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Gene information
General information
TC012910 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451215.1: 43575..53881
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012910
    iBeetle screen iB_06659

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 4

    vitellarium complete not present - Number of animals showing the phenotype: 4

    tropharium shortened - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    6
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    labrum shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012910 RA
      MSSRRWFWP TISGIEAER VLMERGVDC SFLARPSSN 
NPGSFTLSV KRNGEVTHI KIQNSGDFY DLYGGEKFA 
TLSELVQYY MENQGQLRE KNGEIIELK YPLSCADPT 
TERWFHGHL SGKEAEKLI LERGKNGSF LVRESQSKP 
GDFVLSVRT DDKMTHVMI RYSDNQYDV GGGEKFDSL 
AELIEYYKK NPMVETSGT VVHLKQPFN ATRISASGI 
HSRVKQLQN ENGPNGMGK AGFWEEFES LQQQEFKHL 
YSRKEGVKP ENRNKNRYK NILPFDDTR VKLKDADPN 
VPGSDYINA NYIKWKADY CGGNELGSD PSGKVYIAT 
QGCLPSTIA DFWQMVWQE NCRVIVMTT KETERGKTK 
CARYWPDQG TKEYRKIKV KILMESSTP HYTLREFLV 
SMDGTNCER KVYQYHFQA WPDHGVPSD PGCVLNFLH 
EVNKRQESL QQELPDNPP GAILVHCSA GIGRTGTFI 
VIDMILDQL KKYGLDCEI DIQRTIQMV RSQRSGMVQ 
TEAQYKFVY LAVQHHIET TTERMKAEQ KSMQIGREY 
TNIRYSSDI GSTLSSSAT SGLGSTTSL SSRSSTLAH 
FPTPSPNSV ILRGETKKP PRAVSDIPL PRPPDDLVK 
PTLYENIPV SERKHSGTS SSLNCPVPP PRKAT
    CDS sequence
    • >TC012910 RA
      atgagttcc cgaagatgg ttttggccc acaatcagt 
gggatagag gctgaaaga gttttaatg gaaagagga 
gttgattgc agtttcttg gcaagacct agttcaaat 
aatcccgga tcgttcacg ttatcagtc aagagaaat 
ggagaggtt acccacatt aaaatccaa aattctgga 
gacttttat gacctgtat ggcggcgag aaatttgca 
actctctca gagttggtg cagtattat atggaaaat 
caggggcaa cttagggag aaaaatggg gaaattatc 
gaacttaag tatcccctg agctgtgcc gaccccaca 
actgagaga tggtttcat gggcatttg tccgggaaa 
gaagcagaa aaattaatt ctagaacga ggtaaaaat 
ggtagcttt ttagtgcga gaaagtcaa tcgaagcct 
ggcgatttt gttctttct gttcgaacc gacgacaaa 
atgactcac gtcatgatt agatattcc gataatcaa 
tacgatgtt ggtggcggc gagaaattt gatagtttg 
gctgaactc atcgagtat tacaaaaag aatccgatg 
gtcgaaact tcaggaaca gtcgttcac ctgaaacag 
cctttcaat gcaactaga atcagtgct tcggggatt 
cactcccga gtcaaacaa ttgcaaaac gagaatggt 
cccaatggc atgggcaaa gccggcttt tgggaggaa 
ttcgaatcg ttgcaacag caagaattc aaacatttg 
tattcacgg aaagagggt gttaaacca gagaataga 
aacaagaat cggtacaag aatattttg ccttttgac 
gatacacga gtcaagttg aaagatgcg gatcctaat 
gttcccgga tcggattac attaacgcg aattacatt 
aaatggaaa gctgattat tgcggaggg aacgaatta 
ggaagtgat ccaagcgga aaagtgtac attgcaacc 
caaggctgt ttaccttcc acgatcgcc gatttttgg 
caaatggta tggcaggag aattgtaga gttatagta 
atgacaacc aaagagact gaacgaggc aaaacgaag 
tgtgccagg tattggccg gatcaggga acaaaagag 
taccgaaaa attaaagtg aagatattg atggagtcg 
tccactcca cattacact cttagagag tttctagtt 
agcatggat ggaacaaat tgtgagaga aaagtttac 
cagtaccat tttcaggca tggccggat catggcgtg 
ccttcagac ccaggctgt gttttaaat tttttgcat 
gaggttaat aagagacag gaaagcctt cagcaagag 
cttccagat aatccccca ggtgctatt ttagtgcat 
tgttctgct ggtattgga agaactggc acttttatt 
gttattgat atgatcttg gaccagctt aagaaatac 
ggtttagac tgtgagata gatatacaa cgcacaatt 
cagatggtt aggtcccaa cgttctgga atggttcaa 
actgaagcc caatataaa tttgtttat ttagcagta 
caacaccat atagagaca actactgaa agaatgaag 
gctgaacaa aaatcaatg caaataggc cgggagtac 
acaaacata agatatagc agcgatata ggcagcacg 
ttatcgtca tcggcgacg agtggttta ggaagtaca 
acttcgctg tctagtcgg tcttccaca ttggcccac 
tttccgacc ccatctccc aattctgtg attttaaga 
ggcgaaaca aagaagcca cctcgagct gtgtcagat 
attccattg cccaggccg cctgatgat ttggtgaag 
cctactctg tatgagaac attcctgtc agcgaacgg 
aaacattca gggacttcg agctctttg aattgtcct 
gtgccgcct ccgcggaag gcgacgtga
    mRNA sequence
    • >TC012910 RA
      tagataatg ataaagtgt tgtaatagt gcatttcct 
ggtttacaa aatcatcgt agatagtaa atgcacaag 
attgttact taattcaca taagaagaa aaaccgtaa 
attaataaa ataattact gtttctttg agttcagtt 
cgagttttg ggtcacctg gtatataga aattggtta 
gagtttttg accgtccct tgtagattc aagatggtg 
gctcacgta ggagatagt ttttgcaag aggtttatt 
ttatattca tagattttg taacaaaag tccaaccaa 
aagtatgag ttcccgaag atggttttg gcccacaat 
cagtgggat agaggctga aagagtttt aatggaaag 
aggagttga ttgcagttt cttggcaag acctagttc 
aaataatcc cggatcgtt cacgttatc agtcaagag 
aaatggaga ggttaccca cattaaaat ccaaaattc 
tggagactt ttatgacct gtatggcgg cgagaaatt 
tgcaactct ctcagagtt ggtgcagta ttatatgga 
aaatcaggg gcaacttag ggagaaaaa tggggaaat 
tatcgaact taagtatcc cctgagctg tgccgaccc 
cacaactga gagatggtt tcatgggca tttgtccgg 
gaaagaagc agaaaaatt aattctaga acgaggtaa 
aaatggtag ctttttagt gcgagaaag tcaatcgaa 
gcctggcga ttttgttct ttctgttcg aaccgacga 
caaaatgac tcacgtcat gattagata ttccgataa 
tcaatacga tgttggtgg cggcgagaa atttgatag 
tttggctga actcatcga gtattacaa aaagaatcc 
gatggtcga aacttcagg aacagtcgt tcacctgaa 
acagccttt caatgcaac tagaatcag tgcttcggg 
gattcactc ccgagtcaa acaattgca aaacgagaa 
tggtcccaa tggcatggg caaagccgg cttttggga 
ggaattcga atcgttgca acagcaaga attcaaaca 
tttgtattc acggaaaga gggtgttaa accagagaa 
tagaaacaa gaatcggta caagaatat tttgccttt 
tgacgatac acgagtcaa gttgaaaga tgcggatcc 
taatgttcc cggatcgga ttacattaa cgcgaatta 
cattaaatg gaaagctga ttattgcgg agggaacga 
attaggaag tgatccaag cggaaaagt gtacattgc 
aacccaagg ctgtttacc ttccacgat cgccgattt 
ttggcaaat ggtatggca ggagaattg tagagttat 
agtaatgac aaccaaaga gactgaacg aggcaaaac 
gaagtgtgc caggtattg gccggatca gggaacaaa 
agagtaccg aaaaattaa agtgaagat attgatgga 
gtcgtccac tccacatta cactcttag agagtttct 
agttagcat ggatggaac aaattgtga gagaaaagt 
ttaccagta ccattttca ggcatggcc ggatcatgg 
cgtgccttc agacccagg ctgtgtttt aaatttttt 
gcatgaggt taataagag acaggaaag ccttcagca 
agagcttcc agataatcc cccaggtgc tattttagt 
gcattgttc tgctggtat tggaagaac tggcacttt 
tattgttat tgatatgat cttggacca gcttaagaa 
atacggttt agactgtga gatagatat acaacgcac 
aattcagat ggttaggtc ccaacgttc tggaatggt 
tcaaactga agcccaata taaatttgt ttatttagc 
agtacaaca ccatataga gacaactac tgaaagaat 
gaaggctga acaaaaatc aatgcaaat aggccggga 
gtacacaaa cataagata tagcagcga tataggcag 
cacgttatc gtcatcggc gacgagtgg tttaggaag 
tacaacttc gctgtctag tcggtcttc cacattggc 
ccactttcc gaccccatc tcccaattc tgtgatttt 
aagaggcga aacaaagaa gccacctcg agctgtgtc 
agatattcc attgcccag gccgcctga tgatttggt 
gaagcctac tctgtatga gaacattcc tgtcagcga 
acggaaaca ttcagggac ttcgagctc tttgaattg 
tcctgtgcc gcctccgcg gaaggcgac gtgacatag 
acgaacttt taattccaa atttacttc atgtacctt 
ctcagtgtg ctaggaaaa attatcaag tcaaattaa 
gatgcattg ttcaagagc ctagttgat gtatcattg 
ttaaaagaa tgccagtat ttacaacct tgaagagat 
tgtgagtgt tactaaaag tacctaccc tttaagtaa 
cacaatctg acgaaatta tttttagag cacagttgt 
atatattca atataatta taccaccat acgtatttt 
tatcttgaa tgtctcgat taacatgtg tattttatt 
tttatttct tagttttgc catttcgct tttgatgcc 
taaagcaac ggtaagtat tttaggcat acgcttttt 
gtttgattc atttccaaa gatctgaag agagtttaa 
gcttagttg gcaacaagt cacttggtg atttttcat 
gattatttt taagtttaa gtaaattgt gtattttta 
tatttttgc taaattatt tatatattc aaagcacac 
ttagtgata caccttctt aatacaggt ggggtgcta 
tttatgttc tttttatta ccacctgta tcaaataca 
gatttgaca atattatat cttatcttt aaatagatg 
tttgcataa aacagtttt attttgatc gttttacaa 
caaactggt gctaagttt tgcttcttc tgttatcat 
aagaattta gtcagatca aaggttttg tcaactttc 
tgtcgtcta caatatatg tttagaaat ttttactct 
cttacttat aaaacagga cccaaacca ctttcccgc 
aacaagaag aataaaatg tttttttta at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY