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Gene information
General information
TC004611 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6640051..6648155
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004611
    iBeetle screen iB_06615

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004611 RA
      MSLYDPYQE LELYLERAK NEITQVFEN GTDEPAKNP 
      VPEEDIIKN ENKTDYELD PFLFQFNAT IKSDLSELL 
      SRYSEKQTG NEVNYTSVN NIVNSNTDY LNSNVENLS 
      ILNKDSDLC NRYGEEKKN VNQDSLLGS IAIGDYSHT 
      NVATQTSPS LSPTSTNLT WASECSSSP CLTCMSDND 
      LIDDDSHSA SSADGKGQL STWSYKPNW HQILSTKSS 
      SAPVLKKVP IKVSKQSRS QSDRHLAEI EAAEACKWL 
      RATGFPQYA QMYEDLQFP IDLSTVAQD HPLLEPDVL 
      HSLFRRLQI LNSCVHLHQ QRIAHNTDE SEDECCALS 
      DNWTYQTDI RRWSRACKN QPEPEKNSQ EKDDVFEQY 
      TESPRDKLR RAGSTKFRR RRRDGTIFS EGGSPQLDR 
      LDSLTHQLA DLKTCELNH VSDSECTPK RNQRTKSFD 
      NTDSWLTSQ ISSDDRVLW HALPQEEEQ SPQKTVNLE 
      NGGPSMFSL SCTQLQVLR KLALLKLTA HMEKHCPSH 
      RTGWNWDLP KFIRKMKTP AYKDRSIFG VPLTIMLQR 
      TGQSIPRNI EEALQWLHQ NAADHVGLF RKSGVKSRI 
      QILRNMVDA TTEILNYND QQSYDVADM IKQYFRELP 
      ETLLTNKLS ETFILIFQY VPPYLRRES VLCAILLMP 
      DEHVEVLQF LLHFLLQIA EHATTNQMN ESNLALCFA 
      PSLFHYSQS SFKQNFGSP HPKELAETR AGHDCLLYF 
      LKNYNALFK VPKEFVNQC KTSEFRESK AVKLSELGK 
      NIGGWREYL QECQMALLR EVKERNRGW IVVSGHNPR 
      VEVSYKKVA DGHPLRLWK VSAEVEAPP LEVLHRIIR 
      ERHLWDPEL HSAKIVSQI DKNCEVFQY VRRKIVPLP 
      NEEYCVVRT WRTDLPKDS CLVIETSVE HQDAVPIPN 
      TVRGIVLAS RYLIEPCGS GRSKLLHLS RVDSMGKTP 
      EWYQKNYGH ICALFVANI VSSFYHVAA GPESKV
    • >TC004611 RB
      MSLYDPYQE LELYLERAK NEITQVFEN GTDEPAKNP 
      VPEEDIIKN ENKTDYELD PFLFQFNAT IKSDLSELL 
      SRYSEKQTG NEVNYTSVN NIVNSNTDY LNSNVENLS 
      ILNKDSDLC NRYGEEKKN VNQDSLLGS IAIGDYSHT 
      NVATQTSPS LSPTSTNLT WASECSSSP CLTCMSDND 
      LIDDDSHSA SSADGKGQL STWSYKPNW HQILSTKSS 
      SAPVLKKVP IKVSKQSRS QSDRHLAGD LSVKVNDNW 
      PVRKAEQLW RQMRNHKIA EIEAAEACK WLRATGFPQ 
      YAQMYEDLQ FPIDLSTVA QDHPLLEPD VLHSLFRRL 
      QILNSCVHL HQQRIAHNT DESEDECCA LSDNWTYQT 
      DIRRWSRAC KNQPEPEKN SQEKDDVFE QYTESPRDK 
      LRRAGSTKF RRRRRDGTI FSEGGSPQL DRLDSLTHQ 
      LADLKTCEL NHVSDSECT PKRNQRTKS FDNTDSWLT 
      SQISSDDRV LWHALPQEE EQSPQKTVN LENGGPSMF 
      SLSCTQLQV LRKLALLKL TAHMEKHCP SHRTGWNWD 
      LPKFIRKMK TPAYKDRSI FGVPLTIML QRTGQSIPR 
      NIEEALQWL HQNAADHVG LFRKSGVKS RIQILRNMV 
      DATTEILNY NDQQSYDVA DMIKQYFRE LPETLLTNK 
      LSETFILIF QYVPPYLRR ESVLCAILL MPDEHVEVL 
      QFLLHFLLQ IAEHATTNQ MNESNLALC FAPSLFHYS 
      QSSFKQNFG SPHPKELAE TRAGHDCLL YFLKNYNAL 
      FKVPKEFVN QCKTSEFRE SKAVKLSEL GKNIGGWRE 
      YLQECQMAL LREVKERNR GWIVVSGHN PRVEVSYKK 
      VADGHPLRL WKVSAEVEA PPLEVLHRI IRERHLWDP 
      ELHSAKIVS QIDKNCEVF QYVRRKIVP LPNEEYCVV 
      RTWRTDLPK DSCLVIETS VEHQDAVPI PNTVRGIVL 
      ASRYLIEPC GSGRSKLLH LSRVDSMGK TPEWYQKNY 
      GHICALFVA NIVSSFYHV AAGPESKV
    CDS sequence
    • >TC004611 RA
      atgagctta tatgatccg tatcaagag ctagagctc 
      tatttggaa cgtgctaaa aacgaaata acccaagtt 
      tttgaaaat ggaacagat gagcctgca aaaaatcca 
      gttcccgag gaggatatt attaaaaac gagaataaa 
      acggactac gagctggac ccttttctt ttccaattt 
      aacgcgacc ataaagtca gatttgtcc gaattgttg 
      tcaaggtat tcggaaaag caaactgga aacgaggtc 
      aattatacc agtgtcaat aatatagtc aacagtaac 
      accgattac ctcaattct aatgttgaa aatttatct 
      attctaaat aaagacagt gatttatgt aaccgctat 
      ggcgaagag aaaaaaaat gttaatcaa gattcattg 
      ctaggttct attgctatt ggtgattac agtcataca 
      aatgtggca acgcaaaca tctccttcg ctctcgcca 
      acttcgacg aatttaaca tgggcgtcc gagtgttca 
      tcatctcct tgcttaact tgtatgtcc gacaacgat 
      ctgatcgat gacgactcc cattcagcg tcgtctgct 
      gatggcaag ggacaactg tccacttgg agctacaaa 
      ccgaattgg catcagatc ctaagtacg aagtcttct 
      tcagcacct gttttgaag aaggtgccc atcaaggtg 
      agcaaacaa tcccgatca cagtcggac cgacacctg 
      gctgaaatt gaagctgcg gaagcatgc aaatggttg 
      cgagcgacg gggtttccc cagtatgcc cagatgtac 
      gaagatctc cagtttccc atcgacctt tcaactgtt 
      gcgcaagat catcctctt ttagaacct gacgttttg 
      cattcgctc ttccggcga ttgcaaatt ctcaatagc 
      tgtgtgcat ttgcaccag cagaggatc gcccacaac 
      accgatgaa tcggaagac gaatgttgc gccctcagc 
      gacaactgg acgtaccag acggacata cgaaggtgg 
      tcgcgggcg tgcaagaac caacctgag ccggagaag 
      aactcgcaa gaaaaagac gatgttttt gagcaatat 
      accgagagt ccgcgagat aagttaagg agggccggt 
      tcgacaaaa ttcagacgg aggaggaga gatggcact 
      atcttcagc gaaggcggt tcaccacaa ctcgacaga 
      ctcgactca ctcacccac caattggct gacctaaaa 
      acatgcgaa ctgaatcac gtctcagat tccgaatgt 
      actccgaag cgcaatcaa aggaccaag tcattcgat 
      aacacagat tcatggttg acctcacag atttcatca 
      gatgacaga gttttatgg cacgctttg ccacaagaa 
      gaagaacaa tctcctcaa aaaacagtc aatttggaa 
      aacggtggt ccttctatg ttcagtctc tcatgtaca 
      caattacaa gtcctccgg aagttagcg cttttgaaa 
      cttacggca cacatggaa aagcattgt ccgtcgcat 
      agaacgggc tggaactgg gatttaccg aaatttatc 
      cgcaaaatg aaaactccc gcgtataaa gacaggtca 
      atttttggc gtacctctc actatcatg cttcaaaga 
      acaggtcag tcgattcct cgaaatatt gaagaggcg 
      cttcagtgg ctacatcag aacgcggcc gaccatgtg 
      ggactcttt cgtaaatcg ggcgtcaag tcacgtata 
      caaatatta agaaacatg gtcgatgct actaccgaa 
      atactaaat tacaatgac caacaatcg tacgatgtt 
      gccgatatg attaagcaa tactttcgg gaactaccg 
      gaaacgctt ctcactaac aaactatcc gaaacattt 
      attttaatt tttcaatac gttccgcca tatttgcgc 
      cgtgagtca gtactctgt gctatttta ttaatgcca 
      gacgaacat gtcgaagtc ctccagttt cttttacat 
      ttcttatta caaatcgcc gaacatgcc actacaaat 
      cagatgaat gaaagcaat ctcgctcta tgtttcgcc 
      ccttcgctc tttcattac tcccagagt tcttttaag 
      caaaacttt ggctcccct catccgaaa gaactcgcg 
      gagactaga gcaggccac gattgcttg ttgtatttc 
      ctcaagaat tacaatgca ctattcaag gtgccaaaa 
      gaattcgta aaccagtgc aaaacaagt gagttcagg 
      gaaagcaaa gcggtgaaa ttatccgaa ctggggaaa 
      aacataggc ggttggagg gagtatctt caggagtgt 
      cagatggcg cttttgcga gaagttaaa gagcgaaat 
      aggggttgg attgttgtt tcgggccat aatccgaga 
      gtcgaagta tcgtacaaa aaagtggcc gatggccac 
      ccgctccgc ctttggaaa gtttccgca gaggttgaa 
      gcgcccccc cttgaggtc ttacacagg attatacgc 
      gagaggcat ttgtgggac cctgagtta cactcggct 
      aagatagtg tcgcaaatt gacaaaaac tgcgaagtg 
      ttccaatac gtccgccgc aaaatcgtg ccgctgccg 
      aatgaggaa tattgcgtt gtgcggacg tggaggacc 
      gatctcccg aaagacagt tgcttggtt attgagact 
      tcggttgag catcaggat gctgtcccc attcccaat 
      actgtcagg ggcattgtg ttggcttcc cggtacctc 
      attgaacct tgcggctct ggacgctcg aaattattg 
      catttgtcc cgtgttgac tcaatggga aaaacaccg 
      gaatggtat caaaaaaat tacggacat atatgtgcc 
      ctctttgtt gctaatatt gtaagttcg ttttaccac 
      gtcgctgca ggaccagaa agtaaagta taa
    • >TC004611 RB
      atgagctta tatgatccg tatcaagag ctagagctc 
      tatttggaa cgtgctaaa aacgaaata acccaagtt 
      tttgaaaat ggaacagat gagcctgca aaaaatcca 
      gttcccgag gaggatatt attaaaaac gagaataaa 
      acggactac gagctggac ccttttctt ttccaattt 
      aacgcgacc ataaagtca gatttgtcc gaattgttg 
      tcaaggtat tcggaaaag caaactgga aacgaggtc 
      aattatacc agtgtcaat aatatagtc aacagtaac 
      accgattac ctcaattct aatgttgaa aatttatct 
      attctaaat aaagacagt gatttatgt aaccgctat 
      ggcgaagag aaaaaaaat gttaatcaa gattcattg 
      ctaggttct attgctatt ggtgattac agtcataca 
      aatgtggca acgcaaaca tctccttcg ctctcgcca 
      acttcgacg aatttaaca tgggcgtcc gagtgttca 
      tcatctcct tgcttaact tgtatgtcc gacaacgat 
      ctgatcgat gacgactcc cattcagcg tcgtctgct 
      gatggcaag ggacaactg tccacttgg agctacaaa 
      ccgaattgg catcagatc ctaagtacg aagtcttct 
      tcagcacct gttttgaag aaggtgccc atcaaggtg 
      agcaaacaa tcccgatca cagtcggac cgacacctg 
      gctggtgat ctcagtgtt aaagtaaat gacaactgg 
      ccggtgcgc aaggccgag cagctatgg aggcagatg 
      cgaaaccac aaaatcgca gaaattgaa gctgcggaa 
      gcatgcaaa tggttgcga gcgacgggg tttccccag 
      tatgcccag atgtacgaa gatctccag tttcccatc 
      gacctttca actgttgcg caagatcat cctctttta 
      gaacctgac gttttgcat tcgctcttc cggcgattg 
      caaattctc aatagctgt gtgcatttg caccagcag 
      aggatcgcc cacaacacc gatgaatcg gaagacgaa 
      tgttgcgcc ctcagcgac aactggacg taccagacg 
      gacatacga aggtggtcg cgggcgtgc aagaaccaa 
      cctgagccg gagaagaac tcgcaagaa aaagacgat 
      gtttttgag caatatacc gagagtccg cgagataag 
      ttaaggagg gccggttcg acaaaattc agacggagg 
      aggagagat ggcactatc ttcagcgaa ggcggttca 
      ccacaactc gacagactc gactcactc acccaccaa 
      ttggctgac ctaaaaaca tgcgaactg aatcacgtc 
      tcagattcc gaatgtact ccgaagcgc aatcaaagg 
      accaagtca ttcgataac acagattca tggttgacc 
      tcacagatt tcatcagat gacagagtt ttatggcac 
      gctttgcca caagaagaa gaacaatct cctcaaaaa 
      acagtcaat ttggaaaac ggtggtcct tctatgttc 
      agtctctca tgtacacaa ttacaagtc ctccggaag 
      ttagcgctt ttgaaactt acggcacac atggaaaag 
      cattgtccg tcgcataga acgggctgg aactgggat 
      ttaccgaaa tttatccgc aaaatgaaa actcccgcg 
      tataaagac aggtcaatt tttggcgta cctctcact 
      atcatgctt caaagaaca ggtcagtcg attcctcga 
      aatattgaa gaggcgctt cagtggcta catcagaac 
      gcggccgac catgtggga ctctttcgt aaatcgggc 
      gtcaagtca cgtatacaa atattaaga aacatggtc 
      gatgctact accgaaata ctaaattac aatgaccaa 
      caatcgtac gatgttgcc gatatgatt aagcaatac 
      tttcgggaa ctaccggaa acgcttctc actaacaaa 
      ctatccgaa acatttatt ttaattttt caatacgtt 
      ccgccatat ttgcgccgt gagtcagta ctctgtgct 
      attttatta atgccagac gaacatgtc gaagtcctc 
      cagtttctt ttacatttc ttattacaa atcgccgaa 
      catgccact acaaatcag atgaatgaa agcaatctc 
      gctctatgt ttcgcccct tcgctcttt cattactcc 
      cagagttct tttaagcaa aactttggc tcccctcat 
      ccgaaagaa ctcgcggag actagagca ggccacgat 
      tgcttgttg tatttcctc aagaattac aatgcacta 
      ttcaaggtg ccaaaagaa ttcgtaaac cagtgcaaa 
      acaagtgag ttcagggaa agcaaagcg gtgaaatta 
      tccgaactg gggaaaaac ataggcggt tggagggag 
      tatcttcag gagtgtcag atggcgctt ttgcgagaa 
      gttaaagag cgaaatagg ggttggatt gttgtttcg 
      ggccataat ccgagagtc gaagtatcg tacaaaaaa 
      gtggccgat ggccacccg ctccgcctt tggaaagtt 
      tccgcagag gttgaagcg ccccccctt gaggtctta 
      cacaggatt atacgcgag aggcatttg tgggaccct 
      gagttacac tcggctaag atagtgtcg caaattgac 
      aaaaactgc gaagtgttc caatacgtc cgccgcaaa 
      atcgtgccg ctgccgaat gaggaatat tgcgttgtg 
      cggacgtgg aggaccgat ctcccgaaa gacagttgc 
      ttggttatt gagacttcg gttgagcat caggatgct 
      gtccccatt cccaatact gtcaggggc attgtgttg 
      gcttcccgg tacctcatt gaaccttgc ggctctgga 
      cgctcgaaa ttattgcat ttgtcccgt gttgactca 
      atgggaaaa acaccggaa tggtatcaa aaaaattac 
      ggacatata tgtgccctc tttgttgct aatattgta 
      agttcgttt taccacgtc gctgcagga ccagaaagt 
      aaagtataa
    mRNA sequence
    • >TC004611 RA
      aataaattc attcattgt ggctgggag gttgatggg 
      aatactaaa ataactaaa taagttttc agtgttgaa 
      tgaatgtgc ccgctggtg ctggttaag tcgactttc 
      acttcatgt cttaagtga tatattatt atgaaattg 
      tcaaagatg tttcatgtg tgttgtaac atgaaagtc 
      aaagtgctt gagcgttgt ttttaggga acaggtggt 
      gtacatgct gcatctcct gtggcagtc gtggcccac 
      cactgttct tattctata tatttcact tcaaatatt 
      tccatcctg tttcccttc ccagcctcc cacttcaac 
      attcaactt tgcccactt tgccagttg ccacttttg 
      tattctgtg tttttattg ttagttgtg cggttgtct 
      gctggtggt acgtcactt gtttgttta gtcagttag 
      tcgaagata atacagagt ttggtcggt gacgaattc 
      ggttggtgc gtaacagca ctacgttta gcttgatgg 
      gaatgcggt ttgtggctg cactgactc agaatgctc 
      gtgttttta ccagtgcca agtaccaag tgccaaaat 
      ttacaaatc tgtgaaacc cacaaactc caagacact 
      tagtagaga ccatgtctc agaaggttc ttttactgt 
      ggtgaggaa agagggaga tgagcttat atgatccgt 
      atcaagagc tagagctct atttggaac gtgctaaaa 
      acgaaataa cccaagttt ttgaaaatg gaacagatg 
      agcctgcaa aaaatccag ttcccgagg aggatatta 
      ttaaaaacg agaataaaa cggactacg agctggacc 
      cttttcttt tccaattta acgcgacca taaagtcag 
      atttgtccg aattgttgt caaggtatt cggaaaagc 
      aaactggaa acgaggtca attatacca gtgtcaata 
      atatagtca acagtaaca ccgattacc tcaattcta 
      atgttgaaa atttatcta ttctaaata aagacagtg 
      atttatgta accgctatg gcgaagaga aaaaaaatg 
      ttaatcaag attcattgc taggttcta ttgctattg 
      gtgattaca gtcatacaa atgtggcaa cgcaaacat 
      ctccttcgc tctcgccaa cttcgacga atttaacat 
      gggcgtccg agtgttcat catctcctt gcttaactt 
      gtatgtccg acaacgatc tgatcgatg acgactccc 
      attcagcgt cgtctgctg atggcaagg gacaactgt 
      ccacttgga gctacaaac cgaattggc atcagatcc 
      taagtacga agtcttctt cagcacctg ttttgaaga 
      aggtgccca tcaaggtga gcaaacaat cccgatcac 
      agtcggacc gacacctgg ctgaaattg aagctgcgg 
      aagcatgca aatggttgc gagcgacgg ggtttcccc 
      agtatgccc agatgtacg aagatctcc agtttccca 
      tcgaccttt caactgttg cgcaagatc atcctcttt 
      tagaacctg acgttttgc attcgctct tccggcgat 
      tgcaaattc tcaatagct gtgtgcatt tgcaccagc 
      agaggatcg cccacaaca ccgatgaat cggaagacg 
      aatgttgcg ccctcagcg acaactgga cgtaccaga 
      cggacatac gaaggtggt cgcgggcgt gcaagaacc 
      aacctgagc cggagaaga actcgcaag aaaaagacg 
      atgtttttg agcaatata ccgagagtc cgcgagata 
      agttaagga gggccggtt cgacaaaat tcagacgga 
      ggaggagag atggcacta tcttcagcg aaggcggtt 
      caccacaac tcgacagac tcgactcac tcacccacc 
      aattggctg acctaaaaa catgcgaac tgaatcacg 
      tctcagatt ccgaatgta ctccgaagc gcaatcaaa 
      ggaccaagt cattcgata acacagatt catggttga 
      cctcacaga tttcatcag atgacagag ttttatggc 
      acgctttgc cacaagaag aagaacaat ctcctcaaa 
      aaacagtca atttggaaa acggtggtc cttctatgt 
      tcagtctct catgtacac aattacaag tcctccgga 
      agttagcgc ttttgaaac ttacggcac acatggaaa 
      agcattgtc cgtcgcata gaacgggct ggaactggg 
      atttaccga aatttatcc gcaaaatga aaactcccg 
      cgtataaag acaggtcaa tttttggcg tacctctca 
      ctatcatgc ttcaaagaa caggtcagt cgattcctc 
      gaaatattg aagaggcgc ttcagtggc tacatcaga 
      acgcggccg accatgtgg gactctttc gtaaatcgg 
      gcgtcaagt cacgtatac aaatattaa gaaacatgg 
      tcgatgcta ctaccgaaa tactaaatt acaatgacc 
      aacaatcgt acgatgttg ccgatatga ttaagcaat 
      actttcggg aactaccgg aaacgcttc tcactaaca 
      aactatccg aaacattta ttttaattt ttcaatacg 
      ttccgccat atttgcgcc gtgagtcag tactctgtg 
      ctattttat taatgccag acgaacatg tcgaagtcc 
      tccagtttc ttttacatt tcttattac aaatcgccg 
      aacatgcca ctacaaatc agatgaatg aaagcaatc 
      tcgctctat gtttcgccc cttcgctct ttcattact 
      cccagagtt cttttaagc aaaactttg gctcccctc 
      atccgaaag aactcgcgg agactagag caggccacg 
      attgcttgt tgtatttcc tcaagaatt acaatgcac 
      tattcaagg tgccaaaag aattcgtaa accagtgca 
      aaacaagtg agttcaggg aaagcaaag cggtgaaat 
      tatccgaac tggggaaaa acataggcg gttggaggg 
      agtatcttc aggagtgtc agatggcgc ttttgcgag 
      aagttaaag agcgaaata ggggttgga ttgttgttt 
      cgggccata atccgagag tcgaagtat cgtacaaaa 
      aagtggccg atggccacc cgctccgcc tttggaaag 
      tttccgcag aggttgaag cgccccccc ttgaggtct 
      tacacagga ttatacgcg agaggcatt tgtgggacc 
      ctgagttac actcggcta agatagtgt cgcaaattg 
      acaaaaact gcgaagtgt tccaatacg tccgccgca 
      aaatcgtgc cgctgccga atgaggaat attgcgttg 
      tgcggacgt ggaggaccg atctcccga aagacagtt 
      gcttggtta ttgagactt cggttgagc atcaggatg 
      ctgtcccca ttcccaata ctgtcaggg gcattgtgt 
      tggcttccc ggtacctca ttgaacctt gcggctctg 
      gacgctcga aattattgc atttgtccc gtgttgact 
      caatgggaa aaacaccgg aatggtatc aaaaaaatt 
      acggacata tatgtgccc tctttgttg ctaatattg 
      taagttcgt tttaccacg tcgctgcag gaccagaaa 
      gtaaagtat aaaaccaaa gatgtacat aataatgtt 
      gctaaatct aattatatt tttatatga agtaatcaa 
      tattattat atgaacatg ttgccgttt tacattatt 
      tgttatgga cagtaatta agtatctat gttatttta 
      ttttttact taatttttt attttatga aacattcac 
      attagttcg tttttctaa tcacgttac cgggaaagc 
      tatacacaa atacagtgg tgtcatatg gttagttag 
      gtacagttg tggtgcaag ggtttgacg tactatagc 
      tttcagtgt aatatattt aacaatttt atgtccatt 
      tgtgcaaga cctacactg ggttcaaat cgtagtcaa 
      aatgagact gaaaataac ttgaaaagt tcagtatac 
      ttaatcata aaaaattgc aataaatgc tcagtgtag 
      aacctcata ttttaatta agttaccat attcatgtt 
      tattaaata taaatatat tattgcata tttatcagc 
      tggatgaga acaggcttg tcagtgttt gtgctagtc 
      atataaatt gtttttaat cttactaga taatacttt 
      tccattaat ggaggccag tattgtatt ttttatgtt 
      g
    • >TC004611 RB
      aataaattc attcattgt ggctgggag gttgatggg 
      aatactaaa ataactaaa taagttttc agtgttgaa 
      tgaatgtgc ccgctggtg ctggttaag tcgactttc 
      acttcatgt cttaagtga tatattatt atgaaattg 
      tcaaagatg tttcatgtg tgttgtaac atgaaagtc 
      aaagtgctt gagcgttgt ttttaggga acaggtggt 
      gtacatgct gcatctcct gtggcagtc gtggcccac 
      cactgttct tattctata tatttcact tcaaatatt 
      tccatcctg tttcccttc ccagcctcc cacttcaac 
      attcaactt tgcccactt tgccagttg ccacttttg 
      tattctgtg tttttattg ttagttgtg cggttgtct 
      gctggtggt acgtcactt gtttgttta gtcagttag 
      tcgaagata atacagagt ttggtcggt gacgaattc 
      ggttggtgc gtaacagca ctacgttta gcttgatgg 
      gaatgcggt ttgtggctg cactgactc agaatgctc 
      gtgttttta ccagtgcca agtaccaag tgccaaaat 
      ttacaaatc tgtgaaacc cacaaactc caagacact 
      tagtagaga ccatgtctc agaaggttc ttttactgt 
      ggtgaggaa agagggaga tgagcttat atgatccgt 
      atcaagagc tagagctct atttggaac gtgctaaaa 
      acgaaataa cccaagttt ttgaaaatg gaacagatg 
      agcctgcaa aaaatccag ttcccgagg aggatatta 
      ttaaaaacg agaataaaa cggactacg agctggacc 
      cttttcttt tccaattta acgcgacca taaagtcag 
      atttgtccg aattgttgt caaggtatt cggaaaagc 
      aaactggaa acgaggtca attatacca gtgtcaata 
      atatagtca acagtaaca ccgattacc tcaattcta 
      atgttgaaa atttatcta ttctaaata aagacagtg 
      atttatgta accgctatg gcgaagaga aaaaaaatg 
      ttaatcaag attcattgc taggttcta ttgctattg 
      gtgattaca gtcatacaa atgtggcaa cgcaaacat 
      ctccttcgc tctcgccaa cttcgacga atttaacat 
      gggcgtccg agtgttcat catctcctt gcttaactt 
      gtatgtccg acaacgatc tgatcgatg acgactccc 
      attcagcgt cgtctgctg atggcaagg gacaactgt 
      ccacttgga gctacaaac cgaattggc atcagatcc 
      taagtacga agtcttctt cagcacctg ttttgaaga 
      aggtgccca tcaaggtga gcaaacaat cccgatcac 
      agtcggacc gacacctgg ctggtgatc tcagtgtta 
      aagtaaatg acaactggc cggtgcgca aggccgagc 
      agctatgga ggcagatgc gaaaccaca aaatcgcag 
      aaattgaag ctgcggaag catgcaaat ggttgcgag 
      cgacggggt ttccccagt atgcccaga tgtacgaag 
      atctccagt ttcccatcg acctttcaa ctgttgcgc 
      aagatcatc ctcttttag aacctgacg ttttgcatt 
      cgctcttcc ggcgattgc aaattctca atagctgtg 
      tgcatttgc accagcaga ggatcgccc acaacaccg 
      atgaatcgg aagacgaat gttgcgccc tcagcgaca 
      actggacgt accagacgg acatacgaa ggtggtcgc 
      gggcgtgca agaaccaac ctgagccgg agaagaact 
      cgcaagaaa aagacgatg tttttgagc aatataccg 
      agagtccgc gagataagt taaggaggg ccggttcga 
      caaaattca gacggagga ggagagatg gcactatct 
      tcagcgaag gcggttcac cacaactcg acagactcg 
      actcactca cccaccaat tggctgacc taaaaacat 
      gcgaactga atcacgtct cagattccg aatgtactc 
      cgaagcgca atcaaagga ccaagtcat tcgataaca 
      cagattcat ggttgacct cacagattt catcagatg 
      acagagttt tatggcacg ctttgccac aagaagaag 
      aacaatctc ctcaaaaaa cagtcaatt tggaaaacg 
      gtggtcctt ctatgttca gtctctcat gtacacaat 
      tacaagtcc tccggaagt tagcgcttt tgaaactta 
      cggcacaca tggaaaagc attgtccgt cgcatagaa 
      cgggctgga actgggatt taccgaaat ttatccgca 
      aaatgaaaa ctcccgcgt ataaagaca ggtcaattt 
      ttggcgtac ctctcacta tcatgcttc aaagaacag 
      gtcagtcga ttcctcgaa atattgaag aggcgcttc 
      agtggctac atcagaacg cggccgacc atgtgggac 
      tctttcgta aatcgggcg tcaagtcac gtatacaaa 
      tattaagaa acatggtcg atgctacta ccgaaatac 
      taaattaca atgaccaac aatcgtacg atgttgccg 
      atatgatta agcaatact ttcgggaac taccggaaa 
      cgcttctca ctaacaaac tatccgaaa catttattt 
      taatttttc aatacgttc cgccatatt tgcgccgtg 
      agtcagtac tctgtgcta ttttattaa tgccagacg 
      aacatgtcg aagtcctcc agtttcttt tacatttct 
      tattacaaa tcgccgaac atgccacta caaatcaga 
      tgaatgaaa gcaatctcg ctctatgtt tcgcccctt 
      cgctctttc attactccc agagttctt ttaagcaaa 
      actttggct cccctcatc cgaaagaac tcgcggaga 
      ctagagcag gccacgatt gcttgttgt atttcctca 
      agaattaca atgcactat tcaaggtgc caaaagaat 
      tcgtaaacc agtgcaaaa caagtgagt tcagggaaa 
      gcaaagcgg tgaaattat ccgaactgg ggaaaaaca 
      taggcggtt ggagggagt atcttcagg agtgtcaga 
      tggcgcttt tgcgagaag ttaaagagc gaaataggg 
      gttggattg ttgtttcgg gccataatc cgagagtcg 
      aagtatcgt acaaaaaag tggccgatg gccacccgc 
      tccgccttt ggaaagttt ccgcagagg ttgaagcgc 
      ccccccttg aggtcttac acaggatta tacgcgaga 
      ggcatttgt gggaccctg agttacact cggctaaga 
      tagtgtcgc aaattgaca aaaactgcg aagtgttcc 
      aatacgtcc gccgcaaaa tcgtgccgc tgccgaatg 
      aggaatatt gcgttgtgc ggacgtgga ggaccgatc 
      tcccgaaag acagttgct tggttattg agacttcgg 
      ttgagcatc aggatgctg tccccattc ccaatactg 
      tcaggggca ttgtgttgg cttcccggt acctcattg 
      aaccttgcg gctctggac gctcgaaat tattgcatt 
      tgtcccgtg ttgactcaa tgggaaaaa caccggaat 
      ggtatcaaa aaaattacg gacatatat gtgccctct 
      ttgttgcta atattgtaa gttcgtttt accacgtcg 
      ctgcaggac cagaaagta aagtataaa accaaagat 
      gtacataat aatgttgct aaatctaat tatattttt 
      atatgaagt aatcaatat tattatatg aacatgttg 
      ccgttttac attatttgt tatggacag taattaagt 
      atctatgtt attttattt tttacttaa ttttttatt 
      ttatgaaac attcacatt agttcgttt ttctaatca 
      cgttaccgg gaaagctat acacaaata cagtggtgt 
      catatggtt agttaggta cagttgtgg tgcaagggt 
      ttgacgtac tatagcttt cagtgtaat atatttaac 
      aattttatg tccatttgt gcaagacct acactgggt 
      tcaaatcgt agtcaaaat gagactgaa aataacttg 
      aaaagttca gtatactta atcataaaa aattgcaat 
      aaatgctca gtgtagaac ctcatattt taattaagt 
      taccatatt catgtttat taaatataa atatattat 
      tgcatattt atcagctgg atgagaaca ggcttgtca 
      gtgtttgtg ctagtcata taaattgtt tttaatctt 
      actagataa tacttttcc attaatgga ggccagtat 
      tgtattttt tatgttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY