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Gene information
General information
TC002100 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452015.1: 8975..14538
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002100
    iBeetle screen iB_06645

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002100 RA
      MKKPELICL LCVLQYTSA EIYAIRFDS QFYFDEFED 
      VPASFGKKL SDGVVRGVL VNADPPDGC TSALKPPPK 
      DVENETGRW IVLMPRYND VLNCSFEQK VRMGQGAGY 
      DAVVVHNVH SNQLVPMSA KNDTGINIP SVFVSESSG 
      STLKSIYAN PQYFIVITG ESPFNIQTH LLIPFAIVV 
      GICFIVMII FLVVKCIKD RRRQRRHRL PTSTLNKIP 
      ICKYQKGDP YETCAICLD DYIEGEKLR VLPCNHVYH 
      TKCIDPWLT KNRRVCPIC KRKVFAHDE PQHDSDSDS 
      DADDTTPLI NSNNRGTEG GTFQQQSEN PFRRAVRSI 
      SQQSGAVNF VTASDHHSI NGDYQSCNS STTESEESG 
      RVCDNLDEV HVHTQLEGS HSSGDVHV
    CDS sequence
    • >TC002100 RA
      atgaagaaa ccggagtta atctgcctg ctgtgcgtg 
      ctccagtac acctcggcc gaaatctac gctatacga 
      ttcgactcg cagttctac tttgacgaa ttcgaggat 
      gtccctgcc agttttggg aagaaattg tcagatgga 
      gtggttcgc ggcgtcttg gtgaacgcc gaccctcct 
      gacggctgc accagcgcc ctgaagccg ccccccaaa 
      gacgtggag aacgagacg gggcgatgg atcgtgctg 
      atgccgcgc tataacgac gttttgaat tgttctttc 
      gagcaaaag gtccgcatg ggtcagggg gctggttac 
      gacgccgtc gtggtccac aatgtccac tcgaaccag 
      ctggttcca atgtcggcg aaaaacgac accggaatt 
      aacattcct tcagtattt gtgagcgaa tcttccggc 
      tcgacgctg aaatcgatc tacgccaat ccgcaatat 
      ttcatcgtg atcaccggg gaatcaccg tttaatatt 
      cagacgcat ctcctgatc ccgtttgcg attgtcgtg 
      gggatttgt tttatcgta atgattatc tttctggtg 
      gtgaagtgt attaaagac aggaggagg cagaggaga 
      catcgcttg cccacgtcc acgcttaat aaaattccg 
      atttgtaaa taccaaaag ggggatccg tacgagacg 
      tgcgccatt tgtcttgat gactacatc gagggcgag 
      aagctcaga gttcttcca tgcaatcac gtttaccac 
      acgaaatgc atcgacccg tggctgacg aagaaccgg 
      cgcgtgtgc cccatttgc aagcgcaag gtgttcgcc 
      cacgacgag ccccagcac gactctgac agcgactcg 
      gacgccgac gacacgacc ccccttatc aactcgaat 
      aaccggggc accgaaggg ggcaccttc cagcaacag 
      agcgagaat ccgttccgt cgggccgtt cgctcaatt 
      tctcagcaa tcaggcgct gttaatttc gtcacagcc 
      tctgatcat cacagcatc aacggtgat tatcaaagt 
      tgtaactct tcgacgaca gaatctgag gaaagcgga 
      cgagtttgt gataattta gatgaagtt cacgttcac 
      acacagctc gagggcagt cacagtagt ggtgatgta 
      cacgtatga
    mRNA sequence
    • >TC002100 RA
      caaaagtgc tgctgttta ttttttgaa gttttttcc 
      cgattacgg gcgctaatc cggttaaca gtgatcgaa 
      tcgccccga ggccgatgc cccccggcg cccacccca 
      aagcccccg catgtcgct cgagtaaag tgcgttaat 
      tattttatt tacaaagac gtaatttga aggttgact 
      gtttgacat attaattaa gcatcctac aataacaat 
      atcggattg ctcattatc tccgtaaat tccctcatt 
      attatgata aaatcgagc caagagccc gtttagggg 
      tggctgttt tgaccaaat caaacttta cttagtgat 
      aaattatgt gatagttgg atttttata caaataaca 
      tgaagaaac cggagttaa tctgcctgc tgtgcgtgc 
      tccagtaca cctcggccg aaatctacg ctatacgat 
      tcgactcgc agttctact ttgacgaat tcgaggatg 
      tccctgcca gttttggga agaaattgt cagatggag 
      tggttcgcg gcgtcttgg tgaacgccg accctcctg 
      acggctgca ccagcgccc tgaagccgc cccccaaag 
      acgtggaga acgagacgg ggcgatgga tcgtgctga 
      tgccgcgct ataacgacg ttttgaatt gttctttcg 
      agcaaaagg tccgcatgg gtcaggggg ctggttacg 
      acgccgtcg tggtccaca atgtccact cgaaccagc 
      tggttccaa tgtcggcga aaaacgaca ccggaatta 
      acattcctt cagtatttg tgagcgaat cttccggct 
      cgacgctga aatcgatct acgccaatc cgcaatatt 
      tcatcgtga tcaccgggg aatcaccgt ttaatattc 
      agacgcatc tcctgatcc cgtttgcga ttgtcgtgg 
      ggatttgtt ttatcgtaa tgattatct ttctggtgg 
      tgaagtgta ttaaagaca ggaggaggc agaggagac 
      atcgcttgc ccacgtcca cgcttaata aaattccga 
      tttgtaaat accaaaagg gggatccgt acgagacgt 
      gcgccattt gtcttgatg actacatcg agggcgaga 
      agctcagag ttcttccat gcaatcacg tttaccaca 
      cgaaatgca tcgacccgt ggctgacga agaaccggc 
      gcgtgtgcc ccatttgca agcgcaagg tgttcgccc 
      acgacgagc cccagcacg actctgaca gcgactcgg 
      acgccgacg acacgaccc cccttatca actcgaata 
      accggggca ccgaagggg gcaccttcc agcaacaga 
      gcgagaatc cgttccgtc gggccgttc gctcaattt 
      ctcagcaat caggcgctg ttaatttcg tcacagcct 
      ctgatcatc acagcatca acggtgatt atcaaagtt 
      gtaactctt cgacgacag aatctgagg aaagcggac 
      gagtttgtg ataatttag atgaagttc acgttcaca 
      cacagctcg agggcagtc acagtagtg gtgatgtac 
      acgtatgag ttgtttctt tggctcttg tcgatttgt 
      ttaggttta gttgcttta acttctagt ctcggacga 
      gaaggttaa gagctggtg ctattatca gttgaatta 
      aatttattc tctgacttg tatttttat ttttaggtt 
      attgttttt tgatttgag gcgaggcac ggccttgtg 
      taagcttat acgaaatat atttttgat ggtcgaaat 
      tactttttg ttacggttg tgatttgaa ttactaaca 
      ctcgtaagt tttaaattt tatttgtat atttattgt 
      tttattctt atgccaaat aataatcta agtaagtgt 
      aacgagtct ctcaatctt gacaataac tcgtccctg 
      atgtacagg gtgcaccct ggaattctt aatctcacc 
      tgtatattt tgcaactag cttggcctt gctagctca 
      tgaatggga taacactgt tgttgagat tttcgtttt 
      ttcccctac tcttgtggc cctttttcc gggtttgta 
      cgaacccgc acaatattt tcagactca gataatagc 
      aaatctcga ttaggctta aaatgctga aaccgaggc 
      agaggccta agaggccgc caacgttcg ctgaaaaac 
      cctttcaga aaaaattgt tacgtgttt ggaaattat 
      tacataaaa cttcagtga agccgtaaa tttcgcttt 
      taatctgac gccgatacc ggttctctt tgaaaaaga 
      aatggagca cggccatgt ttttcctga aaaataatt 
      acactgaaa attgtggaa aatgttggt ttgcgatta 
      cacgaagta ttcgttagt gacgttctc caaacgctg 
      atttttgga catttgata caatttact gtaagttgc 
      gcttgtctc gaaattcat ttcgtaaca acttgccaa 
      acagttaca actcgatct ggaaataaa taaaaaaat 
      gtgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY