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Gene information
General information
TC000544 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9935100..9940737
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000544
    iBeetle screen iB_06668

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000544 RA
      MYGMLLESV QHFVQLEYG EEIWQKALH LSECKYTVF 
      STHQVYPDH IMGALAAAL AEITAQSTD AFMNFFGKC 
      FVRFFSNYG YDLTIKATG RNFTDFLEN VDNIHIQFT 
      LSYPKMKSP SMYVTEVDE NGCVLVYRS GRQGFNHYL 
      MGQLHQIAE DIFNLKLET TVLNQENKT IGGRNITIV 
      NFRLNFDNS QYIAGKQAE AAAHLEISQ LAPFSCSLL 
      LELFPCAIL FNPLLEIIG CGEKLVHLS GGKSKLLRK 
      PVTKFFRLR RPKGISFTW KNIINLKSV MFELELLRS 
      ELVQKADIG DTLDDKTLE IEIDGKSIS PYALRRDSQ 
      PGLKNILLK GQMRFLDDI NAAIYLCSP VVNDINELP 
      DQGLYLNDL NQHGLGKEM VLAGWQHNS KLGLMFDKA 
      EQRATELEN NHSLLDTWK RRGDDLLYS MIPKTVADR 
      LRNGKSPLS TCETFDSVT IMFCELVGF NSTTVQDAM 
      ELVSTMNAV FSCFDSLMD RFNVYKVET VGQVYMAVS 
      GAPERTSSH AQNVCDVSL CMMHQVQKL QIPSGSGTR 
      VVEVRIGVH SGPVVAGVV GIKVPRYCF FGDTVNTAS 
      RMQSTSSPG MVHISAQTK DKLPEERYL IEDRGLVKL 
      KGKGEVQTF WIFEKEPIA VRFRYQLSI KQSIELVLA 
      VASKPINMY GMLLESVQY FIQLEYGEE IWQRVLEQA 
      NCKYISFET HKVYSDNVM ASLAAACAE VTTASYDSF 
      MNFFGRCFV RYFSNLGYD VTVKATGRF FTDFLQSVD 
      NIHSQFCFT YPKMKSPSI YLTDIDSGG CVLVYRSSR 
      QGFTQYVMG QLQQIAQDF YNLKLKVRV LEKASSAAG 
      TKSTVIVTY RLDFDNKPY MQYKAKRTS FNEFIRLSP 
      FPCSLLLEL FPFGLIINP NMNIMGLGE KLSEIWKGK 
      EDFLNKPVG FYFKLRRPK GITFLWKNT RNLESVMFE 
      LECNRGANN FTKTESKTD ANRAASPEL KNLLLKGQM 
      KFVKDINAI IFLCSPIIN DLDELPEQG LYLNDLNQH 
      GLSKEMVLA GWQHNSKLE IMFDKEAQR SDELEKSYE 
      LLDTWKRRG DDLLYSMIP QTVADRLRT GNSPLSTCE 
      SFEAVTILF CELVGLSSE TVKDAMQVV STMNTVFSC 
      FDSLMDKFG VYKVETVGQ IYMAVSGAP ERTPKHAEN 
      IASLSFEML KQTDEIKSP DGAKVNIRI GVHSGPAVA 
      GVVGIKVPR YCFFGDTVN TASRMQSTS SPGMINISA 
      DTKNLLPPK QYVIKNRGK VKVKGKGEL EAFWLLN
    CDS sequence
    • >TC000544 RA
      atgtacgga atgttactc gaaagtgtg cagcatttt 
      gtccagtta gaatacggc gaagaaatt tggcagaaa 
      gctctgcat ttgtcggaa tgcaagtac acggtcttc 
      agcacccat caggtgtac cctgaccac atcatgggc 
      gctctggcg gccgctttg gccgaaatc accgcccag 
      tccacggac gctttcatg aattttttc ggcaagtgc 
      ttcgtacga tttttcagc aattacggt tatgacctt 
      acaattaaa gcaacaggt cgcaatttc actgatttc 
      ttggaaaat gtcgataat atccacatt cagttcaca 
      ctttcttac ccgaagatg aaaagcccg tccatgtat 
      gtaacggaa gttgatgaa aatggatgt gtattggtt 
      tatcggagc ggaagacag ggattcaat cattattta 
      atgggtcaa ttgcatcag attgcggaa gatattttt 
      aatctcaag ttggaaaca actgtcctt aaccaggaa 
      aataaaacg attggtgga cgaaacata acaatcgtc 
      aatttcagg ctcaacttt gacaactca caatacata 
      gccggaaag caggccgaa gccgctgcg cacttagag 
      atcagccaa ctggccccc ttttcctgc agcctcctc 
      ctggagctt ttcccttgc gcgatcctc ttcaacccg 
      ctgctggag atcatcggt tgtggcgaa aagctcgtg 
      cacttgtcc ggaggcaag agcaagctt ttgcgaaag 
      cccgtgaca aagtttttc cggttgagg cgccccaaa 
      gggatcagt ttcacgtgg aaaaatatt attaacttg 
      aaatcggtc atgttcgag ctggagctg ctccggtcc 
      gagctggtg caaaaggcc gacattggc gacaccctc 
      gatgataag actctagag attgaaatc gacgggaaa 
      agcatctcg ccgtatgct ttgaggcga gacagccag 
      cccggcttg aaaaacatc cttttgaaa ggtcagatg 
      cggtttctt gacgacata aatgcggct atttatctc 
      tgcagccct gtagtgaat gatattaac gagctgcct 
      gatcaaggg ctttatctc aacgacctg aatcaacac 
      gggctgggg aaggagatg gtgctagcg gggtggcaa 
      cacaactcc aaattgggg ctgatgttc gataaagcg 
      gaacagagg gccactgaa ctcgagaat aaccacagc 
      ttgttggac acgtggaag aggagagga gacgatctt 
      ttgtactcc atgataccc aaaacggtc gccgatcgg 
      ctcagaaat ggcaaatcg cccttaagc acgtgtgag 
      acattcgat tctgtcacg ataatgttt tgcgagctg 
      gtgggtttc aactccacg acggtgcaa gacgccatg 
      gagttggtc tcaaccatg aacgcagtt ttctcctgt 
      ttcgactct ctcatggac agattcaac gtctataag 
      gttgaaact gtgggtcaa gtctacatg gcggtgagc 
      ggagccccc gaaagaacc tcctcccac gcccaaaac 
      gtctgcgac gtctccctc tgtatgatg caccaggtc 
      cagaaactc caaatcccg tcaggaagt ggaacacga 
      gttgtggaa gttcgcatt ggagtgcat tcaggtcct 
      gtcgttgct ggagttgtg ggtatcaaa gtcccaaga 
      tattgcttt ttcggtgac acggttaat acagcgtca 
      cgaatgcag tcgacaagt tcgccggga atggtgcac 
      atttccgca cagactaag gacaaactc cctgaagag 
      cgctacctt attgaggat agaggactc gtgaaactg 
      aagggaaaa ggagaagtc cagacgttc tggatattt 
      gagaaggaa ccgatcgct gtccggttc agataccag 
      ctctcgatt aaacagagc atagagcta gtgcttgcg 
      gttgcctcc aaaccgatc aacatgtac ggtatgttg 
      ttggaaagt gttcagtat tttattcag ctagaatat 
      ggcgaagaa atttggcaa cgagttttg gaacaagcc 
      aattgcaaa tacatttcg tttgaaaca cataaagtg 
      tactcggac aacgtgatg gcatctctg gctgcagcc 
      tgtgccgaa gtcacgacg gcttcgtac gatagcttc 
      atgaatttt ttcgggcgc tgtttcgtg cgatatttc 
      tcgaatttg ggttacgat gtgactgtg aaagcaacc 
      gggcgattc ttcacggat tttctccaa agcgttgac 
      aatatccac tcacagttt tgcttcact tatccaaaa 
      atgaaaagc ccctcaatt tatttaacc gacattgat 
      tcaggtggt tgcgttttg gtttacaga agctcaaga 
      caagggttc actcaatac gtcatgggt caattgcaa 
      caaatcgcg caagatttt tataatctc aaactaaaa 
      gtccgcgtt ttggaaaaa gccagctct gctgccggc 
      accaaaagc acggtaatt gtgacttac agactcgat 
      ttcgataac aagccatat atgcaatac aaagcgaaa 
      cgaacgtcg tttaacgaa ttcattcga ttatcgcca 
      tttccgtgc tccctcctg ttggagtta ttcccgttc 
      gggttaata ataaacccg aatatgaat ataatggga 
      ctgggcgag aaattatcc gaaatatgg aaaggcaag 
      gaagatttt ctcaacaaa cctgtcggc ttttacttc 
      aagctgagg cgtcctaaa ggaatcaca tttttatgg 
      aaaaataca cgcaatttg gagtcagtc atgtttgaa 
      ctggagtgc aaccgcggc gcgaataat ttcaccaaa 
      actgagagc aaaactgac gcaaatcgg gccgcctcc 
      cccgaactc aaaaacctg ctcctgaag ggtcaaatg 
      aaattcgtt aaagacatc aacgccatt attttcctc 
      tgcagtccg ataattaat gatctggat gagttaccg 
      gagcaaggt ttgtatctc aacgatttg aaccaacac 
      ggcttgagc aaggagatg gtgttggcc ggctggcaa 
      cacaactcc aaactcgaa attatgttc gataaagag 
      gcgcaaagg tcggatgag ttggagaaa agttacgaa 
      cttttagac acttggaaa cgcagaggt gatgatctt 
      ttgtactcc atgattcct caaaccgtc gctgatcga 
      ttacgcacc ggaaactct cctttgagc acttgcgaa 
      tcgtttgaa gccgtcacg attcttttt tgcgagctg 
      gtcgggctg tcctctgaa acggtgaaa gacgccatg 
      caagtggtc tcaacaatg aacacggtt ttttcgtgt 
      tttgattca ctcatggac aaatttggc gtttataaa 
      gtcgaaact gttggtcag atctacatg gccgttagt 
      ggggcccct gaaaggacc ccaaaacac gccgaaaac 
      atcgcatcc ctatcgttt gaaatgcta aaacaaacc 
      gacgagatt aagagtcca gatggtgca aaagtcaat 
      atcaggatc ggagttcac tcaggccct gctgttgcc 
      ggagttgtg gggattaaa gtcccccgt tactgtttt 
      ttcggagat acggttaac acagcctcc agaatgcag 
      tccacaagc tcacccgga atgattaac atttcagcc 
      gacactaaa aacctgctg ccgccgaaa caatacgtt 
      atcaaaaat cgcggaaaa gtcaaagtt aaggggaag 
      ggtgagtta gaagcgttt tggcttttg aattaa
    mRNA sequence
    • >TC000544 RA
      gcacgtatt aatttccct cttcataaa ataaatgca 
      aaatatcgg tgcaaaatg tacggaatg ttactcgaa 
      agtgtgcag cattttgtc cagttagaa tacggcgaa 
      gaaatttgg cagaaagct ctgcatttg tcggaatgc 
      aagtacacg gtcttcagc acccatcag gtgtaccct 
      gaccacatc atgggcgct ctggcggcc gctttggcc 
      gaaatcacc gcccagtcc acggacgct ttcatgaat 
      tttttcggc aagtgcttc gtacgattt ttcagcaat 
      tacggttat gaccttaca attaaagca acaggtcgc 
      aatttcact gatttcttg gaaaatgtc gataatatc 
      cacattcag ttcacactt tcttacccg aagatgaaa 
      agcccgtcc atgtatgta acggaagtt gatgaaaat 
      ggatgtgta ttggtttat cggagcgga agacaggga 
      ttcaatcat tatttaatg ggtcaattg catcagatt 
      gcggaagat atttttaat ctcaagttg gaaacaact 
      gtccttaac caggaaaat aaaacgatt ggtggacga 
      aacataaca atcgtcaat ttcaggctc aactttgac 
      aactcacaa tacatagcc ggaaagcag gccgaagcc 
      gctgcgcac ttagagatc agccaactg gcccccttt 
      tcctgcagc ctcctcctg gagcttttc ccttgcgcg 
      atcctcttc aacccgctg ctggagatc atcggttgt 
      ggcgaaaag ctcgtgcac ttgtccgga ggcaagagc 
      aagcttttg cgaaagccc gtgacaaag tttttccgg 
      ttgaggcgc cccaaaggg atcagtttc acgtggaaa 
      aatattatt aacttgaaa tcggtcatg ttcgagctg 
      gagctgctc cggtccgag ctggtgcaa aaggccgac 
      attggcgac accctcgat gataagact ctagagatt 
      gaaatcgac gggaaaagc atctcgccg tatgctttg 
      aggcgagac agccagccc ggcttgaaa aacatcctt 
      ttgaaaggt cagatgcgg tttcttgac gacataaat 
      gcggctatt tatctctgc agccctgta gtgaatgat 
      attaacgag ctgcctgat caagggctt tatctcaac 
      gacctgaat caacacggg ctggggaag gagatggtg 
      ctagcgggg tggcaacac aactccaaa ttggggctg 
      atgttcgat aaagcggaa cagagggcc actgaactc 
      gagaataac cacagcttg ttggacacg tggaagagg 
      agaggagac gatcttttg tactccatg atacccaaa 
      acggtcgcc gatcggctc agaaatggc aaatcgccc 
      ttaagcacg tgtgagaca ttcgattct gtcacgata 
      atgttttgc gagctggtg ggtttcaac tccacgacg 
      gtgcaagac gccatggag ttggtctca accatgaac 
      gcagttttc tcctgtttc gactctctc atggacaga 
      ttcaacgtc tataaggtt gaaactgtg ggtcaagtc 
      tacatggcg gtgagcgga gcccccgaa agaacctcc 
      tcccacgcc caaaacgtc tgcgacgtc tccctctgt 
      atgatgcac caggtccag aaactccaa atcccgtca 
      ggaagtgga acacgagtt gtggaagtt cgcattgga 
      gtgcattca ggtcctgtc gttgctgga gttgtgggt 
      atcaaagtc ccaagatat tgctttttc ggtgacacg 
      gttaataca gcgtcacga atgcagtcg acaagttcg 
      ccgggaatg gtgcacatt tccgcacag actaaggac 
      aaactccct gaagagcgc taccttatt gaggataga 
      ggactcgtg aaactgaag ggaaaagga gaagtccag 
      acgttctgg atatttgag aaggaaccg atcgctgtc 
      cggttcaga taccagctc tcgattaaa cagagcata 
      gagctagtg cttgcggtt gcctccaaa ccgatcaac 
      atgtacggt atgttgttg gaaagtgtt cagtatttt 
      attcagcta gaatatggc gaagaaatt tggcaacga 
      gttttggaa caagccaat tgcaaatac atttcgttt 
      gaaacacat aaagtgtac tcggacaac gtgatggca 
      tctctggct gcagcctgt gccgaagtc acgacggct 
      tcgtacgat agcttcatg aattttttc gggcgctgt 
      ttcgtgcga tatttctcg aatttgggt tacgatgtg 
      actgtgaaa gcaaccggg cgattcttc acggatttt 
      ctccaaagc gttgacaat atccactca cagttttgc 
      ttcacttat ccaaaaatg aaaagcccc tcaatttat 
      ttaaccgac attgattca ggtggttgc gttttggtt 
      tacagaagc tcaagacaa gggttcact caatacgtc 
      atgggtcaa ttgcaacaa atcgcgcaa gatttttat 
      aatctcaaa ctaaaagtc cgcgttttg gaaaaagcc 
      agctctgct gccggcacc aaaagcacg gtaattgtg 
      acttacaga ctcgatttc gataacaag ccatatatg 
      caatacaaa gcgaaacga acgtcgttt aacgaattc 
      attcgatta tcgccattt ccgtgctcc ctcctgttg 
      gagttattc ccgttcggg ttaataata aacccgaat 
      atgaatata atgggactg ggcgagaaa ttatccgaa 
      atatggaaa ggcaaggaa gattttctc aacaaacct 
      gtcggcttt tacttcaag ctgaggcgt cctaaagga 
      atcacattt ttatggaaa aatacacgc aatttggag 
      tcagtcatg tttgaactg gagtgcaac cgcggcgcg 
      aataatttc accaaaact gagagcaaa actgacgca 
      aatcgggcc gcctccccc gaactcaaa aacctgctc 
      ctgaagggt caaatgaaa ttcgttaaa gacatcaac 
      gccattatt ttcctctgc agtccgata attaatgat 
      ctggatgag ttaccggag caaggtttg tatctcaac 
      gatttgaac caacacggc ttgagcaag gagatggtg 
      ttggccggc tggcaacac aactccaaa ctcgaaatt 
      atgttcgat aaagaggcg caaaggtcg gatgagttg 
      gagaaaagt tacgaactt ttagacact tggaaacgc 
      agaggtgat gatcttttg tactccatg attcctcaa 
      accgtcgct gatcgatta cgcaccgga aactctcct 
      ttgagcact tgcgaatcg tttgaagcc gtcacgatt 
      cttttttgc gagctggtc gggctgtcc tctgaaacg 
      gtgaaagac gccatgcaa gtggtctca acaatgaac 
      acggttttt tcgtgtttt gattcactc atggacaaa 
      tttggcgtt tataaagtc gaaactgtt ggtcagatc 
      tacatggcc gttagtggg gcccctgaa aggacccca 
      aaacacgcc gaaaacatc gcatcccta tcgtttgaa 
      atgctaaaa caaaccgac gagattaag agtccagat 
      ggtgcaaaa gtcaatatc aggatcgga gttcactca 
      ggccctgct gttgccgga gttgtgggg attaaagtc 
      ccccgttac tgttttttc ggagatacg gttaacaca 
      gcctccaga atgcagtcc acaagctca cccggaatg 
      attaacatt tcagccgac actaaaaac ctgctgccg 
      ccgaaacaa tacgttatc aaaaatcgc ggaaaagtc 
      aaagttaag gggaagggt gagttagaa gcgttttgg 
      cttttgaat taatttatt agaaatatg tacaagtaa 
      taaatagtc tagtcctcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY