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Gene information
General information
TC005170 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4642273..4648190
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005170
    iBeetle screen iB_06595

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03914

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    4

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005170 RA
      MPVDKLHSE YRSTYRWHE YTGPRQEVV RRPPQPNAA 
      PNAGDTQHS SKEDEVHNE LPKLEPAMP RRKKYPDLA 
      YRHHEFLIS DGEITANGV CNTEPRARS EERGAHWRP 
      SRRSKSEGP RGGGGHGDG MRERERRRE SDPDLENMG 
      RETGLLRKA ISKISTEYR LQFAWPQGH TSRRDAVDS 
      SAPRKSQSM GALKPATNA MVHKKRIDL ENKDASELE 
      PLVDERNTR EDKTHIFKD EFNSEYKKN FRPFSQYEY 
      SEGRFTKRG APVDDTDNS AGQNSLPPQ CEKNDSWYR 
      EVVELRKKA GEYKHRGWG SELAPDRLS DIYNKQVEL 
      WDQVSRRSS LSALSLASM THKSYTKEE KEVDNNNKT 
      SPSKAARNA ENSARIVRD MIRHHLERT TGGSEFDGL 
      ILSPTREKL EPTIPRKDD DSRSSQKNS PKKHSPMKS 
      SSLKRHNQK APKSGPKNL RSQSVGPVT DTTEKRSPK 
      RQSRSATVK ERKSSANQA SIKRPRPSS LNTTVSSRS 
      KHSSVPSKT EDDRLAKAN SKSNTKQKL GTKQLEKGN 
      SGADPPKTE ANEEVKNAP VEAPEIIDY EPVVKSPPE 
      PTRVKSPEQ ILMRSPDPV NWTVPLDTG KTFTVTQNV 
      REGDMSSRP HSEVKAWTP PDVPLPIAQ SAPPTLDQN 
      KEQGMSYGF SSESMNSGQ GRVIAAQLR DLQPTVEHL 
      RPRSIADAS FNRVVARKC RNGHGFVTP WCMFARTEQ 
      GTYTTASRH LLNLI
    • >TC005170 RB
      MPVDKLHSE YRSTYRWHE YTGPRQEVV RRPPQPNAA 
      PNAVPKLEP AMPRRKKYP DLAYRHHEF LISDGEITA 
      NGVCNTEPR ARSEERGAH WRPSRRSKS EGPRGGGGH 
      GDGMRERER RRESDPDLE NMGRETGLL RKAISKIST 
      EYRLQFAWP QGHTSRRDA VDSSAPRKS QSMGALKPA 
      TNAMVHKKR IDLENKDAS ELEPLVDER NTREDKTHI 
      FKDEFNSEY KKNFRPFSQ YEYSEGRFT KRGAPVDDT 
      DNSAGQNSL PPQCEKNDS WYREVVELR KKAGEYKHR 
      GWGSELAPD RLSDIYNKQ VELWDQVSR RSSLSALSL 
      ASMTHKSYT KEEKEVDNN NKTSPSKAA RNAENSARI 
      VRDMIRHHL ERTTGGSEF DGLILSPTR EKLEPTIPR 
      KDDDSRSSQ KNSPKKHSP MKSSSLKRH NQKAPKSGP 
      KNLRSQSVG PVTDTTEKR SPKRQSRSA TVKERKSSA 
      NQASIKRPR PSSLNTTVS SRSKHSSVP SKTEDDRLA 
      KANSKSNTK QKLGTKQLE KGNSGADPP KTEANEEVK 
      NAPVEAPEI IDYEPVVKS PPEPTRVKS PEQILMRSP 
      DPVNWTVPL DTGKTFTVT QNVREGDMS SRPHSEVKA 
      WTPPDVPLP IAQSAPPTL DQNKEQGMS YGFSSESMN 
      SGQGRVIAA QLRDLQPTV EHLRPRSIA DASFNRVVA 
      RKCRNGHGF VTPWCMFAR TEQGTYTTA SRHLLNLI
    CDS sequence
    • >TC005170 RA
      atgcctgtc gataagctg cactccgag taccggagt 
      acttaccga tggcacgag tacacgggg cccaggcag 
      gaggtcgtg cggaggcca ccgcagccc aacgccgct 
      ccgaacgcc ggcgacact cagcactcc tccaaggag 
      gatgaggtg cataatgag ctgcccaag ctggaaccg 
      gcgatgcct aggaggaag aagtatccc gacttggcc 
      taccgccac cacgagttt ctgatcagt gatggcgaa 
      atcaccgcg aatggggtg tgcaacacg gaacctagg 
      gctcggtcg gaggagcgt ggggcgcac tggaggcct 
      tcccggcgg agcaagtcg gagggaccg cgtggcggc 
      ggcggccac ggggacggc atgcgggag cgggagcgg 
      cgtagggaa tcggacccg gatttggag aacatggga 
      cgcgaaact gggcttctc cggaaggcc atttcaaaa 
      attagcact gaatacaga ctgcaattc gcttggcct 
      cagggccat acttcacgt agagatgcc gtcgattcc 
      tcggcacct cgcaaatcc caatcgatg ggagcgcta 
      aaaccggcc accaatgca atggtgcac aaaaaacgc 
      atcgatttg gaaaataag gatgccagt gaactcgaa 
      ccattagtc gatgaaaga aacacacgt gaagacaag 
      actcacata ttcaaggat gaattcaat tccgaatac 
      aagaagaac ttcagacct ttctcccag tatgaatac 
      tcggaagga cgcttcacc aaacgggga gctcccgtc 
      gatgacacc gataactcg gccggacaa aactcgcta 
      ccgcctcaa tgcgagaag aacgactct tggtacagg 
      gaagtcgtc gaattgcga aaaaaggca ggagaatat 
      aagcaccgc ggttggggt agtgaactt gctccagat 
      aggctttct gatatatac aataagcaa gttgaactt 
      tgggatcaa gtttcacgg agaagttct ctatcagcc 
      ttatctctc gcttccatg acacacaag tcttataca 
      aaagaagag aaggaagta gataataac aacaagact 
      tctccatcg aaagcggct cgcaatgct gaaaactca 
      gctcgaatt gtacgagat atgatcaga catcattta 
      gaacgaaca actggtggt tcagaattt gacggtttg 
      attctgtcg ccgactaga gagaaactc gaaccgact 
      ataccacgc aaagatgat gattcgaga agttcgcaa 
      aagaacagc cccaagaaa cactcgccg atgaaatca 
      tcatctttg aagcgacat aaccagaaa gcccccaaa 
      agcggtcct aagaaccta cgttcccag tctgttggt 
      cctgtgact gatacgact gaaaaacgg tcaccaaag 
      cgccagtca cggtctgcc accgtcaaa gagcgaaaa 
      tcttcggca aatcaagct agcatcaaa agacctcga 
      ccttcatcc ttgaacact actgtctca tcccgatct 
      aaacatagc tcggtaccc tctaaaacc gaagacgac 
      agattggcc aaagctaat tcaaaatct aacactaag 
      caaaaacta ggcactaaa cagttagaa aaaggaaat 
      tctggagcc gacccaccc aagaccgaa gctaacgaa 
      gaagtgaag aacgcccca gtcgaagca cccgaaata 
      atcgattac gagccggtg gtaaagtcg ccgccggaa 
      ccgactagg gtgaaatct cccgaacaa attctgatg 
      cgttccccg gatcctgtc aactggact gtgccactc 
      gacaccgga aaaactttt actgtcaca caaaacgtc 
      agggagggg gatatgagt agtcgaccc catagcgaa 
      gtgaaagcc tggactccc ccagacgtg ccccttccc 
      atcgcccag tctgctcct ccgactctt gatcaaaat 
      aaggaacaa ggcatgtcg tatggattt agcagtgaa 
      agcatgaac tcgggccag ggcagagtc atcgctgca 
      cagctgaga gacctccaa ccaacggta gagcatcta 
      aggccacgt tccatcgca gatgcatct ttcaacagg 
      gtagtagcc agaaagtgt cggaatggc cacggtttt 
      gtaactccg tggtgtatg ttcgcaagg actgaacaa 
      gggacatac accacggct tcgcgccat ttgctgaac 
      cttatttga
    • >TC005170 RB
      atgcctgtc gataagctg cactccgag taccggagt 
      acttaccga tggcacgag tacacgggg cccaggcag 
      gaggtcgtg cggaggcca ccgcagccc aacgccgct 
      ccgaacgcc gtgcccaag ctggaaccg gcgatgcct 
      aggaggaag aagtatccc gacttggcc taccgccac 
      cacgagttt ctgatcagt gatggcgaa atcaccgcg 
      aatggggtg tgcaacacg gaacctagg gctcggtcg 
      gaggagcgt ggggcgcac tggaggcct tcccggcgg 
      agcaagtcg gagggaccg cgtggcggc ggcggccac 
      ggggacggc atgcgggag cgggagcgg cgtagggaa 
      tcggacccg gatttggag aacatggga cgcgaaact 
      gggcttctc cggaaggcc atttcaaaa attagcact 
      gaatacaga ctgcaattc gcttggcct cagggccat 
      acttcacgt agagatgcc gtcgattcc tcggcacct 
      cgcaaatcc caatcgatg ggagcgcta aaaccggcc 
      accaatgca atggtgcac aaaaaacgc atcgatttg 
      gaaaataag gatgccagt gaactcgaa ccattagtc 
      gatgaaaga aacacacgt gaagacaag actcacata 
      ttcaaggat gaattcaat tccgaatac aagaagaac 
      ttcagacct ttctcccag tatgaatac tcggaagga 
      cgcttcacc aaacgggga gctcccgtc gatgacacc 
      gataactcg gccggacaa aactcgcta ccgcctcaa 
      tgcgagaag aacgactct tggtacagg gaagtcgtc 
      gaattgcga aaaaaggca ggagaatat aagcaccgc 
      ggttggggt agtgaactt gctccagat aggctttct 
      gatatatac aataagcaa gttgaactt tgggatcaa 
      gtttcacgg agaagttct ctatcagcc ttatctctc 
      gcttccatg acacacaag tcttataca aaagaagag 
      aaggaagta gataataac aacaagact tctccatcg 
      aaagcggct cgcaatgct gaaaactca gctcgaatt 
      gtacgagat atgatcaga catcattta gaacgaaca 
      actggtggt tcagaattt gacggtttg attctgtcg 
      ccgactaga gagaaactc gaaccgact ataccacgc 
      aaagatgat gattcgaga agttcgcaa aagaacagc 
      cccaagaaa cactcgccg atgaaatca tcatctttg 
      aagcgacat aaccagaaa gcccccaaa agcggtcct 
      aagaaccta cgttcccag tctgttggt cctgtgact 
      gatacgact gaaaaacgg tcaccaaag cgccagtca 
      cggtctgcc accgtcaaa gagcgaaaa tcttcggca 
      aatcaagct agcatcaaa agacctcga ccttcatcc 
      ttgaacact actgtctca tcccgatct aaacatagc 
      tcggtaccc tctaaaacc gaagacgac agattggcc 
      aaagctaat tcaaaatct aacactaag caaaaacta 
      ggcactaaa cagttagaa aaaggaaat tctggagcc 
      gacccaccc aagaccgaa gctaacgaa gaagtgaag 
      aacgcccca gtcgaagca cccgaaata atcgattac 
      gagccggtg gtaaagtcg ccgccggaa ccgactagg 
      gtgaaatct cccgaacaa attctgatg cgttccccg 
      gatcctgtc aactggact gtgccactc gacaccgga 
      aaaactttt actgtcaca caaaacgtc agggagggg 
      gatatgagt agtcgaccc catagcgaa gtgaaagcc 
      tggactccc ccagacgtg ccccttccc atcgcccag 
      tctgctcct ccgactctt gatcaaaat aaggaacaa 
      ggcatgtcg tatggattt agcagtgaa agcatgaac 
      tcgggccag ggcagagtc atcgctgca cagctgaga 
      gacctccaa ccaacggta gagcatcta aggccacgt 
      tccatcgca gatgcatct ttcaacagg gtagtagcc 
      agaaagtgt cggaatggc cacggtttt gtaactccg 
      tggtgtatg ttcgcaagg actgaacaa gggacatac 
      accacggct tcgcgccat ttgctgaac cttatttga
    mRNA sequence
    • >TC005170 RA
      aaacgctcg agatttgac gaattttgt tgccacacg 
      acggcgtca gcccgcatt gctgtggct taattaatt 
      atgcaaggg agctttcgt gttcacttt ttattcaaa 
      agccccaaa tgtcgagta tgacgtcgc tggaagaaa 
      atttttggt tcgcattaa tcaaccggc gactgcact 
      caacagatt cagaagtgc aacgctatg ttgcattat 
      gttgtgcgg atgtgaaaa agcaatgca atttacggt 
      tttctgtgt taaatccaa aaaaccgta actggcatg 
      aacccgttt aggaaaagt tgcacttgc attattcat 
      aatttagga atccatttt ctagaatat gggagcagt 
      taacattgt tttattaca tacagataa ggttttata 
      atcgggatc gtctgcctg gaaaacaaa cgatatttt 
      attcatgat ctgtattct aatgcgtcg gattaacga 
      aacgttcat gtttttatt ttgtgttgt attaggagg 
      gcgggtgct aaaaccatg gaggttttt catgatgtg 
      gtttgaagc aaatttgga tcgggtggt agtaaagcg 
      ttcagtagt ctatgcttc attataatt agtaaattt 
      ggttaaaat ggtatagcc gactatgca atcgatagt 
      cgctttatt ggtcgtatt gtgtgacct ttgaaagtc 
      aggtttgta gttaaaaca gccttaaat gagcaaatg 
      tcaatggga tagattagg tggcattat ttaatttcc 
      cttgtaatg gtttttcct ttgtttaag cgagtggag 
      tcgagtaaa attggattt aaaagttgt gtgtctttg 
      aaaaagttt aatttgttg tatgatgtt agaaattta 
      atttgtgtg ttacataaa tgatattgc ctgtccggg 
      gtctggaat gaataaaat gtctcggga agaattttg 
      cagggttgg gaaaaatgt gaggaaagg gggtggtag 
      gtcagagat gatgtctac tgaccgatc gtccttgat 
      tcccccatt ttggggtga atgacctcg cacattgaa 
      tttccctgt gagcgtgtt gggggtgag ggctgaata 
      tcgttttgc aggtgattt cgctgctat tgaacgaat 
      tggaattgg agtaatagg aatcgagtc cggtgataa 
      taattcggg gtatttgtt gtggatggc gctctaagt 
      gggtaaaaa acagtagcg caactggcg caacgatag 
      aattgcaag gagcaggcg cccgtctat tgattctta 
      ggttgagta gagccgtcg tcgacccga ccacggatt 
      gccaataag gtggggctc ctgtcatgc tgcacgagc 
      tctcgtgtc cgtcgagtc tggtggctc attaagttc 
      tcgcgacct acgccgtct cctctcacg ttttctggc 
      ccccggagt ttccggtgc ggtgtatgg cttgtcggc 
      ctcagagtt acttggaga gaatttaag tgcccattc 
      gacctgatg attggctca ttctggaac ttgtgcaga 
      gcttgcccg tcgatgcct gtcgataag ctgcactcc 
      gagtaccgg agtacttac cgatggcac gagtacacg 
      gggcccagg caggaggtc gtgcggagg ccaccgcag 
      cccaacgcc gctccgaac gccggcgac actcagcac 
      tcctccaag gaggatgag gtgcataat gagctgccc 
      aagctggaa ccggcgatg cctaggagg aagaagtat 
      cccgacttg gcctaccgc caccacgag tttctgatc 
      agtgatggc gaaatcacc gcgaatggg gtgtgcaac 
      acggaacct agggctcgg tcggaggag cgtggggcg 
      cactggagg ccttcccgg cggagcaag tcggaggga 
      ccgcgtggc ggcggcggc cacggggac ggcatgcgg 
      gagcgggag cggcgtagg gaatcggac ccggatttg 
      gagaacatg ggacgcgaa actgggctt ctccggaag 
      gccatttca aaaattagc actgaatac agactgcaa 
      ttcgcttgg cctcagggc catacttca cgtagagat 
      gccgtcgat tcctcggca cctcgcaaa tcccaatcg 
      atgggagcg ctaaaaccg gccaccaat gcaatggtg 
      cacaaaaaa cgcatcgat ttggaaaat aaggatgcc 
      agtgaactc gaaccatta gtcgatgaa agaaacaca 
      cgtgaagac aagactcac atattcaag gatgaattc 
      aattccgaa tacaagaag aacttcaga cctttctcc 
      cagtatgaa tactcggaa ggacgcttc accaaacgg 
      ggagctccc gtcgatgac accgataac tcggccgga 
      caaaactcg ctaccgcct caatgcgag aagaacgac 
      tcttggtac agggaagtc gtcgaattg cgaaaaaag 
      gcaggagaa tataagcac cgcggttgg ggtagtgaa 
      cttgctcca gataggctt tctgatata tacaataag 
      caagttgaa ctttgggat caagtttca cggagaagt 
      tctctatca gccttatct ctcgcttcc atgacacac 
      aagtcttat acaaaagaa gagaaggaa gtagataat 
      aacaacaag acttctcca tcgaaagcg gctcgcaat 
      gctgaaaac tcagctcga attgtacga gatatgatc 
      agacatcat ttagaacga acaactggt ggttcagaa 
      tttgacggt ttgattctg tcgccgact agagagaaa 
      ctcgaaccg actatacca cgcaaagat gatgattcg 
      agaagttcg caaaagaac agccccaag aaacactcg 
      ccgatgaaa tcatcatct ttgaagcga cataaccag 
      aaagccccc aaaagcggt cctaagaac ctacgttcc 
      cagtctgtt ggtcctgtg actgatacg actgaaaaa 
      cggtcacca aagcgccag tcacggtct gccaccgtc 
      aaagagcga aaatcttcg gcaaatcaa gctagcatc 
      aaaagacct cgaccttca tccttgaac actactgtc 
      tcatcccga tctaaacat agctcggta ccctctaaa 
      accgaagac gacagattg gccaaagct aattcaaaa 
      tctaacact aagcaaaaa ctaggcact aaacagtta 
      gaaaaagga aattctgga gccgaccca cccaagacc 
      gaagctaac gaagaagtg aagaacgcc ccagtcgaa 
      gcacccgaa ataatcgat tacgagccg gtggtaaag 
      tcgccgccg gaaccgact agggtgaaa tctcccgaa 
      caaattctg atgcgttcc ccggatcct gtcaactgg 
      actgtgcca ctcgacacc ggaaaaact tttactgtc 
      acacaaaac gtcagggag ggggatatg agtagtcga 
      ccccatagc gaagtgaaa gcctggact cccccagac 
      gtgcccctt cccatcgcc cagtctgct cctccgact 
      cttgatcaa aataaggaa caaggcatg tcgtatgga 
      tttagcagt gaaagcatg aactcgggc cagggcaga 
      gtcatcgct gcacagctg agagacctc caaccaacg 
      gtagagcat ctaaggcca cgttccatc gcagatgca 
      tctttcaac agggtagta gccagaaag tgtcggaat 
      ggccacggt tttgtaact ccgtggtgt atgttcgca 
      aggactgaa caagggaca tacaccacg gcttcgcgc 
      catttgctg aaccttatt tgagccgtt tgcccagct 
      ttacctttg taatcaact gcttatgtt ccatgccat 
      ccgtttaca aatccgtca aggtgccac atttcgttc 
      gaaattatg gacgtcccc tttatacaa aaatttgtg 
      atctctaat cagtgcact cgtgttttc tgccacaag 
      gaagtcaga cgaaactat ctatggttg acatatatt 
      catatattt tgtgtagat gcgtttgct tgccaactc 
      tctggttaa tttagtgac atcgaacgc acttatgtt 
      atttattat taatcatac agtaaagag ttatataaa 
      agttt
    • >TC005170 RB
      aaacgctcg agatttgac gaattttgt tgccacacg 
      acggcgtca gcccgcatt gctgtggct taattaatt 
      atgcaaggg agctttcgt gttcacttt ttattcaaa 
      agccccaaa tgtcgagta tgacgtcgc tggaagaaa 
      atttttggt tcgcattaa tcaaccggc gactgcact 
      caacagatt cagaagtgc aacgctatg ttgcattat 
      gttgtgcgg atgtgaaaa agcaatgca atttacggt 
      tttctgtgt taaatccaa aaaaccgta actggcatg 
      aacccgttt aggaaaagt tgcacttgc attattcat 
      aatttagga atccatttt ctagaatat gggagcagt 
      taacattgt tttattaca tacagataa ggttttata 
      atcgggatc gtctgcctg gaaaacaaa cgatatttt 
      attcatgat ctgtattct aatgcgtcg gattaacga 
      aacgttcat gtttttatt ttgtgttgt attaggagg 
      gcgggtgct aaaaccatg gaggttttt catgatgtg 
      gtttgaagc aaatttgga tcgggtggt agtaaagcg 
      ttcagtagt ctatgcttc attataatt agtaaattt 
      ggttaaaat ggtatagcc gactatgca atcgatagt 
      cgctttatt ggtcgtatt gtgtgacct ttgaaagtc 
      aggtttgta gttaaaaca gccttaaat gagcaaatg 
      tcaatggga tagattagg tggcattat ttaatttcc 
      cttgtaatg gtttttcct ttgtttaag cgagtggag 
      tcgagtaaa attggattt aaaagttgt gtgtctttg 
      aaaaagttt aatttgttg tatgatgtt agaaattta 
      atttgtgtg ttacataaa tgatattgc ctgtccggg 
      gtctggaat gaataaaat gtctcggga agaattttg 
      cagggttgg gaaaaatgt gaggaaagg gggtggtag 
      gtcagagat gatgtctac tgaccgatc gtccttgat 
      tcccccatt ttggggtga atgacctcg cacattgaa 
      tttccctgt gagcgtgtt gggggtgag ggctgaata 
      tcgttttgc aggtgattt cgctgctat tgaacgaat 
      tggaattgg agtaatagg aatcgagtc cggtgataa 
      taattcggg gtatttgtt gtggatggc gctctaagt 
      gggtaaaaa acagtagcg caactggcg caacgatag 
      aattgcaag gagcaggcg cccgtctat tgattctta 
      ggttgagta gagccgtcg tcgacccga ccacggatt 
      gccaataag gtggggctc ctgtcatgc tgcacgagc 
      tctcgtgtc cgtcgagtc tggtggctc attaagttc 
      tcgcgacct acgccgtct cctctcacg ttttctggc 
      ccccggagt ttccggtgc ggtgtatgg cttgtcggc 
      ctcagagtt acttggaga gaatttaag tgcccattc 
      gacctgatg attggctca ttctggaac ttgtgcaga 
      gcttgcccg tcgatgcct gtcgataag ctgcactcc 
      gagtaccgg agtacttac cgatggcac gagtacacg 
      gggcccagg caggaggtc gtgcggagg ccaccgcag 
      cccaacgcc gctccgaac gccgtgccc aagctggaa 
      ccggcgatg cctaggagg aagaagtat cccgacttg 
      gcctaccgc caccacgag tttctgatc agtgatggc 
      gaaatcacc gcgaatggg gtgtgcaac acggaacct 
      agggctcgg tcggaggag cgtggggcg cactggagg 
      ccttcccgg cggagcaag tcggaggga ccgcgtggc 
      ggcggcggc cacggggac ggcatgcgg gagcgggag 
      cggcgtagg gaatcggac ccggatttg gagaacatg 
      ggacgcgaa actgggctt ctccggaag gccatttca 
      aaaattagc actgaatac agactgcaa ttcgcttgg 
      cctcagggc catacttca cgtagagat gccgtcgat 
      tcctcggca cctcgcaaa tcccaatcg atgggagcg 
      ctaaaaccg gccaccaat gcaatggtg cacaaaaaa 
      cgcatcgat ttggaaaat aaggatgcc agtgaactc 
      gaaccatta gtcgatgaa agaaacaca cgtgaagac 
      aagactcac atattcaag gatgaattc aattccgaa 
      tacaagaag aacttcaga cctttctcc cagtatgaa 
      tactcggaa ggacgcttc accaaacgg ggagctccc 
      gtcgatgac accgataac tcggccgga caaaactcg 
      ctaccgcct caatgcgag aagaacgac tcttggtac 
      agggaagtc gtcgaattg cgaaaaaag gcaggagaa 
      tataagcac cgcggttgg ggtagtgaa cttgctcca 
      gataggctt tctgatata tacaataag caagttgaa 
      ctttgggat caagtttca cggagaagt tctctatca 
      gccttatct ctcgcttcc atgacacac aagtcttat 
      acaaaagaa gagaaggaa gtagataat aacaacaag 
      acttctcca tcgaaagcg gctcgcaat gctgaaaac 
      tcagctcga attgtacga gatatgatc agacatcat 
      ttagaacga acaactggt ggttcagaa tttgacggt 
      ttgattctg tcgccgact agagagaaa ctcgaaccg 
      actatacca cgcaaagat gatgattcg agaagttcg 
      caaaagaac agccccaag aaacactcg ccgatgaaa 
      tcatcatct ttgaagcga cataaccag aaagccccc 
      aaaagcggt cctaagaac ctacgttcc cagtctgtt 
      ggtcctgtg actgatacg actgaaaaa cggtcacca 
      aagcgccag tcacggtct gccaccgtc aaagagcga 
      aaatcttcg gcaaatcaa gctagcatc aaaagacct 
      cgaccttca tccttgaac actactgtc tcatcccga 
      tctaaacat agctcggta ccctctaaa accgaagac 
      gacagattg gccaaagct aattcaaaa tctaacact 
      aagcaaaaa ctaggcact aaacagtta gaaaaagga 
      aattctgga gccgaccca cccaagacc gaagctaac 
      gaagaagtg aagaacgcc ccagtcgaa gcacccgaa 
      ataatcgat tacgagccg gtggtaaag tcgccgccg 
      gaaccgact agggtgaaa tctcccgaa caaattctg 
      atgcgttcc ccggatcct gtcaactgg actgtgcca 
      ctcgacacc ggaaaaact tttactgtc acacaaaac 
      gtcagggag ggggatatg agtagtcga ccccatagc 
      gaagtgaaa gcctggact cccccagac gtgcccctt 
      cccatcgcc cagtctgct cctccgact cttgatcaa 
      aataaggaa caaggcatg tcgtatgga tttagcagt 
      gaaagcatg aactcgggc cagggcaga gtcatcgct 
      gcacagctg agagacctc caaccaacg gtagagcat 
      ctaaggcca cgttccatc gcagatgca tctttcaac 
      agggtagta gccagaaag tgtcggaat ggccacggt 
      tttgtaact ccgtggtgt atgttcgca aggactgaa 
      caagggaca tacaccacg gcttcgcgc catttgctg 
      aaccttatt tgagccgtt tgcccagct ttacctttg 
      taatcaact gcttatgtt ccatgccat ccgtttaca 
      aatccgtca aggtgccac atttcgttc gaaattatg 
      gacgtcccc tttatacaa aaatttgtg atctctaat 
      cagtgcact cgtgttttc tgccacaag gaagtcaga 
      cgaaactat ctatggttg acatatatt catatattt 
      tgtgtagat gcgtttgct tgccaactc tctggttaa 
      tttagtgac atcgaacgc acttatgtt atttattat 
      taatcatac agtaaagag ttatataaa agttt
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