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Gene information
General information
TC005276 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450917.1: 1917..21557
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005276
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06673

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature specific subset ventral partiallyrounded - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen musculature random partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labium proximal swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
    labium orientation irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    dorsal ridge row not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax tracheal opening number increased - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment:
    prothorax potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%

    prothorax tracheal opening ectopically present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005276 RA
      MRKAENEMS NKMRKILVK DMNDVLVCT LCNGYLVDA 
      TTLTECHHV FCRGCILRH FETCKTACP SCNLVYKKK 
      NQVFFKADP QIQSIVYKV VPGLYSKEM QRREDFYRS 
      TGVRASSSC SDDSVLERE REIIKDEDS MVSSYTGSK 
      TQFFGPEDS ISLSLEYYQ VHLDKTPVE GSKSIKSSD 
      KSVSVLNNN NSEAVSDKH KLNGTKNGE CKSGDKRYL 
      QCPAAVSMK HLQKFIRMK FALSQNHKV DIIYEGEVL 
      HSNFTLMDV AYTFKWKRL KPLRFFYRI FTPLKIQPI 
      KIVNSGKQL QIVKNGEPI TPKPEPEVI SEEKERLLA 
      NLQLQSKTK VQNDNNVPE KVAQDCVFD YEEQDKEEI 
      KRFAEIRDR EWALQKKLD ESREKERDE DYHHFSKKR 
      KKSKHSKND CVYKKRKLH AEITNEEEL KLKVKLTPH 
      NGHKHKHHK SEMSSKEKL LQMRQVRHK HVTSEDKNV 
      QTASTEPPT ADKKPEPEE INGEKKEET KEAKEPKKP 
      IPDLAPKTL RSDFPAKTL NKEWGHRVP TVQIERNEQ 
      TEQQAKKTF LKSYQAYAE KYQKTIEKT QSANRPAST 
      LERKIANLQ QRCTIEAKP PEKPPQYPP GFTVSKIES 
      GVPNNKEEK REEKRPSLE ITLINPPEV KPPKRPPPA 
      TIPLDRIKK SLIFKTGIS IIPKDNIGA LDLSAKPPS 
      KEGDPPNGF APPKDTKNL SNLQMLSKV ATEHPSLNK 
      PKPRPQLPN LQTLKIPSP NQRIPPNLK LINKGQFRM 
      MNPQIRNLR PNQNQSIRN IPNPSLVVR QQQNRINSM 
      NPVPAPSNN VENKEKKEP ESKPEVSV
    CDS sequence
    • >TC005276 RA
      atgcgcaag gctgagaac gaaatgagc aacaaaatg 
      cgaaaaatc ctcgtcaag gacatgaac gacgtgctc 
      gtctgcacg ctatgtaac gggtatttg gtcgacgca 
      accacactt accgaatgt caccatgtt ttctgtcgc 
      ggctgcatt ttgcgccac tttgagacc tgcaaaacg 
      gcatgtcct tcgtgcaat ctcgtctac aagaagaaa 
      aaccaagtt ttcttcaaa gccgatccg caaattcag 
      tcgattgtt tataaagtg gtgccaggg ttgtactcg 
      aaggagatg caacgcagg gaggatttt tacaggagt 
      accggagtt agggccagt agttcatgt agcgatgat 
      agtgtcctt gaacgcgaa cgcgaaata ataaaggac 
      gaggactca atggtgtcg agttatact gggagtaaa 
      acacagttt ttcggcccc gaagactcg atttccctc 
      tcgttggag tactaccaa gtccatttg gacaaaact 
      ccggtggaa gggagcaaa tccatcaag tcgtcggac 
      aagagtgtc agtgtttta aataataat aatagtgag 
      gcggttagt gataaacat aaattgaac ggtacgaaa 
      aatggtgag tgtaaaagt ggtgataag agatatctg 
      cagtgtccg gcggctgtt tcgatgaaa catttacaa 
      aagtttatt cggatgaag tttgctcta agccagaat 
      cataaggtt gatattatt tatgagggg gaggttcta 
      cactcgaat tttactcta atggacgtc gcttacacc 
      ttcaaatgg aaaaggtta aaacctcta cgtttcttc 
      taccgcatc ttcacccca ttgaaaatc cagccaatc 
      aaaatcgtc aattcgggc aaacagctg caaatcgtc 
      aaaaatggc gaaccgata acacccaag cccgagcca 
      gaggtgata agtgaagaa aaggagcga cttttggcc 
      aatcttcaa ttgcaaagc aagaccaaa gtacaaaac 
      gacaataac gtacctgaa aaagtggca caagactgc 
      gttttcgac tacgaggag caagacaag gaggagatc 
      aagcggttc gcggagatt cgggaccgg gagtgggct 
      ctccagaaa aagctggat gagagcagg gagaaggaa 
      cgggatgag gactaccac catttcagc aagaaacgg 
      aagaagagc aaacattcg aagaacgac tgcgtgtac 
      aagaagcgg aaacttcat gccgaaatc acaaacgaa 
      gaagagttg aagttgaag gtcaagttg acgccacac 
      aatggccac aagcacaaa caccacaaa agtgagatg 
      agtagcaag gaaaagttg ctacagatg agacaagtg 
      agacacaaa cacgtcaca agtgaagat aagaacgtc 
      caaacggca agcaccgaa cccccaact gctgacaaa 
      aaaccagaa cctgaagaa atcaacgga gagaagaaa 
      gaagaaacg aaggaagca aaagaaccg aaaaaaccg 
      atacctgac ctcgccccc aagacactc cgtagcgat 
      ttccccgcc aaaacccta aacaaagaa tggggacat 
      agagtacca accgtacaa atcgaacga aatgaacag 
      acggaacaa caagccaaa aaaaccttc ctcaagtcg 
      taccaagcc tacgccgaa aagtaccaa aaaacaatc 
      gagaagacc caaagtgcc aacagaccc gcgtcaacc 
      cttgagcga aaaatcgcc aacttgcaa caaagatgc 
      accatcgaa gccaaaccc cccgagaaa cccccccag 
      tacccccct ggcttcacc gtgagcaaa atcgagagt 
      ggggtcccc aacaacaag gaggaaaaa cgggaggag 
      aaacgccca agtctcgaa atcacccta atcaacccc 
      cctgaagtc aaacccccc aagcggccc cctcccgcc 
      acgatcccc ctcgacagg attaagaaa tccctcatt 
      ttcaaaact gggattagc attatcccc aaggacaac 
      atcggggcg cttgactta tcggcgaag ccgcccagc 
      aaggagggg gaccccccg aatgggttc gcgcccccc 
      aaggacacc aagaacttg tccaatctc cagatgctg 
      tcaaaagtg gcaacggaa cacccctcc cttaacaaa 
      cccaagcca aggccacaa ctccccaac ctccaaacc 
      cttaaaatc ccgtcgccg aatcagagg attccccca 
      aaccttaaa ctgataaat aaagggcag tttcggatg 
      atgaatccg caaatcagg aacttgagg ccgaatcag 
      aatcagtcg attcggaat attccgaat ccgagtctt 
      gtcgtgcgt cagcaacag aatcggatc aattcgatg 
      aatccggtg ccagcgccg agcaataac gtggagaat 
      aaggagaag aaagagccg gaaagtaaa ccggaagtt 
      tcggtttga
    mRNA sequence
    • >TC005276 RA
      tgttttcca cctgaaaac cagcgaatt ttcgcacgg 
      gaaaaacgg tgttcggtt cggaaaaca cccggctat 
      catctgtgc aaatttcgt gaaaattcg accaaggga 
      aaaccccca aaaacgatg cccaacacc ggaatattt 
      taacatgca gtacagtat ctgtgtatg taggcgatg 
      cggcgttgc cggatcgac gccagtgcg acgttgcca 
      cgttcggga cagattttt aacgccgag tgctccgga 
      gcgatccgc tggtgacag ttgaatgcg caaggctga 
      gaacgaaat gagcaacaa aatgcgaaa aatcctcgt 
      caaggacat gaacgacgt gctcgtctg cacgctatg 
      taacgggta tttggtcga cgcaaccac acttaccga 
      atgtcacca tgttttctg tcgcggctg cattttgcg 
      ccactttga gacctgcaa aacggcatg tccttcgtg 
      caatctcgt ctacaagaa gaaaaacca agttttctt 
      caaagccga tccgcaaat tcagtcgat tgtttataa 
      agtggtgcc agggttgta ctcgaagga gatgcaacg 
      cagggagga tttttacag gagtaccgg agttagggc 
      cagtagttc atgtagcga tgatagtgt ccttgaacg 
      cgaacgcga aataataaa ggacgagga ctcaatggt 
      gtcgagtta tactgggag taaaacaca gtttttcgg 
      ccccgaaga ctcgatttc cctctcgtt ggagtacta 
      ccaagtcca tttggacaa aactccggt ggaagggag 
      caaatccat caagtcgtc ggacaagag tgtcagtgt 
      tttaaataa taataatag tgaggcggt tagtgataa 
      acataaatt gaacggtac gaaaaatgg tgagtgtaa 
      aagtggtga taagagata tctgcagtg tccggcggc 
      tgtttcgat gaaacattt acaaaagtt tattcggat 
      gaagtttgc tctaagcca gaatcataa ggttgatat 
      tatttatga gggggaggt tctacactc gaattttac 
      tctaatgga cgtcgctta caccttcaa atggaaaag 
      gttaaaacc tctacgttt cttctaccg catcttcac 
      cccattgaa aatccagcc aatcaaaat cgtcaattc 
      gggcaaaca gctgcaaat cgtcaaaaa tggcgaacc 
      gataacacc caagcccga gccagaggt gataagtga 
      agaaaagga gcgactttt ggccaatct tcaattgca 
      aagcaagac caaagtaca aaacgacaa taacgtacc 
      tgaaaaagt ggcacaaga ctgcgtttt cgactacga 
      ggagcaaga caaggagga gatcaagcg gttcgcgga 
      gattcggga ccgggagtg ggctctcca gaaaaagct 
      ggatgagag cagggagaa ggaacggga tgaggacta 
      ccaccattt cagcaagaa acggaagaa gagcaaaca 
      ttcgaagaa cgactgcgt gtacaagaa gcggaaact 
      tcatgccga aatcacaaa cgaagaaga gttgaagtt 
      gaaggtcaa gttgacgcc acacaatgg ccacaagca 
      caaacacca caaaagtga gatgagtag caaggaaaa 
      gttgctaca gatgagaca agtgagaca caaacacgt 
      cacaagtga agataagaa cgtccaaac ggcaagcac 
      cgaaccccc aactgctga caaaaaacc agaacctga 
      agaaatcaa cggagagaa gaaagaaga aacgaagga 
      agcaaaaga accgaaaaa accgatacc tgacctcgc 
      ccccaagac actccgtag cgatttccc cgccaaaac 
      cctaaacaa agaatgggg acatagagt accaaccgt 
      acaaatcga acgaaatga acagacgga acaacaagc 
      caaaaaaac cttcctcaa gtcgtacca agcctacgc 
      cgaaaagta ccaaaaaac aatcgagaa gacccaaag 
      tgccaacag acccgcgtc aacccttga gcgaaaaat 
      cgccaactt gcaacaaag atgcaccat cgaagccaa 
      accccccga gaaaccccc ccagtaccc ccctggctt 
      caccgtgag caaaatcga gagtggggt ccccaacaa 
      caaggagga aaaacggga ggagaaacg cccaagtct 
      cgaaatcac cctaatcaa cccccctga agtcaaacc 
      ccccaagcg gccccctcc cgccacgat ccccctcga 
      caggattaa gaaatccct cattttcaa aactgggat 
      tagcattat ccccaagga caacatcgg ggcgcttga 
      cttatcggc gaagccgcc cagcaagga gggggaccc 
      cccgaatgg gttcgcgcc ccccaagga caccaagaa 
      cttgtccaa tctccagat gctgtcaaa agtggcaac 
      ggaacaccc ctcccttaa caaacccaa gccaaggcc 
      acaactccc caacctcca aacccttaa aatcccgtc 
      gccgaatca gaggattcc cccaaacct taaactgat 
      aaataaagg gcagtttcg gatgatgaa tccgcaaat 
      caggaactt gaggccgaa tcagaatca gtcgattcg 
      gaatattcc gaatccgag tcttgtcgt gcgtcagca 
      acagaatcg gatcaattc gatgaatcc ggtgccagc 
      gccgagcaa taacgtgga gaataagga gaagaaaga 
      gccggaaag taaaccgga agtttcggt ttgaaggcc 
      ggatgtact gaaggtccc agtgagtta cgttatgtt 
      cctaattta agacttttg ttatggttt tgttgtgtt 
      gtgattgtt tcatgttgc actttttta attcggtca 
      ctttgtttc gatgttatc gatgtatga ggtcgctca 
      gactgtact tattcatta ttatataaa tatgtatat 
      cgtagctat gtttaagta ggttttagg agtatgtgt 
      atgtataga acactctgt atataatta aaaattgat 
      taaatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY