| General information | |||
| TC005185 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 4606265..4608714 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005185 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06598 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
remarks: eggs lost,
remarks - cuticles: later than schedule,
|
8 days later, due to lost eggs in day 9
|
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC005185 RA
MNPGAPNYP MASLYVGDL HTDITEAML FEKFSTAGP VLSIRVCRD LITRRSLGY AYVNFQQPA DAERALDTM NFDLIKGRP IRIMWSQRD PSLRKSGVG NVFIKNLDR SIDNKAMYD TFSAFGNIL SCKVAQDEN GTSKGYGFV HFETEEAAN KSIEKVNGM LLNGKKVYV GRFIPRKER EKELGEKAK LFTNVYVKN FGEDLTEEQ LRTMFEKYG KITSYKIMS KDDGKSKGF GFVAFESPE AAETAVEAL NGKEIIDGK PLYVGRAQK KAERQQELK RRFEALKME RLNRYQGVN LYVKNLDDT IDDERLRKE FSPFGTITS AKVMMEDNR SKGFGFVCF SSPEEATKA VTEMNGRIV GSKPLYVAL AQRKEDRKA HLTSQYMQR MANIRMHQM GQFIQPGTS SGYFVPTIP APQRFYGAA QMAQIRTNP RWPAQTPVR PGAQGSANP YGTIPTTYR AAPRPPNQS AALRSNINV PRPITGQQP PNIQGRPLA SQAVVAPAG LGISNAAPV QQAVHIQGQ EPLTATMLA AAPPQEQKQ MLGERLFPL IQRMYPDLA GKITGMLLE IDNSELLHM LEHNESLKA KVEEAVAVL QAHQAKQAA IKKE
- >TC005185 RB
MNPGAPNYP MASLYVGDL HTDITEAML FEKFSTAGP VLSIRVCRD LITRRSLGY AYVNFQQPA DAERALDTM NFDLIKGRP IRIMWSQRD PSLRKSGVG NVFIKNLDR SIDNKAMYD TFSAFGNIL SCKVAQDEN GTSKGYGFV HFETEEAAN KSIEKVNGM LLNGKKVYV GRFIPRKER EKELGEKAK LFTNVYVKN FGEDLTEEQ LRTMFEKYG KITSYKIMS KDDGKSKGF GFVAFESPE AAETAVEAL NGKEIIDGK PLYVGRAQK KAERQQELK RRFEALKME RLNRYQGVN LYVKNLDDT IDDERLRKE FSPFGTITS AKVMMEDNR SKGFGFVCF SSPEEATKA VTEMNGRIV GSKPLYVAL AQRKEDRKA HLTSQYMQR MANIRMHQM GQFIQPGTS SGYFVPTIP APQRFYGAA QMAQIRTNP RWPAQTPVR PGAQGSANP YGTIPTTYR AAPRPPNQS AALRSNINV PRPITGQQP PNIQGRPLA SQAVVAPAG NRPTTYKYT ANMRNPPQG LGISNAAPV QQAVHIQGQ EPLTATMLA AAPPQEQKQ MLGERLFPL IQRMYPDLA GKITGMLLE IDNSELLHM LEHNESLKA KVEEAVAVL QAHQAKQAA IKKE
- >TC005185 RA
atgaatccg ggtgctcca aactacccc atggcgtct ctgtacgtc ggggatctc cacaccgat atcacggaa gcaatgctt tttgaaaag ttttccact gctggacca gtcctttcg attcgcgtg tgcagagat ttgataacg aggcgttct ctcggttac gcttatgtg aatttccag cagcccgct gatgccgaa cgcgctttg gacaccatg aattttgat ttaatcaag ggccgccca attcgcatt atgtggtcc caacgcgac ccatccctt cgtaagtcg ggagtagga aatgttttc attaagaat ctagatcgt tccattgac aacaaggct atgtacgat acgttctca gcctttgga aacattctc agttgtaaa gtagctcaa gacgaaaac ggcaccagc aaaggctac ggcttcgtc catttcgaa acagaagaa gcggctaac aagtcgatc gaaaaagta aacggcatg ttgctcaac ggaaagaaa gtctacgtt ggccgtttc attccacgt aaagaacgt gaaaaggag cttggcgag aaagctaag ctcttcacc aacgtttac gtcaagaat ttcggcgaa gacttgact gaggagcaa ctgcgcacc atgtttgag aagtacggc aaaatcacc agttacaag atcatgagc aaggacgac ggcaagtcg aaaggattc ggatttgtc gcctttgaa agccctgaa gccgctgag acagccgtt gaagctttg aatggtaag gaaatcatc gatggaaag cctttgtac gttggacgc gcccagaag aaggccgag cgtcagcaa gaattgaaa cgtcgcttc gaagccttg aagatggag cgtttgaac cgctaccaa ggcgtgaac ctctacgtg aaaaacctg gacgacaca attgacgat gaacgttta cgtaaagaa ttctctccg tttggaacc ataacatcg gcgaaagta atgatggag gacaatagg agcaaaggc tttggcttc gtttgtttc tcgtcgccg gaagaggcc acaaaagcc gtgaccgag atgaacggc cggatcgta ggttcaaag cctttatac gtggcctta gcgcagcgc aaggaggac cgtaaagcc cacttgaca tcacaatac atgcagcgt atggcgaac atacgcatg caccaaatg ggtcagttt atccagcct ggaacgtca agtgggtat ttcgttcca acgatccca gccccgcag aggttctat ggggcggcc cagatggcc cagatccgc acgaacccg cgatggccg gctcagacc cccgtgagg cccggagcc cagggcagc gctaacccg tacggtacc attccgacc acgtaccga gccgcgcca aggcccccg aaccagtcc gccgctttg aggagcaat atcaatgtg ccaagacca ataaccggc cagcagcca ccgaatata caaggacga ccattagct agccaagcc gttgtagca cctgcaggt ttaggcatc agcaatgct gctccggtc cagcaagct gtccacatt caaggccag gagcctttg acagccacc atgttggca gctgccccg ccacaagag caaaagcaa atgctgggt gagaggctc ttcccactt attcaacgc atgtatcca gatttggcc ggaaaaatc accggtatg ttgctcgaa attgacaat tcggaactt ttgcatatg ttggaacac aatgaatcc ctcaaagcc aaggtggag gaagccgtt gctgtcttg caggcccat caagccaaa caggctgct attaaaaaa gagtaa
- >TC005185 RB
atgaatccg ggtgctcca aactacccc atggcgtct ctgtacgtc ggggatctc cacaccgat atcacggaa gcaatgctt tttgaaaag ttttccact gctggacca gtcctttcg attcgcgtg tgcagagat ttgataacg aggcgttct ctcggttac gcttatgtg aatttccag cagcccgct gatgccgaa cgcgctttg gacaccatg aattttgat ttaatcaag ggccgccca attcgcatt atgtggtcc caacgcgac ccatccctt cgtaagtcg ggagtagga aatgttttc attaagaat ctagatcgt tccattgac aacaaggct atgtacgat acgttctca gcctttgga aacattctc agttgtaaa gtagctcaa gacgaaaac ggcaccagc aaaggctac ggcttcgtc catttcgaa acagaagaa gcggctaac aagtcgatc gaaaaagta aacggcatg ttgctcaac ggaaagaaa gtctacgtt ggccgtttc attccacgt aaagaacgt gaaaaggag cttggcgag aaagctaag ctcttcacc aacgtttac gtcaagaat ttcggcgaa gacttgact gaggagcaa ctgcgcacc atgtttgag aagtacggc aaaatcacc agttacaag atcatgagc aaggacgac ggcaagtcg aaaggattc ggatttgtc gcctttgaa agccctgaa gccgctgag acagccgtt gaagctttg aatggtaag gaaatcatc gatggaaag cctttgtac gttggacgc gcccagaag aaggccgag cgtcagcaa gaattgaaa cgtcgcttc gaagccttg aagatggag cgtttgaac cgctaccaa ggcgtgaac ctctacgtg aaaaacctg gacgacaca attgacgat gaacgttta cgtaaagaa ttctctccg tttggaacc ataacatcg gcgaaagta atgatggag gacaatagg agcaaaggc tttggcttc gtttgtttc tcgtcgccg gaagaggcc acaaaagcc gtgaccgag atgaacggc cggatcgta ggttcaaag cctttatac gtggcctta gcgcagcgc aaggaggac cgtaaagcc cacttgaca tcacaatac atgcagcgt atggcgaac atacgcatg caccaaatg ggtcagttt atccagcct ggaacgtca agtgggtat ttcgttcca acgatccca gccccgcag aggttctat ggggcggcc cagatggcc cagatccgc acgaacccg cgatggccg gctcagacc cccgtgagg cccggagcc cagggcagc gctaacccg tacggtacc attccgacc acgtaccga gccgcgcca aggcccccg aaccagtcc gccgctttg aggagcaat atcaatgtg