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Gene information
General information
TC033898 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 16461730..16482127
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC033898
      iBeetle screen iB_06634

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033898 RA
        MRVVAVCLF TILFFDGVL SQIGCKSSG 
        IRCSDPYTF QFCANFTNK ILTYGTATK 
        CPSSTICDN EGFFPCKVG TIPTTTPQP 
        TTVPACNGP AKFLTSVCN QYLECVFSF 
        LFWTPITNT CPAGQNFDP VLKECSEKY 
        SCGPDAITA EPPVIPPKC NGGGKLPGP 
        TPNQYYECE FIWFQFQPT LKSCFEGQM 
        FDVNVSLCV QMPETESVS TG
      CDS sequence
      • >TC033898 RA
        atgagagtt gtagcagtc tgtttgttc accatttta 
        ttctttgat ggtgtttta agccagatt ggatgcaag 
        tcctcaggg ataagatgt tcagaccct tacacgttt 
        cagttttgc gccaatttt acaaataag attctaaca 
        tacggtact gctacaaaa tgcccatct agtaccatt 
        tgcgataac gagggattt ttcccgtgc aaagttggt 
        accattccc acgaccacc cctcaacca acgacagtg 
        cctgcctgt aacggacct gcaaaattt cttacgtct 
        gtgtgtaat cagtacctt gaatgtgta tttagtttt 
        ctattttgg acacctatt accaacacg tgtcccgcg 
        gggcagaac tttgatcca gttcttaaa gagtgcagt 
        gagaagtat agttgtggg ccagatgcc attacagcg 
        gaaccacca gtaatacca cctaaatgt aacggtggt 
        ggaaaattg ccaggacca actccaaac cagtactac 
        gagtgtgaa ttcatttgg ttccagttc caaccaaca 
        ctaaaatcg tgttttgaa ggacaaatg tttgatgtt 
        aatgttagt ttatgcgtc cagatgcca gaaacagag 
        tctgtgtcg actggataa
      mRNA sequence
      • >TC033898 RA
        ggcaatgaa acaaaacaa atatttgta cacaagtaa 
        gacaaaagg taaaaacgc gaaacaact atttacata 
        tttgtgaac atttctaac atccaaaat gagagttgt 
        agcagtctg tttgttcac cattttatt ctttgatgg 
        tgttttaag ccagattgg atgcaagtc ctcagggat 
        aagatgttc agaccctta cacgtttca gttttgcgc 
        caattttac aaataagat tctaacata cggtactgc 
        tacaaaatg cccatctag taccatttg cgataacga 
        gggattttt cccgtgcaa agttggtac cattcccac 
        gaccacccc tcaaccaac gacagtgcc tgcctgtaa 
        cggacctgc aaaatttct tacgtctgt gtgtaatca 
        gtaccttga atgtgtatt tagttttct attttggac 
        acctattac caacacgtg tcccgcggg gcagaactt 
        tgatccagt tcttaaaga gtgcagtga gaagtatag 
        ttgtgggcc agatgccat tacagcgga accaccagt 
        aataccacc taaatgtaa cggtggtgg aaaattgcc 
        aggaccaac tccaaacca gtactacga gtgtgaatt 
        catttggtt ccagttcca accaacact aaaatcgtg 
        ttttgaagg acaaatgtt tgatgttaa tgttagttt 
        atgcgtcca gatgccaga aacagagtc tgtgtcgac 
        tggataaag cataaagtg agttaagct gaatttcgc 
        caattcttt gttcaagtt atttattaa taaagaatt 
        atgattaaa aattgtacg tttattttt attttttta 
        ataaagcgc ggtttttgt gcagttgtg cacttgcct 
        tgacttata tcgattggt tttgtgtca caaaatcat 
        gtctcttcg taggataac aacctcgac tacgaatgt 
        aaccaattt gaaccgaag aacgtgatc tcgttgaaa 
        tcgatcgaa ttttttatc ggccctggg gaatgaatg 
        tatctgaag acgaaatag tccacctcg aaactgttg 
        aatctccaa caaaatact ctcttaatt tcaaatttg 
        atgaattag tcccagctg ctattaaat acgaccctt 
        taatgactg taattggta cgaataaaa gtattgcaa 
        taat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY