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Gene information
General information
TC004635 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4232411..4235139
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004635
    iBeetle screen iB_06593

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004635 RA
      MGIIYQLFY LCLLIVVFG FSSLAAQQH PEASDAIVI 
      EAKSERQPK RSDSQFVLK ETAYQYNPG LIPSNINTV 
      PLATTGPCF TGKGVLGKC TSFRQCYPY FKVPVLDNF 
      ESWILGMYD TCSYYTAQG RQMFGVCCA NPVKPATEP 
      SENFNNALT SSNTTINEA IPYYPQLTN WPPPIPTHP 
      PDHTIPPLP THPPSPGYP PLPTHPPSP GYPTLPPVT 
      QKPPAWPPA ITTKPPYKP TRPTTKPPA TQAPVVSSC 
      GAKNGYQDQ ERIVGGHNA DVGEWPWIA ALFNGGRQF 
      CGGSLIDNI HILSAAHCV AHMSSWDVA RLTVRLGDH 
      NIKTNTEIR HIEKRVKRI VRHRGFDPR TLYNDIAIL 
      TLDSPVQFS QQIRPICLP TVGNDFAGH TGTVIGWGS 
      LRESGPQPS VLQEVNIPI WSNRDCKLK YGPAAPGGI 
      VDHMLCAGQ AARDSCSGD SGGPLMVNS GKWTQVGIV 
      SWGIGCGKG QYPGVYTRV EKFLPWINK NLK
    CDS sequence
    • >TC004635 RA
      atggggata atttaccaa ttattttat ttgtgttta 
      ttgattgtt gtgtttggt ttctcatct ctcgccgct 
      cagcagcat ccagaagca tcagatgca attgtgatt 
      gaagctaaa tcagagaga caacccaaa aggagcgac 
      agtcagttt gttttgaaa gaaacagcg tatcaatac 
      aatcccggt ttaattccg tctaacatt aacacggtc 
      cctttggca acaacaggc ccttgtttt actggcaaa 
      ggagttctc gggaaatgc acttcattt cgccaatgc 
      tatccctac ttcaaagtg ccagtatta gacaatttc 
      gaaagctgg attttagga atgtacgac acttgcagt 
      tattacact gcacaaggt agacagatg tttggggtc 
      tgctgcgca aacccagtc aaaccagca actgagccg 
      agcgaaaac ttcaataac gctctgacc agctcaaat 
      actaccatt aatgaggcc attccgtat tacccacag 
      ttgaccaac tggccgccc ccgatcccg acccacccc 
      ccagaccac accattccg ccgttgccc acccatccc 
      ccatctccc ggatacccg ccgttgccg acccaccct 
      ccctctccc ggatatccc accctgccc cctgtcacc 
      cagaagccg ccagcgtgg cccccggcc ataaccacc 
      aagccccca tacaagccc acgagaccc acaaccaag 
      ccgccagcg acgcaggca cccgtcgtg tcttcttgt 
      ggggcgaaa aatgggtac caagatcaa gaacggatt 
      gtgggtgga cataacgcg gacgtcggt gaatggcct 
      tggattgcg gcacttttt aacggagga agacagttt 
      tgtggaggc tccttgatt gataatatc cacatatta 
      tcggcagcc cattgcgta gcccacatg agctcttgg 
      gacgtcgca agactaacg gttagactg ggagaccac 
      aacattaaa accaatacg gaaatcaga cacattgaa 
      aagcgcgtt aaaaggatt gtgagacat agaggcttt 
      gatcctcgg acattgtac aacgacatt gcgattttg 
      accttagat agtccagtt cagttctcg caacaaatc 
      aggccaatt tgtttacca acagtaggt aatgatttt 
      gcaggacac acaggcacg gtgattgga tggggaagt 
      ctcagggaa agtggacca caaccttcc gtattacaa 
      gaagttaat attcccatc tggagtaat agagactgt 
      aaattaaaa tatggtcct gcggcacct ggaggcatt 
      gtggaccac atgttatgt gcaggacaa gctgccaga 
      gattcttgc agcggtgat agtggaggt cccctcatg 
      gttaattca ggaaagtgg actcaagtt ggaatcgtt 
      tcttgggga atagggtgc ggtaaagga caatatcca 
      ggggtttac acccgagtg gaaaagttt ttgccatgg 
      ataaataaa aatttaaaa tga
    mRNA sequence
    • >TC004635 RA
      tgtgagaca gtaacgtat catcaccac gcaatgggg 
      ataatttac caattattt tatttgtgt ttattgatt 
      gttgtgttt ggtttctca tctctcgcc gctcagcag 
      catccagaa gcatcagat gcaattgtg attgaagct 
      aaatcagag agacaaccc aaaaggagc gacagtcag 
      tttgttttg aaagaaaca gcgtatcaa tacaatccc 
      ggtttaatt ccgtctaac attaacacg gtccctttg 
      gcaacaaca ggcccttgt tttactggc aaaggagtt 
      ctcgggaaa tgcacttca tttcgccaa tgctatccc 
      tacttcaaa gtgccagta ttagacaat ttcgaaagc 
      tggatttta ggaatgtac gacacttgc agttattac 
      actgcacaa ggtagacag atgtttggg gtctgctgc 
      gcaaaccca gtcaaacca gcaactgag ccgagcgaa 
      aacttcaat aacgctctg accagctca aatactacc 
      attaatgag gccattccg tattaccca cagttgacc 
      aactggccg cccccgatc ccgacccac cccccagac 
      cacaccatt ccgccgttg cccacccat cccccatct 
      cccggatac ccgccgttg ccgacccac cctccctct 
      cccggatat cccaccctg ccccctgtc acccagaag 
      ccgccagcg tggcccccg gccataacc accaagccc 
      ccatacaag cccacgaga cccacaacc aagccgcca 
      gcgacgcag gcacccgtc gtgtcttct tgtggggcg 
      aaaaatggg taccaagat caagaacgg attgtgggt 
      ggacataac gcggacgtc ggtgaatgg ccttggatt 
      gcggcactt tttaacgga ggaagacag ttttgtgga 
      ggctccttg attgataat atccacata ttatcggca 
      gcccattgc gtagcccac atgagctct tgggacgtc 
      gcaagacta acggttaga ctgggagac cacaacatt 
      aaaaccaat acggaaatc agacacatt gaaaagcgc 
      gttaaaagg attgtgaga catagaggc tttgatcct 
      cggacattg tacaacgac attgcgatt ttgacctta 
      gatagtcca gttcagttc tcgcaacaa atcaggcca 
      atttgttta ccaacagta ggtaatgat tttgcagga 
      cacacaggc acggtgatt ggatgggga agtctcagg 
      gaaagtgga ccacaacct tccgtatta caagaagtt 
      aatattccc atctggagt aatagagac tgtaaatta 
      aaatatggt cctgcggca cctggaggc attgtggac 
      cacatgtta tgtgcagga caagctgcc agagattct 
      tgcagcggt gatagtgga ggtcccctc atggttaat 
      tcaggaaag tggactcaa gttggaatc gtttcttgg 
      ggaataggg tgcggtaaa ggacaatat ccaggggtt 
      tacacccga gtggaaaag tttttgcca tggataaat 
      aaaaattta aaatgatct gtatcaaat aaaagtttt 
      ttaataag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY