Status
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Gene information
General information
TC012910 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451215.1: 43575..53881
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012910
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06659

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 4

    vitellarium complete not present - Number of animals showing the phenotype: 4

    tropharium shortened - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    6

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    labrum shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012910 RA
      MSSRRWFWP TISGIEAER VLMERGVDC SFLARPSSN 
      NPGSFTLSV KRNGEVTHI KIQNSGDFY DLYGGEKFA 
      TLSELVQYY MENQGQLRE KNGEIIELK YPLSCADPT 
      TERWFHGHL SGKEAEKLI LERGKNGSF LVRESQSKP 
      GDFVLSVRT DDKMTHVMI RYSDNQYDV GGGEKFDSL 
      AELIEYYKK NPMVETSGT VVHLKQPFN ATRISASGI 
      HSRVKQLQN ENGPNGMGK AGFWEEFES LQQQEFKHL 
      YSRKEGVKP ENRNKNRYK NILPFDDTR VKLKDADPN 
      VPGSDYINA NYIKWKADY CGGNELGSD PSGKVYIAT 
      QGCLPSTIA DFWQMVWQE NCRVIVMTT KETERGKTK 
      CARYWPDQG TKEYRKIKV KILMESSTP HYTLREFLV 
      SMDGTNCER KVYQYHFQA WPDHGVPSD PGCVLNFLH 
      EVNKRQESL QQELPDNPP GAILVHCSA GIGRTGTFI 
      VIDMILDQL KKYGLDCEI DIQRTIQMV RSQRSGMVQ 
      TEAQYKFVY LAVQHHIET TTERMKAEQ KSMQIGREY 
      TNIRYSSDI GSTLSSSAT SGLGSTTSL SSRSSTLAH 
      FPTPSPNSV ILRGETKKP PRAVSDIPL PRPPDDLVK 
      PTLYENIPV SERKHSGTS SSLNCPVPP PRKAT
    CDS sequence
    • >TC012910 RA
      atgagttcc cgaagatgg ttttggccc acaatcagt 
      gggatagag gctgaaaga gttttaatg gaaagagga 
      gttgattgc agtttcttg gcaagacct agttcaaat 
      aatcccgga tcgttcacg ttatcagtc aagagaaat 
      ggagaggtt acccacatt aaaatccaa aattctgga 
      gacttttat gacctgtat ggcggcgag aaatttgca 
      actctctca gagttggtg cagtattat atggaaaat 
      caggggcaa cttagggag aaaaatggg gaaattatc 
      gaacttaag tatcccctg agctgtgcc gaccccaca 
      actgagaga tggtttcat gggcatttg tccgggaaa 
      gaagcagaa aaattaatt ctagaacga ggtaaaaat 
      ggtagcttt ttagtgcga gaaagtcaa tcgaagcct 
      ggcgatttt gttctttct gttcgaacc gacgacaaa 
      atgactcac gtcatgatt agatattcc gataatcaa 
      tacgatgtt ggtggcggc gagaaattt gatagtttg 
      gctgaactc atcgagtat tacaaaaag aatccgatg 
      gtcgaaact tcaggaaca gtcgttcac ctgaaacag 
      cctttcaat gcaactaga atcagtgct tcggggatt 
      cactcccga gtcaaacaa ttgcaaaac gagaatggt 
      cccaatggc atgggcaaa gccggcttt tgggaggaa 
      ttcgaatcg ttgcaacag caagaattc aaacatttg 
      tattcacgg aaagagggt gttaaacca gagaataga 
      aacaagaat cggtacaag aatattttg ccttttgac 
      gatacacga gtcaagttg aaagatgcg gatcctaat 
      gttcccgga tcggattac attaacgcg aattacatt 
      aaatggaaa gctgattat tgcggaggg aacgaatta 
      ggaagtgat ccaagcgga aaagtgtac attgcaacc 
      caaggctgt ttaccttcc acgatcgcc gatttttgg 
      caaatggta tggcaggag aattgtaga gttatagta 
      atgacaacc aaagagact gaacgaggc aaaacgaag 
      tgtgccagg tattggccg gatcaggga acaaaagag 
      taccgaaaa attaaagtg aagatattg atggagtcg 
      tccactcca cattacact cttagagag tttctagtt 
      agcatggat ggaacaaat tgtgagaga aaagtttac 
      cagtaccat tttcaggca tggccggat catggcgtg 
      ccttcagac ccaggctgt gttttaaat tttttgcat 
      gaggttaat aagagacag gaaagcctt cagcaagag 
      cttccagat aatccccca ggtgctatt ttagtgcat 
      tgttctgct ggtattgga agaactggc acttttatt 
      gttattgat atgatcttg gaccagctt aagaaatac 
      ggtttagac tgtgagata gatatacaa cgcacaatt 
      cagatggtt aggtcccaa cgttctgga atggttcaa 
      actgaagcc caatataaa tttgtttat ttagcagta 
      caacaccat atagagaca actactgaa agaatgaag 
      gctgaacaa aaatcaatg caaataggc cgggagtac 
      acaaacata agatatagc agcgatata ggcagcacg 
      ttatcgtca tcggcgacg agtggttta ggaagtaca 
      acttcgctg tctagtcgg tcttccaca ttggcccac 
      tttccgacc ccatctccc aattctgtg attttaaga 
      ggcgaaaca aagaagcca cctcgagct gtgtcagat 
      attccattg cccaggccg cctgatgat ttggtgaag 
      cctactctg tatgagaac attcctgtc agcgaacgg 
      aaacattca gggacttcg agctctttg aattgtcct 
      gtgccgcct ccgcggaag gcgacgtga
    mRNA sequence
    • >TC012910 RA
      tagataatg ataaagtgt tgtaatagt gcatttcct 
      ggtttacaa aatcatcgt agatagtaa atgcacaag 
      attgttact taattcaca taagaagaa aaaccgtaa 
      attaataaa ataattact gtttctttg agttcagtt 
      cgagttttg ggtcacctg gtatataga aattggtta 
      gagtttttg accgtccct tgtagattc aagatggtg 
      gctcacgta ggagatagt ttttgcaag aggtttatt 
      ttatattca tagattttg taacaaaag tccaaccaa 
      aagtatgag ttcccgaag atggttttg gcccacaat 
      cagtgggat agaggctga aagagtttt aatggaaag 
      aggagttga ttgcagttt cttggcaag acctagttc 
      aaataatcc cggatcgtt cacgttatc agtcaagag 
      aaatggaga ggttaccca cattaaaat ccaaaattc 
      tggagactt ttatgacct gtatggcgg cgagaaatt 
      tgcaactct ctcagagtt ggtgcagta ttatatgga 
      aaatcaggg gcaacttag ggagaaaaa tggggaaat 
      tatcgaact taagtatcc cctgagctg tgccgaccc 
      cacaactga gagatggtt tcatgggca tttgtccgg 
      gaaagaagc agaaaaatt aattctaga acgaggtaa 
      aaatggtag ctttttagt gcgagaaag tcaatcgaa 
      gcctggcga ttttgttct ttctgttcg aaccgacga 
      caaaatgac tcacgtcat gattagata ttccgataa 
      tcaatacga tgttggtgg cggcgagaa atttgatag 
      tttggctga actcatcga gtattacaa aaagaatcc 
      gatggtcga aacttcagg aacagtcgt tcacctgaa 
      acagccttt caatgcaac tagaatcag tgcttcggg 
      gattcactc ccgagtcaa acaattgca aaacgagaa 
      tggtcccaa tggcatggg caaagccgg cttttggga 
      ggaattcga atcgttgca acagcaaga attcaaaca 
      tttgtattc acggaaaga gggtgttaa accagagaa 
      tagaaacaa gaatcggta caagaatat tttgccttt 
      tgacgatac acgagtcaa gttgaaaga tgcggatcc 
      taatgttcc cggatcgga ttacattaa cgcgaatta 
      cattaaatg gaaagctga ttattgcgg agggaacga 
      attaggaag tgatccaag cggaaaagt gtacattgc 
      aacccaagg ctgtttacc ttccacgat cgccgattt 
      ttggcaaat ggtatggca ggagaattg tagagttat 
      agtaatgac aaccaaaga gactgaacg aggcaaaac 
      gaagtgtgc caggtattg gccggatca gggaacaaa 
      agagtaccg aaaaattaa agtgaagat attgatgga 
      gtcgtccac tccacatta cactcttag agagtttct 
      agttagcat ggatggaac aaattgtga gagaaaagt 
      ttaccagta ccattttca ggcatggcc ggatcatgg 
      cgtgccttc agacccagg ctgtgtttt aaatttttt 
      gcatgaggt taataagag acaggaaag ccttcagca 
      agagcttcc agataatcc cccaggtgc tattttagt 
      gcattgttc tgctggtat tggaagaac tggcacttt 
      tattgttat tgatatgat cttggacca gcttaagaa 
      atacggttt agactgtga gatagatat acaacgcac 
      aattcagat ggttaggtc ccaacgttc tggaatggt 
      tcaaactga agcccaata taaatttgt ttatttagc 
      agtacaaca ccatataga gacaactac tgaaagaat 
      gaaggctga acaaaaatc aatgcaaat aggccggga 
      gtacacaaa cataagata tagcagcga tataggcag 
      cacgttatc gtcatcggc gacgagtgg tttaggaag 
      tacaacttc gctgtctag tcggtcttc cacattggc 
      ccactttcc gaccccatc tcccaattc tgtgatttt 
      aagaggcga aacaaagaa gccacctcg agctgtgtc 
      agatattcc attgcccag gccgcctga tgatttggt 
      gaagcctac tctgtatga gaacattcc tgtcagcga 
      acggaaaca ttcagggac ttcgagctc tttgaattg 
      tcctgtgcc gcctccgcg gaaggcgac gtgacatag 
      acgaacttt taattccaa atttacttc atgtacctt 
      ctcagtgtg ctaggaaaa attatcaag tcaaattaa 
      gatgcattg ttcaagagc ctagttgat gtatcattg 
      ttaaaagaa tgccagtat ttacaacct tgaagagat 
      tgtgagtgt tactaaaag tacctaccc tttaagtaa 
      cacaatctg acgaaatta tttttagag cacagttgt 
      atatattca atataatta taccaccat acgtatttt 
      tatcttgaa tgtctcgat taacatgtg tattttatt 
      tttatttct tagttttgc catttcgct tttgatgcc 
      taaagcaac ggtaagtat tttaggcat acgcttttt 
      gtttgattc atttccaaa gatctgaag agagtttaa 
      gcttagttg gcaacaagt cacttggtg atttttcat 
      gattatttt taagtttaa gtaaattgt gtattttta 
      tatttttgc taaattatt tatatattc aaagcacac 
      ttagtgata caccttctt aatacaggt ggggtgcta 
      tttatgttc tttttatta ccacctgta tcaaataca 
      gatttgaca atattatat cttatcttt aaatagatg 
      tttgcataa aacagtttt attttgatc gttttacaa 
      caaactggt gctaagttt tgcttcttc tgttatcat 
      aagaattta gtcagatca aaggttttg tcaactttc 
      tgtcgtcta caatatatg tttagaaat ttttactct 
      cttacttat aaaacagga cccaaacca ctttcccgc 
      aacaagaag aataaaatg tttttttta at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY