General information | |||
TC012910 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451215.1: 43575..53881 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC012910 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06659 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
vitellarium complete not present - Number of animals showing the phenotype: 4
tropharium shortened - Number of animals showing the phenotype: 4
6 |
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%
labrum shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
legs potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
legs everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012910 RA
MSSRRWFWP TISGIEAER VLMERGVDC SFLARPSSN NPGSFTLSV KRNGEVTHI KIQNSGDFY DLYGGEKFA TLSELVQYY MENQGQLRE KNGEIIELK YPLSCADPT TERWFHGHL SGKEAEKLI LERGKNGSF LVRESQSKP GDFVLSVRT DDKMTHVMI RYSDNQYDV GGGEKFDSL AELIEYYKK NPMVETSGT VVHLKQPFN ATRISASGI HSRVKQLQN ENGPNGMGK AGFWEEFES LQQQEFKHL YSRKEGVKP ENRNKNRYK NILPFDDTR VKLKDADPN VPGSDYINA NYIKWKADY CGGNELGSD PSGKVYIAT QGCLPSTIA DFWQMVWQE NCRVIVMTT KETERGKTK CARYWPDQG TKEYRKIKV KILMESSTP HYTLREFLV SMDGTNCER KVYQYHFQA WPDHGVPSD PGCVLNFLH EVNKRQESL QQELPDNPP GAILVHCSA GIGRTGTFI VIDMILDQL KKYGLDCEI DIQRTIQMV RSQRSGMVQ TEAQYKFVY LAVQHHIET TTERMKAEQ KSMQIGREY TNIRYSSDI GSTLSSSAT SGLGSTTSL SSRSSTLAH FPTPSPNSV ILRGETKKP PRAVSDIPL PRPPDDLVK PTLYENIPV SERKHSGTS SSLNCPVPP PRKAT
- >TC012910 RA
atgagttcc cgaagatgg ttttggccc acaatcagt gggatagag gctgaaaga gttttaatg gaaagagga gttgattgc agtttcttg gcaagacct agttcaaat aatcccgga tcgttcacg ttatcagtc aagagaaat ggagaggtt acccacatt aaaatccaa aattctgga gacttttat gacctgtat ggcggcgag aaatttgca actctctca gagttggtg cagtattat atggaaaat caggggcaa cttagggag aaaaatggg gaaattatc gaacttaag tatcccctg agctgtgcc gaccccaca actgagaga tggtttcat gggcatttg tccgggaaa gaagcagaa aaattaatt ctagaacga ggtaaaaat ggtagcttt ttagtgcga gaaagtcaa tcgaagcct ggcgatttt gttctttct gttcgaacc gacgacaaa atgactcac gtcatgatt agatattcc gataatcaa tacgatgtt ggtggcggc gagaaattt gatagtttg gctgaactc atcgagtat tacaaaaag aatccgatg gtcgaaact tcaggaaca gtcgttcac ctgaaacag cctttcaat gcaactaga atcagtgct tcggggatt cactcccga gtcaaacaa ttgcaaaac gagaatggt cccaatggc atgggcaaa gccggcttt tgggaggaa ttcgaatcg ttgcaacag caagaattc aaacatttg tattcacgg aaagagggt gttaaacca gagaataga aacaagaat cggtacaag aatattttg ccttttgac gatacacga gtcaagttg aaagatgcg gatcctaat gttcccgga tcggattac attaacgcg aattacatt aaatggaaa gctgattat tgcggaggg aacgaatta ggaagtgat ccaagcgga aaagtgtac attgcaacc caaggctgt ttaccttcc acgatcgcc gatttttgg caaatggta tggcaggag aattgtaga gttatagta atgacaacc aaagagact gaacgaggc aaaacgaag tgtgccagg tattggccg gatcaggga acaaaagag taccgaaaa attaaagtg aagatattg atggagtcg tccactcca cattacact cttagagag tttctagtt agcatggat ggaacaaat tgtgagaga aaagtttac cagtaccat tttcaggca tggccggat catggcgtg ccttcagac ccaggctgt gttttaaat tttttgcat gaggttaat aagagacag gaaagcctt cagcaagag cttccagat aatccccca ggtgctatt ttagtgcat tgttctgct ggtattgga agaactggc acttttatt gttattgat atgatcttg gaccagctt aagaaatac ggtttagac tgtgagata gatatacaa cgcacaatt cagatggtt aggtcccaa cgttctgga atggttcaa actgaagcc caatataaa tttgtttat ttagcagta caacaccat atagagaca actactgaa agaatgaag gctgaacaa aaatcaatg caaataggc cgggagtac acaaacata agatatagc agcgatata ggcagcacg ttatcgtca tcggcgacg agtggttta ggaagtaca acttcgctg tctagtcgg tcttccaca ttggcccac tttccgacc ccatctccc aattctgtg attttaaga ggcgaaaca aagaagcca cctcgagct gtgtcagat attccattg cccaggccg cctgatgat ttggtgaag cctactctg tatgagaac attcctgtc agcgaacgg aaacattca gggacttcg agctctttg aattgtcct gtgccgcct ccgcggaag gcgacgtga
- >TC012910 RA
tagataatg ataaagtgt tgtaatagt gcatttcct ggtttacaa aatcatcgt agatagtaa atgcacaag attgttact taattcaca taagaagaa aaaccgtaa attaataaa ataattact gtttctttg agttcagtt cgagttttg ggtcacctg gtatataga aattggtta gagtttttg accgtccct tgtagattc aagatggtg gctcacgta ggagatagt ttttgcaag aggtttatt ttatattca tagattttg taacaaaag tccaaccaa aagtatgag ttcccgaag atggttttg gcccacaat cagtgggat agaggctga aagagtttt aatggaaag aggagttga ttgcagttt cttggcaag acctagttc aaataatcc cggatcgtt cacgttatc agtcaagag aaatggaga ggttaccca cattaaaat ccaaaattc tggagactt ttatgacct gtatggcgg cgagaaatt tgcaactct ctcagagtt ggtgcagta ttatatgga aaatcaggg gcaacttag ggagaaaaa tggggaaat tatcgaact taagtatcc cctgagctg tgccgaccc cacaactga gagatggtt tcatgggca tttgtccgg gaaagaagc agaaaaatt aattctaga acgaggtaa aaatggtag ctttttagt gcgagaaag tcaatcgaa gcctggcga ttttgttct ttctgttcg aaccgacga caaaatgac tcacgtcat gattagata ttccgataa tcaatacga tgttggtgg cggcgagaa atttgatag tttggctga actcatcga gtattacaa aaagaatcc gatggtcga aacttcagg aacagtcgt tcacctgaa acagccttt caatgcaac tagaatcag tgcttcggg gattcactc ccgagtcaa acaattgca aaacgagaa tggtcccaa tggcatggg caaagccgg cttttggga ggaattcga atcgttgca acagcaaga attcaaaca tttgtattc acggaaaga gggtgttaa accagagaa tagaaacaa gaatcggta caagaatat tttgccttt tgacgatac acgagtcaa gttgaaaga tgcggatcc taatgttcc cggatcgga ttacattaa cgcgaatta cattaaatg gaaagctga ttattgcgg agggaacga attaggaag tgatccaag cggaaaagt gtacattgc aacccaagg ctgtttacc ttccacgat cgccgattt ttggcaaat ggtatggca ggagaattg tagagttat agtaatgac aaccaaaga gactgaacg aggcaaaac gaagtgtgc caggtattg gccggatca gggaacaaa agagtaccg aaaaattaa agtgaagat attgatgga gtcgtccac tccacatta cactcttag agagtttct agttagcat ggatggaac aaattgtga gagaaaagt ttaccagta ccattttca ggcatggcc ggatcatgg cgtgccttc agacccagg ctgtgtttt aaatttttt gcatgaggt taataagag acaggaaag ccttcagca agagcttcc agataatcc cccaggtgc tattttagt gcattgttc tgctggtat tggaagaac tggcacttt tattgttat tgatatgat cttggacca gcttaagaa atacggttt agactgtga gatagatat acaacgcac aattcagat ggttaggtc ccaacgttc tggaatggt tcaaactga agcccaata taaatttgt ttatttagc agtacaaca ccatataga gacaactac tgaaagaat gaaggctga acaaaaatc aatgcaaat aggccggga gtacacaaa cataagata tagcagcga tataggcag cacgttatc gtcatcggc gacgagtgg tttaggaag tacaacttc gctgtctag tcggtcttc cacattggc ccactttcc gaccccatc tcccaattc tgtgatttt aagaggcga aacaaagaa gccacctcg agctgtgtc agatattcc attgcccag gccgcctga tgatttggt gaagcctac tctgtatga gaacattcc tgtcagcga acggaaaca ttcagggac ttcgagctc tttgaattg tcctgtgcc gcctccgcg gaaggcgac gtgacatag acgaacttt taattccaa atttacttc atgtacctt ctcagtgtg ctaggaaaa attatcaag tcaaattaa gatgcattg ttcaagagc ctagttgat gtatcattg ttaaaagaa tgccagtat ttacaacct tgaagagat tgtgagtgt tactaaaag tacctaccc tttaagtaa cacaatctg acgaaatta tttttagag cacagttgt atatattca atataatta taccaccat acgtatttt tatcttgaa tgtctcgat taacatgtg tattttatt tttatttct tagttttgc catttcgct tttgatgcc taaagcaac ggtaagtat tttaggcat acgcttttt gtttgattc atttccaaa gatctgaag agagtttaa gcttagttg gcaacaagt cacttggtg atttttcat gattatttt taagtttaa gtaaattgt gtattttta tatttttgc taaattatt tatatattc aaagcacac ttagtgata caccttctt aatacaggt ggggtgcta tttatgttc tttttatta ccacctgta tcaaataca gatttgaca atattatat cttatcttt aaatagatg tttgcataa aacagtttt attttgatc gttttacaa caaactggt gctaagttt tgcttcttc tgttatcat aagaattta gtcagatca aaggttttg tcaactttc tgtcgtcta caatatatg tttagaaat ttttactct cttacttat aaaacagga cccaaacca ctttcccgc aacaagaag aataaaatg tttttttta at
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
