Status
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Gene information
General information
TC011302 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19299303..19303224
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011302
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06622

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    follicular epithelium partially potentially decreased - Number of animals showing the phenotype: 3

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011302 RA
      MEHLRDSGK VDPDYESYN SALELNEEE DKKENLIKI 
      RNIIEEEYN KEIFERQEQ IEQIELQIC KVRKILHLL 
      RYALIMSYY KKKELEYNG TEDEASTSD PLLAPDKQN 
      RIHPALKKL LGKNVSSLD HLNLKDKRK TTARSPRIP 
      DPPTPPESK KIKLDPDII PQTPPKVQP PEAVTRNRK 
      KTKHRVVIG NISKWMPSS EDDLLTHKW MVYVRGPKD 
      TPDVSHFVD KVVFYLHPS YKPHDVVEV SESPFHLAR 
      RGWGEFPVR VQIFFKVIL NKPIDVVHN IKLDKTFSG 
      RQTLGNETI VDVFLYDSE VKAEVEHDY CSDVETKIE 
      EVNFDHCYC MPASSEGLP KLKKEKIEQ DEDNKLIYG 
      LESYCGENH EKITPNQLV LNRKDIRFN NTKILVFGQ 
      KLEGCVLTK LIQNHSRNA KSEANNFKL VLPSHRFKN 
      MGEALPFLF KRLPLFSDL ANNANYKRI YPFTASSLK 
      EFVSWNVGK RRSSEWSRA RLIKKILTS EQIFGSENW 
      NTKTILMYG RSHDYTPFI SFGLFNRES EEKQLLQSC 
      YSSSPTIGC RNHAIEQQE ITVNIESDS ESPKKNHSV 
      IDITDPHLK LECSFIRQT ALDCGIILK PEEIVEGVK 
      FNGAEMMIL EAVKCLAEN LIRRGKNHR VCTKISSNS 
      STSVINRED VEMAINERP EMFSLKYFR NERKNFEYF 
      S
    CDS sequence
    • >TC011302 RA
      atggaacat ttgagggat tctggaaaa gttgatcct 
      gattatgag agttataat tcagctttg gaactaaat 
      gaggaagaa gacaagaag gagaattta attaagata 
      agaaatata atcgaggag gaatacaat aaagaaatt 
      ttcgagaga caggaacag attgaacaa atcgaatta 
      caaatatgc aaagtccgc aaaatttta catttgttg 
      cgctacgcc ttaatcatg tcctactac aagaaaaaa 
      gagttagag tacaacgga accgaggac gaagcctcc 
      acttccgat cccctcctt gcccctgac aagcagaac 
      cggatccac ccagccctc aaaaaactc ctcggtaaa 
      aacgtcagc agccttgac cacttgaac ctcaaagac 
      aagcgaaaa actactgcc agaagcccc cgcatcccc 
      gaccctcca acaccccca gaaagcaaa aaaattaag 
      ctcgacccc gatatcatc ccccaaacg ccccccaaa 
      gtccaaccc ccggaggcc gtcacgcgg aaccggaaa 
      aaaactaaa catcgtgta gtcattgga aacatttca 
      aaatggatg ccgtcatct gaagacgat cttctgacc 
      cacaagtgg atggtgtac gtaaggggc cctaaggac 
      acccccgac gtgtcgcat ttcgtcgat aaagtcgtt 
      ttctacctc cacccgtcg tacaaaccc cacgatgtg 
      gtggaagtc agcgaatcg ccgttccat ctagcacgc 
      cgtgggtgg ggcgagttt ccagtccga gtacaaatc 
      ttttttaaa gtgatttta aataaacca atcgatgtt 
      gtgcacaat attaagttg gataagacg ttcagtggg 
      cgacagacg ttgggaaat gagacgatc gttgatgtt 
      tttctgtac gacagtgaa gttaaagcg gaagttgaa 
      catgattat tgttccgat gttgagaca aaaattgaa 
      gaagttaat tttgaccac tgttactgt atgcctgcc 
      tcgagcgag ggcttgccc aagctgaag aaagaaaag 
      attgaacaa gacgaagac aataaatta atctacggg 
      ttggagtcg tattgcggg gaaaatcac gagaaaata 
      acaccaaat caactagtt ttaaatcgg aaggatatc 
      aggtttaat aatacgaaa attctggtt ttcgggcaa 
      aaactagaa ggctgcgtt ttgaccaaa ctgatccaa 
      aatcattcg agaaatgct aaaagtgag gcgaacaat 
      tttaaacta gttttgccc tcacacagg ttcaaaaat 
      atgggggag gcgcttcct ttcctgttc aaaaggttg 
      ccccttttt agcgattta gcgaataat gcgaattac 
      aaacgcatt tacccgttt actgcctcg agtttgaaa 
      gagtttgta agctggaat gttgggaaa aggcggagt 
      tctgagtgg agtcgcgca agacttatt aaaaaaatt 
      ctaacttcg gaacaaatt ttcggttcc gaaaattgg 
      aacactaag acgattttg atgtatgga aggtcacac 
      gactacaca ccatttatt tctttcggc cttttcaat 
      cgagagtca gaggaaaaa caattgctt cagtcgtgt 
      tactcttca agcccgacc attggttgc cgcaaccac 
      gcaattgaa cagcaagaa atcacagtc aacattgag 
      tcagattcg gagtctccg aaaaaaaat cattctgtg 
      attgacatc accgaccct catttgaaa ctcgagtgt 
      tcgttcatc cgccaaacg gccctcgac tgtggcatt 
      atcctcaag ccggaggaa atcgtagaa ggcgtgaag 
      tttaacggt gctgaaatg atgattctc gaagctgtt 
      aaatgtctc gccgagaat ttgatccga cgagggaag 
      aatcataga gtttgcacg aaaatttcg agtaattct 
      agtacaagt gtaataaac cgggaggac gtcgagatg 
      gcaatcaat gagcggccg gagatgttc tccttgaaa 
      tatttcagg aacgagcgg aaaaatttt gagtatttc 
      tcgtaa
    mRNA sequence
    • >TC011302 RA
      tcggtgtaa ggaggagac caaaataaa acccaaaaa 
      tctgataac tggaaaaaa cgtaaacga aaatggagc 
      tcgtgattt aagtgcgaa aagtgtgac ttttgcggg 
      tttgttgtg gcgaaatgg caagacatt tgaggtgat 
      taattgggg aaggttgag taaaatgga acatttgag 
      ggattctgg aaaagttga tcctgatta tgagagtta 
      taattcagc tttggaact aaatgagga agaagacaa 
      gaaggagaa tttaattaa gataagaaa tataatcga 
      ggaggaata caataaaga aattttcga gagacagga 
      acagattga acaaatcga attacaaat atgcaaagt 
      ccgcaaaat tttacattt gttgcgcta cgccttaat 
      catgtccta ctacaagaa aaaagagtt agagtacaa 
      cggaaccga ggacgaagc ctccacttc cgatcccct 
      ccttgcccc tgacaagca gaaccggat ccacccagc 
      cctcaaaaa actcctcgg taaaaacgt cagcagcct 
      tgaccactt gaacctcaa agacaagcg aaaaactac 
      tgccagaag cccccgcat ccccgaccc tccaacacc 
      cccagaaag caaaaaaat taagctcga ccccgatat 
      catccccca aacgccccc caaagtcca acccccgga 
      ggccgtcac gcggaaccg gaaaaaaac taaacatcg 
      tgtagtcat tggaaacat ttcaaaatg gatgccgtc 
      atctgaaga cgatcttct gacccacaa gtggatggt 
      gtacgtaag gggccctaa ggacacccc cgacgtgtc 
      gcatttcgt cgataaagt cgttttcta cctccaccc 
      gtcgtacaa accccacga tgtggtgga agtcagcga 
      atcgccgtt ccatctagc acgccgtgg gtggggcga 
      gtttccagt ccgagtaca aatcttttt taaagtgat 
      tttaaataa accaatcga tgttgtgca caatattaa 
      gttggataa gacgttcag tgggcgaca gacgttggg 
      aaatgagac gatcgttga tgtttttct gtacgacag 
      tgaagttaa agcggaagt tgaacatga ttattgttc 
      cgatgttga gacaaaaat tgaagaagt taattttga 
      ccactgtta ctgtatgcc tgcctcgag cgagggctt 
      gcccaagct gaagaaaga aaagattga acaagacga 
      agacaataa attaatcta cgggttgga gtcgtattg 
      cggggaaaa tcacgagaa aataacacc aaatcaact 
      agttttaaa tcggaagga tatcaggtt taataatac 
      gaaaattct ggttttcgg gcaaaaact agaaggctg 
      cgttttgac caaactgat ccaaaatca ttcgagaaa 
      tgctaaaag tgaggcgaa caattttaa actagtttt 
      gccctcaca caggttcaa aaatatggg ggaggcgct 
      tcctttcct gttcaaaag gttgcccct ttttagcga 
      tttagcgaa taatgcgaa ttacaaacg catttaccc 
      gtttactgc ctcgagttt gaaagagtt tgtaagctg 
      gaatgttgg gaaaaggcg gagttctga gtggagtcg 
      cgcaagact tattaaaaa aattctaac ttcggaaca 
      aattttcgg ttccgaaaa ttggaacac taagacgat 
      tttgatgta tggaaggtc acacgacta cacaccatt 
      tatttcttt cggcctttt caatcgaga gtcagagga 
      aaaacaatt gcttcagtc gtgttactc ttcaagccc 
      gaccattgg ttgccgcaa ccacgcaat tgaacagca 
      agaaatcac agtcaacat tgagtcaga ttcggagtc 
      tccgaaaaa aaatcattc tgtgattga catcaccga 
      ccctcattt gaaactcga gtgttcgtt catccgcca 
      aacggccct cgactgtgg cattatcct caagccgga 
      ggaaatcgt agaaggcgt gaagtttaa cggtgctga 
      aatgatgat tctcgaagc tgttaaatg tctcgccga 
      gaatttgat ccgacgagg gaagaatca tagagtttg 
      cacgaaaat ttcgagtaa ttctagtac aagtgtaat 
      aaaccggga ggacgtcga gatggcaat caatgagcg 
      gccggagat gttctcctt gaaatattt caggaacga 
      gcggaaaaa ttttgagta tttctcgta aaatgtccg 
      taaattgtg attaaatac actattttt ccaacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY