| General information | |||
| TC005170 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 4642273..4648190 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005170 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06595 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03914 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
| 4 |
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC005170 RA
MPVDKLHSE YRSTYRWHE YTGPRQEVV RRPPQPNAA PNAGDTQHS SKEDEVHNE LPKLEPAMP RRKKYPDLA YRHHEFLIS DGEITANGV CNTEPRARS EERGAHWRP SRRSKSEGP RGGGGHGDG MRERERRRE SDPDLENMG RETGLLRKA ISKISTEYR LQFAWPQGH TSRRDAVDS SAPRKSQSM GALKPATNA MVHKKRIDL ENKDASELE PLVDERNTR EDKTHIFKD EFNSEYKKN FRPFSQYEY SEGRFTKRG APVDDTDNS AGQNSLPPQ CEKNDSWYR EVVELRKKA GEYKHRGWG SELAPDRLS DIYNKQVEL WDQVSRRSS LSALSLASM THKSYTKEE KEVDNNNKT SPSKAARNA ENSARIVRD MIRHHLERT TGGSEFDGL ILSPTREKL EPTIPRKDD DSRSSQKNS PKKHSPMKS SSLKRHNQK APKSGPKNL RSQSVGPVT DTTEKRSPK RQSRSATVK ERKSSANQA SIKRPRPSS LNTTVSSRS KHSSVPSKT EDDRLAKAN SKSNTKQKL GTKQLEKGN SGADPPKTE ANEEVKNAP VEAPEIIDY EPVVKSPPE PTRVKSPEQ ILMRSPDPV NWTVPLDTG KTFTVTQNV REGDMSSRP HSEVKAWTP PDVPLPIAQ SAPPTLDQN KEQGMSYGF SSESMNSGQ GRVIAAQLR DLQPTVEHL RPRSIADAS FNRVVARKC RNGHGFVTP WCMFARTEQ GTYTTASRH LLNLI
- >TC005170 RB
MPVDKLHSE YRSTYRWHE YTGPRQEVV RRPPQPNAA PNAVPKLEP AMPRRKKYP DLAYRHHEF LISDGEITA NGVCNTEPR ARSEERGAH WRPSRRSKS EGPRGGGGH GDGMRERER RRESDPDLE NMGRETGLL RKAISKIST EYRLQFAWP QGHTSRRDA VDSSAPRKS QSMGALKPA TNAMVHKKR IDLENKDAS ELEPLVDER NTREDKTHI FKDEFNSEY KKNFRPFSQ YEYSEGRFT KRGAPVDDT DNSAGQNSL PPQCEKNDS WYREVVELR KKAGEYKHR GWGSELAPD RLSDIYNKQ VELWDQVSR RSSLSALSL ASMTHKSYT KEEKEVDNN NKTSPSKAA RNAENSARI VRDMIRHHL ERTTGGSEF DGLILSPTR EKLEPTIPR KDDDSRSSQ KNSPKKHSP MKSSSLKRH NQKAPKSGP KNLRSQSVG PVTDTTEKR SPKRQSRSA TVKERKSSA NQASIKRPR PSSLNTTVS SRSKHSSVP SKTEDDRLA KANSKSNTK QKLGTKQLE KGNSGADPP KTEANEEVK NAPVEAPEI IDYEPVVKS PPEPTRVKS PEQILMRSP DPVNWTVPL DTGKTFTVT QNVREGDMS SRPHSEVKA WTPPDVPLP IAQSAPPTL DQNKEQGMS YGFSSESMN SGQGRVIAA QLRDLQPTV EHLRPRSIA DASFNRVVA RKCRNGHGF VTPWCMFAR TEQGTYTTA SRHLLNLI
- >TC005170 RA
atgcctgtc gataagctg cactccgag taccggagt acttaccga tggcacgag tacacgggg cccaggcag gaggtcgtg cggaggcca ccgcagccc aacgccgct ccgaacgcc ggcgacact cagcactcc tccaaggag gatgaggtg cataatgag ctgcccaag ctggaaccg gcgatgcct aggaggaag aagtatccc gacttggcc taccgccac cacgagttt ctgatcagt gatggcgaa atcaccgcg aatggggtg tgcaacacg gaacctagg gctcggtcg gaggagcgt ggggcgcac tggaggcct tcccggcgg agcaagtcg gagggaccg cgtggcggc ggcggccac ggggacggc atgcgggag cgggagcgg cgtagggaa tcggacccg gatttggag aacatggga cgcgaaact gggcttctc cggaaggcc atttcaaaa attagcact gaatacaga ctgcaattc gcttggcct cagggccat acttcacgt agagatgcc gtcgattcc tcggcacct cgcaaatcc caatcgatg ggagcgcta aaaccggcc accaatgca atggtgcac aaaaaacgc atcgatttg gaaaataag gatgccagt gaactcgaa ccattagtc gatgaaaga aacacacgt gaagacaag actcacata ttcaaggat gaattcaat tccgaatac aagaagaac ttcagacct ttctcccag tatgaatac tcggaagga cgcttcacc aaacgggga gctcccgtc gatgacacc gataactcg gccggacaa aactcgcta ccgcctcaa tgcgagaag aacgactct tggtacagg gaagtcgtc gaattgcga aaaaaggca ggagaatat aagcaccgc ggttggggt agtgaactt gctccagat aggctttct gatatatac aataagcaa gttgaactt tgggatcaa gtttcacgg agaagttct ctatcagcc ttatctctc gcttccatg acacacaag tcttataca aaagaagag aaggaagta gataataac aacaagact tctccatcg aaagcggct cgcaatgct gaaaactca gctcgaatt gtacgagat atgatcaga catcattta gaacgaaca actggtggt tcagaattt gacggtttg attctgtcg ccgactaga gagaaactc gaaccgact ataccacgc aaagatgat gattcgaga agttcgcaa aagaacagc cccaagaaa cactcgccg atgaaatca tcatctttg aagcgacat aaccagaaa gcccccaaa agcggtcct aagaaccta cgttcccag tctgttggt cctgtgact gatacgact gaaaaacgg tcaccaaag cgccagtca cggtctgcc accgtcaaa gagcgaaaa tcttcggca aatcaagct agcatcaaa agacctcga ccttcatcc ttgaacact actgtctca tcccgatct aaacatagc tcggtaccc tctaaaacc gaagacgac agattggcc aaagctaat tcaaaatct aacactaag caaaaacta ggcactaaa cagttagaa aaaggaaat tctggagcc gacccaccc aagaccgaa gctaacgaa gaagtgaag aacgcccca gtcgaagca cccgaaata atcgattac gagccggtg gtaaagtcg ccgccggaa ccgactagg gtgaaatct cccgaacaa attctgatg cgttccccg gatcctgtc aactggact gtgccactc gacaccgga aaaactttt actgtcaca caaaacgtc agggagggg gatatgagt agtcgaccc catagcgaa gtgaaagcc tggactccc ccagacgtg ccccttccc atcgcccag tctgctcct ccgactctt gatcaaaat aaggaacaa ggcatgtcg tatggattt agcagtgaa agcatgaac tcgggccag ggcagagtc atcgctgca cagctgaga gacctccaa ccaacggta gagcatcta aggccacgt tccatcgca gatgcatct ttcaacagg gtagtagcc agaaagtgt cggaatggc cacggtttt gtaactccg tggtgtatg ttcgcaagg actgaacaa gggacatac accacggct tcgcgccat ttgctgaac cttatttga
- >TC005170 RB
atgcctgtc gataagctg cactccgag taccggagt acttaccga tggcacgag tacacgggg cccaggcag gaggtcgtg cggaggcca ccgcagccc aacgccgct ccgaacgcc gtgcccaag ctggaaccg gcgatgcct aggaggaag aagtatccc gacttggcc taccgccac cacgagttt ctgatcagt gatggcgaa atcaccgcg aatggggtg tgcaacacg gaacctagg gctcggtcg gaggagcgt ggggcgcac tggaggcct tcccggcgg agcaagtcg gagggaccg cgtggcggc ggcggccac ggggacggc atgcgggag cgggagcgg cgtagggaa tcggacccg gatttggag aacatggga cgcgaaact gggcttctc cggaaggcc atttcaaaa attagcact gaatacaga ctgcaattc gcttggcct cagggccat acttcacgt agagatgcc gtcgattcc tcggcacct cgcaaatcc caatcgatg ggagcgcta aaaccggcc accaatgca atggtgcac aaaaaacgc atcgatttg gaaaataag gatgccagt gaactcgaa ccattagtc gatgaaaga aacacacgt gaagacaag actcacata ttcaaggat gaattcaat tccgaatac aagaagaac ttcagacct