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Gene information
General information
TC031524 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4638656..4642181
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031524
      iBeetle screen iB_06596

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      remarks: eggs lost,
      Adult morphology
      remarks: eggs lost,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      remarks: eggs lost,
      remarks - cuticles: later than schedule,
       
      8 days later, due to lost eggs in day 9
       

      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031524 RA
        MAYKPEGLF EHILIHYRW VFVCLFLLP ASFFFDIWL 
        YVRNWIVFK LSSAPKQHH SKVKHVQTQ VRNWIKNGR 
        KQFMCTARP GWQTVSFRR PKYKKTMYN VEVNLVDVL 
        DINLEKKTV RVEPLVTMG QLTATLNPL GWTIPVVPE 
        LDDLTVGGL VMGTGIESS SHKYGLFQH ICVSYELVL 
        CDGSVVTCS TQENPNLYY SVPWSYGTL GILTAVEIQ 
        LIPAKKYVK LTYKPLKTL DQVTQETES ASKDLNNEF 
        VETLVYSKD KAVLMTGIQ TDQVDENKV NAIGKWYKP 
        WFFKHVEGM LETNKTVVE YIPLRDYYH RHTRSIFWE 
        LQDIIPFGN NLLFRFFLG WIVPPKVSL LKLTQTEAV 
        RKLYENNHV IQDMLVPIE TLKASIEKF HEVLEIYPM 
        WICPFKLPP NPGMVHPPL KNYDTDPVY VDIGVYGVP 
        KAKNFTPLG STREIEKYV SNGNGFQML YADTYMSRE 
        EFRRMFDHR LYDSLRASL GCESAFAEV YDKVSRAAR 
        E
      CDS sequence
      • >TC031524 RA
        atggcttac aagccggag ggactcttc gagcacatc 
        ctgattcac tacagatgg gttttcgtt tgtcttttc 
        ctcttgccc gcctctttc tttttcgat atttggctg 
        tacgtccgt aactggatc gttttcaaa ttgagcagt 
        gcccccaaa caacatcat tccaaagtg aaacatgta 
        caaacccag gtaagaaat tggattaag aatggaaga 
        aaacaattt atgtgcaca gccaggcct ggatggcag 
        acagtctcc ttcaggagg cccaaatat aagaagact 
        atgtacaat gttgaagtc aatctagtt gacgtgctt 
        gatattaat ttagagaag aagactgtt cgggttgaa 
        cctttagtc accatgggt cagttaacc gcgactttg 
        aaccctcta ggctggact attccggtg gtaccggag 
        ctcgatgat ttaacagtc ggaggttta gttatgggg 
        actggaata gagtcgtct tcgcacaaa tacggcctc 
        ttccaacac atttgcgtt tcttacgaa ttagttctt 
        tgtgatggt tctgtggta acttgttca actcaagaa 
        aacccaaat ttatactat tcagtacct tggtcgtac 
        ggcactttg ggtatattg acagccgtt gaaattcag 
        ttaatccca gccaaaaaa tacgtcaaa ctgacttac 
        aaaccctta aaaaccctg gatcaagta actcaagag 
        actgaatca gcaagtaaa gacctgaac aacgaattt 
        gtcgaaact ttggtttat agtaaagat aaggcggtg 
        ctcatgacc ggcattcaa accgaccaa gttgacgaa 
        aataaagtg aatgcgatt ggaaaatgg tacaagcct 
        tggtttttc aaacatgtt gaaggcatg ttggaaact 
        aataaaact gtggttgaa tacatcccc ttgcgtgac 
        tattaccac cgacacacg agatctata ttttgggaa 
        cttcaggac attattccc ttcggaaac aacctcctg 
        tttaggttt ttcttgggt tggattgtg cccccaaaa 
        gtctccctt ttaaaactg acccaaact gaagctgtg 
        cgaaaactg tacgagaac aaccacgtc attcaggac 
        atgttagta ccgattgaa accctcaaa gcgagcatc 
        gagaaattt cacgaggtt ctcgagata taccccatg 
        tggatttgc ccttttaag ttaccccca aacccggga 
        atggtgcac cctccccta aaaaactac gatacggac 
        cccgtttac gtcgatatc ggtgtgtac ggggtaccc 
        aaagctaaa aatttcaca ccgcttgga tcgacgaga 
        gagatcgaa aaatacgtc agtaatggc aatggtttc 
        cagatgctg tatgccgac acatacatg tcccgtgag 
        gaatttcgc agaatgttc gaccaccgg ctgtacgac 
        tcgctgagg gcttctctg ggctgcgaa tcggccttc 
        gccgaggtg tacgacaag gtgagcagg gccgctcgg 
        gagtag
      mRNA sequence
      • >TC031524 RA
        tttatcagt tgggtctgt gaacatggc ttacaagcc 
        ggagggact cttcgagca catcctgat tcactacag 
        atgggtttt cgtttgtct tttcctctt gcccgcctc 
        tttcttttt cgatatttg gctgtacgt ccgtaactg 
        gatcgtttt caaattgag cagtgcccc caaacaaca 
        tcattccaa agtgaaaca tgtacaaac ccaggtaag 
        aaattggat taagaatgg aagaaaaca atttatgtg 
        cacagccag gcctggatg gcagacagt ctccttcag 
        gaggcccaa atataagaa gactatgta caatgttga 
        agtcaatct agttgacgt gcttgatat taatttaga 
        gaagaagac tgttcgggt tgaaccttt agtcaccat 
        gggtcagtt aaccgcgac tttgaaccc tctaggctg 
        gactattcc ggtggtacc ggagctcga tgatttaac 
        agtcggagg tttagttat ggggactgg aatagagtc 
        gtcttcgca caaatacgg cctcttcca acacatttg 
        cgtttctta cgaattagt tctttgtga tggttctgt 
        ggtaacttg ttcaactca agaaaaccc aaatttata 
        ctattcagt accttggtc gtacggcac tttgggtat 
        attgacagc cgttgaaat tcagttaat cccagccaa 
        aaaatacgt caaactgac ttacaaacc cttaaaaac 
        cctggatca agtaactca agagactga atcagcaag 
        taaagacct gaacaacga atttgtcga aactttggt 
        ttatagtaa agataaggc ggtgctcat gaccggcat 
        tcaaaccga ccaagttga cgaaaataa agtgaatgc 
        gattggaaa atggtacaa gccttggtt tttcaaaca 
        tgttgaagg catgttgga aactaataa aactgtggt 
        tgaatacat ccccttgcg tgactatta ccaccgaca 
        cacgagatc tatattttg ggaacttca ggacattat 
        tcccttcgg aaacaacct cctgtttag gtttttctt 
        gggttggat tgtgccccc aaaagtctc ccttttaaa 
        actgaccca aactgaagc tgtgcgaaa actgtacga 
        gaacaacca cgtcattca ggacatgtt agtaccgat 
        tgaaaccct caaagcgag catcgagaa atttcacga 
        ggttctcga gatataccc catgtggat ttgcccttt 
        taagttacc cccaaaccc gggaatggt gcaccctcc 
        cctaaaaaa ctacgatac ggaccccgt ttacgtcga 
        tatcggtgt gtacggggt acccaaagc taaaaattt 
        cacaccgct tggatcgac gagagagat cgaaaaata 
        cgtcagtaa tggcaatgg tttccagat gctgtatgc 
        cgacacata catgtcccg tgaggaatt tcgcagaat 
        gttcgacca ccggctgta cgactcgct gagggcttc 
        tctgggctg cgaatcggc cttcgccga ggtgtacga 
        caaggtgag cagggccgc tcgggagta ggaactgaa 
        gaaaccact ggagctgca ctatttgct ttcggaagt 
        cacggcctg tggccatca tcattccag aaataaaga 
        atgttgaaa aga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY