Status
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Gene information
General information
TC031257
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014822,TC014823
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452040.1: 6845..15937
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031257
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06633

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031257 RA
      MNSPIRKPS ASLCTEDMM TCLYNNLLA YVWHRMAKH 
      IFKCHREED AKRKRESEN KQNVEDQTE SWDNENYLN 
      KNKQNAEDQ EESWDNENY LTENKQNVN DQDESWDND 
      DYLTENKQN SENEEECWD NESYLICMQ FIRLPHMDE 
      DYVIGEEDC LYLTVYTAH LNASFDVVV YIHGGDFAT 
      GYASVYEPD YFIDKNIVF VNLNYRLGP LGWFLSTED 
      DVVPGNNGL KDQILALKF IKKYIRHFG GNPDSITLF 
      GDGTSVNFH YLSPQSRGL FHRGWSMSG TMLVPWALM 
      EQPLAQTKK LATVVGCGT DGTMIKCLK SRPARQITV 
      TIPEYQAWW YLPFAQFAP VVDSWATQP VLSAHPYQM 
      IKNQQVYDV LWIVSFTNS EGLYAINEI YDNALLALL 
      DDYWNEYIP PILYYNYTV DRDLQDEVS QKIRKYYLG 
      EKKLSKRTF RELVAMFSD RIFINDIPT YALKFEYRS 
      GNSWFGLET ETGRNFGAA HADDLLYIF KRDPFDTSG 
      SQRDRAMVK IFVDLLASY SHTRIPKVK TMEWLQVPK 
      NATDDLTFL KIGAPNDVT VETSVQGSA PFWDSLPIK 
      ENEKIP
    CDS sequence
    • >TC031257 RA
      atgaatagt ccaataaga aaaccatca gccagcttg 
      tgcactgag gatatgatg acatgtctc tataataac 
      ctactagcc tatgtatgg cacaggatg gccaaacac 
      atttttaag tgtcacaga gaagaggac gcaaaaaga 
      aaaagagag tccgaaaat aaacaaaac gttgaagat 
      cagacagaa agttgggat aacgaaaat tatcttaat 
      aaaaataaa caaaacgcc gaagatcag gaagaaagt 
      tgggataac gaaaattat ctaactgaa aataaacaa 
      aacgttaac gatcaggat gaaagttgg gataatgac 
      gattatctg actgaaaat aaacaaaat tccgaaaat 
      gaagaggaa tgttgggat aacgaaagt tatcttata 
      tgtatgcag tttatcaga ctcccacat atggacgaa 
      gactatgtg attggtgag gaggattgt ctttatttg 
      accgtttac acggcccac ttgaatgct agttttgat 
      gtggttgtg tacattcat ggtggggat ttcgcgact 
      ggttatgcc agtgtttac gaacctgac tattttata 
      gacaaaaat atagttttc gtaaacttg aattataga 
      ttagggccg ttaggttgg tttttgagt actgaggat 
      gacgtagta cctggcaat aatgggctc aaagaccaa 
      atcctggcc ttaaaattc atcaaaaaa tatatccgc 
      cattttggt ggtaatcct gactcgatc acacttttc 
      ggagatggt actagtgtt aacttccat tacttatcg 
      ccacaatcc cgtgggttg ttccaccgt ggttggtcc 
      atgagtggt accatgcta gtaccatgg gccctaatg 
      gagcaacca ttggcccaa acgaaaaaa ctcgccact 
      gtcgtgggt tgtggtacc gatgggaca atgatcaag 
      tgtttgaaa agtagacca gccaggcaa attacggtc 
      acaatacca gagtaccaa gcttggtgg tacctgcca 
      tttgcccaa tttgcgccc gtggttgac tcctgggcc 
      actcaaccg gtactatca gcccatcct taccaaatg 
      atcaaaaat caacaagtc tatgacgtt ctttggatc 
      gtttctttt acaaactct gaaggcttg tacgcaatt 
      aacgagatc tacgataat gcacttttg gcacttctt 
      gatgactac tggaatgag tatattcca ccaattttg 
      tactacaat tacaccgtg gatcgagat ttgcaagat 
      gaggtgagt cagaagatt aggaagtac taccttgga 
      gagaagaaa ttgtcgaag aggacgttt cgggagttg 
      gtggcgatg ttctcagac cggattttt atcaacgat 
      atccccacc tatgccttg aaatttgaa tacaggagt 
      gggaatagt tggttcggg cttgaaaca gagactggg 
      aggaatttt ggagcagct catgctgat gacctcctc 
      tatattttc aagagggat ccatttgat acaagcgga 
      agccaacga gatagagct atggttaaa atttttgtc 
      gatttgttg gcatcgtat agtcacacg cgcattcca 
      aaagtaaaa acaatggag tggctacaa gtaccaaaa 
      aatgccaca gatgacttg actttccta aaaattgga 
      gccccgaac gatgtgact gtggaaacg tcagtccaa 
      ggcagtgcc ccattttgg gattcgcta ccaatcaag 
      gaaaatgaa aagattcca taa
    mRNA sequence
    • >TC031257 RA
      cctttaaaa agatgaata gtccaataa gaaaaccat 
      cagccagct tgtgcactg aggatatga tgacatgtc 
      tctataata acctactag cctatgtat ggcacagga 
      tggccaaac acattttta agtgtcaca gagaagagg 
      acgcaaaaa gaaaaagag agtccgaaa ataaacaaa 
      acgttgaag atcagacag aaagttggg ataacgaaa 
      attatctta ataaaaata aacaaaacg ccgaagatc 
      aggaagaaa gttgggata acgaaaatt atctaactg 
      aaaataaac aaaacgtta acgatcagg atgaaagtt 
      gggataatg acgattatc tgactgaaa ataaacaaa 
      attccgaaa atgaagagg aatgttggg ataacgaaa 
      gttatctta tatgtatgc agtttatca gactcccac 
      atatggacg aagactatg tgattggtg aggaggatt 
      gtctttatt tgaccgttt acacggccc acttgaatg 
      ctagttttg atgtggttg tgtacattc atggtgggg 
      atttcgcga ctggttatg ccagtgttt acgaacctg 
      actatttta tagacaaaa atatagttt tcgtaaact 
      tgaattata gattagggc cgttaggtt ggtttttga 
      gtactgagg atgacgtag tacctggca ataatgggc 
      tcaaagacc aaatcctgg ccttaaaat tcatcaaaa 
      aatatatcc gccattttg gtggtaatc ctgactcga 
      tcacacttt tcggagatg gtactagtg ttaacttcc 
      attacttat cgccacaat cccgtgggt tgttccacc 
      gtggttggt ccatgagtg gtaccatgc tagtaccat 
      gggccctaa tggagcaac cattggccc aaacgaaaa 
      aactcgcca ctgtcgtgg gttgtggta ccgatggga 
      caatgatca agtgtttga aaagtagac cagccaggc 
      aaattacgg tcacaatac cagagtacc aagcttggt 
      ggtacctgc catttgccc aatttgcgc ccgtggttg 
      actcctggg ccactcaac cggtactat cagcccatc 
      cttaccaaa tgatcaaaa atcaacaag tctatgacg 
      ttctttgga tcgtttctt ttacaaact ctgaaggct 
      tgtacgcaa ttaacgaga tctacgata atgcacttt 
      tggcacttc ttgatgact actggaatg agtatattc 
      caccaattt tgtactaca attacaccg tggatcgag 
      atttgcaag atgaggtga gtcagaaga ttaggaagt 
      actaccttg gagagaaga aattgtcga agaggacgt 
      ttcgggagt tggtggcga tgttctcag accggattt 
      ttatcaacg atatcccca cctatgcct tgaaatttg 
      aatacagga gtgggaata gttggttcg ggcttgaaa 
      cagagactg ggaggaatt ttggagcag ctcatgctg 
      atgacctcc tctatattt tcaagaggg atccatttg 
      atacaagcg gaagccaac gagatagag ctatggtta 
      aaatttttg tcgatttgt tggcatcgt atagtcaca 
      cgcgcattc caaaagtaa aaacaatgg agtggctac 
      aagtaccaa aaaatgcca cagatgact tgactttcc 
      taaaaattg gagccccga acgatgtga ctgtggaaa 
      cgtcagtcc aaggcagtg ccccatttt gggattcgc 
      taccaatca aggaaaatg aaaagattc cataatttt 
      gaaataaat tgtacatca gttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY