Status
Please wait ...
Gene information
General information
TC005185 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4606265..4608714
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005185
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06598

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    remarks: eggs lost,
    Adult morphology
    remarks: eggs lost,
    Analysis of larval stage 1 cuticle
    remarks: eggs lost,
    remarks - cuticles: later than schedule,
     
    8 days later, due to lost eggs in day 9
     

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005185 RA
      MNPGAPNYP MASLYVGDL HTDITEAML FEKFSTAGP 
      VLSIRVCRD LITRRSLGY AYVNFQQPA DAERALDTM 
      NFDLIKGRP IRIMWSQRD PSLRKSGVG NVFIKNLDR 
      SIDNKAMYD TFSAFGNIL SCKVAQDEN GTSKGYGFV 
      HFETEEAAN KSIEKVNGM LLNGKKVYV GRFIPRKER 
      EKELGEKAK LFTNVYVKN FGEDLTEEQ LRTMFEKYG 
      KITSYKIMS KDDGKSKGF GFVAFESPE AAETAVEAL 
      NGKEIIDGK PLYVGRAQK KAERQQELK RRFEALKME 
      RLNRYQGVN LYVKNLDDT IDDERLRKE FSPFGTITS 
      AKVMMEDNR SKGFGFVCF SSPEEATKA VTEMNGRIV 
      GSKPLYVAL AQRKEDRKA HLTSQYMQR MANIRMHQM 
      GQFIQPGTS SGYFVPTIP APQRFYGAA QMAQIRTNP 
      RWPAQTPVR PGAQGSANP YGTIPTTYR AAPRPPNQS 
      AALRSNINV PRPITGQQP PNIQGRPLA SQAVVAPAG 
      LGISNAAPV QQAVHIQGQ EPLTATMLA AAPPQEQKQ 
      MLGERLFPL IQRMYPDLA GKITGMLLE IDNSELLHM 
      LEHNESLKA KVEEAVAVL QAHQAKQAA IKKE
    • >TC005185 RB
      MNPGAPNYP MASLYVGDL HTDITEAML FEKFSTAGP 
      VLSIRVCRD LITRRSLGY AYVNFQQPA DAERALDTM 
      NFDLIKGRP IRIMWSQRD PSLRKSGVG NVFIKNLDR 
      SIDNKAMYD TFSAFGNIL SCKVAQDEN GTSKGYGFV 
      HFETEEAAN KSIEKVNGM LLNGKKVYV GRFIPRKER 
      EKELGEKAK LFTNVYVKN FGEDLTEEQ LRTMFEKYG 
      KITSYKIMS KDDGKSKGF GFVAFESPE AAETAVEAL 
      NGKEIIDGK PLYVGRAQK KAERQQELK RRFEALKME 
      RLNRYQGVN LYVKNLDDT IDDERLRKE FSPFGTITS 
      AKVMMEDNR SKGFGFVCF SSPEEATKA VTEMNGRIV 
      GSKPLYVAL AQRKEDRKA HLTSQYMQR MANIRMHQM 
      GQFIQPGTS SGYFVPTIP APQRFYGAA QMAQIRTNP 
      RWPAQTPVR PGAQGSANP YGTIPTTYR AAPRPPNQS 
      AALRSNINV PRPITGQQP PNIQGRPLA SQAVVAPAG 
      NRPTTYKYT ANMRNPPQG LGISNAAPV QQAVHIQGQ 
      EPLTATMLA AAPPQEQKQ MLGERLFPL IQRMYPDLA 
      GKITGMLLE IDNSELLHM LEHNESLKA KVEEAVAVL 
      QAHQAKQAA IKKE
    CDS sequence
    • >TC005185 RA
      atgaatccg ggtgctcca aactacccc atggcgtct 
      ctgtacgtc ggggatctc cacaccgat atcacggaa 
      gcaatgctt tttgaaaag ttttccact gctggacca 
      gtcctttcg attcgcgtg tgcagagat ttgataacg 
      aggcgttct ctcggttac gcttatgtg aatttccag 
      cagcccgct gatgccgaa cgcgctttg gacaccatg 
      aattttgat ttaatcaag ggccgccca attcgcatt 
      atgtggtcc caacgcgac ccatccctt cgtaagtcg 
      ggagtagga aatgttttc attaagaat ctagatcgt 
      tccattgac aacaaggct atgtacgat acgttctca 
      gcctttgga aacattctc agttgtaaa gtagctcaa 
      gacgaaaac ggcaccagc aaaggctac ggcttcgtc 
      catttcgaa acagaagaa gcggctaac aagtcgatc 
      gaaaaagta aacggcatg ttgctcaac ggaaagaaa 
      gtctacgtt ggccgtttc attccacgt aaagaacgt 
      gaaaaggag cttggcgag aaagctaag ctcttcacc 
      aacgtttac gtcaagaat ttcggcgaa gacttgact 
      gaggagcaa ctgcgcacc atgtttgag aagtacggc 
      aaaatcacc agttacaag atcatgagc aaggacgac 
      ggcaagtcg aaaggattc ggatttgtc gcctttgaa 
      agccctgaa gccgctgag acagccgtt gaagctttg 
      aatggtaag gaaatcatc gatggaaag cctttgtac 
      gttggacgc gcccagaag aaggccgag cgtcagcaa 
      gaattgaaa cgtcgcttc gaagccttg aagatggag 
      cgtttgaac cgctaccaa ggcgtgaac ctctacgtg 
      aaaaacctg gacgacaca attgacgat gaacgttta 
      cgtaaagaa ttctctccg tttggaacc ataacatcg 
      gcgaaagta atgatggag gacaatagg agcaaaggc 
      tttggcttc gtttgtttc tcgtcgccg gaagaggcc 
      acaaaagcc gtgaccgag atgaacggc cggatcgta 
      ggttcaaag cctttatac gtggcctta gcgcagcgc 
      aaggaggac cgtaaagcc cacttgaca tcacaatac 
      atgcagcgt atggcgaac atacgcatg caccaaatg 
      ggtcagttt atccagcct ggaacgtca agtgggtat 
      ttcgttcca acgatccca gccccgcag aggttctat 
      ggggcggcc cagatggcc cagatccgc acgaacccg 
      cgatggccg gctcagacc cccgtgagg cccggagcc 
      cagggcagc gctaacccg tacggtacc attccgacc 
      acgtaccga gccgcgcca aggcccccg aaccagtcc 
      gccgctttg aggagcaat atcaatgtg ccaagacca 
      ataaccggc cagcagcca ccgaatata caaggacga 
      ccattagct agccaagcc gttgtagca cctgcaggt 
      ttaggcatc agcaatgct gctccggtc cagcaagct 
      gtccacatt caaggccag gagcctttg acagccacc 
      atgttggca gctgccccg ccacaagag caaaagcaa 
      atgctgggt gagaggctc ttcccactt attcaacgc 
      atgtatcca gatttggcc ggaaaaatc accggtatg 
      ttgctcgaa attgacaat tcggaactt ttgcatatg 
      ttggaacac aatgaatcc ctcaaagcc aaggtggag 
      gaagccgtt gctgtcttg caggcccat caagccaaa 
      caggctgct attaaaaaa gagtaa
    • >TC005185 RB
      atgaatccg ggtgctcca aactacccc atggcgtct 
      ctgtacgtc ggggatctc cacaccgat atcacggaa 
      gcaatgctt tttgaaaag ttttccact gctggacca 
      gtcctttcg attcgcgtg tgcagagat ttgataacg 
      aggcgttct ctcggttac gcttatgtg aatttccag 
      cagcccgct gatgccgaa cgcgctttg gacaccatg 
      aattttgat ttaatcaag ggccgccca attcgcatt 
      atgtggtcc caacgcgac ccatccctt cgtaagtcg 
      ggagtagga aatgttttc attaagaat ctagatcgt 
      tccattgac aacaaggct atgtacgat acgttctca 
      gcctttgga aacattctc agttgtaaa gtagctcaa 
      gacgaaaac ggcaccagc aaaggctac ggcttcgtc 
      catttcgaa acagaagaa gcggctaac aagtcgatc 
      gaaaaagta aacggcatg ttgctcaac ggaaagaaa 
      gtctacgtt ggccgtttc attccacgt aaagaacgt 
      gaaaaggag cttggcgag aaagctaag ctcttcacc 
      aacgtttac gtcaagaat ttcggcgaa gacttgact 
      gaggagcaa ctgcgcacc atgtttgag aagtacggc 
      aaaatcacc agttacaag atcatgagc aaggacgac 
      ggcaagtcg aaaggattc ggatttgtc gcctttgaa 
      agccctgaa gccgctgag acagccgtt gaagctttg 
      aatggtaag gaaatcatc gatggaaag cctttgtac 
      gttggacgc gcccagaag aaggccgag cgtcagcaa 
      gaattgaaa cgtcgcttc gaagccttg aagatggag 
      cgtttgaac cgctaccaa ggcgtgaac ctctacgtg 
      aaaaacctg gacgacaca attgacgat gaacgttta 
      cgtaaagaa ttctctccg tttggaacc ataacatcg 
      gcgaaagta atgatggag gacaatagg agcaaaggc 
      tttggcttc gtttgtttc tcgtcgccg gaagaggcc 
      acaaaagcc gtgaccgag atgaacggc cggatcgta 
      ggttcaaag cctttatac gtggcctta gcgcagcgc 
      aaggaggac cgtaaagcc cacttgaca tcacaatac 
      atgcagcgt atggcgaac atacgcatg caccaaatg 
      ggtcagttt atccagcct ggaacgtca agtgggtat 
      ttcgttcca acgatccca gccccgcag aggttctat 
      ggggcggcc cagatggcc cagatccgc acgaacccg 
      cgatggccg gctcagacc cccgtgagg cccggagcc 
      cagggcagc gctaacccg tacggtacc attccgacc 
      acgtaccga gccgcgcca aggcccccg aaccagtcc 
      gccgctttg aggagcaat atcaatgtg ccaagacca 
      ataaccggc cagcagcca ccgaatata caaggacga 
      ccattagct agccaagcc gttgtagca cctgcaggt 
      aatcgccca actacttac aagtataca gcaaatatg 
      cgtaatcct ccacaaggt ttaggcatc agcaatgct 
      gctccggtc cagcaagct gtccacatt caaggccag 
      gagcctttg acagccacc atgttggca gctgccccg 
      ccacaagag caaaagcaa atgctgggt gagaggctc 
      ttcccactt attcaacgc atgtatcca gatttggcc 
      ggaaaaatc accggtatg ttgctcgaa attgacaat 
      tcggaactt ttgcatatg ttggaacac aatgaatcc 
      ctcaaagcc aaggtggag gaagccgtt gctgtcttg 
      caggcccat caagccaaa caggctgct attaaaaaa 
      gagtaa
    mRNA sequence
    • >TC005185 RA
      tgtgtagtg tgcaagaac gaagatgaa tccgggtgc 
      tccaaacta ccccatggc gtctctgta cgtcgggga 
      tctccacac cgatatcac ggaagcaat gctttttga 
      aaagttttc cactgctgg accagtcct ttcgattcg 
      cgtgtgcag agatttgat aacgaggcg ttctctcgg 
      ttacgctta tgtgaattt ccagcagcc cgctgatgc 
      cgaacgcgc tttggacac catgaattt tgatttaat 
      caagggccg cccaattcg cattatgtg gtcccaacg 
      cgacccatc ccttcgtaa gtcgggagt aggaaatgt 
      tttcattaa gaatctaga tcgttccat tgacaacaa 
      ggctatgta cgatacgtt ctcagcctt tggaaacat 
      tctcagttg taaagtagc tcaagacga aaacggcac 
      cagcaaagg ctacggctt cgtccattt cgaaacaga 
      agaagcggc taacaagtc gatcgaaaa agtaaacgg 
      catgttgct caacggaaa gaaagtcta cgttggccg 
      tttcattcc acgtaaaga acgtgaaaa ggagcttgg 
      cgagaaagc taagctctt caccaacgt ttacgtcaa 
      gaatttcgg cgaagactt gactgagga gcaactgcg 
      caccatgtt tgagaagta cggcaaaat caccagtta 
      caagatcat gagcaagga cgacggcaa gtcgaaagg 
      attcggatt tgtcgcctt tgaaagccc tgaagccgc 
      tgagacagc cgttgaagc tttgaatgg taaggaaat 
      catcgatgg aaagccttt gtacgttgg acgcgccca 
      gaagaaggc cgagcgtca gcaagaatt gaaacgtcg 
      cttcgaagc cttgaagat ggagcgttt gaaccgcta 
      ccaaggcgt gaacctcta cgtgaaaaa cctggacga 
      cacaattga cgatgaacg tttacgtaa agaattctc 
      tccgtttgg aaccataac atcggcgaa agtaatgat 
      ggaggacaa taggagcaa aggctttgg cttcgtttg 
      tttctcgtc gccggaaga ggccacaaa agccgtgac 
      cgagatgaa cggccggat cgtaggttc aaagccttt 
      atacgtggc cttagcgca gcgcaagga ggaccgtaa 
      agcccactt gacatcaca atacatgca gcgtatggc 
      gaacatacg catgcacca aatgggtca gtttatcca 
      gcctggaac gtcaagtgg gtatttcgt tccaacgat 
      cccagcccc gcagaggtt ctatggggc ggcccagat 
      ggcccagat ccgcacgaa cccgcgatg gccggctca 
      gacccccgt gaggcccgg agcccaggg cagcgctaa 
      cccgtacgg taccattcc gaccacgta ccgagccgc 
      gccaaggcc cccgaacca gtccgccgc tttgaggag 
      caatatcaa tgtgccaag accaataac cggccagca 
      gccaccgaa tatacaagg acgaccatt agctagcca 
      agccgttgt agcacctgc aggtttagg catcagcaa 
      tgctgctcc ggtccagca agctgtcca cattcaagg 
      ccaggagcc tttgacagc caccatgtt ggcagctgc 
      cccgccaca agagcaaaa gcaaatgct gggtgagag 
      gctcttccc acttattca acgcatgta tccagattt 
      ggccggaaa aatcaccgg tatgttgct cgaaattga 
      caattcgga acttttgca tatgttgga acacaatga 
      atccctcaa agccaaggt ggaggaagc cgttgctgt 
      cttgcaggc ccatcaagc caaacaggc tgctattaa 
      aaaagagta aacggtcgc ttactcaga caaataaat 
      ataataaaa tgaaaaaaa ttgcaaaca tgtcttacg 
      acaaaaaaa cttatataa aaatcgaaa catcatcat 
      aaatgaaac tattattat gcgttcgtg atttttgct 
      tgtttgcaa ttttcacta tcttagtta aatagttat 
      ttctttggt ttttagtta cttttcgca ttttccaat 
      atcctatta attgatgta tctgcaact tactgaatt 
      tgtaatata aaattgtac atttcatat ttatttttc 
      gttcaaatt aatttctca atcttatgt tactctctt 
      ggtttttgg aattttaaa ataataaat cttaataaa 
      aaca
    • >TC005185 RB
      tgtgtagtg tgcaagaac gaagatgaa tccgggtgc 
      tccaaacta ccccatggc gtctctgta cgtcgggga 
      tctccacac cgatatcac ggaagcaat gctttttga 
      aaagttttc cactgctgg accagtcct ttcgattcg 
      cgtgtgcag agatttgat aacgaggcg ttctctcgg 
      ttacgctta tgtgaattt ccagcagcc cgctgatgc 
      cgaacgcgc tttggacac catgaattt tgatttaat 
      caagggccg cccaattcg cattatgtg gtcccaacg 
      cgacccatc ccttcgtaa gtcgggagt aggaaatgt 
      tttcattaa gaatctaga tcgttccat tgacaacaa 
      ggctatgta cgatacgtt ctcagcctt tggaaacat 
      tctcagttg taaagtagc tcaagacga aaacggcac 
      cagcaaagg ctacggctt cgtccattt cgaaacaga 
      agaagcggc taacaagtc gatcgaaaa agtaaacgg 
      catgttgct caacggaaa gaaagtcta cgttggccg 
      tttcattcc acgtaaaga acgtgaaaa ggagcttgg 
      cgagaaagc taagctctt caccaacgt ttacgtcaa 
      gaatttcgg cgaagactt gactgagga gcaactgcg 
      caccatgtt tgagaagta cggcaaaat caccagtta 
      caagatcat gagcaagga cgacggcaa gtcgaaagg 
      attcggatt tgtcgcctt tgaaagccc tgaagccgc 
      tgagacagc cgttgaagc tttgaatgg taaggaaat 
      catcgatgg aaagccttt gtacgttgg acgcgccca 
      gaagaaggc cgagcgtca gcaagaatt gaaacgtcg 
      cttcgaagc cttgaagat ggagcgttt gaaccgcta 
      ccaaggcgt gaacctcta cgtgaaaaa cctggacga 
      cacaattga cgatgaacg tttacgtaa agaattctc 
      tccgtttgg aaccataac atcggcgaa agtaatgat 
      ggaggacaa taggagcaa aggctttgg cttcgtttg 
      tttctcgtc gccggaaga ggccacaaa agccgtgac 
      cgagatgaa cggccggat cgtaggttc aaagccttt 
      atacgtggc cttagcgca gcgcaagga ggaccgtaa 
      agcccactt gacatcaca atacatgca gcgtatggc 
      gaacatacg catgcacca aatgggtca gtttatcca 
      gcctggaac gtcaagtgg gtatttcgt tccaacgat 
      cccagcccc gcagaggtt ctatggggc ggcccagat 
      ggcccagat ccgcacgaa cccgcgatg gccggctca 
      gacccccgt gaggcccgg agcccaggg cagcgctaa 
      cccgtacgg taccattcc gaccacgta ccgagccgc 
      gccaaggcc cccgaacca gtccgccgc tttgaggag 
      caatatcaa tgtgccaag accaataac cggccagca 
      gccaccgaa tatacaagg acgaccatt agctagcca 
      agccgttgt agcacctgc aggtaatcg cccaactac 
      ttacaagta tacagcaaa tatgcgtaa tcctccaca 
      aggtttagg catcagcaa tgctgctcc ggtccagca 
      agctgtcca cattcaagg ccaggagcc tttgacagc 
      caccatgtt ggcagctgc cccgccaca agagcaaaa 
      gcaaatgct gggtgagag gctcttccc acttattca 
      acgcatgta tccagattt ggccggaaa aatcaccgg 
      tatgttgct cgaaattga caattcgga acttttgca 
      tatgttgga acacaatga atccctcaa agccaaggt 
      ggaggaagc cgttgctgt cttgcaggc ccatcaagc 
      caaacaggc tgctattaa aaaagagta aacggtcgc 
      ttactcaga caaataaat ataataaaa tgaaaaaaa 
      ttgcaaaca tgtcttacg acaaaaaaa cttatataa 
      aaatcgaaa catcatcat aaatgaaac tattattat 
      gcgttcgtg atttttgct tgtttgcaa ttttcacta 
      tcttagtta aatagttat ttctttggt ttttagtta 
      cttttcgca ttttccaat atcctatta attgatgta 
      tctgcaact tactgaatt tgtaatata aaattgtac 
      atttcatat ttatttttc gttcaaatt aatttctca 
      atcttatgt tactctctt ggtttttgg aattttaaa 
      ataataaat cttaataaa aaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY