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Gene information
General information
TC010832 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6090201..6093594
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010832
    iBeetle screen iB_06600

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010832 RA
      MAEERVNLS SEPSIDYHI VTLFEKLEA LRNDKPNSQ 
      FTSKLSIKV EVRTLEFWR SVISECIAS FVYVFVVCG 
      AKAGAFDGG SATTSLGVL STALAAGFA MISLTQCFG 
      HISGAHINP AVSLAMGIT KNISLLRTT MFIVAQCGG 
      AIAGAAFLY GVSIPRTQE DLPMSLPIK DGPWERFGN 
      EFILTFVVV LAYFVSMDA QRRWMGMSA ITIGATYSA 
      CSFVSPMPH LNPARSLGP SFVLKKWDN HWVYWLGPI 
      IGGVAAGLI HEYIFNSKR SKRQNSSSD GDSSSIQSD 
      EDTYDDLDK PNAPKYQES TYNSYRAGA PNGPTGYCP 
      SLTSASLYS TPATKLERV ESLYGGTRS LYCKSPPLT 
      RANLNRSQS VYTKNSGGP RDILPKPGP LVPTQSLHS 
      IKLNQQTHV HNQNLQNQL QQRSESVYG VRGNPPGSL 
      SRPETEKSA YPSKNSAPN SEESKKNSK INRPESMYG 
      IMSLQRRGQ SAQSDDSSY GSYHGPAPR NNTYHSNTS 
      GFQNKAGSV ASYVSRNAG SAEGRPPPS ANQLLTTTG 
      NPTNSYHHH QHSPSSQY
    CDS sequence
    • >TC010832 RA
      atggcagag gaacgcgtt aatttatct agtgagccg 
      agcatagac taccacata gtgacactt ttcgaaaag 
      ttggaagct ttaagaaat gataagccg aactcgcag 
      tttacttca aaattgtca attaaagtc gaggtccgc 
      actttggaa ttttggaga tcggtcatc tccgagtgc 
      atagcgtct tttgtttac gtgtttgtg gtgtgcggt 
      gcaaaggca ggtgctttt gacggaggt tcggcaact 
      acctctttg ggagttctt tcaacagca ctggcagct 
      ggctttgca atgatatcg ttaacacag tgcttcgga 
      cacatctct ggggcgcac ataaacccg gccgtgagt 
      ctggccatg ggcattacg aagaatatt tcgcttctg 
      cggacgaca atgttcatt gtcgcacaa tgcgggggt 
      gcgatcgcc ggcgccgct ttcctctac ggcgtatcc 
      attccgcga actcaggag gatctcccg atgtccctg 
      ccgataaaa gatgggcct tgggagagg ttcgggaac 
      gagtttatt ctgactttc gtggtggtt ttggcctat 
      ttcgtctca atggacgcc caaaggagg tggatgggc 
      atgtcggcg attacgatc ggagccacg tactccgct 
      tgctcgttc gtttcgccg atgccgcac ttgaaccca 
      gcgcgttcg ctggggccg tcctttgtt ttgaaaaaa 
      tgggacaac cactgggtt tattggctg gggcccata 
      attggcggc gtggcagct gggttaatc cacgagtac 
      attttcaac tcgaagcgc tcgaaacgc caaaacagc 
      tcgagtgac ggcgactcg tccagcatc cagtcggat 
      gaggacact tacgacgac ctcgacaaa cccaatgcc 
      ccaaaatac caggaatcg acttacaac tcctacaga 
      gccggcgct ccgaacggc cccaccggt tattgtccg 
      agtttgaca tcggccagt ctatattca acacctgcc 
      acaaaatta gaacgggtt gagtctctc tacggaggg 
      acaaggtcg ctgtattgt aaatcgcct ccactcact 
      agagccaat ttaaaccgc tcgcagtcg gtttacacg 
      aaaaacagc ggcggccct cgggacatc ctcccgaag 
      cccggtccc ctggtcccc acgcagtcg ctacattcg 
      ataaaactg aaccaacaa acgcacgtt cataaccag 
      aacttgcaa aatcagctc caacagcgc tccgaaagc 
      gtgtatggg gtgagggga aacccgccg gggagcctc 
      tcgcggccc gaaaccgag aaaagcgcc tatcccagt 
      aaaaatagc gcgccgaat agtgaagag agcaaaaag 
      aactcgaag ataaacagg ccggagtcg atgtacggg 
      attatgagt ttgcagcgt aggggacag tcggcccag 
      tcggacgac tcgagctac gggtcctat catggacca 
      gctccgagg aacaacacg tatcattcc aacacttcg 
      ggtttccag aataaggcc ggaagcgtg gcgagttat 
      gtgagcagg aacgccggg tccgcggag gggagaccg 
      cccccttcg gcaaaccag ctcctcacc accacagga 
      aacccgacc aattcgtac catcaccac caacactcg 
      ccctcgtct caatattaa
    mRNA sequence
    • >TC010832 RA
      aagggatgg cggatccgc ttgtaattg gtgatgatc 
      ctgggaggg ggaattcac catgtggtc ggtcgaatg 
      caatgtgcg aagagaggt ttcttcgga aatgtcccg 
      tcggtccat cggcaccgt tctttaaat taaacctcg 
      tcccttttt ccaaaaatg aacacttag tgcaatatt 
      tcgagtatg gcagaggaa cgcgttaat ttatctagt 
      gagccgagc atagactac cacatagtg acacttttc 
      gaaaagttg gaagcttta agaaatgat aagccgaac 
      tcgcagttt acttcaaaa ttgtcaatt aaagtcgag 
      gtccgcact ttggaattt tggagatcg gtcatctcc 
      gagtgcata gcgtctttt gtttacgtg tttgtggtg 
      tgcggtgca aaggcaggt gcttttgac ggaggttcg 
      gcaactacc tctttggga gttctttca acagcactg 
      gcagctggc tttgcaatg atatcgtta acacagtgc 
      ttcggacac atctctggg gcgcacata aacccggcc 
      gtgagtctg gccatgggc attacgaag aatatttcg 
      cttctgcgg acgacaatg ttcattgtc gcacaatgc 
      gggggtgcg atcgccggc gccgctttc ctctacggc 
      gtatccatt ccgcgaact caggaggat ctcccgatg 
      tccctgccg ataaaagat gggccttgg gagaggttc 
      gggaacgag tttattctg actttcgtg gtggttttg 
      gcctatttc gtctcaatg gacgcccaa aggaggtgg 
      atgggcatg tcggcgatt acgatcgga gccacgtac 
      tccgcttgc tcgttcgtt tcgccgatg ccgcacttg 
      aacccagcg cgttcgctg gggccgtcc tttgttttg 
      aaaaaatgg gacaaccac tgggtttat tggctgggg 
      cccataatt ggcggcgtg gcagctggg ttaatccac 
      gagtacatt ttcaactcg aagcgctcg aaacgccaa 
      aacagctcg agtgacggc gactcgtcc agcatccag 
      tcggatgag gacacttac gacgacctc gacaaaccc 
      aatgcccca aaataccag gaatcgact tacaactcc 
      tacagagcc ggcgctccg aacggcccc accggttat 
      tgtccgagt ttgacatcg gccagtcta tattcaaca 
      cctgccaca aaattagaa cgggttgag tctctctac 
      ggagggaca aggtcgctg tattgtaaa tcgcctcca 
      ctcactaga gccaattta aaccgctcg cagtcggtt 
      tacacgaaa aacagcggc ggccctcgg gacatcctc 
      ccgaagccc ggtcccctg gtccccacg cagtcgcta 
      cattcgata aaactgaac caacaaacg cacgttcat 
      aaccagaac ttgcaaaat cagctccaa cagcgctcc 
      gaaagcgtg tatggggtg aggggaaac ccgccgggg 
      agcctctcg cggcccgaa accgagaaa agcgcctat 
      cccagtaaa aatagcgcg ccgaatagt gaagagagc 
      aaaaagaac tcgaagata aacaggccg gagtcgatg 
      tacgggatt atgagtttg cagcgtagg ggacagtcg 
      gcccagtcg gacgactcg agctacggg tcctatcat 
      ggaccagct ccgaggaac aacacgtat cattccaac 
      acttcgggt ttccagaat aaggccgga agcgtggcg 
      agttatgtg agcaggaac gccgggtcc gcggagggg 
      agaccgccc ccttcggca aaccagctc ctcaccacc 
      acaggaaac ccgaccaat tcgtaccat caccaccaa 
      cactcgccc tcgtctcaa tattaatac tgcgagcat 
      tgagattac cgtttgtac agcatttac aagtttcga 
      tgtatccaa agaatagcc gatagtttt ttagattag 
      gcttacgtg taagtaggt atatgtatt atggttata 
      ctttacgat aggtacata gaaaagtgt tcttgttta 
      gccagtagt ttatttata aacaggact gaaaccgac 
      atgtctcca ataaaacgt ttttgaaaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY