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Gene information
General information
TC000589 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9349209..9357296
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000589
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06604

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03049

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000589 RA
      MDQQFCLRW NNHPTNLTD VLSSLLRRE ALCDVTLAC 
      DGETFKAHQ TILSACSPY FETIFIQNA HPHPIVFLK 
      DVNYNEMKA LLDFMYKGE VNVSQNLLP MFLKTAEAL 
      QIRGLTDNN SLSSKNDDV AEVPRREKE RIERDSPPI 
      EKRKRKSSS NCDNMTSNS ISERYNDSQ ASSQSNYKA 
      SPTLPKLNP ISPADRENM SAEDMRMSS PHIKQEPGH 
      DSSPHVDSF HTMSEALQT TNTWGRDVV LEVLPSTIQ 
      NKHNFTSTS QQCSVIQTI ASQKKSNVG RKGSRFRST 
      WMESYYWLQ YDEHENLMY CKFCRKWSA EIPEIRTSF 
      AEGSTNFRL EIVNHHDKC KAHRLCVAK EGGSVSVHP 
      EDIKLLGPH SLSDQTESD GTPPIPPPE ATDTIDAAW 
      QEQGQAYHP SSFYPQESQ IPTMVVETR APASDYCEM 
      FDKISDSPS LYTCKICGK TVSNRWHHS SIHRPQSNR 
      CPLCQQSFT RKDNMKAHI RLKHGRPLV LFETLGDNM 
      YS
    • >TC000589 RB
      MDQQFCLRW NNHPTNLTD VLSSLLRRE ALCDVTLAC 
      DGETFKAHQ TILSACSPY FETIFIQNA HPHPIVFLK 
      DVNYNEMKA LLDFMYKGE VNVSQNLLP MFLKTAEAL 
      QIRGLTDNN SLSSKNDDV AEVPRREKE RIERDSPPI 
      EKRKRKSSS NCDNMTSNS ISERYNDSQ ASSQSNYKA 
      SPTLPKLNP ISPADRENM SAEDMRMSS PHIKQEPGH 
      DSSPHVDSF HTMSEALQT GGSVSVHPE DIKLLGPHS 
      LSDQTESDG TPPIPPPEA TDTIDGSKT WHMRLTFDK 
      LPGGCNLHR CKLCGKIVT HIRNHYHVH FPGRFECPL 
      CRATYTRSD NLRTHCKFK HPRYNPDTR KFEAPTITN 
      DTTAD
    CDS sequence
    • >TC000589 RA
      atggatcaa cagttctgc ctgcgttgg aacaaccac 
      cccacgaac ctcaccgac gtgctgagc agcctcctg 
      cggcgggag gccctctgc gatgtgacg ctggcctgc 
      gacggggag accttcaag gcgcaccag accatcctg 
      tcggcgtgc agcccctat ttcgagacg atattcatc 
      cagaatgct cacccccat ccgatcgtt ttcctcaaa 
      gatgtcaat tacaacgaa atgaaagcg ctgctggat 
      ttcatgtat aaaggtgaa gtgaacgtc tcacagaac 
      ctcctaccg atgttccta aaaacggcc gaggccttg 
      cagattagg ggcttgacc gataataat tcgttaagt 
      agtaaaaac gacgacgta gctgaagtg ccaagaagg 
      gagaaggaa cggatcgag agggactcg ccgccgatt 
      gagaaacga aaacggaaa tcgtcttca aactgtgat 
      aatatgacg tcaaactca atttcagaa cgatataat 
      gactctcag gcatcatct caaagcaac tataaagca 
      agtccgacg ctgccaaag ttaaaccct atttcgccg 
      gccgataga gaaaatatg agcgctgaa gacatgagg 
      atgtcttcg ccgcatatt aaacaagaa ccggggcac 
      gactcaagc ccacacgtc gattcgttt catacaatg 
      tctgaagct ttacaaaca acgaacacg tgggggaga 
      gacgtggtc ctcgaagta ttaccatca acgattcaa 
      aataaacac aatttcaca tcgaccagt cagcagtgt 
      tcggtgatt cagacgata gcgtcgcaa aaaaaatcg 
      aacgtcggc aggaaagga agccggttc aggagcacg 
      tggatggaa tcgtactat tggttgcaa tacgacgaa 
      catgaaaat ctcatgtac tgcaaattt tgtcgaaaa 
      tggagcgct gagataccg gaaatacgg acgagtttt 
      gcggaaggg agtaccaat tttcgattg gaaatcgtc 
      aatcatcac gacaagtgc aaagcgcat agattgtgt 
      gtcgctaaa gaaggcgga agtgtaagt gtacacccg 
      gaagatatt aaattattg ggcccccat tcgttatca 
      gatcaaacc gaatcagat ggtactcca cccatacca 
      ccaccggaa gcaacagac actatagat gcagcgtgg 
      caggaacag ggacaggcc taccatcct tcatctttc 
      tacccgcaa gagtcgcaa attccgacg atggtcgtt 
      gaaacacgc gcgcccgca tccgattat tgcgagatg 
      tttgacaag atatcggat tcgcctagt ctatacacg 
      tgtaaaatt tgtggtaaa actgtgagc aatcgatgg 
      catcactcg tcgatacat cggccgcaa tcgaatcga 
      tgtccgttg tgtcagcag tctttcacg aggaaagac 
      aatatgaaa gcgcacatt cggctcaaa cacggacga 
      ccgttggtg cttttcgaa acattggga gataatatg 
      tattcgtga
    • >TC000589 RB
      atggatcaa cagttctgc ctgcgttgg aacaaccac 
      cccacgaac ctcaccgac gtgctgagc agcctcctg 
      cggcgggag gccctctgc gatgtgacg ctggcctgc 
      gacggggag accttcaag gcgcaccag accatcctg 
      tcggcgtgc agcccctat ttcgagacg atattcatc 
      cagaatgct cacccccat ccgatcgtt ttcctcaaa 
      gatgtcaat tacaacgaa atgaaagcg ctgctggat 
      ttcatgtat aaaggtgaa gtgaacgtc tcacagaac 
      ctcctaccg atgttccta aaaacggcc gaggccttg 
      cagattagg ggcttgacc gataataat tcgttaagt 
      agtaaaaac gacgacgta gctgaagtg ccaagaagg 
      gagaaggaa cggatcgag agggactcg ccgccgatt 
      gagaaacga aaacggaaa tcgtcttca aactgtgat 
      aatatgacg tcaaactca atttcagaa cgatataat 
      gactctcag gcatcatct caaagcaac tataaagca 
      agtccgacg ctgccaaag ttaaaccct atttcgccg 
      gccgataga gaaaatatg agcgctgaa gacatgagg 
      atgtcttcg ccgcatatt aaacaagaa ccggggcac 
      gactcaagc ccacacgtc gattcgttt catacaatg 
      tctgaagct ttacaaaca ggcggaagt gtaagtgta 
      cacccggaa gatattaaa ttattgggc ccccattcg 
      ttatcagat caaaccgaa tcagatggt actccaccc 
      ataccacca ccggaagca acagacact atagatggt 
      tcaaaaacc tggcacatg cggctaacg tttgacaag 
      ctgccaggt ggctgcaac ctgcaccgg tgcaaattg 
      tgtgggaaa atagtgacg cacatacgc aaccactat 
      catgtgcac ttcccgggc aggttcgag tgtcccctg 
      tgtcgagcc acgtataca cgcagcgac aatctgcgc 
      actcattgc aaattcaaa catccgcgc tacaatccc 
      gacaccagg aagttcgaa gcacccacg attaccaat 
      gacacgacg gccgattga
    mRNA sequence
    • >TC000589 RA
      ccgagatga ataattacg cgctctggt cgttcattt 
      ttaagccaa tgataatca aaaatcgtc aaatcggcc 
      attttccgc cccacaaat gccaataac ccagtaata 
      accccttgg ccggtcata aaataaata ttcccctcg 
      ccagttttt catccaact gacatcaaa acaaatata 
      acagtatca gacagtttg catggcagt gcaggaggt 
      agaaaattg ggtggcgtt gtaatgaca gaaactggg 
      caaaaaggc acttaaaat ggatcaaca gttctgcct 
      gcgttggaa caaccaccc cacgaacct caccgacgt 
      gctgagcag cctcctgcg gcgggaggc cctctgcga 
      tgtgacgct ggcctgcga cggggagac cttcaaggc 
      gcaccagac catcctgtc ggcgtgcag cccctattt 
      cgagacgat attcatcca gaatgctca cccccatcc 
      gatcgtttt cctcaaaga tgtcaatta caacgaaat 
      gaaagcgct gctggattt catgtataa aggtgaagt 
      gaacgtctc acagaacct cctaccgat gttcctaaa 
      aacggccga ggccttgca gattagggg cttgaccga 
      taataattc gttaagtag taaaaacga cgacgtagc 
      tgaagtgcc aagaaggga gaaggaacg gatcgagag 
      ggactcgcc gccgattga gaaacgaaa acggaaatc 
      gtcttcaaa ctgtgataa tatgacgtc aaactcaat 
      ttcagaacg atataatga ctctcaggc atcatctca 
      aagcaacta taaagcaag tccgacgct gccaaagtt 
      aaaccctat ttcgccggc cgatagaga aaatatgag 
      cgctgaaga catgaggat gtcttcgcc gcatattaa 
      acaagaacc ggggcacga ctcaagccc acacgtcga 
      ttcgtttca tacaatgtc tgaagcttt acaaacaac 
      gaacacgtg ggggagaga cgtggtcct cgaagtatt 
      accatcaac gattcaaaa taaacacaa tttcacatc 
      gaccagtca gcagtgttc ggtgattca gacgatagc 
      gtcgcaaaa aaaatcgaa cgtcggcag gaaaggaag 
      ccggttcag gagcacgtg gatggaatc gtactattg 
      gttgcaata cgacgaaca tgaaaatct catgtactg 
      caaattttg tcgaaaatg gagcgctga gataccgga 
      aatacggac gagttttgc ggaagggag taccaattt 
      tcgattgga aatcgtcaa tcatcacga caagtgcaa 
      agcgcatag attgtgtgt cgctaaaga aggcggaag 
      tgtaagtgt acacccgga agatattaa attattggg 
      cccccattc gttatcaga tcaaaccga atcagatgg 
      tactccacc cataccacc accggaagc aacagacac 
      tatagatgc agcgtggca ggaacaggg acaggccta 
      ccatccttc atctttcta cccgcaaga gtcgcaaat 
      tccgacgat ggtcgttga aacacgcgc gcccgcatc 
      cgattattg cgagatgtt tgacaagat atcggattc 
      gcctagtct atacacgtg taaaatttg tggtaaaac 
      tgtgagcaa tcgatggca tcactcgtc gatacatcg 
      gccgcaatc gaatcgatg tccgttgtg tcagcagtc 
      tttcacgag gaaagacaa tatgaaagc gcacattcg 
      gctcaaaca cggacgacc gttggtgct tttcgaaac 
      attgggaga taatatgta ttcgtgaga gttttcgat 
      gagcgaggt tttggctgt gagggggca aatctaacg 
      ccaaattcc tggtttggt ggtagattt gaacagttc 
      taggtttaa cagtattcg aagcgactc tttgtaatt 
      gtatacaat ttgtgattt atgtatcgt ttttactta 
      tattcaagt taccttttt tacttatgt tataatttg 
      cttttttat acaagacgc agtgatgta tttaatgat 
      atctaatca aacagtaat tattgtaaa cgttacttt 
      atgtaatcg taataaaga agagattat gct
    • >TC000589 RB
      ccgagatga ataattacg cgctctggt cgttcattt 
      ttaagccaa tgataatca aaaatcgtc aaatcggcc 
      attttccgc cccacaaat gccaataac ccagtaata 
      accccttgg ccggtcata aaataaata ttcccctcg 
      ccagttttt catccaact gacatcaaa acaaatata 
      acagtatca gacagtttg catggcagt gcaggaggt 
      agaaaattg ggtggcgtt gtaatgaca gaaactggg 
      caaaaaggc acttaaaat ggatcaaca gttctgcct 
      gcgttggaa caaccaccc cacgaacct caccgacgt 
      gctgagcag cctcctgcg gcgggaggc cctctgcga 
      tgtgacgct ggcctgcga cggggagac cttcaaggc 
      gcaccagac catcctgtc ggcgtgcag cccctattt 
      cgagacgat attcatcca gaatgctca cccccatcc 
      gatcgtttt cctcaaaga tgtcaatta caacgaaat 
      gaaagcgct gctggattt catgtataa aggtgaagt 
      gaacgtctc acagaacct cctaccgat gttcctaaa 
      aacggccga ggccttgca gattagggg cttgaccga 
      taataattc gttaagtag taaaaacga cgacgtagc 
      tgaagtgcc aagaaggga gaaggaacg gatcgagag 
      ggactcgcc gccgattga gaaacgaaa acggaaatc 
      gtcttcaaa ctgtgataa tatgacgtc aaactcaat 
      ttcagaacg atataatga ctctcaggc atcatctca 
      aagcaacta taaagcaag tccgacgct gccaaagtt 
      aaaccctat ttcgccggc cgatagaga aaatatgag 
      cgctgaaga catgaggat gtcttcgcc gcatattaa 
      acaagaacc ggggcacga ctcaagccc acacgtcga 
      ttcgtttca tacaatgtc tgaagcttt acaaacagg 
      cggaagtgt aagtgtaca cccggaaga tattaaatt 
      attgggccc ccattcgtt atcagatca aaccgaatc 
      agatggtac tccacccat accaccacc ggaagcaac 
      agacactat agatggttc aaaaacctg gcacatgcg 
      gctaacgtt tgacaagct gccaggtgg ctgcaacct 
      gcaccggtg caaattgtg tgggaaaat agtgacgca 
      catacgcaa ccactatca tgtgcactt cccgggcag 
      gttcgagtg tcccctgtg tcgagccac gtatacacg 
      cagcgacaa tctgcgcac tcattgcaa attcaaaca 
      tccgcgcta caatcccga caccaggaa gttcgaagc 
      acccacgat taccaatga cacgacggc cgattgatt 
      ttggctcta ctattatct gttgtacta cagggcctt 
      ggcctcatc ctagccacc aacgttcta ttctagcta 
      attcaagta ccttattaa aacaaatgc attgagaaa 
      aaaacgggc agtcaccga actgccccc tatctacaa 
      tactaaaga cttaaatta ttgcacagg ataatttcc 
      ttggaccat gtatttcta gtgtttcat ttcttatta 
      attcgagct acgactgaa atctcggca aggtattat 
      taaatgcaa tgtaggaga gaaataacg gtactatat 
      tcaaactat tcatgctaa aatccattt caaaatgcc 
      caccctcga tcaaagcgc ttgatcacc tccaaagtt 
      aaatgtttc gttgaactt gtcagtgtt gccaacgtt 
      tctgtcgga caatttgaa atgatttta gtctagtat 
      atagatagt ctttttaac atttttgta ccattttcg 
      tgcaagcat attttaaaa cctgtcacc gtttgaacc 
      tttactttc gaccagtaa cttctagtc tcgtaggtc 
      acaaaaaca atccgtact tccggcaat ttaaaatgt 
      gtttgcatc taaatgatg tcatcctgc aaaatatta 
      cgacctaag attgtaaag atatctgtg atgttatat 
      atgatgtaa tatacaggg ttccaagca aggctgacc 
      tactgtact taggaatca aaattgccc acttatact 
      cacaattgt tacggtagc ttagttttg tatggaaca 
      tggattgaa aaaatttta gaaactttg tgcaatcgt 
      tttaaaatt ttctattta tagtggcat taagtaccc 
      ggcgatccc ctgtatatg tataactta atttaaaaa 
      tgataaaac gaataaaaa aagagactc acgatatta 
      taatagctt atgcccgtc cgtttaaaa tgggtttgc 
      attttagga ttttcaatg tagagcgat tacaagtca 
      aatcttttt tgtaaaact ttatttatc ttatttaaa 
      tattgccaa actaactag ttgtataat ttaagtgca 
      attttatta aataacttt gatattacc tcatattct 
      caactttta catttaatg tagagaatc tctttttta 
      aattttgta cctattgta aggtattgg cggcctttg 
      aatagttaa ttatgtaag taaaatatt aactaattt 
      taggattat ttgtttgtg gaacaaatt atttaatga 
      tctgtagac gaaaaactc aaaaacttc tgtccgtaa 
      tttttacag gaatggatc atttctatt gaatttcgt 
      gatatggta ccttagtta taataagtt ggttcaatt 
      tggtgttac aggtgtggt gtttaataa gggtatttg 
      ttttttttt aagaagatt tgcttataa tatattcac 
      aaatgttta ttttatgat ataaaccta gtgcattaa 
      cttaggtaa gagcatgtt tttttaatt ctttaaaaa 
      agcggaaca tttgttgtt agtttttat aataatata 
      ctatatact aaagatttc tatgatata ttggcatta 
      agataaaaa agttgggtg agcaactag aatgattgt 
      gataatata ggtttggtg ctaacaacc ttgtactat 
      ttgaaataa aatatgaaa tgtgcaata tttagttgt 
      aactgtttt gttgttgta ctatccttg aatttagaa 
      aaaaagaga atcatgtac agtttgtaa aaggcgtta 
      ttgtatatt gtggtttca taaaaataa ttttgttat 
      tgttgattt tagaattac aattcttgc aacgagtta 
      tttttataa ttaattgta aaaaatcga gttaataag 
      gttgcggtt tcggagaaa atcatttta taaatatat 
      attgtacgg ccctaatcg gctctaaaa gaacaattc 
      gcattttct ttacaaaaa ttgtctaat atgctcaat 
      gatcaaata tcttgctta atatcattt tcgccaccg 
      ttgagtgtt acttaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY