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Gene information
General information
TC010881 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4977525..4979744
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010881
    iBeetle screen iB_06608

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber partiallynumber increased - Number of animals showing the phenotype: 3

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010881 RA
      MEGSRNPDH DKTSFWKKN SKTVPGRPS PKKDVKSSS 
      KPKMVGSTS FQDFQASVS DAWAMDDDD FCIISGLEN 
      AKISKKVSQ SAALNVLNS HRNSTDTPN SNEKPEAGE 
      VDHVRNSRI PGRPQQLRC LHPPQDEDS ESKLERFES 
      VLANPPSLQ ELCKLSWSG IPVKVRAIT WRLLSGYLP 
      INLERRNGV LERKRQDYW NLVDKYYYA EHDETNRDI 
      QHQINIDVP RMNPTIPLF QQKTVQIMF ERILFIWSI 
      RHPASGYVQ GINDLVTPF YVVFLQEFI LDNQAFETF 
      QVDTLSEEQ LRIIEADSF WCLSKFLDG IQDNYVFAQ 
      VGIQRKVHR LEELIKRVD ETLHRHLKQ HNVSYLQFS 
      FRWLNNLLT RELPLRCTI RLWDTYLAE NDCFASFQL 
      YVCASFLLY WKEDLMRQN DFQGLLLLL QNLPTQSWT 
      SSQISILVA EAYKLKVMF ADAPNHLQS NTGDS
    CDS sequence
    • >TC010881 RA
      atggaaggg tccaggaat ccggaccat gataaaacc 
      tcattctgg aagaagaac agcaaaaca gtacctgga 
      cgaccaagc cccaagaaa gatgtgaaa agtagctca 
      aaaccaaaa atggtaggc tccacgtcg tttcaagac 
      ttccaagcc tccgtcagc gacgcctgg gccatggac 
      gacgacgat ttttgtata atatccgga ctagagaac 
      gcgaaaata tcgaagaaa gtgtcacaa tccgcagct 
      ctaaacgta ctaaacagc caccgcaac agcacagat 
      actccaaac tcgaatgaa aagccggaa gccggcgaa 
      gtggaccac gttaggaac agcagaatt ccgggcagg 
      ccccagcag ctgcgctgc ctgcacccg ccgcaggat 
      gaggacagt gaatctaag ctggaacgc tttgagagt 
      gtcttagcc aatcctccc agtctccag gagctgtgc 
      aagctgagc tggtcgggg atccccgtc aaggtgcgc 
      gccatcacc tggaggctg ctgtcgggg tatctgccc 
      ataaacctc gagcggcgg aacggagtc ctggagcgg 
      aaaagacag gactactgg aatctagtg gacaagtat 
      tactacgct gagcatgat gagactaat cgtgatatt 
      cagcaccaa atcaacatt gatgtgcca agaatgaac 
      ccgacgatc cccctgttt caacaaaaa actgtacaa 
      atcatgttt gagagaatt ctgttcatt tggtcgatc 
      agacatcct gcctcaggt tacgtccaa gggatcaac 
      gatttggtg actcctttt tacgtggtg ttccttcaa 
      gagtttatt ttggataat caagccttt gagacgttt 
      caagtcgat actttaagc gaagaacaa ctcagaatc 
      atcgaggct gactcgttt tggtgctta tccaagttt 
      ctagatggt attcaagat aattacgtt ttcgcccaa 
      gttggcata cagcgcaag gtgcatcgg ttagaagaa 
      ttaattaag cgagtggac gagacgctg catcgccac 
      ctcaaacaa cacaacgtt agttattta caattctcc 
      tttcgttgg ttgaacaat ttactcact agagagctt 
      ccgttaagg tgcactatc aggttgtgg gacacatac 
      ttggcagag aacgactgc ttcgcctcg tttcaactt 
      tacgtttgt gcttctttt ctcctctac tggaaagag 
      gatctaatg cgccaaaac gattttcaa ggactgctc 
      ttactactt caaaatctt cctactcag tcttggaca 
      agttctcaa attagtatt ttagtggca gaggcatac 
      aaattgaaa gttatgttc gcagatgct ccaaaccat 
      ttgcaaagt aacactggt gatagctga
    mRNA sequence
    • >TC010881 RA
      cctgggtgc gaacgtcat ttttatgga atttttcca 
      aattggatc aaaatagaa acgttgatt tggcaacag 
      acatgttta ataaataat atcaaacat tgaaacaat 
      cacaacaaa tcaagatta gtttatttt ataaaataa 
      agccacata tctacagct acaaagata acgtaaact 
      acattaaac taaagctta agtgtaagc agttaattg 
      taaagcgaa aacaaagag aaacacttc aattaatta 
      catacaatt gatgtaaat aagcggaac tacctgtct 
      tcacaacat ggtggttat gacacgtga agcgccaaa 
      aacctaggg tgttttttt cagaaccga aaagtcttg 
      aaaataatt ccgtaatcc gtgtatttt tcggtaata 
      aagcctaat taggatcat cccaattaa aatacaaga 
      gaataactt taattaaaa ctggtgacg taatgtatt 
      ttgacgttg ccatgtttg ttgacagtt gggtaaaat 
      aacctcaaa gttggggcc ttgtcagag tgaaaatta 
      tggataact caccgaaaa gtgcgaaaa acgtgtaag 
      aatggaagg gtccaggaa tccggacca tgataaaac 
      ctcattctg gaagaagaa cagcaaaac agtacctgg 
      acgaccaag ccccaagaa agatgtgaa aagtagctc 
      aaaaccaaa aatggtagg ctccacgtc gtttcaaga 
      cttccaagc ctccgtcag cgacgcctg ggccatgga 
      cgacgacga tttttgtat aatatccgg actagagaa 
      cgcgaaaat atcgaagaa agtgtcaca atccgcagc 
      tctaaacgt actaaacag ccaccgcaa cagcacaga 
      tactccaaa ctcgaatga aaagccgga agccggcga 
      agtggacca cgttaggaa cagcagaat tccgggcag 
      gccccagca gctgcgctg cctgcaccc gccgcagga 
      tgaggacag tgaatctaa gctggaacg ctttgagag 
      tgtcttagc caatcctcc cagtctcca ggagctgtg 
      caagctgag ctggtcggg gatccccgt caaggtgcg 
      cgccatcac ctggaggct gctgtcggg gtatctgcc 
      cataaacct cgagcggcg gaacggagt cctggagcg 
      gaaaagaca ggactactg gaatctagt ggacaagta 
      ttactacgc tgagcatga tgagactaa tcgtgatat 
      tcagcacca aatcaacat tgatgtgcc aagaatgaa 
      cccgacgat ccccctgtt tcaacaaaa aactgtaca 
      aatcatgtt tgagagaat tctgttcat ttggtcgat 
      cagacatcc tgcctcagg ttacgtcca agggatcaa 
      cgatttggt gactccttt ttacgtggt gttccttca 
      agagtttat tttggataa tcaagcctt tgagacgtt 
      tcaagtcga tactttaag cgaagaaca actcagaat 
      catcgaggc tgactcgtt ttggtgctt atccaagtt 
      tctagatgg tattcaaga taattacgt tttcgccca 
      agttggcat acagcgcaa ggtgcatcg gttagaaga 
      attaattaa gcgagtgga cgagacgct gcatcgcca 
      cctcaaaca acacaacgt tagttattt acaattctc 
      ctttcgttg gttgaacaa tttactcac tagagagct 
      tccgttaag gtgcactat caggttgtg ggacacata 
      cttggcaga gaacgactg cttcgcctc gtttcaact 
      ttacgtttg tgcttcttt tctcctcta ctggaaaga 
      ggatctaat gcgccaaaa cgattttca aggactgct 
      cttactact tcaaaatct tcctactca gtcttggac 
      aagttctca aattagtat tttagtggc agaggcata 
      caaattgaa agttatgtt cgcagatgc tccaaacca 
      tttgcaaag taacactgg tgatagctg attaaatac 
      ttgtaataa ctgaatgtt cttttataa atgttttga 
      aacaaaact cttacattt gttgttgta aatacatga 
      cttgacaca tatttttat attgtatat gtgatttga 
      atcagttac tcaataaaa ccattaaat tacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY