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Gene information
General information
TC031547
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010888
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4930875..4933074
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031547
    iBeetle screen iB_06610

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031547 RA
      MTKSSIFQK YCEKAIGTL TEEDVEKFK STTRDEERI 
      RMLYGVAQA VPITPINNG KDLKQAQEA KISGNKLFA 
      AKKYEEALH AYNEGIVVC PQDTDDGRE LLTILISNR 
      SAVFFEQEH FRKVFDDID YVIAVGNYP PKLHYKIWL 
      RKAKCYDAL QNEKYAEET YNLAISSLK HAELDEKSR 
      EKKIAEIQE SRKKKRKAC PDKNQIIPI SNADLFANG 
      NREYVAAHK NVYFDFDPI LGRFARALE DFDTGVIIV 
      EETPHCAVI SQENALMNC QFCCISTQQ PVACRNCGH 
      AVFCSLNCE RQANLTFHK YECKAQPVL FHAGASINC 
      AMALRMISQ KPHGFFQQK KKLLKDFLK DNCKKVPIK 
      SQIYRSDDY NAAFFLCRN EHLRKKGEL VHYSVMAIY 
      LLRLLKFSG YFGGNIKDD VVTEEEVFI ASLILRHLQ 
      ILQFNSHEI SELRNLNEE MVTNGIQCH YKSEYIGAG 
      LYPTLALFN HSCDPSIVR YNIGNRMIV RTIKPIKAG 
      EIIYENYGP LYTSMDADE RRVTLQNRY WFECYCTPC 
      QQEWPLFEY MDPNQIKIG CQKENCPFE FTLYKDDLC 
      PYFQCDYCD GVTKIFPSL KGLSQLAIM LPKAEDLYS 
      AGETREAMK LFMQSLDIL YKYSRPPCP DMIKVQQRL 
      KTLFVHLGN KQYNYVQKL
    CDS sequence
    • >TC031547 RA
      atgacgaaa agtagcatt ttccaaaag tactgtgag 
      aaagcaatt ggcacttta acggaggaa gatgtcgag 
      aaattcaaa tcaacaaca cgtgacgaa gaacgcatt 
      agaatgctg tacggtgtt gcccaagcg gtcccaatc 
      acgccgatt aacaacggc aaggacctc aaacaagcc 
      caggaggcg aaaatcagc ggaaacaaa ctgttcgcc 
      gccaaaaaa tacgaagaa gcgttgcat gcatacaat 
      gaggggata gttgtttgt ccgcaggac acagacgac 
      ggtagggaa ttgctgaca attttgatt tccaatcgc 
      agcgcagtt tttttcgag caggaacac ttccggaag 
      gttttcgac gatattgat tacgttatc gccgtcggt 
      aattacccc ccgaaactg cactataaa atttggttg 
      aggaaagcc aaatgttac gacgcgttg cagaatgag 
      aaatacgcg gaggagact tataattta gccatctcg 
      agcttgaaa catgccgag cttgacgaa aaaagccgc 
      gagaaaaaa atcgcagaa atacaagag tcacgcaag 
      aagaagagg aaagcgtgt ccagacaaa aaccaaatt 
      atcccaatt tccaacgcg gatcttttc gcgaacggg 
      aacagggag tatgtggcc gcacataag aacgtttat 
      ttcgatttt gacccaatc ttggggcgg tttgcacgc 
      gccttggag gattttgac acgggtgtt ataattgtc 
      gaggaaact ccgcactgt gctgtcatt tcccaggaa 
      aatgcctta atgaattgc cagttttgt tgtatttcc 
      acgcaacag cccgttgcg tgtcgtaat tgcggtcac 
      gcggttttt tgcagtctc aattgcgaa agacaagcg 
      aatttaact tttcacaaa tacgagtgt aaagctcaa 
      ccggtgttg tttcacgcc ggagcctcg atcaattgc 
      gcgatggct ctgcggatg atctcgcag aagccgcac 
      gggtttttc caacaaaag aaaaagctg ctgaaggat 
      tttttgaaa gacaattgc aagaaagtg ccaatcaag 
      agtcagatc tatcgatcg gatgattat aatgccgcg 
      tttttcctc tgtcggaat gaacacttg aggaaaaaa 
      ggggaattg gtgcattat tccgtaatg gcgatttat 
      ttgttgcgg cttttgaaa ttctcgggc tattttggg 
      gggaatatc aaggatgat gttgtcaca gaagaagag 
      gtttttatt gcgagttta attctacga cacttgcaa 
      attcttcaa tttaactcg cacgaaata tccgagctt 
      agaaatttg aacgaagaa atggtaact aacggaata 
      cagtgtcac tacaagagc gaatacata ggagctggg 
      ttgtaccca actttggca ctttttaac cactcctgt 
      gacccaagt atagttagg tataacata gggaatcga 
      atgattgta cgcacaata aagccaatc aaagccgga 
      gagataatt tacgaaaac tatggccct ttgtacact 
      tccatggac gcagacgaa aggagagta accctccaa 
      aatcgctat tggtttgaa tgttactgc actccttgc 
      caacaagaa tggccgctc ttcgaatat atggaccct 
      aatcagatc aaaatcggg tgccaaaaa gaaaattgc 
      ccattcgaa ttcacttta tacaaagac gacttatgt 
      ccttacttc cagtgtgac tattgcgac ggtgtcact 
      aaaattttc ccttccttg aaaggtctt tcacaactg 
      gcaataatg ttaccaaaa gctgaagat ttatacagt 
      gctggtgaa acacgtgaa gctatgaag ctttttatg 
      caatccttg gacatactg tataaatat tcaagacct 
      ccgtgtccc gacatgata aaggttcag caacgtttg 
      aagacactt ttcgtccat ttggggaac aaacaatat 
      aattatgta caaaaattg taa
    mRNA sequence
    • >TC031547 RA
      caattggct taaaataga acgctggcg tctgaaaat 
      aaacaaccg gcacccgtc catttagtt cgcgtgttc 
      tttttcgac atgacgaaa agtagcatt ttccaaaag 
      tactgtgag aaagcaatt ggcacttta acggaggaa 
      gatgtcgag aaattcaaa tcaacaaca cgtgacgaa 
      gaacgcatt agaatgctg tacggtgtt gcccaagcg 
      gtcccaatc acgccgatt aacaacggc aaggacctc 
      aaacaagcc caggaggcg aaaatcagc ggaaacaaa 
      ctgttcgcc gccaaaaaa tacgaagaa gcgttgcat 
      gcatacaat gaggggata gttgtttgt ccgcaggac 
      acagacgac ggtagggaa ttgctgaca attttgatt 
      tccaatcgc agcgcagtt tttttcgag caggaacac 
      ttccggaag gttttcgac gatattgat tacgttatc 
      gccgtcggt aattacccc ccgaaactg cactataaa 
      atttggttg aggaaagcc aaatgttac gacgcgttg 
      cagaatgag aaatacgcg gaggagact tataattta 
      gccatctcg agcttgaaa catgccgag cttgacgaa 
      aaaagccgc gagaaaaaa atcgcagaa atacaagag 
      tcacgcaag aagaagagg aaagcgtgt ccagacaaa 
      aaccaaatt atcccaatt tccaacgcg gatcttttc 
      gcgaacggg aacagggag tatgtggcc gcacataag 
      aacgtttat ttcgatttt gacccaatc ttggggcgg 
      tttgcacgc gccttggag gattttgac acgggtgtt 
      ataattgtc gaggaaact ccgcactgt gctgtcatt 
      tcccaggaa aatgcctta atgaattgc cagttttgt 
      tgtatttcc acgcaacag cccgttgcg tgtcgtaat 
      tgcggtcac gcggttttt tgcagtctc aattgcgaa 
      agacaagcg aatttaact tttcacaaa tacgagtgt 
      aaagctcaa ccggtgttg tttcacgcc ggagcctcg 
      atcaattgc gcgatggct ctgcggatg atctcgcag 
      aagccgcac gggtttttc caacaaaag aaaaagctg 
      ctgaaggat tttttgaaa gacaattgc aagaaagtg 
      ccaatcaag agtcagatc tatcgatcg gatgattat 
      aatgccgcg tttttcctc tgtcggaat gaacacttg 
      aggaaaaaa ggggaattg gtgcattat tccgtaatg 
      gcgatttat ttgttgcgg cttttgaaa ttctcgggc 
      tattttggg gggaatatc aaggatgat gttgtcaca 
      gaagaagag gtttttatt gcgagttta attctacga 
      cacttgcaa attcttcaa tttaactcg cacgaaata 
      tccgagctt agaaatttg aacgaagaa atggtaact 
      aacggaata cagtgtcac tacaagagc gaatacata 
      ggagctggg ttgtaccca actttggca ctttttaac 
      cactcctgt gacccaagt atagttagg tataacata 
      gggaatcga atgattgta cgcacaata aagccaatc 
      aaagccgga gagataatt tacgaaaac tatggccct 
      ttgtacact tccatggac gcagacgaa aggagagta 
      accctccaa aatcgctat tggtttgaa tgttactgc 
      actccttgc caacaagaa tggccgctc ttcgaatat 
      atggaccct aatcagatc aaaatcggg tgccaaaaa 
      gaaaattgc ccattcgaa ttcacttta tacaaagac 
      gacttatgt ccttacttc cagtgtgac tattgcgac 
      ggtgtcact aaaattttc ccttccttg aaaggtctt 
      tcacaactg gcaataatg ttaccaaaa gctgaagat 
      ttatacagt gctggtgaa acacgtgaa gctatgaag 
      ctttttatg caatccttg gacatactg tataaatat 
      tcaagacct ccgtgtccc gacatgata aaggttcag 
      caacgtttg aagacactt ttcgtccat ttggggaac 
      aaacaatat aattatgta caaaaattg taattaagc 
      gaataaagt tatcagttt ctc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY