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Gene information
General information
TC004274 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3899446..3909847
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004274
    iBeetle screen iB_06652

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004274 RA
      MWSKVILFV FFVNYGNSA NILGLYFHA GKSHHILGE 
MLLKELARR GHNVTMASP FPLKEPFPN YTDIHLTGI 
LEDQIARES MFMKMNKGH ALVGVNDVL ELTRAQTEL 
TLNHTEMAK LLKSGTKFD VIIIDWFMN GAILMYGKL 
FNAPIIPIA SHGTTHLAN YIVGNPAPP SYIPNAMLP 
FPPNMTFFQ RMVNGLVTI AYNVVGHTN AKYHQALLE 
KYFENAPSL DELKDTVAL VLSNGHYSF ESPRPFVPN 
VIPVGGFHV QKARKLPTD LQKYMDEAK HGVVYFSLG 
SNMKSVLLP KEKQQQILK AFAKIPHKV LWKWEDDNL 
ENKPDNVLI RKWFPQNDI LGHPNLKLF ITHGGLLST 
IEALHHGVP VLGIPIFGD QKANIPNAV SSGYAVQLE 
LADLDEATL TKALDEILT NPKYQENAK KRSQLLHDQ 
PMSPMDTAV FWVEHVIRH KGAPHLRNL GSYLPWYQY 
LMLDVALFV VLFLLVVVG IVGFVIRKI CGCCCQRKS 
KEKRDALRG AAAAASRRM SGCDPVCTK RQCSSVLNM 
FTLCMSRGH PPLQNAQQE NNVANKQVK GGYLLRYKR 
GLFCNSWCE EWFVLYEDS TLAWFKDKG LNRPKGHLR 
ISECPDLLA VGEWTRQVP KRPRFPRNY HVGQVLAVG 
TRIPQDVHW LMAQSAAEA NDWMTAISN TLPPPPNLP 
PAKSKSFTF QKKSNNCYK CNSLKNEAD YTLISGVAI 
DWGHGWGWG QTAWAAHTA CVSETATAL EAAVNSALH 
CHALGDYGV GSGMDELDC SAFGDFIF
    CDS sequence
    • >TC004274 RA
      atgtggtcc aaagtgatt ttgttcgtg ttttttgtt 
aattatggc aatagtgcc aatattctg gggttgtat 
tttcacgcg gggaaaagt caccacatt cttggcgaa 
atgctcctg aaagaattg gccaggagg gggcataat 
gtaacgatg gctagtccg tttccactc aaggaaccg 
ttcccgaat tataccgat attcacttg accggtatt 
ttggaagat caaatagca agagagagt atgtttatg 
aaaatgaac aaaggccat gccctggtt ggcgtcaac 
gacgtgctt gagttaaca agagcccaa accgagctc 
accctcaac cacaccgaa atggccaag ttgctaaaa 
tccggaaca aaattcgac gttatcata attgactgg 
ttcatgaac ggtgcaatt ttaatgtat gggaagctc 
ttcaacgca cccatcatc cccatagcc tcccatgga 
accacccat ttggccaac tacatcgtg ggaaaccca 
gccccgcca tcttacatc ccgaacgcc atgttgccc 
ttcccgccc aacatgacg ttcttccaa cggatggtc 
aatgggctc gttaccatc gcctacaac gtggtgggt 
cacaccaac gccaagtac caccaagct ctcttggaa 
aaatatttc gaaaatgcg ccaagtctc gacgaattg 
aaagacact gtggctttg gttttgtcc aacggacat 
tacagtttt gaatctccg agacctttc gtccctaat 
gtgattcca gtcggtggg tttcacgtc caaaaagca 
cgaaaacta ccaactgat ctccagaaa tatatggat 
gaggccaaa catggagtg gtttatttc agtcttggg 
tctaacatg aaaagtgtc ttacttccg aaagagaaa 
cagcaacaa attcttaaa gcgtttgcc aaaattccg 
cacaaagtt ttgtggaaa tgggaggat gataatttg 
gaaaacaaa ccagacaat gtcttgatc cgcaagtgg 
tttccacaa aatgacatt ctaggccac ccaaatttg 
aaattgttc ataacccat ggtggcctc ttgagcacc 
attgaagcg ctacaccat ggggtccca gtcctggga 
atcccaatt ttcggtgac cagaaagca aacatcccc 
aacgccgtc tcctccgga tacgccgtc cagttagag 
ttggccgat ttggacgaa gccacctta accaaagcc 
ttggacgag atcttaacc aaccccaaa taccaggaa 
aacgcgaaa aagcgctcc caactcctg cacgaccag 
cccatgtcg cccatggac acggccgtt ttctgggtg 
gagcacgtg atccggcac aaaggcgcc ccgcatttg 
cgaaatctg ggcagttac ctgccctgg taccagtac 
ctgatgctg gacgtggcg ctgtttgtg gttttattt 
ttgctagtg gttgttggt attgtcggg tttgttatt 
aggaaaatt tgcggttgt tgttgtcag aggaaaagt 
aaggaaaaa agggatgca ttgagaggg gcggcagcg 
gcggcgtcg cgacgcatg tctgggtgt gatcctgtc 
tgtaccaaa cgacagtgt tcatcagtg ctcaatatg 
tttacgttg tgtatgtcc cgagggcat cctccctta 
caaaacgca caacaggaa aacaacgtt gccaacaag 
caagtcaaa ggcggatac ctcctccga tataaacga 
gggttattt tgtaacagc tggtgtgaa gagtggttc 
gtgttgtac gaagactca actttggct tggtttaaa 
gacaagggc ttgaaccgc ccaaagggt cacctacgt 
attagcgaa tgcccggac ttactagcc gtgggcgaa 
tggacacgt caagtgccc aaaagaccc cgattcccg 
cgaaactac cacgtagga caagtccta gccgttggc 
acaaggata ccccaggac gtgcactgg cttatggca 
cagtcggca gccgaagcc aacgactgg atgaccgca 
attagcaat actttgccg cctcctcca aacttgccc 
ccagccaag agcaagagt ttcacgttc cagaagaaa 
agcaacaat tgttacaaa tgcaattct ctgaagaac 
gaggctgat tatacgctg atttcggga gtggccatc 
gactggggc cacggctgg ggctggggg cagacggcc 
tgggctgcc cacacggcc tgtgtctct gagacggcg 
actgcactg gaggctgcc gtgaattcg gcgctccat 
tgccatgcc ttgggggat tatggcgtt ggaagtgga 
atggacgag ctcgactgt agtgcattt ggggacttt 
attttctaa
    mRNA sequence
    • >TC004274 RA
      tgaatcaaa attcaatca ctgtaagtg attccagtc 
caagggaaa atgtggtcc aaagtgatt ttgttcgtg 
ttttttgtt aattatggc aatagtgcc aatattctg 
gggttgtat tttcacgcg gggaaaagt caccacatt 
cttggcgaa atgctcctg aaagaattg gccaggagg 
gggcataat gtaacgatg gctagtccg tttccactc 
aaggaaccg ttcccgaat tataccgat attcacttg 
accggtatt ttggaagat caaatagca agagagagt 
atgtttatg aaaatgaac aaaggccat gccctggtt 
ggcgtcaac gacgtgctt gagttaaca agagcccaa 
accgagctc accctcaac cacaccgaa atggccaag 
ttgctaaaa tccggaaca aaattcgac gttatcata 
attgactgg ttcatgaac ggtgcaatt ttaatgtat 
gggaagctc ttcaacgca cccatcatc cccatagcc 
tcccatgga accacccat ttggccaac tacatcgtg 
ggaaaccca gccccgcca tcttacatc ccgaacgcc 
atgttgccc ttcccgccc aacatgacg ttcttccaa 
cggatggtc aatgggctc gttaccatc gcctacaac 
gtggtgggt cacaccaac gccaagtac caccaagct 
ctcttggaa aaatatttc gaaaatgcg ccaagtctc 
gacgaattg aaagacact gtggctttg gttttgtcc 
aacggacat tacagtttt gaatctccg agacctttc 
gtccctaat gtgattcca gtcggtggg tttcacgtc 
caaaaagca cgaaaacta ccaactgat ctccagaaa 
tatatggat gaggccaaa catggagtg gtttatttc 
agtcttggg tctaacatg aaaagtgtc ttacttccg 
aaagagaaa cagcaacaa attcttaaa gcgtttgcc 
aaaattccg cacaaagtt ttgtggaaa tgggaggat 
gataatttg gaaaacaaa ccagacaat gtcttgatc 
cgcaagtgg tttccacaa aatgacatt ctaggccac 
ccaaatttg aaattgttc ataacccat ggtggcctc 
ttgagcacc attgaagcg ctacaccat ggggtccca 
gtcctggga atcccaatt ttcggtgac cagaaagca 
aacatcccc aacgccgtc tcctccgga tacgccgtc 
cagttagag ttggccgat ttggacgaa gccacctta 
accaaagcc ttggacgag atcttaacc aaccccaaa 
taccaggaa aacgcgaaa aagcgctcc caactcctg 
cacgaccag cccatgtcg cccatggac acggccgtt 
ttctgggtg gagcacgtg atccggcac aaaggcgcc 
ccgcatttg cgaaatctg ggcagttac ctgccctgg 
taccagtac ctgatgctg gacgtggcg ctgtttgtg 
gttttattt ttgctagtg gttgttggt attgtcggg 
tttgttatt aggaaaatt tgcggttgt tgttgtcag 
aggaaaagt aaggaaaaa agggatgca ttgagaggg 
gcggcagcg gcggcgtcg cgacgcatg tctgggtgt 
gatcctgtc tgtaccaaa cgacagtgt tcatcagtg 
ctcaatatg tttacgttg tgtatgtcc cgagggcat 
cctccctta caaaacgca caacaggaa aacaacgtt 
gccaacaag caagtcaaa ggcggatac ctcctccga 
tataaacga gggttattt tgtaacagc tggtgtgaa 
gagtggttc gtgttgtac gaagactca actttggct 
tggtttaaa gacaagggc ttgaaccgc ccaaagggt 
cacctacgt attagcgaa tgcccggac ttactagcc 
gtgggcgaa tggacacgt caagtgccc aaaagaccc 
cgattcccg cgaaactac cacgtagga caagtccta 
gccgttggc acaaggata ccccaggac gtgcactgg 
cttatggca cagtcggca gccgaagcc aacgactgg 
atgaccgca attagcaat actttgccg cctcctcca 
aacttgccc ccagccaag agcaagagt ttcacgttc 
cagaagaaa agcaacaat tgttacaaa tgcaattct 
ctgaagaac gaggctgat tatacgctg atttcggga 
gtggccatc gactggggc cacggctgg ggctggggg 
cagacggcc tgggctgcc cacacggcc tgtgtctct 
gagacggcg actgcactg gaggctgcc gtgaattcg 
gcgctccat tgccatgcc ttgggggat tatggcgtt 
ggaagtgga atggacgag ctcgactgt agtgcattt 
ggggacttt attttctaa gcaggaatc aaaaatttg 
tacctactt tgcagtcaa aacgtaaca attctaaac 
tagggtact tatttcgcg gcgaaacgc tcaatattt 
catagacaa ctgacctga attactcat ttcatgatt 
tacttccgt ccagacaaa attaatttg cgtcgtaat 
tatgaattc gtactccga ctatttcta aacatgtaa 
ctgtcattc cttcacacg agcaataaa ctctcgcta 
ggacattct aaaatgaac cgaacgttt ccattattc 
atgaaataa gtgtaatgg tgaccgtca tatccataa 
ctctctgaa tgtacgtta gattacaca aaaactcca 
tgattaaag gtgctctaa ccgtaagat ttgcttata 
gttgttgtt agaattgta aatgttttg tagatatac 
atgaatgta taaggatat ttttgttag attagtact 
ggaaactta gactgatgt aactatacc agaaatatt 
tatacatgt gttgcgtct gctagggac gttcgtata 
aatccagac tgccggtct aaaaattaa atgtcttag 
cattgaaac gcacatagt taaagactg agtgcgaga 
gaattaaat gttctaata agtatttaa aatgtaagc 
caaactcca accctgcta gctagaggg agtaaatga 
gagcataat acattcatc aagtggtca aaattttgt 
actttagga taattggga ccaaacaac gttgtttat 
ttcttcact caaatctct ttgtgtttc taaacagtg 
aattaatta cttcgacaa tccacgtgc attcaaatg 
ttctcatta tcgatgtca atacatgtg tgtaaaatg 
caagtgtgt aatcattat tgtgatatc tgtataagt 
agctattcc taatttaat aaataataa acaggtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY