| General information | |||
| TC004274 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3899446..3909847 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004274 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06652 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC004274 RA
MWSKVILFV FFVNYGNSA NILGLYFHA GKSHHILGE MLLKELARR GHNVTMASP FPLKEPFPN YTDIHLTGI LEDQIARES MFMKMNKGH ALVGVNDVL ELTRAQTEL TLNHTEMAK LLKSGTKFD VIIIDWFMN GAILMYGKL FNAPIIPIA SHGTTHLAN YIVGNPAPP SYIPNAMLP FPPNMTFFQ RMVNGLVTI AYNVVGHTN AKYHQALLE KYFENAPSL DELKDTVAL VLSNGHYSF ESPRPFVPN VIPVGGFHV QKARKLPTD LQKYMDEAK HGVVYFSLG SNMKSVLLP KEKQQQILK AFAKIPHKV LWKWEDDNL ENKPDNVLI RKWFPQNDI LGHPNLKLF ITHGGLLST IEALHHGVP VLGIPIFGD QKANIPNAV SSGYAVQLE LADLDEATL TKALDEILT NPKYQENAK KRSQLLHDQ PMSPMDTAV FWVEHVIRH KGAPHLRNL GSYLPWYQY LMLDVALFV VLFLLVVVG IVGFVIRKI CGCCCQRKS KEKRDALRG AAAAASRRM SGCDPVCTK RQCSSVLNM FTLCMSRGH PPLQNAQQE NNVANKQVK GGYLLRYKR GLFCNSWCE EWFVLYEDS TLAWFKDKG LNRPKGHLR ISECPDLLA VGEWTRQVP KRPRFPRNY HVGQVLAVG TRIPQDVHW LMAQSAAEA NDWMTAISN TLPPPPNLP PAKSKSFTF QKKSNNCYK CNSLKNEAD YTLISGVAI DWGHGWGWG QTAWAAHTA CVSETATAL EAAVNSALH CHALGDYGV GSGMDELDC SAFGDFIF
- >TC004274 RA
atgtggtcc aaagtgatt ttgttcgtg ttttttgtt aattatggc aatagtgcc aatattctg gggttgtat tttcacgcg gggaaaagt caccacatt cttggcgaa atgctcctg aaagaattg gccaggagg gggcataat gtaacgatg gctagtccg tttccactc aaggaaccg ttcccgaat tataccgat attcacttg accggtatt ttggaagat caaatagca agagagagt atgtttatg aaaatgaac aaaggccat gccctggtt ggcgtcaac gacgtgctt gagttaaca agagcccaa accgagctc accctcaac cacaccgaa atggccaag ttgctaaaa tccggaaca aaattcgac gttatcata attgactgg ttcatgaac ggtgcaatt ttaatgtat gggaagctc ttcaacgca cccatcatc cccatagcc tcccatgga accacccat ttggccaac tacatcgtg ggaaaccca gccccgcca tcttacatc ccgaacgcc atgttgccc ttcccgccc aacatgacg ttcttccaa cggatggtc aatgggctc gttaccatc gcctacaac gtggtgggt cacaccaac gccaagtac caccaagct ctcttggaa aaatatttc gaaaatgcg ccaagtctc gacgaattg aaagacact gtggctttg gttttgtcc aacggacat tacagtttt gaatctccg agacctttc gtccctaat gtgattcca gtcggtggg tttcacgtc caaaaagca cgaaaacta ccaactgat ctccagaaa tatatggat gaggccaaa catggagtg gtttatttc agtcttggg tctaacatg aaaagtgtc ttacttccg aaagagaaa cagcaacaa attcttaaa gcgtttgcc aaaattccg cacaaagtt ttgtggaaa tgggaggat gataatttg gaaaacaaa ccagacaat gtcttgatc cgcaagtgg tttccacaa aatgacatt ctaggccac ccaaatttg aaattgttc ataacccat ggtggcctc ttgagcacc attgaagcg ctacaccat ggggtccca gtcctggga atcccaatt ttcggtgac cagaaagca aacatcccc aacgccgtc tcctccgga tacgccgtc cagttagag ttggccgat ttggacgaa gccacctta accaaagcc ttggacgag atcttaacc aaccccaaa taccaggaa aacgcgaaa aagcgctcc caactcctg cacgaccag cccatgtcg cccatggac acggccgtt ttctgggtg gagcacgtg atccggcac aaaggcgcc ccgcatttg cgaaatctg ggcagttac ctgccctgg taccagtac ctgatgctg gacgtggcg ctgtttgtg gttttattt ttgctagtg gttgttggt attgtcggg tttgttatt aggaaaatt tgcggttgt tgttgtcag aggaaaagt aaggaaaaa agggatgca ttgagaggg gcggcagcg gcggcgtcg cgacgcatg tctgggtgt gatcctgtc tgtaccaaa cgacagtgt tcatcagtg ctcaatatg tttacgttg tgtatgtcc cgagggcat cctccctta caaaacgca caacaggaa aacaacgtt gccaacaag caagtcaaa ggcggatac ctcctccga tataaacga gggttattt tgtaacagc tggtgtgaa gagtggttc gtgttgtac gaagactca actttggct tggtttaaa gacaagggc ttgaaccgc ccaaagggt cacctacgt attagcgaa tgcccggac ttactagcc gtgggcgaa tggacacgt caagtgccc aaaagaccc cgattcccg cgaaactac cacgtagga caagtccta gccgttggc acaaggata ccccaggac gtgcactgg cttatggca cagtcggca gccgaagcc aacgactgg atgaccgca attagcaat actttgccg cctcctcca aacttgccc ccagccaag agcaagagt ttcacgttc cagaagaaa agcaacaat tgttacaaa tgcaattct ctgaagaac gaggctgat tatacgctg atttcggga gtggccatc gactggggc cacggctgg ggctggggg cagacggcc tgggctgcc cacacggcc tgtgtctct gagacggcg actgcactg gaggctgcc gtgaattcg gcgctccat tgccatgcc ttgggggat tatggcgtt ggaagtgga atggacgag ctcgactgt agtgcattt ggggacttt attttctaa
- >TC004274 RA
tgaatcaaa attcaatca ctgtaagtg attccagtc caagggaaa atgtggtcc aaagtgatt ttgttcgtg ttttttgtt aattatggc aatagtgcc aatattctg gggttgtat tttcacgcg gggaaaagt caccacatt cttggcgaa atgctcctg aaagaattg gccaggagg gggcataat gtaacgatg gctagtccg tttccactc aaggaaccg ttcccgaat tataccgat attcacttg accggtatt ttggaagat caaatagca agagagagt atgtttatg aaaatgaac aaaggccat gccctggtt ggcgtcaac gacgtgctt gagttaaca agagcccaa accgagctc accctcaac cacaccgaa atggccaag ttgctaaaa tccggaaca aaattcgac gttatcata attgactgg ttcatgaac ggtgcaatt ttaatgtat gggaagctc ttcaacgca cccatcatc cccatagcc tcccatgga accacccat ttggccaac tacatcgtg ggaaaccca gccccgcca tcttacatc ccgaacgcc atgttgccc ttcccgccc aacatgacg ttcttccaa cggatggtc aatgggctc gttaccatc gcctacaac gtggtgggt cacaccaac gccaagtac caccaagct ctcttggaa aaatatttc gaaaatgcg ccaagtctc gacgaattg aaagacact gtggctttg gttttgtcc aacggacat tacagtttt gaatctccg agacctttc gtccctaat gtgattcca gtcggtggg tttcacgtc caaaaagca cgaaaacta ccaactgat ctccagaaa tatatggat gaggccaaa catggagtg gtttatttc agtcttggg tctaacatg aaaagtgtc ttacttccg aaagagaaa cagcaacaa attcttaaa gcgtttgcc aaaattccg cacaaagtt ttgtggaaa tgggaggat gataatttg gaaaacaaa ccagacaat gtcttgatc cgcaagtgg tttccacaa aatgacatt ctaggccac ccaaatttg aaattgttc ataacccat ggtggcctc ttgagcacc attgaagcg ctacaccat ggggtccca gtcctggga atcccaatt ttcggtgac cagaaagca aacatcccc aacgccgtc tcctccgga tacgccgtc cagttagag ttggccgat ttggacgaa gccacctta accaaagcc ttggacgag atcttaacc aaccccaaa taccaggaa aacgcgaaa aagcgctcc caactcctg cacgaccag cccatgtcg cccatggac acggccgtt ttctgggtg gagcacgtg atccggcac aaaggcgcc ccgcatttg cgaaatctg ggcagttac ctgccctgg taccagtac ctgatgctg gacgtggcg ctgtttgtg gttttattt ttgctagtg gttgttggt attgtcggg tttgttatt aggaaaatt tgcggttgt tgttgtcag aggaaaagt aaggaaaaa agggatgca ttgagaggg gcggcagcg gcggcgtcg cgacgcatg tctgggtgt gatcctgtc tgtaccaaa cgacagtgt tcatcagtg ctcaatatg tttacgttg tgtatgtcc cgagggcat cctccctta caaaacgca caacaggaa aacaacgtt gccaacaag caagtcaaa ggcggatac ctcctccga tataaacga gggttattt tgtaacagc tggtgtgaa gagtggttc gtgttgtac gaagactca actttggct tggtttaaa gacaagggc ttgaaccgc ccaaagggt cacctacgt attagcgaa tgcccggac ttactagcc gtgggcgaa tggacacgt caagtgccc aaaagaccc cgattcccg cgaaactac cacgtagga caagtccta gccgttggc acaaggata ccccaggac gtgcactgg cttatggca cagtcggca gccgaagcc aacgactgg atgaccgca attagcaat actttgccg cctcctcca aacttgccc ccagccaag agcaagagt ttcacgttc cagaagaaa agcaacaat tgttacaaa tgcaattct ctgaagaac gaggctgat tatacgctg atttcggga gtggccatc gactggggc cacggctgg ggctggggg cagacggcc tgggctgcc cacacggcc tgtgtctct gagacggcg actgcactg gaggctgcc gtgaattcg gcgctccat tgccatgcc ttgggggat tatggcgtt ggaagtgga atggacgag ctcgactgt agtgcattt ggggacttt attttctaa gcaggaatc aaaaatttg tacctactt tgcagtcaa aacgtaaca attctaaac tagggtact tatttcgcg gcgaaacgc tcaatattt catagacaa ctgacctga attactcat ttcatgatt tacttccgt ccagacaaa attaatttg cgtcgtaat tatgaattc gtactccga ctatttcta aacatgtaa ctgtcattc cttcacacg agcaataaa ctctcgcta ggacattct aaaatgaac cgaacgttt ccattattc atgaaataa gtgtaatgg tgaccgtca tatccataa ctctctgaa tgtacgtta gattacaca aaaactcca tgattaaag gtgctctaa ccgtaagat ttgcttata gttgttgtt agaattgta aatgttttg tagatatac atgaatgta taaggatat ttttgttag attagtact ggaaactta gactgatgt aactatacc agaaatatt tatacatgt gttgcgtct gctagggac gttcgtata aatccagac tgccggtct aaaaattaa atgtcttag cattgaaac gcacatagt taaagactg agtgcgaga gaattaaat gttctaata agtatttaa aatgtaagc caaactcca accctgcta gctagaggg agtaaatga gagcataat acattcatc aagtggtca aaattttgt actttagga taattggga ccaaacaac gttgtttat ttcttcact caaatctct ttgtgtttc taaacagtg aattaatta cttcgacaa tccacgtgc attcaaatg ttctcatta tcgatgtca atacatgtg tgtaaaatg caagtgtgt aatcattat tgtgatatc tgtataagt agctattcc taatttaat aaataataa acaggtaa
