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Gene information
General information
TC004799 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7398186..7399977
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004799
    iBeetle screen iB_06623

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber sometimesstage sequence irregular - Number of animals showing the phenotype: 2

    vitellogenic egg chamber posteriorfused - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004799 RA
      MGKDYYKIL GLSKGASDD DIKKAYRKL ALKYHPDKN 
      KAPGAEERF KEVAEAYEV LSDKKKRDI YDSYGEEGL 
      KGGVPGNSG FGNSSFSYT YHGDPRATF AQFFGNASP 
      FAAFFSGNH SMFGFPDDD MDVDDPFAS LNVGPTRAG 
      PGGAFRSHS FNFHGTPNR SKDKVQDPP IEHDLYVSL 
      EDITKGCTK KMKISRRVL QADGTAKKE DKVLTINVK 
      PGWKAGTKI TFQREGDQG RNKIPADIV FIIRDKPHP 
      LFKREGSDI RYTAKISLK QALCGCTIE VPTMSSTKI 
      PLHYTSEII KPNTVRRIQ GYGLPLPKE PSRRGDLIV 
      NFDIRFPEA LTQSAKDIL YDTLPN
    CDS sequence
    • >TC004799 RA
      atgggcaaa gattactac aaaatcctc ggacttagc 
      aaaggtgcc agtgacgac gacatcaaa aaggcctac 
      cgaaagctg gccctgaag taccacccc gacaagaac 
      aaggccccg ggggcggag gagcgcttc aaggaggtg 
      gccgaggcg tacgaggtg ctctcggac aagaagaag 
      cgcgacatc tacgacagc tacggcgag gagggcctg 
      aagggcgga gttccgggc aactccggc ttcggcaac 
      tccagcttc agctacacc taccatggg gacccgcgc 
      gccaccttc gcgcagttc ttcgggaac gcgagtccc 
      ttcgcggcg ttcttcagc ggaaatcac agcatgttc 
      gggttcccg gacgacgac atggacgtg gacgacccc 
      ttcgcgtcg ctcaacgtg ggcccgacg cgggcgggg 
      cctggcggc gccttccgg agccactcg ttcaatttc 
      cacggcacc cccaatcgg agcaaggat aaggtgcag 
      gacccgccc atcgagcac gacctctac gtctcgttg 
      gaggacatc acgaagggc tgcaccaag aagatgaag 
      atcagtcgg agggtgctg caggcggac gggacggcc 
      aagaaggag gacaaggtc ttgacgata aatgtgaag 
      ccggggtgg aaggcgggg acgaaaatt acattccag 
      cgggagggg gatcagggg aggaataag atcccggct 
      gatattgtt tttattatc agggacaag ccgcatcct 
      ttgttcaag cgcgaaggg agcgatatc agatacacg 
      gcgaaaatc tcactcaaa caggctttg tgtggttgc 
      actattgag gttcctacg atgtcttcc acgaagata 
      cctctgcat tacacctcg gagattatt aaaccaaat 
      acggtgagg cgcatccag ggctatggg ctgccccta 
      ccgaaggaa ccctcccgc aggggcgac ttgatagtt 
      aacttcgat atcaggttt cccgaggct ctcacgcag 
      tcggctaaa gatatacta tacgatact cttccgaac 
      taa
    mRNA sequence
    • >TC004799 RA
      cgggtaaaa aggcactgt cagtgcgag tgtttgttt 
      taattgggg gcgccaaag tgtgtggat tattattat 
      agctaagtt tgttgaata atgtggtgg ataagggta 
      caaaaatag gtcgaggag tcgcgaatt tagaaattt 
      ataaccttt aaatataag cagctcgag cacgaaatc 
      gcagttcat ttggctgtc ggtcgttgt gcgtgtcac 
      tcgtccgtt cgtttgaca ataatcggg tggtacgtg 
      cgtgtttcc caaccctca gtgcttgaa aatgggcaa 
      agattacta caaaatcct cggacttag caaaggtgc 
      cagtgacga cgacatcaa aaaggccta ccgaaagct 
      ggccctgaa gtaccaccc cgacaagaa caaggcccc 
      gggggcgga ggagcgctt caaggaggt ggccgaggc 
      gtacgaggt gctctcgga caagaagaa gcgcgacat 
      ctacgacag ctacggcga ggagggcct gaagggcgg 
      agttccggg caactccgg cttcggcaa ctccagctt 
      cagctacac ctaccatgg ggacccgcg cgccacctt 
      cgcgcagtt cttcgggaa cgcgagtcc cttcgcggc 
      gttcttcag cggaaatca cagcatgtt cgggttccc 
      ggacgacga catggacgt ggacgaccc cttcgcgtc 
      gctcaacgt gggcccgac gcgggcggg gcctggcgg 
      cgccttccg gagccactc gttcaattt ccacggcac 
      ccccaatcg gagcaagga taaggtgca ggacccgcc 
      catcgagca cgacctcta cgtctcgtt ggaggacat 
      cacgaaggg ctgcaccaa gaagatgaa gatcagtcg 
      gagggtgct gcaggcgga cgggacggc caagaagga 
      ggacaaggt cttgacgat aaatgtgaa gccggggtg 
      gaaggcggg gacgaaaat tacattcca gcgggaggg 
      ggatcaggg gaggaataa gatcccggc tgatattgt 
      ttttattat cagggacaa gccgcatcc tttgttcaa 
      gcgcgaagg gagcgatat cagatacac ggcgaaaat 
      ctcactcaa acaggcttt gtgtggttg cactattga 
      ggttcctac gatgtcttc cacgaagat acctctgca 
      ttacacctc ggagattat taaaccaaa tacggtgag 
      gcgcatcca gggctatgg gctgcccct accgaagga 
      accctcccg caggggcga cttgatagt taacttcga 
      tatcaggtt tcccgaggc tctcacgca gtcggctaa 
      agatatact atacgatac tcttccgaa ctaagtgaa 
      ttctgtgaa aggagcata agtgtattg tacgtttta 
      gtacaagcg agtgaatga ggtattttt gtaattgaa 
      tgtaaataa tgcttatta aaatgtatt ataacgcaa 
      ccgcgggtc ggatatgta tttttaagt ctgacttgc 
      accgctcaa gatctaatt gtacataat cgtcgaaaa 
      agtattgta tatataatg aaagccgat ggaagtgca 
      tgaactcgc ctgcagaaa ttatttatt tttttaaag 
      attttaatt tgttgttat tgttgttcc tagtaacga 
      cgcgctaaa atgatgact aattaattt tgtaacttg 
      agagtaata aagaagttt atttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY