Status
Please wait ...
Gene information
General information
TC031583
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001686,TC001685
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7527077..7554045
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031583
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
        ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
        {{goTerm.showAll ? '[-]' : '[+]'}}
        Status: {{ goTerm.status }}
        Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
        Reference: {{ goTerm.referenceText }}
        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_06632

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031583 RA
        METKTRTRK LLTADLKKA VIRCFRELE EAYSHLVKK 
        PNVYEKVAY IFATILFEL KANMLTTPL SEEVLLDEL 
        LRHFPERIQ SLWILEKNC TTTEAAEFL AAQEIPGQN 
        PGEKTTIAP RESPRTSDH QLRKDTKRP RPNKDRNEV 
        TRTEAPENR GNRFQNRTS GNQQWRNNK ANFSNSRWY 
        NNTTSSNNH WRKNNPNST NNNTNSRAK GETSRNPKD 
        SGNGAEYRA GPKVLRSAT RPTKRQQGS TIENRRKTK 
        TLDFVSLYG RKKRCSTAS ITGNNNKQK IVPEIEIQI 
        SQQQSVPAL LNTGSEVSC ISEEVWAKL IETGNKPPA 
        PTKKVGKKH QNLKDGFKK ARAEIC
      • >TC031583 RB
        METKTRTRK LLTADLKKA VIRCFRELE EAYSHLVKK 
        PNVYEKVAY IFATILFEL KANMLTTPL SEEVLLDEL 
        LRHFPERIQ SLWILEKNC TTTEAAEFL AAQEIPGQN 
        PGEKTTIAP RESPRTSDH QLRKDTKRP RPNKDRNEV 
        TRTEAPENR GNRFQNRTS GNQQWRNNK ANFSNSRWY 
        NNTTSSNNH WRKNNPNST NNNTNSRAK GETSRNPKD 
        SGNGAEYRA GPKVLRSAT RPTKRQQGS TIENRRKTK 
        TLDFVSLYG RKKRCSTAS ITGNNNKQK IVPEIEIQI 
        SQQQSVPAL LNTGSEVSC ISEEVWAKL IETGNKPPA 
        LP
      CDS sequence
      • >TC031583 RA
        atggaaacc aaaactcga acccgaaaa ctactgacc 
        gctgatttg aaaaaagcg gtcataagg tgtttccgg 
        gaattggag gaggcttac agccatttg gtaaaaaag 
        ccaaatgtg tacgaaaaa gtggcctat atttttgcg 
        acgatattg ttcgaactc aaggcgaac atgttgaca 
        acaccttta tcggaggaa gtgctgttg gacgaattg 
        cttcgccac ttccctgaa agaatccaa tcgctttgg 
        atactagaa aagaattgt accaccacg gaggccgcg 
        gaatttctg gcagcacaa gaaattccg ggacaaaat 
        ccaggagag aaaactacc atcgctccc agggaaagt 
        ccccgcact tctgaccac cagctgcgt aaagacaca 
        aagcgtccg cggccaaac aaggatcgc aacgaagta 
        actcgtact gaagccccg gaaaatcgc ggaaatcgg 
        tttcaaaac cgcacaagc ggcaaccag cagtggcgc 
        aacaacaaa gccaacttt agcaacagc cgctggtac 
        aacaacacc actagcagc aacaatcac tggcgcaag 
        aacaacccc aactccacc aacaataac acgaattcg 
        agagcgaag ggtgaaaca tcacgaaac cctaaagat 
        tcgggaaac ggggcggag tacagagcg gggcctaag 
        gtactccgg tcagcgaca cggcccaca aagcgacag 
        cagggatca acaattgag aataggagg aaaacgaaa 
        acgttagac ttcgtctca ctctatgga aggaaaaaa 
        cgatgctcg accgcgagc atcacaggt aacaacaac 
        aaacagaaa atcgtgccg gagattgag atccaaatc 
        tcccagcaa caatcagtc cccgcactt ttgaacacc 
        ggcagcgaa gtcagctgt atctccgaa gaagtttgg 
        gccaagctt attgagacc ggaaacaaa ccccccgcg 
        cctacaaag aaagttggg aaaaaacac cagaattta 
        aaggatggc ttcaaaaaa gccagggcc gaaatttgt 
        taa
      • >TC031583 RB
        atggaaacc aaaactcga acccgaaaa ctactgacc 
        gctgatttg aaaaaagcg gtcataagg tgtttccgg 
        gaattggag gaggcttac agccatttg gtaaaaaag 
        ccaaatgtg tacgaaaaa gtggcctat atttttgcg 
        acgatattg ttcgaactc aaggcgaac atgttgaca 
        acaccttta tcggaggaa gtgctgttg gacgaattg 
        cttcgccac ttccctgaa agaatccaa tcgctttgg 
        atactagaa aagaattgt accaccacg gaggccgcg 
        gaatttctg gcagcacaa gaaattccg ggacaaaat 
        ccaggagag aaaactacc atcgctccc agggaaagt 
        ccccgcact tctgaccac cagctgcgt aaagacaca 
        aagcgtccg cggccaaac aaggatcgc aacgaagta 
        actcgtact gaagccccg gaaaatcgc ggaaatcgg 
        tttcaaaac cgcacaagc ggcaaccag cagtggcgc 
        aacaacaaa gccaacttt agcaacagc cgctggtac 
        aacaacacc actagcagc aacaatcac tggcgcaag 
        aacaacccc aactccacc aacaataac acgaattcg 
        agagcgaag ggtgaaaca tcacgaaac cctaaagat 
        tcgggaaac ggggcggag tacagagcg gggcctaag 
        gtactccgg tcagcgaca cggcccaca aagcgacag 
        cagggatca acaattgag aataggagg aaaacgaaa 
        acgttagac ttcgtctca ctctatgga aggaaaaaa 
        cgatgctcg accgcgagc atcacaggt aacaacaac 
        aaacagaaa atcgtgccg gagattgag atccaaatc 
        tcccagcaa caatcagtc cccgcactt ttgaacacc 
        ggcagcgaa gtcagctgt atctccgaa gaagtttgg 
        gccaagctt attgagacc ggaaacaaa ccccccgcg 
        ttaccataa
      mRNA sequence
      • >TC031583 RA
        taacgaaag tgcaattac tttgatatt ttttgtttt 
        ttttatgtg taaggttgt tttttgttt ttgtataaa 
        atcgaaact aaactagat caggatatt tggacggaa 
        tatccgagt gggccggtt cgcagcagg aatgcttaa 
        ggtcatgtc caagtttgg cggggaaat gttgataat 
        cctctgcaa ccttatgta tgccgtatc tacgctttc 
        acgctcgcc gacatttgc gagtggctg atgagcaca 
        gtgagcata acgccaggt ttaaaagtc ttcttttct 
        attgaaccc tttgattct tttccaaaa taccggctc 
        ccaacgcga gcactatga gcggcgagg tgaataagg 
        agagcatct gcatcccga ttggtatcg ccactcgtg 
        cgaatgtgt agatgcgac accatttgt ttctccgtg 
        ttagttgtg gttgtgtct ttctgggac accttctca 
        ttgttattt cattctaac tactctgga gtctattat 
        caccgggaa tatggagag tacaagatg cctcaagca 
        tgaagataa ctagaaaac tagaaccct atagcaatc 
        gatttttgt ttgaggcaa atacggtat tatgccatg 
        ttgaatgtt ttttcaacg tgattaact agagaggcc 
        ctacttatt ggttctatc gtgaatata ttgtaaggg 
        gcagttgct tccatttca cttagaatc attccatcc 
        aaatattag ttgtaatgt gagtattta aacaagtag 
        taagagttc cttttacta attaaaaat ttaaattcg 
        tctttttaa tgaatgtat ttgtaagat gtagtgatt 
        atgtgtttt tttttgtaa aataaatag agaatataa 
        aatttgtca attggtgtt tcctttatc ttctacaaa 
        gaagttttt ttttcttga ctgataatt tatacctat 
        cagattagt gttttggtt gattgtgtt tgtaaaatc 
        cgttaaaat ggaaaccaa aactcgaac ccgaaaact 
        actgaccgc tgatttgaa aaaagcggt cataaggtg 
        tttccggga attggagga ggcttacag ccatttggt 
        aaaaaagcc aaatgtgta cgaaaaagt ggcctatat 
        ttttgcgac gatattgtt cgaactcaa ggcgaacat 
        gttgacaac acctttatc ggaggaagt gctgttgga 
        cgaattgct tcgccactt ccctgaaag aatccaatc 
        gctttggat actagaaaa gaattgtac caccacgga 
        ggccgcgga atttctggc agcacaaga aattccggg 
        acaaaatcc aggagagaa aactaccat cgctcccag 
        ggaaagtcc ccgcacttc tgaccacca gctgcgtaa 
        agacacaaa gcgtccgcg gccaaacaa ggatcgcaa 
        cgaagtaac tcgtactga agccccgga aaatcgcgg 
        aaatcggtt tcaaaaccg cacaagcgg caaccagca 
        gtggcgcaa caacaaagc caactttag caacagccg 
        ctggtacaa caacaccac tagcagcaa caatcactg 
        gcgcaagaa caaccccaa ctccaccaa caataacac 
        gaattcgag agcgaaggg tgaaacatc acgaaaccc 
        taaagattc gggaaacgg ggcggagta cagagcggg 
        gcctaaggt actccggtc agcgacacg gcccacaaa 
        gcgacagca gggatcaac aattgagaa taggaggaa 
        aacgaaaac gttagactt cgtctcact ctatggaag 
        gaaaaaacg atgctcgac cgcgagcat cacaggtaa 
        caacaacaa acagaaaat cgtgccgga gattgagat 
        ccaaatctc ccagcaaca atcagtccc cgcactttt 
        gaacaccgg cagcgaagt cagctgtat ctccgaaga 
        agtttgggc caagcttat tgagaccgg aaacaaacc 
        ccccgcgcc tacaaagaa agttgggaa aaaacacca 
        gaatttaaa ggatggctt caaaaaagc cagggccga 
        aatttgtta aatgcaagc cttgtgata aaaacataa 
        ttatcacca gtggcaaag atgctttaa ttaagcaca 
        gtgctgagc atcaaaaca aagtcaaag caatagcaa 
        aacaaccta cttcgtcat cgtttctgg cccaaggta 
        cctcaagca aattgttag agcaagata ttaaaaaag 
        gtgggtgtt gtattgtaa caattgagt tttataaaa 
        aacgacttt tcttattta gacgaaata cgtttggct 
        ggtttcatc gcagaacat aatttgcca ttttctttg 
        atggaccac ctcccaaaa gtgattgct gccgtatgc 
        catgactcc aaaattgca aagggttta aattgtgga 
        agaacaaaa actacaaaa gttgttgaa aatgttttg 
        ggccgtgaa cgactgagt t
      • >TC031583 RB
        taacgaaag tgcaattac tttgatatt ttttgtttt 
        ttttatgtg taaggttgt tttttgttt ttgtataaa 
        atcgaaact aaactagat caggatatt tggacggaa 
        tatccgagt gggccggtt cgcagcagg aatgcttaa 
        ggtcatgtc caagtttgg cggggaaat gttgataat 
        cctctgcaa ccttatgta tgccgtatc tacgctttc 
        acgctcgcc gacatttgc gagtggctg atgagcaca 
        gtgagcata acgccaggt ttaaaagtc ttcttttct 
        attgaaccc tttgattct tttccaaaa taccggctc 
        ccaacgcga gcactatga gcggcgagg tgaataagg 
        agagcatct gcatcccga ttggtatcg ccactcgtg 
        cgaatgtgt agatgcgac accatttgt ttctccgtg 
        ttagttgtg gttgtgtct ttctgggac accttctca 
        ttgttattt cattctaac tactctgga gtctattat 
        caccgggaa tatggagag tacaagatg cctcaagca 
        tgaagataa ctagaaaac tagaaccct atagcaatc 
        gatttttgt ttgaggcaa atacggtat tatgccatg 
        ttgaatgtt ttttcaacg tgattaact agagaggcc 
        ctacttatt ggttctatc gtgaatata ttgtaaggg 
        gcagttgct tccatttca cttagaatc attccatcc 
        aaatattag ttgtaatgt gagtattta aacaagtag 
        taagagttc cttttacta attaaaaat ttaaattcg 
        tctttttaa tgaatgtat ttgtaagat gtagtgatt 
        atgtgtttt tttttgtaa aataaatag agaatataa 
        aatttgtca attggtgtt tcctttatc ttctacaaa 
        gaagttttt ttttcttga ctgataatt tatacctat 
        cagattagt gttttggtt gattgtgtt tgtaaaatc 
        cgttaaaat ggaaaccaa aactcgaac ccgaaaact 
        actgaccgc tgatttgaa aaaagcggt cataaggtg 
        tttccggga attggagga ggcttacag ccatttggt 
        aaaaaagcc aaatgtgta cgaaaaagt ggcctatat 
        ttttgcgac gatattgtt cgaactcaa ggcgaacat 
        gttgacaac acctttatc ggaggaagt gctgttgga 
        cgaattgct tcgccactt ccctgaaag aatccaatc 
        gctttggat actagaaaa gaattgtac caccacgga 
        ggccgcgga atttctggc agcacaaga aattccggg 
        acaaaatcc aggagagaa aactaccat cgctcccag 
        ggaaagtcc ccgcacttc tgaccacca gctgcgtaa 
        agacacaaa gcgtccgcg gccaaacaa ggatcgcaa 
        cgaagtaac tcgtactga agccccgga aaatcgcgg 
        aaatcggtt tcaaaaccg cacaagcgg caaccagca 
        gtggcgcaa caacaaagc caactttag caacagccg 
        ctggtacaa caacaccac tagcagcaa caatcactg 
        gcgcaagaa caaccccaa ctccaccaa caataacac 
        gaattcgag agcgaaggg tgaaacatc acgaaaccc 
        taaagattc gggaaacgg ggcggagta cagagcggg 
        gcctaaggt actccggtc agcgacacg gcccacaaa 
        gcgacagca gggatcaac aattgagaa taggaggaa 
        aacgaaaac gttagactt cgtctcact ctatggaag 
        gaaaaaacg atgctcgac cgcgagcat cacaggtaa 
        caacaacaa acagaaaat cgtgccgga gattgagat 
        ccaaatctc ccagcaaca atcagtccc cgcactttt 
        gaacaccgg cagcgaagt cagctgtat ctccgaaga 
        agtttgggc caagcttat tgagaccgg aaacaaacc 
        ccccgcgtt accataact cataaccat caatacgaa 
        gacatcgaa tgccagaag atagagaat atttacatc 
        gccgaaaca gaatcgtac caaacaatg aagattgca 
        taattgaaa taattcaaa atacaagat ccaacagat 
        g
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY