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Gene information
General information
TC031557
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004601
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6785365..6790153
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031557
    iBeetle screen iB_06619

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031557 RA
      MERRIKLKT LNSHITCKI CRGYLIDAT 
      TVTECLHTF CKSCLVKHL EENNTCPTC 
      NIVIHQSHP LQYISFDRT MQDIVYKLV 
      PELQKNEIT REREFYHQR GLPCPKDIP 
      PNPGELEEE KPSADVHAE SDYHRQDEQ 
      VNVCLECIS SNLKTLKRR FIRCSAQAT 
      ILHLKKFVA KKVLNGMEK YRDIDILCN 
      DELLGKDHT LKFVYVTRW RFQNPPLTL 
      QYRPRIDI
    CDS sequence
    • >TC031557 RA
      atggaaaga agaatcaag ttgaaaaca ttaaacagt 
      catataact tgtaagata tgtagaggg tatctaatt 
      gacgccacg actgtaaca gaatgtctt cataccttt 
      tgcaaaagt tgcctcgta aagcattta gaagaaaac 
      aacacctgt ccaacatgc aatattgta atccaccaa 
      tcccacccc ctacagtac ataagtttc gataggact 
      atgcaagat atcgtgtac aaattggtc ccagaactg 
      cagaaaaat gaaatcacc cgcgagaga gaattctac 
      caccaaaga ggtttacca tgtcctaaa gacattcct 
      ccgaacccg ggcgaactg gaggaggag aaaccatca 
      gccgacgtg cacgcagag tccgattac cacagacag 
      gacgaacag gtcaatgtc tgtctagag tgtattagt 
      tcaaattta aaaacgtta aagaggcga tttattcgc 
      tgttcggcg caagccacc atcttacac ctcaaaaaa 
      tttgtcgcc aaaaaggtc ctcaatgga atggaaaag 
      tacagagat atcgacatt ctatgcaac gacgagttg 
      ttaggcaag gaccacact ttaaaattc gtttatgtg 
      accaggtgg agatttcaa aacccgccc ctgactctg 
      cagtaccga cccagaatt gacatttaa
    mRNA sequence
    • >TC031557 RA
      gcactccac tccatcaac aatttttgt ttgtgtgtg 
      tcaattagg gttagaaca aattcagta caataatgt 
      ttttttatg aattatgat aatggaaag aagaatcaa 
      gttgaaaac attaaacag tcatataac ttgtaagat 
      atgtagagg gtatctaat tgacgccac gactgtaac 
      agaatgtct tcatacctt ttgcaaaag ttgcctcgt 
      aaagcattt agaagaaaa caacacctg tccaacatg 
      caatattgt aatccacca atcccaccc cctacagta 
      cataagttt cgataggac tatgcaaga tatcgtgta 
      caaattggt cccagaact gcagaaaaa tgaaatcac 
      ccgcgagag agaattcta ccaccaaag aggtttacc 
      atgtcctaa agacattcc tccgaaccc gggcgaact 
      ggaggagga gaaaccatc agccgacgt gcacgcaga 
      gtccgatta ccacagaca ggacgaaca ggtcaatgt 
      ctgtctaga gtgtattag ttcaaattt aaaaacgtt 
      aaagaggcg atttattcg ctgttcggc gcaagccac 
      catcttaca cctcaaaaa atttgtcgc caaaaaggt 
      cctcaatgg aatggaaaa gtacagaga tatcgacat 
      tctatgcaa cgacgagtt gttaggcaa ggaccacac 
      tttaaaatt cgtttatgt gaccaggtg gagatttca 
      aaacccgcc cctgactct gcagtaccg acccagaat 
      tgacattta attaatttt gtgtccctc tctgttgca 
      atattaaat gagtaggtt gtgattacg tttccacag 
      caacgccaa ttcatatag ttttataaa atagtacaa 
      atttttata aacgtttgt agataatca tttccaaca 
      ctagcattt tccagcatt tatttacaa ggcacttgt 
      tttttaatt cgttttaag ctatttttt aaataagtt 
      tgaggcgtt tgatactat ttccgagat ttctatttt 
      attactgtg atttctaac cagaggttt aatgccata 
      gggtaggta cttaaagta tcagacgct ttagataca 
      aagtttcaa agaaattca atcaattct agaaattgt 
      gctgtaaaa taatcttga cttgtactt cctattccg 
      ttgcctgaa gttttatta attttgtaa atagtgata 
      gggtcatgg aacttcatt ttatccccc acaacgtta 
      ttttaagca cgattctaa gctttaact aattgaata 
      tagcattag gacacttat tttaatggc acccaaact 
      tgtgttaaa ttgttttga atgtatagt gctgtattt 
      tgtgcaaga tgtgtagtg taactgttt tcgtattat 
      caaaaattg accagccgt tctatttac aaaatcaaa 
      aacctaaat ttttataca tttttaatg ttaggtaag 
      tgcggattc ttttgcata gcgaccttc tatcttttt 
      ttattggtg attattgtt aaaactcga ccaagccgg 
      tccagttct aaccatttt ccatactta attacataa 
      atttgtatg taaaaatta aatattttt taacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY