Status
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Gene information
General information
TC031560
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004617,TC004618
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6544149..6550086
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031560
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06612

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031560 RA
      MSRADTYHL LIRLYHYET LRAFAPPSG FNLAPEVPS 
      SLQTTSRAS ALSRSNSGG EEKTHSDIH SSLDTIHLR 
      SRPASLVDE GLLLSYGQR NAFRNECKD YESFSSRHY 
      SHLDEHAWR YRHQIPSEH YQPDYGQCD EFRTRYPFR 
      EPETNRLDY LSLSLSPPT RQKESFYAH ESSAFTPSK 
      NVEDMNEDD LNRIVASEN NCSSSQEDP TSYDIGGYM 
      PIAEGDNLD DKYEVCRKL GYGQFSTVW LCQNKLNDE 
      FVAIKVSKS QSKLRALAQ DEIKLLDCA ILANPDHAG 
      YKNIVQMFD FFSCQSVNG NHTAIVLEV MGPSLLHLI 
      KQSEYRGIQ LPGVRRIIK QVLQGLQYL HEECGIIHT 
      DLKPENILI KAKEPYIRQ MVNTAKRFS ELGIIPPKT 
      YVTSNRWSD VQPYSKDLE EYERAQLLR TRSYPQDPF 
      LSEIVSFRR KHAEPKLKG PMWIDANIE VKIGDMGNA 
      TWVNNKYNS TIQTRQYRA LEVILDAGY DCPADVWSV 
      GCLAFELAT GEFLFYPKM YNNFSLDVD HITLIWEVL 
      GGIPTYITK RGSKADTFF SNGKLKHIE ESELKIWKI 
      EDVLVDKYK WKRLDAIPF AGFIEYLIE PDPSLRYTA 
      SAALSCEWI NEQ
    CDS sequence
    • >TC031560 RA
      atgtcgcgg gccgataca tatcacctg ctaatccgc 
      ctctaccat tatgagacc cttcgtgcg ttcgcgccc 
      ccttcaggc ttcaacctg gctccagag gtgcccagc 
      agtctccag acgacatct cgggctagt gcgctttca 
      aggtcgaac agcggcggc gaggagaaa acccattcc 
      gacattcac tcctcactg gacaccatc cacctcagg 
      tctcggcct gcctcgctg gtggacgaa ggactcctc 
      ctctcctat ggccaaagg aacgccttc aggaacgag 
      tgcaaggac tacgagtcc ttttcctca cggcactac 
      tcgcatctc gacgaacac gcctggaga tacagacac 
      caaattccg tccgaacat taccaacca gactatgga 
      cagtgtgat gagttcaga actcgatac ccattccgc 
      gaacctgaa acgaatcgc cttgactac ctctcgttg 
      tcgctttct ccgccgacg aggcagaag gagtccttc 
      tatgctcac gaatcttcc gctttcacg ccatcgaaa 
      aatgtagaa gatatgaac gaagatgac ttgaaccgg 
      attgtcgca agtgagaat aactgcagt tcgtcccaa 
      gaagacccc acgagctac gacatcggt ggttacatg 
      cccatcgcc gaaggcgac aacttggac gacaagtac 
      gaagtctgt cggaaattg ggctacggg cagttttcc 
      acagtctgg ctttgtcaa aataaacta aatgacgag 
      tttgtggcc atcaaagtg tccaagtcc cagtcaaaa 
      ctacgagcg ctggcccaa gacgaaata aaattgcta 
      gattgcgcg attttggca aaccctgat cacgccggt 
      tataagaat atagtacaa atgtttgac tttttttct 
      tgtcaaagt gttaatggc aatcacact gccattgtc 
      ttagaagtg atgggacca tcattacta catttaata 
      aaacaaagc gaatataga ggcattcaa cttccggga 
      gttcggcga ataataaaa caagtcctg caaggtcta 
      caatatctt cacgaggag tgcggcata atccatacc 
      gacttaaag ccggaaaat atcctaatt aaggccaaa 
      gaaccgtac attcgtcaa atggtaaac actgcgaaa 
      agattcagc gaattaggg atcattcct ccgaaaaca 
      tacgttacg tcaaaccgt tggagcgac gtgcagcct 
      tacagcaag gacctcgag gagtacgaa agagcccag 
      ctcttgcgc acgcggtct taccctcag gacccgttc 
      ctgtccgag atagtgagt ttcaggagg aagcacgct 
      gagcctaaa ctgaaggga ccaatgtgg atcgacgca 
      aatatcgag gtcaagatc ggcgacatg ggaaatgcg 
      acctgggtt aacaacaag tacaactcg accatccag 
      acccggcaa taccgcgcg ttggaggtg atcctggac 
      gcgggctac gactgtccc gccgacgtc tggtctgtg 
      ggctgcctg gcgttcgag ttggccacg ggcgagttc 
      ctgttctac cccaagatg tataataac ttctcgttg 
      gatgtggac cacattact ttaatttgg gaggtgctc 
      gggggcatt cccacgtat ataaccaag aggggttcc 
      aaagccgac acgttcttc tccaacggc aaactgaag 
      cacatagaa gagagcgag ctgaagatt tggaagatt 
      gaggacgtg cttgtcgac aagtacaag tggaagcgt 
      ctcgatgcg attccgttt gccggcttc attgagtat 
      ttaatcgaa cctgaccca agtttgaga tatactgct 
      tcagcggca ctcagctgc gaatggatt aatgaacaa 
      tga
    mRNA sequence
    • >TC031560 RA
      caagattgt aatcgtcat tggatcgtc gatgcgcga 
      agggcggtg ttcgccctg ggggtgaga aagtgtggt 
      catagtaat cgggttaaa aatcgggat ttgcaagag 
      tgaagacag ttgtggttt cagtaatca atttaattt 
      ccgtatttc gggtcgatt atcgaacgg tctctccag 
      gtcgcacat gcgctcggg gcgcctccc agtagagtc 
      gttagtgaa gtgctggaa gccgtaatc cccatgtcg 
      cgggccgat acatatcac ctgctaatc cgcctctac 
      cattatgag acccttcgt gcgttcgcg cccccttca 
      ggcttcaac ctggctcca gaggtgccc agcagtctc 
      cagacgaca tctcgggct agtgcgctt tcaaggtcg 
      aacagcggc ggcgaggag aaaacccat tccgacatt 
      cactcctca ctggacacc atccacctc aggtctcgg 
      cctgcctcg ctggtggac gaaggactc ctcctctcc 
      tatggccaa aggaacgcc ttcaggaac gagtgcaag 
      gactacgag tccttttcc tcacggcac tactcgcat 
      ctcgacgaa cacgcctgg agatacaga caccaaatt 
      ccgtccgaa cattaccaa ccagactat ggacagtgt 
      gatgagttc agaactcga tacccattc cgcgaacct 
      gaaacgaat cgccttgac tacctctcg ttgtcgctt 
      tctccgccg acgaggcag aaggagtcc ttctatgct 
      cacgaatct tccgctttc acgccatcg aaaaatgta 
      gaagatatg aacgaagat gacttgaac cggattgtc 
      gcaagtgag aataactgc agttcgtcc caagaagac 
      cccacgagc tacgacatc ggtggttac atgcccatc 
      gccgaaggc gacaacttg gacgacaag tacgaagtc 
      tgtcggaaa ttgggctac gggcagttt tccacagtc 
      tggctttgt caaaataaa ctaaatgac gagtttgtg 
      gccatcaaa gtgtccaag tcccagtca aaactacga 
      gcgctggcc caagacgaa ataaaattg ctagattgc 
      gcgattttg gcaaaccct gatcacgcc ggttataag 
      aatatagta caaatgttt gactttttt tcttgtcaa 
      agtgttaat ggcaatcac actgccatt gtcttagaa 
      gtgatggga ccatcatta ctacattta ataaaacaa 
      agcgaatat agaggcatt caacttccg ggagttcgg 
      cgaataata aaacaagtc ctgcaaggt ctacaatat 
      cttcacgag gagtgcggc ataatccat accgactta 
      aagccggaa aatatccta attaaggcc aaagaaccg 
      tacattcgt caaatggta aacactgcg aaaagattc 
      agcgaatta gggatcatt cctccgaaa acatacgtt 
      acgtcaaac cgttggagc gacgtgcag ccttacagc 
      aaggacctc gaggagtac gaaagagcc cagctcttg 
      cgcacgcgg tcttaccct caggacccg ttcctgtcc 
      gagatagtg agtttcagg aggaagcac gctgagcct 
      aaactgaag ggaccaatg tggatcgac gcaaatatc 
      gaggtcaag atcggcgac atgggaaat gcgacctgg 
      gttaacaac aagtacaac tcgaccatc cagacccgg 
      caataccgc gcgttggag gtgatcctg gacgcgggc 
      tacgactgt cccgccgac gtctggtct gtgggctgc 
      ctggcgttc gagttggcc acgggcgag ttcctgttc 
      taccccaag atgtataat aacttctcg ttggatgtg 
      gaccacatt actttaatt tgggaggtg ctcgggggc 
      attcccacg tatataacc aagaggggt tccaaagcc 
      gacacgttc ttctccaac ggcaaactg aagcacata 
      gaagagagc gagctgaag atttggaag attgaggac 
      gtgcttgtc gacaagtac aagtggaag cgtctcgat 
      gcgattccg tttgccggc ttcattgag tatttaatc 
      gaacctgac ccaagtttg agatatact gcttcagcg 
      gcactcagc tgcgaatgg attaatgaa caatgattt 
      attccaatc atgtatctt tactgttag aagactgat 
      cattttcgt tcgatttcg tttctagtt ttgttgtat 
      tctgtcatt tgtaataaa agttcaaaa tattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY