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Gene information
General information
TC031673
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002289
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 60449..65231
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031673
    iBeetle screen iB_06664

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031673 RA
      MPLLKFIQA TKNPSRRLK RRRKDREVT 
      LSKTREWGQ ALDSEHLSE TTEGEMEVS 
      FVSDRIKGF VKYDLKYFI PFFTRRFTQ 
      QELKDCKSQ MTDLTNQWY QAIRISPDQ 
      SDEENVTIT PRSSISSII H
    CDS sequence
    • >TC031673 RA
      atgcctttg ttaaagttc atacaagca acgaaaaat 
      ccgtccagg cgtcttaaa agacgccga aaagataga 
      gaagttact ttaagtaaa actagagaa tggggccaa 
      gctctagac tcagagcac ttgtctgaa actactgaa 
      ggagaaatg gaagtgtcg ttcgtctca gatcgaata 
      aagggtttt gttaaatat gacttgaag tatttcatc 
      ccatttttt actaggaga tttacacaa caggaactg 
      aaagattgc aaaagccaa atgacggat ttaacaaat 
      cagtggtac caggcaata aggatatct cctgaccag 
      tctgatgaa gaaaatgta acgattact cctcggtca 
      agtatttca agcattatt cattga
    mRNA sequence
    • >TC031673 RA
      cgaacaaaa atgtttgaa ggacgttct ctaaccttg 
      tcgaaatgt cagtgaaaa ttacaatta cgaatgagt 
      cactagaaa aaattaaag tgaatctgt gacaaacga 
      ttacaaaaa gtgttggca cttaagtta tttacatct 
      gatatcatg tgaggagac agcttagca acccaaatt 
      cctggtcgt aaagacatg caagttctt aaaattgtg 
      tatgtttta ttgctgagc caatgtgtc tctttgcaa 
      gactcacca aatgtgacc ctcatcgat aattcaaca 
      gtggtaaaa tccaacaac acacatgga attctcaca 
      tcaactacc cattcaccg cttgcaact tcatcatca 
      agcaaacca acaactgct ggacataac actgcgcaa 
      agtaacccc ccttccacc cctgttaaa aatgtcacg 
      acttcaagt cactctctc aaaaacaca acttctaat 
      gcaacatta tctgatccg atacttcct gacaaagga 
      gcagctgaa gatgaacat aacagttca atggctata 
      ttttttctt ctgtgtgtt ttagcactg ggcatttta 
      cttattcat tttatgctt cagacaggt tttcagtat 
      gttcctgag agcatagtt atagttttc cttggggca 
      ttaattggt ctagcaatg aatacttta tctaaccga 
      aatattagt aattggcgg aaagaggaa gccttttca 
      cctacggca tttttctta gttttactg ccaccaatt 
      atttttgaa tcaggttac aatttacat aaggggaat 
      ttctttcaa aatataggt tcgattttg gtctttgca 
      atattcgga acgacaatt tcggctttt ataattggt 
      tttgggata tatttcttg ggtttagcg gaagtcgtt 
      tataagctg agttttgtc gagtcattc gcttttggt 
      tccttaatt tcagctgtt gatcctgtg gcaactgtc 
      gctatattc cacgcactt aatgttgat cctgtactt 
      aatatgtta gtgtttggg gaaagtatt ttaaacgat 
      gcgattgct attgtttta acaacagca gcattagag 
      tcaaataat ccggtaatg tcaacaggg gaagcaatt 
      gtgatgggt atacagcgg ttctgtcta atgttcttc 
      gcttccgct ggtattggg gtaattttt gctcttgtg 
      agtgcccta tttctaaaa catgtcgat cttagaaag 
      aacccttcg ttggaattt gctatgatg ttagtgttt 
      acttatgca ccttatgta ttagcagaa ggaattcat 
      ctcagtggt attatggcc atactgttc tgtggcatt 
      gtaatgtca cattacacc cactttaat ctatccaca 
      gtaacgcaa attacgatg caacagacc ctcagaacc 
      ttagctttc atcgccgaa acttgtgtg tttgcatat 
      ttagggttg gctctgttt agttttaaa catcgagtg 
      gagcccgct ctgattact tggggcata attttgtgt 
      ctcattggc cgcgcttgt aatattttt ccactggcg 
      attctagtt aatcgattt cgtgaacat caaattacc 
      aaaaaaatg atgtttata atgtggttt agtggtctt 
      cgtggtgcc atttcttac gcattatcg cttcattta 
      aactttagc gatgaaacg cggcatgtt attattacc 
      acaacattg attattgtt ttgtttaca acgctattt 
      ttggggggc tctactatg cctttgtta aagttcata 
      caagcaacg aaaaatccg tccaggcgt cttaaaaga 
      cgccgaaaa gatagagaa gttacttta agtaaaact 
      agagaatgg ggccaagct ctagactca gagcacttg 
      tctgaaact actgaagga gaaatggaa gtgtcgttc 
      gtctcagat cgaataaag ggttttgtt aaatatgac 
      ttgaagtat ttcatccca ttttttact aggagattt 
      acacaacag gaactgaaa gattgcaaa agccaaatg 
      acggattta acaaatcag tggtaccag gcaataagg 
      atatctcct gaccagtct gatgaagaa aatgtaacg 
      attactcct cggtcaagt atttcaagc attattcat 
      tgagtacta taaaaagat atgaaaact gtgctgtat 
      tataactta taactaaat aaaatatgt gaagtata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY