKTIEKT QSANRPAST LERKIANLQ QRCTIEAKP PEKPPQYPP GFTVSKIES GVPNNKEEK REEKRPSLE ITLINPPEV KPPKRPPPA TIPLDRIKK SLIFKTGIS IIPKDNIGA LDLSAKPPS KEGDPPNGF APPKDTKNL SNLQMLSKV ATEHPSLNK PKPRPQLPN LQTLKIPSP NQRIPPNLK LINKGQFRM MNPQIRNLR PNQNQSIRN IPNPSLVVR QQQNRINSM NPVPAPSNN VENKEKKEP ESKPEVSV
- >TC005276 RA
atgcgcaag gctgagaac gaaatgagc aacaaaatg cgaaaaatc ctcgtcaag gacatgaac gacgtgctc gtctgcacg ctatgtaac gggtatttg gtcgacgca accacactt accgaatgt caccatgtt ttctgtcgc ggctgcatt ttgcgccac tttgagacc tgcaaaacg gcatgtcct tcgtgcaat ctcgtctac aagaagaaa aaccaagtt ttcttcaaa gccgatccg caaattcag tcgattgtt tataaagtg gtgccaggg ttgtactcg aaggagatg caacgcagg gaggatttt tacaggagt accggagtt agggccagt agttcatgt agcgatgat agtgtcctt gaacgcgaa cgcgaaata ataaaggac gaggactca atggtgtcg agttatact gggagtaaa acacagttt ttcggcccc gaagactcg atttccctc tcgttggag tactaccaa gtccatttg gacaaaact ccggtggaa gggagcaaa tccatcaag tcgtcggac aagagtgtc agtgtttta aataataat aatagtgag gcggttagt gataaacat aaattgaac ggtacgaaa aatggtgag tgtaaaagt ggtgataag agatatctg cagtgtccg gcggctgtt tcgatgaaa catttacaa aagtttatt cggatgaag tttgctcta agccagaat cataaggtt gatattatt tatgagggg gaggttcta cactcgaat tttactcta atggacgtc gcttacacc ttcaaatgg aaaaggtta aaacctcta cgtttcttc taccgcatc ttcacccca ttgaaaatc cagccaatc aaaatcgtc aattcgggc aaacagctg caaatcgtc aaaaatggc gaaccgata acacccaag cccgagcca gaggtgata agtgaagaa aaggagcga cttttggcc aatcttcaa ttgcaaagc aagaccaaa gtacaaaac gacaataac gtacctgaa aaagtggca caagactgc gttttcgac tacgaggag caagacaag gaggagatc aagcggttc gcggagatt cgggaccgg gagtgggct ctccagaaa aagctggat gagagcagg gagaaggaa cgggatgag gactaccac catttcagc aagaaacgg aagaagagc aaacattcg aagaacgac tgcgtgtac aagaagcgg aaacttcat gccgaaatc acaaacgaa gaagagttg aagttgaag gtcaagttg acgccacac aatggccac aagcacaaa caccacaaa agtgagatg agtagcaag gaaaagttg ctacagatg agacaagtg agacacaaa cacgtcaca agtgaagat aagaacgtc caaacggca agcaccgaa cccccaact gctgacaaa aaaccagaa cctgaagaa atcaacgga gagaagaaa gaagaaacg aaggaagca aaagaaccg aaaaaaccg atacctgac ctcgccccc aagacactc cgtagcgat ttccccgcc aaaacccta aacaaagaa tggggacat agagtacca accgtacaa atcgaacga aatgaacag acggaacaa caagccaaa aaaaccttc ctcaagtcg taccaagcc tacgccgaa aagtaccaa aaaacaatc gagaagacc caaagtgcc aacagaccc gcgtcaacc cttgagcga aaaatcgcc aacttgcaa caaagatgc accatcgaa gccaaaccc cccgagaaa cccccccag tacccccct ggcttcacc gtgagcaaa atcgagagt ggggtcccc aacaacaag gaggaaaaa cgggaggag aaacgccca agtctcgaa atcacccta atcaacccc cctgaagtc aaacccccc aagcggccc cctcccgcc acgatcccc ctcgacagg attaagaaa tccctcatt ttcaaaact gggattagc attatcccc aaggacaac atcggggcg cttgactta tcggcgaag ccgcccagc aaggagggg gaccccccg aatgggttc gcgcccccc aaggacacc aagaacttg tccaatctc cagatgctg tcaaaagtg gcaacggaa cacccctcc cttaacaaa cccaagcca aggccacaa ctccccaac ctccaaacc cttaaaatc ccgtcgccg aatcagagg attccccca aaccttaaa ctgataaat aaagggcag tttcggatg atgaatccg caaatcagg aacttgagg ccgaatcag aatcagtcg attcggaat attccgaat ccgagtctt gtcgtgcgt cagcaacag aatcggatc aattcgatg aatccggtg ccagcgccg agcaataac gtggagaat aaggagaag aaagagccg gaaagtaaa ccggaagtt tcggtttga
- >TC005276 RA
tgttttcca cctgaaaac cagcgaatt ttcgcacgg gaaaaacgg tgttcggtt cggaaaaca cccggctat catctgtgc aaatttcgt gaaaattcg accaaggga aaaccccca aaaacgatg cccaacacc ggaatattt taacatgca gtacagtat ctgtgtatg taggcgatg cggcgttgc cggatcgac gccagtgcg acgttgcca cgttcggga cagattttt aacgccgag tgctccgga gcgatccgc tggtgacag ttgaatgcg caaggctga gaacgaaat gagcaacaa aatgcgaaa aatcctcgt caaggacat gaacgacgt gctcgtctg cacgctatg taacgggta tttggtcga cgcaaccac acttaccga atgtcacca tgttttctg tcgcggctg cattttgcg ccactttga gacctgcaa aacggcatg tccttcgtg caatctcgt ctacaagaa gaaaaacca agttttctt caaagccga tccgcaaat tcagtcgat tgtttataa agtggtgcc agggttgta ctcgaagga gatgcaacg cagggagga tttttacag gagtaccgg agttagggc cagtagttc atgtagcga tgatagtgt ccttgaacg cgaacgcga aataataaa ggacgagga ctcaatggt gtcgagtta tactgggag taaaacaca gtttttcgg ccccgaaga ctcgatttc cctctcgtt ggagtacta ccaagtcca tttggacaa aactccggt ggaagggag caaatccat caagtcgtc ggacaagag tgtcagtgt tttaaataa taataatag tgaggcggt tagtgataa acataaatt gaacggtac gaaaaatgg tgagtgtaa aagtggtga taagagata tctgcagtg tccggcggc tgtttcgat gaaacattt acaaaagtt tattcggat gaagtttgc tctaagcca gaatcataa ggttgatat tatttatga gggggaggt tctacactc gaattttac tctaatgga cgtcgctta caccttcaa atggaaaag gttaaaacc tctacgttt cttctaccg catcttcac cccattgaa aatccagcc aatcaaaat cgtcaattc gggcaaaca gctgcaaat cgtcaaaaa tggcgaacc gataacacc caagcccga gccagaggt gataagtga agaaaagga gcgactttt ggccaatct tcaattgca aagcaagac caaagtaca aaacgacaa taacgtacc tgaaaaagt ggcacaaga ctgcgtttt cgactacga ggagcaaga caaggagga gatcaagcg gttcgcgga gattcggga ccgggagtg ggctctcca gaaaaagct ggatgagag cagggagaa ggaacggga tgaggacta ccaccattt cagcaagaa acggaagaa gagcaaaca ttcgaagaa cgactgcgt gtacaagaa gcggaaact tcatgccga aatcacaaa cgaagaaga gttgaagtt gaaggtcaa gttgacgcc acacaatgg ccacaagca caaacacca caaaagtga gatgagtag caaggaaaa gttgctaca gatgagaca agtgagaca caaacacgt cacaagtga agataagaa cgtccaaac ggcaagcac cgaaccccc aactgctga caaaaaacc agaacctga agaaatcaa cggagagaa gaaagaaga aacgaagga agcaaaaga accgaaaaa accgatacc tgacctcgc ccccaagac actccgtag cgatttccc cgccaaaac cctaaacaa agaatgggg acatagagt accaaccgt acaaatcga acgaaatga acagacgga acaacaagc caaaaaaac cttcctcaa gtcgtacca agcctacgc cgaaaagta ccaaaaaac aatcgagaa gacccaaag tgccaacag acccgcgtc aacccttga gcgaaaaat cgccaactt gcaacaaag atgcaccat cgaagccaa accccccga gaaaccccc ccagtaccc ccctggctt caccgtgag caaaatcga gagtggggt ccccaacaa caaggagga aaaacggga ggagaaacg cccaagtct cgaaatcac cctaatcaa cccccctga agtcaaacc ccccaagcg gccccctcc cgccacgat ccccctcga caggattaa gaaatccct cattttcaa aactgggat tagcattat ccccaagga caacatcgg ggcgcttga cttatcggc gaagccgcc cagcaagga gggggaccc cccgaatgg gttcgcgcc ccccaagga caccaagaa cttgtccaa tctccagat gctgtcaaa agtggcaac ggaacaccc ctcccttaa caaacccaa gccaaggcc acaactccc caacctcca aacccttaa aatcccgtc gccgaatca gaggattcc cccaaacct taaactgat aaataaagg gcagtttcg gatgatgaa tccgcaaat caggaactt gaggccgaa tcagaatca gtcgattcg gaatattcc gaatccgag tcttgtcgt gcgtcagca acagaatcg gatcaattc gatgaatcc ggtgccagc gccgagcaa taacgtgga gaataagga gaagaaaga gccggaaag taaaccgga agtttcggt ttgaaggcc ggatgtact gaaggtccc agtgagtta cgttatgtt cctaattta agacttttg ttatggttt tgttgtgtt gtgattgtt tcatgttgc actttttta attcggtca ctttgtttc gatgttatc gatgtatga ggtcgctca gactgtact tattcatta ttatataaa tatgtatat cgtagctat gtttaagta ggttttagg agtatgtgt atgtataga acactctgt atataatta aaaattgat taaatga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
