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Gene information
General information
TC004722 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4271787..4273675
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004722
    iBeetle screen iB_06592

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004722 RA
      MYGNPGSGY QGYPGGAAP GYGQGAATP 
      DVQQWFNTV DKDRSGQIN WQELQSALI 
      NGQGKNFSD VACKLMIGM FDRDKTGTI 
      DINEFQQLF AYINQWLAV FKNYDRDQS 
      GHIEEPELA QALQQMGFK FSPDFVKFL 
      IAKSDLQNH KQMSVDQFI VLCVQIQRF 
      TEAFRSRDS EMKGVITIG FEDFLSVAI 
      NCST
    CDS sequence
    • >TC004722 RA
      atgtacggt aatcccggg agtggatac caaggatat 
      cctggtggg gctgcccct gggtacggt caaggagct 
      gcaactccg gatgttcag cagtggttt aatacagta 
      gataaggat cgttctggt caaataaat tggcaagaa 
      ctacaaagt gctcttatc aatggccag gggaagaat 
      ttttcagac gtcgcttgc aagcttatg attgggatg 
      tttgatagg gataagaca gggacaatt gatattaac 
      gaattccaa caactcttc gcatacatt aatcaatgg 
      ttggctgta tttaaaaat tatgatagg gaccagtca 
      ggacacatt gaggagcca gaacttgcc caagcgttg 
      caacagatg ggatttaaa ttttccccc gatttcgta 
      aaattttta attgctaaa agcgacctt caaaaccac 
      aaacaaatg tcagtagac caatttatc gtactttgc 
      gtgcaaatc cagcggttc actgaagct ttccgatca 
      agggacagt gaaatgaag ggggttatt acaattgga 
      tttgaagat tttctcagt gtcgcgata aactgttcc 
      acatga
    mRNA sequence
    • >TC004722 RA
      ctagaagcg ttgttactg actgttacc atagaataa 
      gaggttaac agttgacag ttcttggtg attatttgt 
      ttatataaa tgtgcgtta atttctaat atttttttg 
      taattgtac atgccacaa gcataatgt acggtaatc 
      ccgggagtg gataccaag gatatcctg gtggggctg 
      cccctgggt acggtcaag gagctgcaa ctccggatg 
      ttcagcagt ggtttaata cagtagata aggatcgtt 
      ctggtcaaa taaattggc aagaactac aaagtgctc 
      ttatcaatg gccagggga agaattttt cagacgtcg 
      cttgcaagc ttatgattg ggatgtttg atagggata 
      agacaggga caattgata ttaacgaat tccaacaac 
      tcttcgcat acattaatc aatggttgg ctgtattta 
      aaaattatg atagggacc agtcaggac acattgagg 
      agccagaac ttgcccaag cgttgcaac agatgggat 
      ttaaatttt cccccgatt tcgtaaaat ttttaattg 
      ctaaaagcg accttcaaa accacaaac aaatgtcag 
      tagaccaat ttatcgtac tttgcgtgc aaatccagc 
      ggttcactg aagctttcc gatcaaggg acagtgaaa 
      tgaaggggg ttattacaa ttggatttg aagattttc 
      tcagtgtcg cgataaact gttccacat gatttacta 
      tttaattcg tttaattcc attactagg gttaatttt 
      gtaggaaca ataaaacta ataaaactt agtctctaa 
      aatgtttta ttaaaaatc ccgtacagt atagatgtt 
      ccacatcat ttttaagca aacaaatac ttattgtaa 
      caacagtag gcaatgggt cgggtcaaa aaatattac 
      tgtcgtatc tataaatta atcacatta ttttacgta 
      catttttaa atagctaaa ttttatagt atagtctac 
      tgattaaaa gtgaatttc agcagcaca tttttaaag 
      tcgttgatt gtttgttag gcgtttttt tctacttga 
      tattataaa aacgttagg attatagtg tggtcgttg 
      taagtacaa gttgccgtt aaaattcac ttgtgaaac 
      gtaactgaa ttaatctac aagcattgg ccaatgtcc 
      ctgtaaagt ttcagtata aaacgtcga aacaaatca 
      cagctaaaa catgctaca gcaaatttt aatcctaag 
      tgaagaagt acaaacttt tcaaatata tttttatat 
      taataagcg tcattattg tgccctaag ttcagtaca 
      tgttaaaaa tattcgcaa gttttaggt ccaaaaatc 
      tataatcat ttacattat tttgaatat ttaacggtc 
      ataatatat aaactaata acaaattta aattatgtc 
      atacatcgt cttttaaaa atttgtttt ttgattgtt 
      gattagttt tattatcat aaaattctt ctctggtgg 
      tttctaaag tcgttggcg tgatgagta tttctgttg 
      atttcgaac ggcatagta acggcttct ccgctgcgt 
      cgtcttcat cttcttcac aatttcgat ttctcgtta 
      actttttca cccctgtag agaaaaacc ggtgttaat 
      cacttggca atttgtgca gttgcgcta caggatttg 
      ccattacag acgaatata ctctacaac ggttactat 
      agggccaag aacagattt caacatcga gaataaata 
      ttaaatatc atg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY