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Gene information
General information
TC004769 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6683850..6698697
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004769
    iBeetle screen iB_06617

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004769 RA
      MALNQDEGK QVLRVDQDS ETDLQALFD SVLKPDSKR 
      PLQVPWSMR KLPDSFFNP PSTGSKSIN HSRENSVDS 
      AFSSAASAS GSSTAAAAA PAPLPSAHH RAHSSPASL 
      QQTYAVGQA QATHHHIKQ RSYDVASKV EDNTPLPTG 
      WEQARTPEG QLYYLNHIT RTTTWEDPR KSLAAQAAA 
      QQHQSAEQL LSSHQVSQA QAPNSTPPA KVSTDVDLG 
      PLPEGWEQA QTPEGEIYF INHQTRTTS WFDPRIPTH 
      LQQRSPGTA SVSGQSWPS GSLSQSSPA KQQQFRLQL 
      LQFERDRLK LRQQEIRMQ QELMMRGSS NDLALDPFL 
      NDHSRQESA DSGLGLGTT YSMPHTPED FFDCTEDLV 
      PSLQLGEEF PTDLGEEFP TVILDDDVQ SLINPPTTK 
      PDNVLIWL
    CDS sequence
    • >TC004769 RA
      atggcattg aaccaggac gagggcaag caggtcctg 
      cgcgtggac caggactcg gagacggac ctgcaggcc 
      ctcttcgac agcgtgctg aagccggac tcgaagcgg 
      cccctgcag gtgccctgg agcatgcgg aagctgccg 
      gactcgttc ttcaacccg ccgtccacg ggctccaag 
      tccatcaac cactcgcgc gagaactca gtcgactcc 
      gccttcagc tcggcggcc agcgccagc ggcagctcc 
      accgccgcc gccgccgcc cccgcgccg ctccccagt 
      gcccaccac cgggcccac agctcgccc gcctccctc 
      cagcagacc tacgccgtg gggcaggcg caggccacg 
      caccaccac atcaagcag cgctcctac gacgtcgcc 
      tccaaggtc gaagacaac accccgctg cccactggc 
      tgggagcag gcgcgcacc cccgaagga cagctctac 
      tatcttaac cacataact cggaccaca acttgggaa 
      gacccgcga aagtcgctg gctgcgcaa gcggcagcc 
      caacaacac caaagtgcc gaacagctt ttaagttct 
      catcaggtg tcgcaggcc caagccccc aattccacg 
      ccccctgcc aaagtgagt acagacgta gatttaggc 
      cctttgcct gaaggttgg gagcaggca caaactcct 
      gagggagaa atttatttc ataaatcat caaacacgt 
      actacttcg tggtttgac cctcgaatt cccacacat 
      ttgcaacag agatctccc ggcacggct tcggttagt 
      gggcagtcg tggccaagt ggctctttg tcgcaatcc 
      tcgccggct aaacagcaa cagtttcga ctacaactg 
      ttgcaattc gaacgtgac agactgaaa ttgcgacag 
      caagaaatt cgaatgcaa caggaactt atgatgcgg 
      gggagttcc aacgactta gcgctagac ccctttctg 
      aacgaccac tccaggcag gagtccgcc gacagcggc 
      ttaggtttg gggacgacg tattcgatg cctcacacg 
      ccggaggat ttttttgat tgtacggag gatttagtg 
      ccttcgctc caactgggc gaagagttc ccgactgat 
      ttgggcgag gagttcccg actgtcatt ttagacgat 
      gatgtacaa tcgttaata aatccgccg actacgaag 
      cccgacaat gtgctcatt tggttgtaa
    mRNA sequence
    • >TC004769 RA
      acttttaaa ggtggcgct agtgactta agtaagcag 
      ctaagtcgt ggaggcgta ttaatggaa tcagtaatc 
      gtcgtaaag tgcgtgtat tgggatgcc ctcggtgga 
      tatcgtgta aaacaatgc agtaagaat gcgcgcggt 
      taagtggaa gacaggtgt atgaggtga gcattccga 
      acaattccg aagtaggaa aaccagacc cagaagcag 
      caaggttgt cgtccgcca cgaagttgg gcccgaagt 
      cacgccaag tccggttta ggggatgag aacagggac 
      tagagtgcc gtagtggtg tggtgaaga aatcaatcg 
      gggacgtag aataaagcg atatcggtg tcggtgtat 
      cttggtgca gggacggca gggaagggg gcgcgcact 
      cgccgtgtg aacctattg gttgatgtc tgaaacttt 
      gttgactgg ctggtagtc gagtgtcgg agtcgatga 
      atcgttggt tgtcctggt agtgtccgc gtccgcgtc 
      ggtgtcggt gtaagttgg tgtggttgt ggcagtgag 
      tcctcgccg aagatggca ttgaaccag gacgagggc 
      aagcaggtc ctgcgcgtg gaccaggac tcggagacg 
      gacctgcag gccctcttc gacagcgtg ctgaagccg 
      gactcgaag cggcccctg caggtgccc tggagcatg 
      cggaagctg ccggactcg ttcttcaac ccgccgtcc 
      acgggctcc aagtccatc aaccactcg cgcgagaac 
      tcagtcgac tccgccttc agctcggcg gccagcgcc 
      agcggcagc tccaccgcc gccgccgcc gcccccgcg 
      ccgctcccc agtgcccac caccgggcc cacagctcg 
      cccgcctcc ctccagcag acctacgcc gtggggcag 
      gcgcaggcc acgcaccac cacatcaag cagcgctcc 
      tacgacgtc gcctccaag gtcgaagac aacaccccg 
      ctgcccact ggctgggag caggcgcgc acccccgaa 
      ggacagctc tactatctt aaccacata actcggacc 
      acaacttgg gaagacccg cgaaagtcg ctggctgcg 
      caagcggca gcccaacaa caccaaagt gccgaacag 
      cttttaagt tctcatcag gtgtcgcag gcccaagcc 
      cccaattcc acgccccct gccaaagtg agtacagac 
      gtagattta ggccctttg cctgaaggt tgggagcag 
      gcacaaact cctgaggga gaaatttat ttcataaat 
      catcaaaca cgtactact tcgtggttt gaccctcga 
      attcccaca catttgcaa cagagatct cccggcacg 
      gcttcggtt agtgggcag tcgtggcca agtggctct 
      ttgtcgcaa tcctcgccg gctaaacag caacagttt 
      cgactacaa ctgttgcaa ttcgaacgt gacagactg 
      aaattgcga cagcaagaa attcgaatg caacaggaa 
      cttatgatg cgggggagt tccaacgac ttagcgcta 
      gaccccttt ctgaacgac cactccagg caggagtcc 
      gccgacagc ggcttaggt ttggggacg acgtattcg 
      atgcctcac acgccggag gattttttt gattgtacg 
      gaggattta gtgccttcg ctccaactg ggcgaagag 
      ttcccgact gatttgggc gaggagttc ccgactgtc 
      attttagac gatgatgta caatcgtta ataaatccg 
      ccgactacg aagcccgac aatgtgctc atttggttg 
      taataaagt gcctactta agtagctca gcttctacg 
      aatgtttta tattgatag aaatttaag acaaagttt 
      ggcagttgg atggtaaag actgtcgtc tgtatcgtt 
      attttgcaa tatgtacca tttgaatac gctttaaat 
      ggcaataat aatcaaaac atattttta atgttatta 
      taattttac atttatcgc atttgtaat tctcaaatc 
      agttactgt tgggtatgt ttttcatac atatgtatt 
      ttatagttt gtaattttt aagttagag ttgtagttt 
      atggtgcag atgatattt tgtcagttt tggtaagat 
      atcgatcaa attattgag tactgaacg caaaattca 
      atatttatt tattgttgt attagagtt aattattta 
      acaattttt atttatgtc atgtataac ttttaacct 
      ctgttccaa atctaacgt tgtgataat gttttcgca 
      aaattttat taattggta ggaatactt aactgtatc 
      cctatttaa ttttacttg ttactgtat gtgcaatgc 
      acccctgct cctcccagg accagactt gttgcatcg 
      cgaaggatt caactcgta taaatatgc tcacactga 
      actaatccc tccattttg tgcctttaa ttggtaacg 
      tatatgtat atattttat tgacaacgg ttacttgta 
      gtcttagtt ttacaattt ctaaaatat attttacac 
      taacatatt gtaacagtg cctttcaca tttactcta 
      gtgtttaag tgcttattg tatcaccac caattggta 
      cagctgtga ttaagaatc agaaatttt ggaccgttt 
      acaggtgtt attagctgc aaaattcac ttcacgaag 
      caatactca acgcaatca gtcttgtgg ctactaaca 
      ctgattttt catttgtgg gccaaccaa gcgaaatgt 
      tagccgatg agggagcga atcttaatt tcaatcaag 
      ctttcagta aggagaaaa gagatgccg cttgattgg 
      cctactgtt ttattgtga aattattgt attagttta 
      taattgtaa atatatatt ggaaatatt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY