General information | |||
TC031257
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015452040.1: 6845..15937 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC031257 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06633 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
head number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031257 RA
MNSPIRKPS ASLCTEDMM TCLYNNLLA YVWHRMAKH IFKCHREED AKRKRESEN KQNVEDQTE SWDNENYLN KNKQNAEDQ EESWDNENY LTENKQNVN DQDESWDND DYLTENKQN SENEEECWD NESYLICMQ FIRLPHMDE DYVIGEEDC LYLTVYTAH LNASFDVVV YIHGGDFAT GYASVYEPD YFIDKNIVF VNLNYRLGP LGWFLSTED DVVPGNNGL KDQILALKF IKKYIRHFG GNPDSITLF GDGTSVNFH YLSPQSRGL FHRGWSMSG TMLVPWALM EQPLAQTKK LATVVGCGT DGTMIKCLK SRPARQITV TIPEYQAWW YLPFAQFAP VVDSWATQP VLSAHPYQM IKNQQVYDV LWIVSFTNS EGLYAINEI YDNALLALL DDYWNEYIP PILYYNYTV DRDLQDEVS QKIRKYYLG EKKLSKRTF RELVAMFSD RIFINDIPT YALKFEYRS GNSWFGLET ETGRNFGAA HADDLLYIF KRDPFDTSG SQRDRAMVK IFVDLLASY SHTRIPKVK TMEWLQVPK NATDDLTFL KIGAPNDVT VETSVQGSA PFWDSLPIK ENEKIP
- >TC031257 RA
atgaatagt ccaataaga aaaccatca gccagcttg tgcactgag gatatgatg acatgtctc tataataac ctactagcc tatgtatgg cacaggatg gccaaacac atttttaag tgtcacaga gaagaggac gcaaaaaga aaaagagag tccgaaaat aaacaaaac gttgaagat cagacagaa agttgggat aacgaaaat tatcttaat aaaaataaa caaaacgcc gaagatcag gaagaaagt tgggataac gaaaattat ctaactgaa aataaacaa aacgttaac gatcaggat gaaagttgg gataatgac gattatctg actgaaaat aaacaaaat tccgaaaat gaagaggaa tgttgggat aacgaaagt tatcttata tgtatgcag tttatcaga ctcccacat atggacgaa gactatgtg attggtgag gaggattgt ctttatttg accgtttac acggcccac ttgaatgct agttttgat gtggttgtg tacattcat ggtggggat ttcgcgact ggttatgcc agtgtttac gaacctgac tattttata gacaaaaat atagttttc gtaaacttg aattataga ttagggccg ttaggttgg tttttgagt actgaggat gacgtagta cctggcaat aatgggctc aaagaccaa atcctggcc ttaaaattc atcaaaaaa tatatccgc cattttggt ggtaatcct gactcgatc acacttttc ggagatggt actagtgtt aacttccat tacttatcg ccacaatcc cgtgggttg ttccaccgt ggttggtcc atgagtggt accatgcta gtaccatgg gccctaatg gagcaacca ttggcccaa acgaaaaaa ctcgccact gtcgtgggt tgtggtacc gatgggaca atgatcaag tgtttgaaa agtagacca gccaggcaa attacggtc acaatacca gagtaccaa gcttggtgg tacctgcca tttgcccaa tttgcgccc gtggttgac tcctgggcc actcaaccg gtactatca gcccatcct taccaaatg atcaaaaat caacaagtc tatgacgtt ctttggatc gtttctttt acaaactct gaaggcttg tacgcaatt aacgagatc tacgataat gcacttttg gcacttctt gatgactac tggaatgag tatattcca ccaattttg tactacaat tacaccgtg gatcgagat ttgcaagat gaggtgagt cagaagatt aggaagtac taccttgga gagaagaaa ttgtcgaag aggacgttt cgggagttg gtggcgatg ttctcagac cggattttt atcaacgat atccccacc tatgccttg aaatttgaa tacaggagt gggaatagt tggttcggg cttgaaaca gagactggg aggaatttt ggagcagct catgctgat gacctcctc tatattttc aagagggat ccatttgat acaagcgga agccaacga gatagagct atggttaaa atttttgtc gatttgttg gcatcgtat agtcacacg cgcattcca aaagtaaaa acaatggag tggctacaa gtaccaaaa aatgccaca gatgacttg actttccta aaaattgga gccccgaac gatgtgact gtggaaacg tcagtccaa ggcagtgcc ccattttgg gattcgcta ccaatcaag gaaaatgaa aagattcca taa
- >TC031257 RA
cctttaaaa agatgaata gtccaataa gaaaaccat cagccagct tgtgcactg aggatatga tgacatgtc tctataata acctactag cctatgtat ggcacagga tggccaaac acattttta agtgtcaca gagaagagg acgcaaaaa gaaaaagag agtccgaaa ataaacaaa acgttgaag atcagacag aaagttggg ataacgaaa attatctta ataaaaata aacaaaacg ccgaagatc aggaagaaa gttgggata acgaaaatt atctaactg aaaataaac aaaacgtta acgatcagg atgaaagtt gggataatg acgattatc tgactgaaa ataaacaaa attccgaaa atgaagagg aatgttggg ataacgaaa gttatctta tatgtatgc agtttatca gactcccac atatggacg aagactatg tgattggtg aggaggatt gtctttatt tgaccgttt acacggccc acttgaatg ctagttttg atgtggttg tgtacattc atggtgggg atttcgcga ctggttatg ccagtgttt acgaacctg actatttta tagacaaaa atatagttt tcgtaaact tgaattata gattagggc cgttaggtt ggtttttga gtactgagg atgacgtag tacctggca ataatgggc tcaaagacc aaatcctgg ccttaaaat tcatcaaaa aatatatcc gccattttg gtggtaatc ctgactcga tcacacttt tcggagatg gtactagtg ttaacttcc attacttat cgccacaat cccgtgggt tgttccacc gtggttggt ccatgagtg gtaccatgc tagtaccat gggccctaa tggagcaac cattggccc aaacgaaaa aactcgcca ctgtcgtgg gttgtggta ccgatggga caatgatca agtgtttga aaagtagac cagccaggc aaattacgg tcacaatac cagagtacc aagcttggt ggtacctgc catttgccc aatttgcgc ccgtggttg actcctggg ccactcaac cggtactat cagcccatc cttaccaaa tgatcaaaa atcaacaag tctatgacg ttctttgga tcgtttctt ttacaaact ctgaaggct tgtacgcaa ttaacgaga tctacgata atgcacttt tggcacttc ttgatgact actggaatg agtatattc caccaattt tgtactaca attacaccg tggatcgag atttgcaag atgaggtga gtcagaaga ttaggaagt actaccttg gagagaaga aattgtcga agaggacgt ttcgggagt tggtggcga tgttctcag accggattt ttatcaacg atatcccca cctatgcct tgaaatttg aatacagga gtgggaata gttggttcg ggcttgaaa cagagactg ggaggaatt ttggagcag ctcatgctg atgacctcc tctatattt tcaagaggg atccatttg atacaagcg gaagccaac gagatagag ctatggtta aaatttttg tcgatttgt tggcatcgt atagtcaca cgcgcattc caaaagtaa aaacaatgg agtggctac aagtaccaa aaaatgcca cagatgact tgactttcc taaaaattg gagccccga acgatgtga ctgtggaaa cgtcagtcc aaggcagtg ccccatttt gggattcgc taccaatca aggaaaatg aaaagattc cataatttt gaaataaat tgtacatca gttt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
