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Gene information
General information
TC010812 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5988525..5990009
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010812
      iBeetle screen iB_06601

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      remarks: eggs lost,
      Adult morphology
      remarks: eggs lost,
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: eggs lost,
      remarks - cuticles: later than schedule,
       
      8 days later, due to lost eggs in day 9
       

      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010812 RA
        MVFNTSQIN LNVVLLVLL GISNKVDFI 
        MAHQCYVCG PKANISCDK FDPTDKTFI 
        KECPSAQAC SIQTHGNLV ERSCEEIGI 
        RDCKTANNV QYCFCSENL CNGKTINFT 
        DDEDLNDEG SGVSVITNT IQKEHKTIH 
        TASQSSQVQ LYFCSTWIS ILAILCN
      CDS sequence
      • >TC010812 RA
        atggtattt aatacaagt caaataaat ctaaacgtt 
        gttttactc gtgctatta gggatttct aacaaggtc 
        gactttata atggcccat caatgctac gtctgtggc 
        cccaaagcc aacatcagc tgtgataaa ttcgatccc 
        accgataaa acatttatt aaagagtgt ccttcagcc 
        caagcctgc agcatccaa acacatggc aatttggtc 
        gaaaggtcg tgcgaagaa atcggaatt cgcgactgt 
        aaaacagcg aataacgtc cagtactgc ttctgctca 
        gaaaatttg tgtaacggc aagacaatc aatttcacc 
        gatgacgaa gacttgaac gacgaaggg agcggcgta 
        tctgtgata accaacacc attcaaaag gagcacaaa 
        acaatccac actgcaagt caatccagt caggtccag 
        ctttatttc tgcagcacc tggatctcc atcctagca 
        atactgtgc aattaa
      mRNA sequence
      • >TC010812 RA
        tcactgtaa taaaatatg tcaaacaga aacattgcg 
        agtgtagtg agtcgttat taagcctat ttttaatta 
        atctgaata aatttagca aagaagagg aggaacgga 
        ataaacagt tcagtgaat tctgtcccc cagttaacc 
        tcattaatt gtgttcatg tctgttgag tgcataaca 
        ctgcaaaaa taaatttgt ttaacaaat ttcgtgtgc 
        tatgtgtat aaaccgcat ttttaacaa ataacccaa 
        ccccgcttg cgacagttt tttaaaatg gtatttaat 
        acaagtcaa ataaatcta aacgttgtt ttactcgtg 
        ctattaggg atttctaac aaggtcgac tttataatg 
        gcccatcaa tgctacgtc tgtggcccc aaagccaac 
        atcagctgt gataaattc gatcccacc gataaaaca 
        tttattaaa gagtgtcct tcagcccaa gcctgcagc 
        atccaaaca catggcaat ttggtcgaa aggtcgtgc 
        gaagaaatc ggaattcgc gactgtaaa acagcgaat 
        aacgtccag tactgcttc tgctcagaa aatttgtgt 
        aacggcaag acaatcaat ttcaccgat gacgaagac 
        ttgaacgac gaagggagc ggcgtatct gtgataacc 
        aacaccatt caaaaggag cacaaaaca atccacact 
        gcaagtcaa tccagtcag gtccagctt tatttctgc 
        agcacctgg atctccatc ctagcaata ctgtgcaat 
        taagcgcaa cgtcggtat tatttcatc agtattaag 
        ttaagtctt ccaccaaag atcagtttc gtaattatt 
        ttatttatt ttttacgtt ctctgacac gtgtagtca 
        attactggc aacggttgt gctaaagag tattgcttg 
        aaattattt ttctgtgat tatgccgaa tcttgtcaa 
        ttctcacta cagtacaac cccttttac ccgaacagc 
        aagttgtat tccttacag attttgagt caatgccag 
        gcctcacgg ccaaacaac aaattaaaa atgtatttt 
        acttcgttt tttagacca tttactttt ccaataatt 
        gcaataatt agttcgggt gatgggtgt tttactgta 
        acctgtaac taaaatgtg attttgttt ttatgataa 
        gagaatcag tattttgga tatgaatat attacgctt 
        gtcagcttt tatataaac actaattta gccccactg 
        ctgctgaat tccagcggt atttgattt agatctagt 
        cattgtgaa gttatgtaa cgtcctctc agatattac 
        aaacatttt tacgcataa acacgcttt attctgaaa 
        ggacacaat tactccgat gtgacttaa ccaaaaatg 
        ctcaataaa ttgaaacta aagta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY