Status
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Gene information
General information
TC004274 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3899446..3909847
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004274
    iBeetle screen iB_06652

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004274 RA
      MWSKVILFV FFVNYGNSA NILGLYFHA GKSHHILGE 
      MLLKELARR GHNVTMASP FPLKEPFPN YTDIHLTGI 
      LEDQIARES MFMKMNKGH ALVGVNDVL ELTRAQTEL 
      TLNHTEMAK LLKSGTKFD VIIIDWFMN GAILMYGKL 
      FNAPIIPIA SHGTTHLAN YIVGNPAPP SYIPNAMLP 
      FPPNMTFFQ RMVNGLVTI AYNVVGHTN AKYHQALLE 
      KYFENAPSL DELKDTVAL VLSNGHYSF ESPRPFVPN 
      VIPVGGFHV QKARKLPTD LQKYMDEAK HGVVYFSLG 
      SNMKSVLLP KEKQQQILK AFAKIPHKV LWKWEDDNL 
      ENKPDNVLI RKWFPQNDI LGHPNLKLF ITHGGLLST 
      IEALHHGVP VLGIPIFGD QKANIPNAV SSGYAVQLE 
      LADLDEATL TKALDEILT NPKYQENAK KRSQLLHDQ 
      PMSPMDTAV FWVEHVIRH KGAPHLRNL GSYLPWYQY 
      LMLDVALFV VLFLLVVVG IVGFVIRKI CGCCCQRKS 
      KEKRDALRG AAAAASRRM SGCDPVCTK RQCSSVLNM 
      FTLCMSRGH PPLQNAQQE NNVANKQVK GGYLLRYKR 
      GLFCNSWCE EWFVLYEDS TLAWFKDKG LNRPKGHLR 
      ISECPDLLA VGEWTRQVP KRPRFPRNY HVGQVLAVG 
      TRIPQDVHW LMAQSAAEA NDWMTAISN TLPPPPNLP 
      PAKSKSFTF QKKSNNCYK CNSLKNEAD YTLISGVAI 
      DWGHGWGWG QTAWAAHTA CVSETATAL EAAVNSALH 
      CHALGDYGV GSGMDELDC SAFGDFIF
    CDS sequence
    • >TC004274 RA
      atgtggtcc aaagtgatt ttgttcgtg ttttttgtt 
      aattatggc aatagtgcc aatattctg gggttgtat 
      tttcacgcg gggaaaagt caccacatt cttggcgaa 
      atgctcctg aaagaattg gccaggagg gggcataat 
      gtaacgatg gctagtccg tttccactc aaggaaccg 
      ttcccgaat tataccgat attcacttg accggtatt 
      ttggaagat caaatagca agagagagt atgtttatg 
      aaaatgaac aaaggccat gccctggtt ggcgtcaac 
      gacgtgctt gagttaaca agagcccaa accgagctc 
      accctcaac cacaccgaa atggccaag ttgctaaaa 
      tccggaaca aaattcgac gttatcata attgactgg 
      ttcatgaac ggtgcaatt ttaatgtat gggaagctc 
      ttcaacgca cccatcatc cccatagcc tcccatgga 
      accacccat ttggccaac tacatcgtg ggaaaccca 
      gccccgcca tcttacatc ccgaacgcc atgttgccc 
      ttcccgccc aacatgacg ttcttccaa cggatggtc 
      aatgggctc gttaccatc gcctacaac gtggtgggt 
      cacaccaac gccaagtac caccaagct ctcttggaa 
      aaatatttc gaaaatgcg ccaagtctc gacgaattg 
      aaagacact gtggctttg gttttgtcc aacggacat 
      tacagtttt gaatctccg agacctttc gtccctaat 
      gtgattcca gtcggtggg tttcacgtc caaaaagca 
      cgaaaacta ccaactgat ctccagaaa tatatggat 
      gaggccaaa catggagtg gtttatttc agtcttggg 
      tctaacatg aaaagtgtc ttacttccg aaagagaaa 
      cagcaacaa attcttaaa gcgtttgcc aaaattccg 
      cacaaagtt ttgtggaaa tgggaggat gataatttg 
      gaaaacaaa ccagacaat gtcttgatc cgcaagtgg 
      tttccacaa aatgacatt ctaggccac ccaaatttg 
      aaattgttc ataacccat ggtggcctc ttgagcacc 
      attgaagcg ctacaccat ggggtccca gtcctggga 
      atcccaatt ttcggtgac cagaaagca aacatcccc 
      aacgccgtc tcctccgga tacgccgtc cagttagag 
      ttggccgat ttggacgaa gccacctta accaaagcc 
      ttggacgag atcttaacc aaccccaaa taccaggaa 
      aacgcgaaa aagcgctcc caactcctg cacgaccag 
      cccatgtcg cccatggac acggccgtt ttctgggtg 
      gagcacgtg atccggcac aaaggcgcc ccgcatttg 
      cgaaatctg ggcagttac ctgccctgg taccagtac 
      ctgatgctg gacgtggcg ctgtttgtg gttttattt 
      ttgctagtg gttgttggt attgtcggg tttgttatt 
      aggaaaatt tgcggttgt tgttgtcag aggaaaagt 
      aaggaaaaa agggatgca ttgagaggg gcggcagcg 
      gcggcgtcg cgacgcatg tctgggtgt gatcctgtc 
      tgtaccaaa cgacagtgt tcatcagtg ctcaatatg 
      tttacgttg tgtatgtcc cgagggcat cctccctta 
      caaaacgca caacaggaa aacaacgtt gccaacaag 
      caagtcaaa ggcggatac ctcctccga tataaacga 
      gggttattt tgtaacagc tggtgtgaa gagtggttc 
      gtgttgtac gaagactca actttggct tggtttaaa 
      gacaagggc ttgaaccgc ccaaagggt cacctacgt 
      attagcgaa tgcccggac ttactagcc gtgggcgaa 
      tggacacgt caagtgccc aaaagaccc cgattcccg 
      cgaaactac cacgtagga caagtccta gccgttggc 
      acaaggata ccccaggac gtgcactgg cttatggca 
      cagtcggca gccgaagcc aacgactgg atgaccgca 
      attagcaat actttgccg cctcctcca aacttgccc 
      ccagccaag agcaagagt ttcacgttc cagaagaaa 
      agcaacaat tgttacaaa tgcaattct ctgaagaac 
      gaggctgat tatacgctg atttcggga gtggccatc 
      gactggggc cacggctgg ggctggggg cagacggcc 
      tgggctgcc cacacggcc tgtgtctct gagacggcg 
      actgcactg gaggctgcc gtgaattcg gcgctccat 
      tgccatgcc ttgggggat tatggcgtt ggaagtgga 
      atggacgag ctcgactgt agtgcattt ggggacttt 
      attttctaa
    mRNA sequence
    • >TC004274 RA
      tgaatcaaa attcaatca ctgtaagtg attccagtc 
      caagggaaa atgtggtcc aaagtgatt ttgttcgtg 
      ttttttgtt aattatggc aatagtgcc aatattctg 
      gggttgtat tttcacgcg gggaaaagt caccacatt 
      cttggcgaa atgctcctg aaagaattg gccaggagg 
      gggcataat gtaacgatg gctagtccg tttccactc 
      aaggaaccg ttcccgaat tataccgat attcacttg 
      accggtatt ttggaagat caaatagca agagagagt 
      atgtttatg aaaatgaac aaaggccat gccctggtt 
      ggcgtcaac gacgtgctt gagttaaca agagcccaa 
      accgagctc accctcaac cacaccgaa atggccaag 
      ttgctaaaa tccggaaca aaattcgac gttatcata 
      attgactgg ttcatgaac ggtgcaatt ttaatgtat 
      gggaagctc ttcaacgca cccatcatc cccatagcc 
      tcccatgga accacccat ttggccaac tacatcgtg 
      ggaaaccca gccccgcca tcttacatc ccgaacgcc 
      atgttgccc ttcccgccc aacatgacg ttcttccaa 
      cggatggtc aatgggctc gttaccatc gcctacaac 
      gtggtgggt cacaccaac gccaagtac caccaagct 
      ctcttggaa aaatatttc gaaaatgcg ccaagtctc 
      gacgaattg aaagacact gtggctttg gttttgtcc 
      aacggacat tacagtttt gaatctccg agacctttc 
      gtccctaat gtgattcca gtcggtggg tttcacgtc 
      caaaaagca cgaaaacta ccaactgat ctccagaaa 
      tatatggat gaggccaaa catggagtg gtttatttc 
      agtcttggg tctaacatg aaaagtgtc ttacttccg 
      aaagagaaa cagcaacaa attcttaaa gcgtttgcc 
      aaaattccg cacaaagtt ttgtggaaa tgggaggat 
      gataatttg gaaaacaaa ccagacaat gtcttgatc 
      cgcaagtgg tttccacaa aatgacatt ctaggccac 
      ccaaatttg aaattgttc ataacccat ggtggcctc 
      ttgagcacc attgaagcg ctacaccat ggggtccca 
      gtcctggga atcccaatt ttcggtgac cagaaagca 
      aacatcccc aacgccgtc tcctccgga tacgccgtc 
      cagttagag ttggccgat ttggacgaa gccacctta 
      accaaagcc ttggacgag atcttaacc aaccccaaa 
      taccaggaa aacgcgaaa aagcgctcc caactcctg 
      cacgaccag cccatgtcg cccatggac acggccgtt 
      ttctgggtg gagcacgtg atccggcac aaaggcgcc 
      ccgcatttg cgaaatctg ggcagttac ctgccctgg 
      taccagtac ctgatgctg gacgtggcg ctgtttgtg 
      gttttattt ttgctagtg gttgttggt attgtcggg 
      tttgttatt aggaaaatt tgcggttgt tgttgtcag 
      aggaaaagt aaggaaaaa agggatgca ttgagaggg 
      gcggcagcg gcggcgtcg cgacgcatg tctgggtgt 
      gatcctgtc tgtaccaaa cgacagtgt tcatcagtg 
      ctcaatatg tttacgttg tgtatgtcc cgagggcat 
      cctccctta caaaacgca caacaggaa aacaacgtt 
      gccaacaag caagtcaaa ggcggatac ctcctccga 
      tataaacga gggttattt tgtaacagc tggtgtgaa 
      gagtggttc gtgttgtac gaagactca actttggct 
      tggtttaaa gacaagggc ttgaaccgc ccaaagggt 
      cacctacgt attagcgaa tgcccggac ttactagcc 
      gtgggcgaa tggacacgt caagtgccc aaaagaccc 
      cgattcccg cgaaactac cacgtagga caagtccta 
      gccgttggc acaaggata ccccaggac gtgcactgg 
      cttatggca cagtcggca gccgaagcc aacgactgg 
      atgaccgca attagcaat actttgccg cctcctcca 
      aacttgccc ccagccaag agcaagagt ttcacgttc 
      cagaagaaa agcaacaat tgttacaaa tgcaattct 
      ctgaagaac gaggctgat tatacgctg atttcggga 
      gtggccatc gactggggc cacggctgg ggctggggg 
      cagacggcc tgggctgcc cacacggcc tgtgtctct 
      gagacggcg actgcactg gaggctgcc gtgaattcg 
      gcgctccat tgccatgcc ttgggggat tatggcgtt 
      ggaagtgga atggacgag ctcgactgt agtgcattt 
      ggggacttt attttctaa gcaggaatc aaaaatttg 
      tacctactt tgcagtcaa aacgtaaca attctaaac 
      tagggtact tatttcgcg gcgaaacgc tcaatattt 
      catagacaa ctgacctga attactcat ttcatgatt 
      tacttccgt ccagacaaa attaatttg cgtcgtaat 
      tatgaattc gtactccga ctatttcta aacatgtaa 
      ctgtcattc cttcacacg agcaataaa ctctcgcta 
      ggacattct aaaatgaac cgaacgttt ccattattc 
      atgaaataa gtgtaatgg tgaccgtca tatccataa 
      ctctctgaa tgtacgtta gattacaca aaaactcca 
      tgattaaag gtgctctaa ccgtaagat ttgcttata 
      gttgttgtt agaattgta aatgttttg tagatatac 
      atgaatgta taaggatat ttttgttag attagtact 
      ggaaactta gactgatgt aactatacc agaaatatt 
      tatacatgt gttgcgtct gctagggac gttcgtata 
      aatccagac tgccggtct aaaaattaa atgtcttag 
      cattgaaac gcacatagt taaagactg agtgcgaga 
      gaattaaat gttctaata agtatttaa aatgtaagc 
      caaactcca accctgcta gctagaggg agtaaatga 
      gagcataat acattcatc aagtggtca aaattttgt 
      actttagga taattggga ccaaacaac gttgtttat 
      ttcttcact caaatctct ttgtgtttc taaacagtg 
      aattaatta cttcgacaa tccacgtgc attcaaatg 
      ttctcatta tcgatgtca atacatgtg tgtaaaatg 
      caagtgtgt aatcattat tgtgatatc tgtataagt 
      agctattcc taatttaat aaataataa acaggtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY