General information | |||
TC001694 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 7718938..7722234 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC001694 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06631 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03234 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head appendages random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdominal segment 1 constricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Comment: Constriction often affecting A1 or T3/-A2 |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001694 RA
MSNESAELT KSWCLVDSS GVQEVTENE THDSDTDSI SVISESGCS YRTDEDEVA TPFEEPQLS EEAPVGEPE IVEEPPPLD EVKSIEKFL YLATSAFCA VIIIGFSVA VQQYLYNSS DVDNYEPDF AFPRVQNIK TDPPKKYPP KRKSHIKLD ELENKFAKL EVDNERHDK IAPAGGDND PRAEGNLKS NYKLNKNKS PVKCNKENC DAVRNDQEN RNDETYDKK IKELKVKED YLREKEKFL IEKEHDLLK KEIKLNREK ESYDNTKND KKRHYKEDD AKKPKDRKK KQKLAEEPY RKRNLSGQW YISMHEARD NMRQKKRSL FKKNKAHWY FQWMVDRAR LRVK
- >TC001694 RA
atgagcaac gagagtgcc gaactgacg aagtcatgg tgccttgtg gactcctca ggagtgcag gaagttacc gagaatgaa acgcacgat tccgacaca gacagtatt agcgttatc agtgaaagt ggctgttcg tacaggaca gacgaggat gaagtagca acccctttt gaagaaccc caattaagc gaggaagct ccagttggt gagcctgaa attgtggaa gaaccaccg ccgctagac gaggtcaaa agcatcgaa aaattcctc tatctcgcc acgagcgcc ttttgcgct gttataata atcgggttc tcggttgcg gtgcaacaa tacctttac aattcttct gatgtagat aattatgaa ccggatttc gcgttccct cgtgtacaa aatatcaaa acagaccct ccaaaaaag taccccccg aagcggaaa agtcacatc aaactagac gaattagaa aataagttt gctaaatta gaagtagac aacgaaagg catgacaag atcgcaccg gctggtgga gacaatgac ccccgagcc gagggaaat ctcaaatct aattataaa cttaacaaa aataaaagc ccagtcaaa tgtaacaaa gaaaattgc gacgcagta cgaaacgac caagaaaat cgaaatgac gagacctac gacaaaaaa atcaaggaa ctgaaagtg aaggaggat tatctcaga gagaaggaa aagtttctc attgagaaa gaacacgac ttgttgaag aaggagatc aagcttaac agggagaag gagagctac gacaatacc aaaaatgac aagaaaaga cattataag gaggatgat gctaaaaag cccaaagac cgcaagaag aaacaaaaa ctggccgag gagccgtat agaaaacgc aacctttcg ggccagtgg tatataagc atgcacgaa gctagggac aacatgcgg cagaaaaaa cggtcgctg tttaaaaaa aacaaggct cattggtac ttccagtgg atggtggat agagcaagg cttcgcgtc aagtga
- >TC001694 RA
agctgacaa tgacaggga atgaaaata aacaaatag taaaaacgc aatttcatt gttgatgtt cccagaagt gcagaatga actgatttg cacgtaaaa catcacgga cgggtgcag agttgcgag ttggccacc ttacatgag tcagggaag tgcagcacg cggcatttt gcacaaaga aagctgaca tattgttgc gtcgttaga caatgagca acgagagtg ccgaactga cgaagtcat ggtgccttg tggactcct caggagtgc aggaagtta ccgagaatg aaacgcacg attccgaca cagacagta ttagcgtta tcagtgaaa gtggctgtt cgtacagga cagacgagg atgaagtag caacccctt ttgaagaac cccaattaa gcgaggaag ctccagttg gtgagcctg aaattgtgg aagaaccac cgccgctag acgaggtca aaagcatcg aaaaattcc tctatctcg ccacgagcg ccttttgcg ctgttataa taatcgggt tctcggttg cggtgcaac aataccttt acaattctt ctgatgtag ataattatg aaccggatt tcgcgttcc ctcgtgtac aaaatatca aaacagacc ctccaaaaa agtaccccc cgaagcgga aaagtcaca tcaaactag acgaattag aaaataagt ttgctaaat tagaagtag acaacgaaa ggcatgaca agatcgcac cggctggtg gagacaatg acccccgag ccgagggaa atctcaaat ctaattata aacttaaca aaaataaaa gcccagtca aatgtaaca aagaaaatt gcgacgcag tacgaaacg accaagaaa atcgaaatg acgagacct acgacaaaa aaatcaagg aactgaaag tgaaggagg attatctca gagagaagg aaaagtttc tcattgaga aagaacacg acttgttga agaaggaga tcaagctta acagggaga aggagagct acgacaata ccaaaaatg acaagaaaa gacattata aggaggatg atgctaaaa agcccaaag accgcaaga agaaacaaa aactggccg aggagccgt atagaaaac gcaaccttt cgggccagt ggtatataa gcatgcacg aagctaggg acaacatgc ggcagaaaa aacggtcgc tgtttaaaa aaaacaagg ctcattggt acttccagt ggatggtgg atagagcaa ggcttcgcg tcaagtgaa ctatttttc taaattgtc actctgcat tttatttga catttcgtt tgagtatta ttgttttta tgtactttt tatttcgta attgtgaat tttttcgat tattttaat aaagactca cttatttgg ggttgtttt aatcgagtg ggagctggg cgggtaagg cgaataaaa atcacttgt atgaaataa accctctga tttattctt aaaattaag gtcatacaa tcgcttctt gttaagcat actacttaa taatagtaa taataatag taataacca ctactgacg tttacacgt ccaacaatt gaatcaaaa atatcaatg gcactggtt aaatatcac ccgattcta taagtatat gcgtaagta acaaatact tttgttatt tatttacgg tataattgt aacattgga gtacatgag taaattttc caaattatt agatcttca cgtgtttga ctaacagct tcctaacag tattattag tgattaaaa gcttgaatt agttaaaaa atatgagta cagacctgg ttaagtatt aatgtttaa agcttgaaa acaattgta ctacaatta cgtattaag taaagtaaa tgtaaatat tagacggta taatctcgt ttccgtttt aaatttagt ataaaaaat ctacacctc taacttcat acaccgagt tagttattg acttggatg ttacgatgg atggatacg cgatggatt cacctagtg tttacatta tatcacatt gttatattt cttaaccaa taagctttt gaatttcgt cgatgaagg tctaaaaat ttgtactaa ggaaaaggg aagtaaacg aaatgcaga gtgcccaga agagcaata cttgttaat cagagcgga gggataggt acatcagtc ggggtcact ctgctatac gcgactaga aatgagaag ataaaggag tcgggatga gcatgacgt gggggacgc ccgggactg tccgtcgca ccctcgctc ggccgcggc tgctttaaa ggtactcaa tcacagtag atttaacaa ccgacacgg ccacaccgc cggagcccc caaaaaacc ctctcatct accaagcac caagcacca aacccgggg gctccgacg ctgcgtggt gccgcaata tacaatagt atataaata tatgttata tacatgtga actataatg ccatttggt gcagctggc aactcacgt atagtcgta atatacagt tgagctggc gttgcgctg tgagcaagc tcggttata acgtctcta atataacta agctacggt aacagtaat attattgta atactaagg cacagaccc agctcatta cttgttcgc tcaacaagc acgtttgtg gaagacaag catcgctgg atattctac ccaactctg gggacgccc acccccgca ccgccatct cccaccaaa caatcggca taaaccaaa gtagcgttg gattcgtcc cgggcaacg caactatca ttagaaatc cacagaaat ccctgtaat ccgcataaa tcccaagta ctactctca agtcgctca ctctttttc gacactgtc ctgttttat atccataca tagaacatc taacgaacc aaactgaat ggtacaatt ttcggataa aaccaagaa acaagactt taccgagca gactactaa cacctattt tctatttat aatttccga tgaaagaat tcgttttcg ctatgaaaa ttgccagtg aaaaaattt tcaacaatt tttctggtt cgttataag cagacattg ctgcacaag taatatctc tatcttgta aatttggcg ctcaaattg aaaagtttt ttcatcgat ttttataaa atgcgaaga acatacaaa tcatacaaa aaaactttg aagtagagt ccgacgtat ctaagaaaa tcgcgattt ccaattgtt tcattattt ggacctaag tatttgatt taaagtcat agtctgacc tattgaaaa taaaatata acccaagtt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