ttctcccag tatgaatac tcggaagga cgcttcacc aaacgggga gctcccgtc gatgacacc gataactcg gccggacaa aactcgcta ccgcctcaa tgcgagaag aacgactct tggtacagg gaagtcgtc gaattgcga aaaaaggca ggagaatat aagcaccgc ggttggggt agtgaactt gctccagat aggctttct gatatatac aataagcaa gttgaactt tgggatcaa gtttcacgg agaagttct ctatcagcc ttatctctc gcttccatg acacacaag tcttataca aaagaagag aaggaagta gataataac aacaagact tctccatcg aaagcggct cgcaatgct gaaaactca gctcgaatt gtacgagat atgatcaga catcattta gaacgaaca actggtggt tcagaattt gacggtttg attctgtcg ccgactaga gagaaactc gaaccgact ataccacgc aaagatgat gattcgaga agttcgcaa aagaacagc cccaagaaa cactcgccg atgaaatca tcatctttg aagcgacat aaccagaaa gcccccaaa agcggtcct aagaaccta cgttcccag tctgttggt cctgtgact gatacgact gaaaaacgg tcaccaaag cgccagtca cggtctgcc accgtcaaa gagcgaaaa tcttcggca aatcaagct agcatcaaa agacctcga ccttcatcc ttgaacact actgtctca tcccgatct aaacatagc tcggtaccc tctaaaacc gaagacgac agattggcc aaagctaat tcaaaatct aacactaag caaaaacta ggcactaaa cagttagaa aaaggaaat tctggagcc gacccaccc aagaccgaa gctaacgaa gaagtgaag aacgcccca gtcgaagca cccgaaata atcgattac gagccggtg gtaaagtcg ccgccggaa ccgactagg gtgaaatct cccgaacaa attctgatg cgttccccg gatcctgtc aactggact gtgccactc gacaccgga aaaactttt actgtcaca caaaacgtc agggagggg gatatgagt agtcgaccc catagcgaa gtgaaagcc tggactccc ccagacgtg ccccttccc atcgcccag tctgctcct ccgactctt gatcaaaat aaggaacaa ggcatgtcg tatggattt agcagtgaa agcatgaac tcgggccag ggcagagtc atcgctgca cagctgaga gacctccaa ccaacggta gagcatcta aggccacgt tccatcgca gatgcatct ttcaacagg gtagtagcc agaaagtgt cggaatggc cacggtttt gtaactccg tggtgtatg ttcgcaagg actgaacaa gggacatac accacggct tcgcgccat ttgctgaac cttatttga
- >TC005170 RA
aaacgctcg agatttgac gaattttgt tgccacacg acggcgtca gcccgcatt gctgtggct taattaatt atgcaaggg agctttcgt gttcacttt ttattcaaa agccccaaa tgtcgagta tgacgtcgc tggaagaaa atttttggt tcgcattaa tcaaccggc gactgcact caacagatt cagaagtgc aacgctatg ttgcattat gttgtgcgg atgtgaaaa agcaatgca atttacggt tttctgtgt taaatccaa aaaaccgta actggcatg aacccgttt aggaaaagt tgcacttgc attattcat aatttagga atccatttt ctagaatat gggagcagt taacattgt tttattaca tacagataa ggttttata atcgggatc gtctgcctg gaaaacaaa cgatatttt attcatgat ctgtattct aatgcgtcg gattaacga aacgttcat gtttttatt ttgtgttgt attaggagg gcgggtgct aaaaccatg gaggttttt catgatgtg gtttgaagc aaatttgga tcgggtggt agtaaagcg ttcagtagt ctatgcttc attataatt agtaaattt ggttaaaat ggtatagcc gactatgca atcgatagt cgctttatt ggtcgtatt gtgtgacct ttgaaagtc aggtttgta gttaaaaca gccttaaat gagcaaatg tcaatggga tagattagg tggcattat ttaatttcc cttgtaatg gtttttcct ttgtttaag cgagtggag tcgagtaaa attggattt aaaagttgt gtgtctttg aaaaagttt aatttgttg tatgatgtt agaaattta atttgtgtg ttacataaa tgatattgc ctgtccggg gtctggaat gaataaaat gtctcggga agaattttg cagggttgg gaaaaatgt gaggaaagg gggtggtag gtcagagat gatgtctac tgaccgatc gtccttgat tcccccatt ttggggtga atgacctcg cacattgaa tttccctgt gagcgtgtt gggggtgag ggctgaata tcgttttgc aggtgattt cgctgctat tgaacgaat tggaattgg agtaatagg aatcgagtc cggtgataa taattcggg gtatttgtt gtggatggc gctctaagt gggtaaaaa acagtagcg caactggcg caacgatag aattgcaag gagcaggcg cccgtctat tgattctta ggttgagta gagccgtcg tcgacccga ccacggatt gccaataag gtggggctc ctgtcatgc tgcacgagc tctcgtgtc cgtcgagtc tggtggctc attaagttc tcgcgacct acgccgtct cctctcacg ttttctggc ccccggagt ttccggtgc ggtgtatgg cttgtcggc ctcagagtt acttggaga gaatttaag tgcccattc gacctgatg attggctca ttctggaac ttgtgcaga gcttgcccg tcgatgcct gtcgataag ctgcactcc gagtaccgg agtacttac cgatggcac gagtacacg gggcccagg caggaggtc gtgcggagg ccaccgcag cccaacgcc gctccgaac gccggcgac actcagcac tcctccaag gaggatgag gtgcataat gagctgccc aagctggaa ccggcgatg cctaggagg aagaagtat cccgacttg gcctaccgc caccacgag tttctgatc agtgatggc gaaatcacc gcgaatggg gtgtgcaac acggaacct agggctcgg tcggaggag cgtggggcg cactggagg ccttcccgg cggagcaag tcggaggga ccgcgtggc ggcggcggc cacggggac ggcatgcgg gagcgggag cggcgtagg gaatcggac ccggatttg gagaacatg ggacgcgaa actgggctt ctccggaag gccatttca aaaattagc actgaatac agactgcaa ttcgcttgg cctcagggc catacttca cgtagagat gccgtcgat tcctcggca cctcgcaaa tcccaatcg atgggagcg ctaaaaccg gccaccaat gcaatggtg cacaaaaaa cgcatcgat ttggaaaat aaggatgcc agtgaactc gaaccatta gtcgatgaa agaaacaca cgtgaagac aagactcac atattcaag gatgaattc aattccgaa tacaagaag aacttcaga cctttctcc cagtatgaa tactcggaa ggacgcttc accaaacgg ggagctccc gtcgatgac accgataac tcggccgga caaaactcg ctaccgcct caatgcgag aagaacgac tcttggtac agggaagtc gtcgaattg cgaaaaaag gcaggagaa tataagcac cgcggttgg ggtagtgaa cttgctcca gataggctt tctgatata tacaataag caagttgaa ctttgggat caagtttca cggagaagt tctctatca gccttatct ctcgcttcc atgacacac aagtcttat acaaaagaa gagaaggaa gtagataat aacaacaag acttctcca tcgaaagcg gctcgcaat gctgaaaac tcagctcga attgtacga gatatgatc agacatcat ttagaacga acaactggt ggttcagaa tttgacggt ttgattctg tcgccgact agagagaaa ctcgaaccg actatacca cgcaaagat gatgattcg agaagttcg caaaagaac agccccaag aaacactcg ccgatgaaa tcatcatct ttgaagcga cataaccag aaagccccc aaaagcggt cctaagaac ctacgttcc cagtctgtt ggtcctgtg actgatacg actgaaaaa cggtcacca aagcgccag tcacggtct gccaccgtc aaagagcga aaatcttcg gcaaatcaa gctagcatc aaaagacct cgaccttca tccttgaac actactgtc tcatcccga tctaaacat agctcggta ccctctaaa accgaagac gacagattg gccaaagct aattcaaaa tctaacact aagcaaaaa ctaggcact aaacagtta gaaaaagga aattctgga gccgaccca cccaagacc gaagctaac gaagaagtg aagaacgcc ccagtcgaa gcacccgaa ataatcgat tacgagccg gtggtaaag tcgccgccg gaaccgact agggtgaaa tctcccgaa caaattctg atgcgttcc ccggatcct gtcaactgg actgtgcca ctcgacacc ggaaaaact tttactgtc acacaaaac gtcagggag ggggatatg agtagtcga ccccatagc gaagtgaaa gcctggact cccccagac gtgcccctt cccatcgcc cagtctgct cctccgact cttgatcaa aataaggaa caaggcatg tcgtatgga tttagcagt gaaagcatg aactcgggc cagggcaga gtcatcgct gcacagctg agagacctc caaccaacg gtagagcat ctaaggcca cgttccatc gcagatgca tctttcaac agggtagta gccagaaag tgtcggaat ggccacggt tttgtaact ccgtggtgt atgttcgca aggactgaa caagggaca tacaccacg gcttcgcgc catttgctg aaccttatt tgagccgtt tgcccagct ttacctttg taatcaact gcttatgtt ccatgccat ccgtttaca aatccgtca aggtgccac atttcgttc gaaattatg gacgtcccc tttatacaa aaatttgtg atctctaat cagtgcact cgtgttttc tgccacaag gaagtcaga cgaaactat ctatggttg acatatatt catatattt tgtgtagat gcgtttgct tgccaactc tctggttaa tttagtgac atcgaacgc acttatgtt atttattat taatcatac agtaaagag ttatataaa agttt
- >TC005170 RB
aaacgctcg agatttgac gaattttgt tgccacacg acggcgtca gcccgcatt gctgtggct taattaatt atgcaaggg agctttcgt gttcacttt ttattcaaa agccccaaa tgtcgagta tgacgtcgc tggaagaaa atttttggt tcgcattaa tcaaccggc gactgcact caacagatt cagaagtgc aacgctatg ttgcattat gttgtgcgg atgtgaaaa agcaatgca atttacggt tttctgtgt taaatccaa aaaaccgta actggcatg aacccgttt aggaaaagt tgcacttgc attattcat aatttagga atccatttt ctagaatat gggagcagt taacattgt tttattaca tacagataa ggttttata atcgggatc gtctgcctg gaaaacaaa cgatatttt attcatgat ctgtattct aatgcgtcg gattaacga aacgttcat gtttttatt ttgtgttgt attaggagg gcgggtgct aaaaccatg gaggttttt catgatgtg gtttgaagc aaatttgga tcgggtggt agtaaagcg ttcagtagt ctatgcttc attataatt agtaaattt ggttaaaat ggtatagcc gactatgca atcgatagt cgctttatt ggtcgtatt gtgtgacct ttgaaagtc aggtttgta gttaaaaca gccttaaat gagcaaatg tcaatggga tagattagg tggcattat ttaatttcc cttgtaatg gtttttcct ttgtttaag cgagtggag tcgagtaaa attggattt aaaagttgt gtgtctttg aaaaagttt aatttgttg tatgatgtt agaaattta atttgtgtg ttacataaa tgatattgc ctgtccggg gtctggaat gaataaaat gtctcggga agaattttg cagggttgg gaaaaatgt gaggaaagg gggtggtag gtcagagat gatgtctac tgaccgatc gtccttgat tcccccatt ttggggtga atgacctcg cacattgaa tttccctgt gagcgtgtt gggggtgag ggctgaata tcgttttgc aggtgattt cgctgctat tgaacgaat tggaattgg agtaatagg aatcgagtc cggtgataa taattcggg gtatttgtt gtggatggc gctctaagt gggtaaaaa acagtagcg caactggcg caacgatag aattgcaag gagcaggcg cccgtctat tgattctta ggttgagta gagccgtcg tcgacccga ccacggatt gccaataag gtggggctc ctgtcatgc tgcacgagc tctcgtgtc cgtcgagtc tggtggctc attaagttc tcgcgacct acgccgtct cctctcacg ttttctggc ccccggagt ttccggtgc ggtgtatgg cttgtcggc ctcagagtt acttggaga gaatttaag tgcccattc gacctgatg attggctca ttctggaac ttgtgcaga gcttgcccg tcgatgcct gtcgataag ctgcactcc gagtaccgg agtacttac cgatggcac gagtacacg gggcccagg caggaggtc gtgcggagg ccaccgcag cccaacgcc gctccgaac gccgtgccc aagctggaa ccggcgatg cctaggagg aagaagtat cccgacttg gcctaccgc caccacgag tttctgatc agtgatggc gaaatcacc gcgaatggg gtgtgcaac acggaacct agggctcgg tcggaggag cgtggggcg cactggagg ccttcccgg cggagcaag tcggaggga ccgcgtggc ggcggcggc cacggggac ggcatgcgg gagcgggag cggcgtagg gaatcggac ccggatttg gagaacatg ggacgcgaa actgggctt ctccggaag gccatttca aaaattagc actgaatac agactgcaa ttcgcttgg cctcagggc catacttca cgtagagat gccgtcgat tcctcggca cctcgcaaa tcccaatcg atgggagcg ctaaaaccg gccaccaat gcaatggtg cacaaaaaa cgcatcgat ttggaaaat aaggatgcc agtgaactc gaaccatta gtcgatgaa agaaacaca cgtgaagac aagactcac atattcaag gatgaattc aattccgaa tacaagaag aacttcaga cctttctcc cagtatgaa tactcggaa ggacgcttc accaaacgg ggagctccc gtcgatgac accgataac tcggccgga caaaactcg ctaccgcct caatgcgag aagaacgac tcttggtac agggaagtc gtcgaattg cgaaaaaag gcaggagaa tataagcac cgcggttgg ggtagtgaa cttgctcca gataggctt tctgatata tacaataag caagttgaa ctttgggat caagtttca cggagaagt tctctatca gccttatct ctcgcttcc atgacacac aagtcttat acaaaagaa gagaaggaa gtagataat aacaacaag acttctcca tcgaaagcg gctcgcaat gctgaaaac tcagctcga attgtacga gatatgatc agacatcat ttagaacga acaactggt ggttcagaa tttgacggt ttgattctg tcgccgact agagagaaa ctcgaaccg actatacca cgcaaagat gatgattcg agaagttcg caaaagaac agccccaag aaacactcg ccgatgaaa tcatcatct ttgaagcga cataaccag aaagccccc aaaagcggt cctaagaac ctacgttcc cagtctgtt ggtcctgtg actgatacg actgaaaaa cggtcacca aagcgccag tcacggtct gccaccgtc aaagagcga aaatcttcg gcaaatcaa gctagcatc aaaagacct cgaccttca tccttgaac actactgtc tcatcccga tctaaacat agctcggta ccctctaaa accgaagac gacagattg gccaaagct aattcaaaa tctaacact aagcaaaaa ctaggcact aaacagtta gaaaaagga aattctgga gccgaccca cccaagacc gaagctaac gaagaagtg aagaacgcc ccagtcgaa gcacccgaa ataatcgat tacgagccg gtggtaaag tcgccgccg gaaccgact agggtgaaa tctcccgaa caaattctg atgcgttcc ccggatcct gtcaactgg actgtgcca ctcgacacc ggaaaaact tttactgtc acacaaaac gtcagggag ggggatatg agtagtcga ccccatagc gaagtgaaa gcctggact cccccagac gtgcccctt cccatcgcc cagtctgct cctccgact cttgatcaa aataaggaa caaggcatg tcgtatgga tttagcagt gaaagcatg aactcgggc cagggcaga gtcatcgct gcacagctg agagacctc caaccaacg gtagagcat ctaaggcca cgttccatc gcagatgca tctttcaac agggtagta gccagaaag tgtcggaat ggccacggt tttgtaact ccgtggtgt atgttcgca aggactgaa caagggaca tacaccacg gcttcgcgc catttgctg aaccttatt tgagccgtt tgcccagct ttacctttg taatcaact gcttatgtt ccatgccat ccgtttaca aatccgtca aggtgccac atttcgttc gaaattatg gacgtcccc tttatacaa aaatttgtg atctctaat cagtgcact cgtgttttc tgccacaag gaagtcaga cgaaactat ctatggttg acatatatt catatattt tgtgtagat gcgtttgct tgccaactc tctggttaa tttagtgac atcgaacgc acttatgtt atttattat taatcatac agtaaagag ttatataaa agttt