ccaagacca ataaccggc cagcagcca ccgaatata caaggacga ccattagct agccaagcc gttgtagca cctgcaggt aatcgccca actacttac aagtataca gcaaatatg cgtaatcct ccacaaggt ttaggcatc agcaatgct gctccggtc cagcaagct gtccacatt caaggccag gagcctttg acagccacc atgttggca gctgccccg ccacaagag caaaagcaa atgctgggt gagaggctc ttcccactt attcaacgc atgtatcca gatttggcc ggaaaaatc accggtatg ttgctcgaa attgacaat tcggaactt ttgcatatg ttggaacac aatgaatcc ctcaaagcc aaggtggag gaagccgtt gctgtcttg caggcccat caagccaaa caggctgct attaaaaaa gagtaa
- >TC005185 RA
tgtgtagtg tgcaagaac gaagatgaa tccgggtgc tccaaacta ccccatggc gtctctgta cgtcgggga tctccacac cgatatcac ggaagcaat gctttttga aaagttttc cactgctgg accagtcct ttcgattcg cgtgtgcag agatttgat aacgaggcg ttctctcgg ttacgctta tgtgaattt ccagcagcc cgctgatgc cgaacgcgc tttggacac catgaattt tgatttaat caagggccg cccaattcg cattatgtg gtcccaacg cgacccatc ccttcgtaa gtcgggagt aggaaatgt tttcattaa gaatctaga tcgttccat tgacaacaa ggctatgta cgatacgtt ctcagcctt tggaaacat tctcagttg taaagtagc tcaagacga aaacggcac cagcaaagg ctacggctt cgtccattt cgaaacaga agaagcggc taacaagtc gatcgaaaa agtaaacgg catgttgct caacggaaa gaaagtcta cgttggccg tttcattcc acgtaaaga acgtgaaaa ggagcttgg cgagaaagc taagctctt caccaacgt ttacgtcaa gaatttcgg cgaagactt gactgagga gcaactgcg caccatgtt tgagaagta cggcaaaat caccagtta caagatcat gagcaagga cgacggcaa gtcgaaagg attcggatt tgtcgcctt tgaaagccc tgaagccgc tgagacagc cgttgaagc tttgaatgg taaggaaat catcgatgg aaagccttt gtacgttgg acgcgccca gaagaaggc cgagcgtca gcaagaatt gaaacgtcg cttcgaagc cttgaagat ggagcgttt gaaccgcta ccaaggcgt gaacctcta cgtgaaaaa cctggacga cacaattga cgatgaacg tttacgtaa agaattctc tccgtttgg aaccataac atcggcgaa agtaatgat ggaggacaa taggagcaa aggctttgg cttcgtttg tttctcgtc gccggaaga ggccacaaa agccgtgac cgagatgaa cggccggat cgtaggttc aaagccttt atacgtggc cttagcgca gcgcaagga ggaccgtaa agcccactt gacatcaca atacatgca gcgtatggc gaacatacg catgcacca aatgggtca gtttatcca gcctggaac gtcaagtgg gtatttcgt tccaacgat cccagcccc gcagaggtt ctatggggc ggcccagat ggcccagat ccgcacgaa cccgcgatg gccggctca gacccccgt gaggcccgg agcccaggg cagcgctaa cccgtacgg taccattcc gaccacgta ccgagccgc gccaaggcc cccgaacca gtccgccgc tttgaggag caatatcaa tgtgccaag accaataac cggccagca gccaccgaa tatacaagg acgaccatt agctagcca agccgttgt agcacctgc aggtttagg catcagcaa tgctgctcc ggtccagca agctgtcca cattcaagg ccaggagcc tttgacagc caccatgtt ggcagctgc cccgccaca agagcaaaa gcaaatgct gggtgagag gctcttccc acttattca acgcatgta tccagattt ggccggaaa aatcaccgg tatgttgct cgaaattga caattcgga acttttgca tatgttgga acacaatga atccctcaa agccaaggt ggaggaagc cgttgctgt cttgcaggc ccatcaagc caaacaggc tgctattaa aaaagagta aacggtcgc ttactcaga caaataaat ataataaaa tgaaaaaaa ttgcaaaca tgtcttacg acaaaaaaa cttatataa aaatcgaaa catcatcat aaatgaaac tattattat gcgttcgtg atttttgct tgtttgcaa ttttcacta tcttagtta aatagttat ttctttggt ttttagtta cttttcgca ttttccaat atcctatta attgatgta tctgcaact tactgaatt tgtaatata aaattgtac atttcatat ttatttttc gttcaaatt aatttctca atcttatgt tactctctt ggtttttgg aattttaaa ataataaat cttaataaa aaca
- >TC005185 RB
tgtgtagtg tgcaagaac gaagatgaa tccgggtgc tccaaacta ccccatggc gtctctgta cgtcgggga tctccacac cgatatcac ggaagcaat gctttttga aaagttttc cactgctgg accagtcct ttcgattcg cgtgtgcag agatttgat aacgaggcg ttctctcgg ttacgctta tgtgaattt ccagcagcc cgctgatgc cgaacgcgc tttggacac catgaattt tgatttaat caagggccg cccaattcg cattatgtg gtcccaacg cgacccatc ccttcgtaa gtcgggagt aggaaatgt tttcattaa gaatctaga tcgttccat tgacaacaa ggctatgta cgatacgtt ctcagcctt tggaaacat tctcagttg taaagtagc tcaagacga aaacggcac cagcaaagg ctacggctt cgtccattt cgaaacaga agaagcggc taacaagtc gatcgaaaa agtaaacgg catgttgct caacggaaa gaaagtcta cgttggccg tttcattcc acgtaaaga acgtgaaaa ggagcttgg cgagaaagc taagctctt caccaacgt ttacgtcaa gaatttcgg cgaagactt gactgagga gcaactgcg caccatgtt tgagaagta cggcaaaat caccagtta caagatcat gagcaagga cgacggcaa gtcgaaagg attcggatt tgtcgcctt tgaaagccc tgaagccgc tgagacagc cgttgaagc tttgaatgg taaggaaat catcgatgg aaagccttt gtacgttgg acgcgccca gaagaaggc cgagcgtca gcaagaatt gaaacgtcg cttcgaagc cttgaagat ggagcgttt gaaccgcta ccaaggcgt gaacctcta cgtgaaaaa cctggacga cacaattga cgatgaacg tttacgtaa agaattctc tccgtttgg aaccataac atcggcgaa agtaatgat ggaggacaa taggagcaa aggctttgg cttcgtttg tttctcgtc gccggaaga ggccacaaa agccgtgac cgagatgaa cggccggat cgtaggttc aaagccttt atacgtggc cttagcgca gcgcaagga ggaccgtaa agcccactt gacatcaca atacatgca gcgtatggc gaacatacg catgcacca aatgggtca gtttatcca gcctggaac gtcaagtgg gtatttcgt tccaacgat cccagcccc gcagaggtt ctatggggc ggcccagat ggcccagat ccgcacgaa cccgcgatg gccggctca gacccccgt gaggcccgg agcccaggg cagcgctaa cccgtacgg taccattcc gaccacgta ccgagccgc gccaaggcc cccgaacca gtccgccgc tttgaggag caatatcaa tgtgccaag accaataac cggccagca gccaccgaa tatacaagg acgaccatt agctagcca agccgttgt agcacctgc aggtaatcg cccaactac ttacaagta tacagcaaa tatgcgtaa tcctccaca aggtttagg catcagcaa tgctgctcc ggtccagca agctgtcca cattcaagg ccaggagcc tttgacagc caccatgtt ggcagctgc cccgccaca agagcaaaa gcaaatgct gggtgagag gctcttccc acttattca acgcatgta tccagattt ggccggaaa aatcaccgg tatgttgct cgaaattga caattcgga acttttgca tatgttgga acacaatga atccctcaa agccaaggt ggaggaagc cgttgctgt cttgcaggc ccatcaagc caaacaggc tgctattaa aaaagagta aacggtcgc ttactcaga caaataaat ataataaaa tgaaaaaaa ttgcaaaca tgtcttacg acaaaaaaa cttatataa aaatcgaaa catcatcat aaatgaaac tattattat gcgttcgtg atttttgct tgtttgcaa ttttcacta tcttagtta aatagttat ttctttggt ttttagtta cttttcgca ttttccaat atcctatta attgatgta tctgcaact tactgaatt tgtaatata aaattgtac atttcatat ttatttttc gttcaaatt aatttctca atcttatgt tactctctt ggtttttgg aattttaaa ataataaat cttaataaa aaca
