Status
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Gene information
General information
TC034524 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4491441..4500288
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034524
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06589

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    remarks: eggs lost,
    Adult morphology
    remarks: eggs lost,
    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    vitellarium not present - Number of animals showing the phenotype: 4
    tropharium shortened - Number of animals showing the phenotype: 4

    Comment: also empty. potentially due to preperation
    Analysis of larval stage 1 cuticle
    remarks: eggs lost,
    remarks - cuticles: later than schedule,
     
    8 days later, due to lost eggs in day 9
     

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034524 RA
      MMSNGLDSG VVGQQNGGS GGQVGGGQE ESKTNLIVN 
      YLPQTMTQE EIRSLFSSI GEVESCKLI RDKVTGQSL 
      GYGFVNYHR PEDAEKAIN TLNGLRLQN KTIKVSYAR 
      PSSEAIKGA NLYVSGLPK NMTQQDLES LFSPYGRII 
      TSRILCDNI TVRQFVTGA GENLPGLSK GVGFIRFDQ 
      RLEAERAIQ ELNGTIPKG STEPITVKF ANNPSNNNK 
      AIPPLAAYL TPQATRRFA GPIHHPTGR FRYSPLAGD 
      LLANSMLPG NAMNGSGWC IFVYNLAPE TEENVLWQL 
      FGPFGAVQS VKVIRDLQT NKCKGFGFV TMTNYDEAV 
      VAIQSLNGY TLGNRVLQV SFKTNKSKT T
    • >TC034524 RB
      MMSNGLDSG VVGQQNGGS GGQVGGGQE ESKTNLIVN 
      YLPQTMTQE EIRSLFSSI GEVESCKLI RDKVTGQSL 
      GYGFVNYHR PEDAEKAIN TLNGLRLQN KTIKVSYAR 
      PSSEAIKGA NLYVSGLPK NMTQQDLES LFSPYGRII 
      TSRILCDNI TGLSKGVGF IRFDQRLEA ERAIQELNG 
      TIPKGSTEP ITVKFANNP SNNNKAIPP LAAYLTPQA 
      TRRFAGPIH HPTGRFRYS PLAGDLLAN SMLPGNAMN 
      GSGWCIFVY NLAPETEEN VLWQLFGPF GAVQSVKVI 
      RDLQTNKCK GFGFVTMTN YDEAVVAIQ SLNGYTLGN 
      RVLQVSFKT NKSKTT
    CDS sequence
    • >TC034524 RA
      atgatgtcc aacggatta gactcgggg gttgttgga 
      caacagaat ggaggcagc gggggccaa gtcggtggg 
      ggtcaagaa gagtccaaa acgaatctc atcgttaat 
      tacctaccc caaaccatg acacaagaa gaaatccga 
      tctttattt tccagtatt ggagaagtc gaaagttgc 
      aaactaatc cgcgataaa gtcacaggg caatcactt 
      ggttatggc ttcgtaaac taccaccgg ccagaagat 
      gcagaaaaa gcaattaat acattgaac ggattaaga 
      ttgcagaac aagacaatt aaggtgtca tatgcacgg 
      ccaagttcc gaagcaatc aagggtgca aacctgtac 
      gtttccgga ttaccgaag aacatgacc caacaagat 
      ttggaatcc ctgtttagt ccgtacggt cgtataata 
      acatcgcgc attttatgc gacaacata accgtacgt 
      caatttgtc acgggggcc ggagagaat ttgcctggc 
      ctgtcgaaa ggagtaggt ttcatccgt tttgaccaa 
      cgtttagaa gcagaacga gcaattcaa gaactcaat 
      ggtaccatt cctaagggc tccaccgaa cctataaca 
      gtgaaattt gccaacaac ccgagtaac aacaacaaa 
      gccattcct ccattggca gcttatcta actccccaa 
      gccactcgc cgtttcgct ggccctatc caccaccca 
      acgggacga ttcaggtat tctccgctg gccggagac 
      ctgctggcc aactccatg ctacccgga aacgctatg 
      aacggttcc ggttggtgc attttcgtg tataacctc 
      gctccagag acagaagag aatgttctc tggcagttg 
      tttggcccg ttcggagcc gtccagagc gtcaaagtg 
      atccgtgac ctgcagacg aacaaatgt aaaggtttc 
      ggctttgtc acgatgacc aattatgac gaagccgtg 
      gtagccatc cagtcgcta aacggttac acgctgggt 
      aaccgtgtc ctgcaggtc agctttaaa acgaataaa 
      agtaaaact acatag
    • >TC034524 RB
      atgatgtcc aacggatta gactcgggg gttgttgga 
      caacagaat ggaggcagc gggggccaa gtcggtggg 
      ggtcaagaa gagtccaaa acgaatctc atcgttaat 
      tacctaccc caaaccatg acacaagaa gaaatccga 
      tctttattt tccagtatt ggagaagtc gaaagttgc 
      aaactaatc cgcgataaa gtcacaggg caatcactt 
      ggttatggc ttcgtaaac taccaccgg ccagaagat 
      gcagaaaaa gcaattaat acattgaac ggattaaga 
      ttgcagaac aagacaatt aaggtgtca tatgcacgg 
      ccaagttcc gaagcaatc aagggtgca aacctgtac 
      gtttccgga ttaccgaag aacatgacc caacaagat 
      ttggaatcc ctgtttagt ccgtacggt cgtataata 
      acatcgcgc attttatgc gacaacata accggcctg 
      tcgaaagga gtaggtttc atccgtttt gaccaacgt 
      ttagaagca gaacgagca attcaagaa ctcaatggt 
      accattcct aagggctcc accgaacct ataacagtg 
      aaatttgcc aacaacccg agtaacaac aacaaagcc 
      attcctcca ttggcagct tatctaact ccccaagcc 
      actcgccgt ttcgctggc cctatccac cacccaacg 
      ggacgattc aggtattct ccgctggcc ggagacctg 
      ctggccaac tccatgcta cccggaaac gctatgaac 
      ggttccggt tggtgcatt ttcgtgtat aacctcgct 
      ccagagaca gaagagaat gttctctgg cagttgttt 
      ggcccgttc ggagccgtc cagagcgtc aaagtgatc 
      cgtgacctg cagacgaac aaatgtaaa ggtttcggc 
      tttgtcacg atgaccaat tatgacgaa gccgtggta 
      gccatccag tcgctaaac ggttacacg ctgggtaac 
      cgtgtcctg caggtcagc tttaaaacg aataaaagt 
      aaaactaca tag
    mRNA sequence
    • >TC034524 RA
      tcggttaat tattgtact ctaggttgg cttaataac 
      cttgcatcg ttagaaaat tattatatt taatcatct 
      ttaaaaata agtgcaatc ctttttaac gcctgtttt 
      aactactga tcactcccg tcattgtac aatactcat 
      acatttctt ccatcatta ccatttctc ttcaatctc 
      gcactgtta gtggatacg taatacagt ttgaatcgt 
      ttaaacgtt ttacaataa tttgtgcta caatcttgg 
      gattcaaca ccaacatca agggattgt agaataggg 
      aaattttct accctttca tacgaagat tgtaataga 
      gttcaaaaa aagttgaag aagtaaacc aagcgcgtt 
      agaaatttc gtattgttt tatactcga ttagtgaat 
      taaacaagt tgattttaa tgtaaaaga agttcttcg 
      caagaagac aaaacagcc gccaccatt gaggaagtt 
      ctagattac gattttgag ttcagcgac gaaaacatg 
      atgtccaac ggattagac tcgggggtt gttggacaa 
      cagaatgga ggcagcggg ggccaagtc ggtgggggt 
      caagaagag tccaaaacg aatctcatc gttaattac 
      ctaccccaa accatgaca caagaagaa atccgatct 
      ttattttcc agtattgga gaagtcgaa agttgcaaa 
      ctaatccgc gataaagtc acagggcaa tcacttggt 
      tatggcttc gtaaactac caccggcca gaagatgca 
      gaaaaagca attaataca ttgaacgga ttaagattg 
      cagaacaag acaattaag gtgtcatat gcacggcca 
      agttccgaa gcaatcaag ggtgcaaac ctgtacgtt 
      tccggatta ccgaagaac atgacccaa caagatttg 
      gaatccctg tttagtccg tacggtcgt ataataaca 
      tcgcgcatt ttatgcgac aacataacc gtacgtcaa 
      tttgtcacg ggggccgga gagaatttg cctggcctg 
      tcgaaagga gtaggtttc atccgtttt gaccaacgt 
      ttagaagca gaacgagca attcaagaa ctcaatggt 
      accattcct aagggctcc accgaacct ataacagtg 
      aaatttgcc aacaacccg agtaacaac aacaaagcc 
      attcctcca ttggcagct tatctaact ccccaagcc 
      actcgccgt ttcgctggc cctatccac cacccaacg 
      ggacgattc aggtattct ccgctggcc ggagacctg 
      ctggccaac tccatgcta cccggaaac gctatgaac 
      ggttccggt tggtgcatt ttcgtgtat aacctcgct 
      ccagagaca gaagagaat gttctctgg cagttgttt 
      ggcccgttc ggagccgtc cagagcgtc aaagtgatc 
      cgtgacctg cagacgaac aaatgtaaa ggtttcggc 
      tttgtcacg atgaccaat tatgacgaa gccgtggta 
      gccatccag tcgctaaac ggttacacg ctgggtaac 
      cgtgtcctg caggtcagc tttaaaacg aataaaagt 
      aaaactaca tagagcgac gtttagtca acaccagcg 
      cacttgccg gacgctgcg cacacgccg ccgatattt 
      aaacattat ttattattt attgttcac tgttcgtgt 
      aaattatac gggctctat gtaggtaat ttaatgcga 
      cggtgtgcg acagcgatc ggcaggaac gggggcgcc 
      cgccaaaag cgcacctcc ctatttaat aacttagta 
      cttataaaa agatacgaa gctagtcag aagtgcatt 
      cgcgtttcc taatagcaa gatttctac ggtctgatg 
      tttttattt aatgtctat aaaaattgt gccatcacc 
      gttaactga catattggg aactcgccc aaagattca 
      taatagcta aacaatata tatttacat gtagtattt 
      attgtataa tttcgtaat ttcgttcaa gatgtaatt 
      ggttagtat aatttatgt atatttata tgtagtgtt 
      ttgtgtatt tcttaaatt gagactagt cgcatcaac 
      aacggacac gcaggctct gaacttgag ttcggtggt 
      gttgtaccg ttcgttgta tttgtaatt tgtgttcgc 
      ggacggccg tggcggcga gggttgact ttaaataaa 
      taaattaca taggaatat atttatata taattgatt 
      agttatgtc tgcatgtga taggtgatg ggcaccaaa 
      ctacatgtt aataaaatt ataggtttt acggtttta 
      ccaccagtt agacatttt atatcggac tatgctgaa 
      aagcgtgac tgactagac gcggacgca gacgcggca 
      tggacgact gaaaatcag tcacaccct caccgcacc 
      ggtgctccg tttattctt gattgtgtg ttgtgttta 
      gtttcacgt ttaccggat ttcaagcca cttacagct 
      ttctgatag gaagaattt gcgtggttt tgctttgaa 
      atgccatca aatgagcga cggcaatgc tttagcgag 
      accacaact ctcccatta atttattat ttccttatg 
      atttatttt cttttcgtt tttgggaac gaaacggac 
      gtttcgtgc cggttttga cttatactt tgttaattt 
      tagtttgat tattttatc aacctggtt cgtttattt 
      atttccaat ataaatcga ttaaaattt gattttgat 
      tttgttttc atttttggt ttcattaca attacattt 
      cctctgttg tgatgtctc acggtgttc gctgacgtc 
      gatgcaact tgctactac gcattgcac aagcggnn
    • >TC034524 RB
      tcggttaat tattgtact ctaggttgg cttaataac 
      cttgcatcg ttagaaaat tattatatt taatcatct 
      ttaaaaata agtgcaatc ctttttaac gcctgtttt 
      aactactga tcactcccg tcattgtac aatactcat 
      acatttctt ccatcatta ccatttctc ttcaatctc 
      gcactgtta gtggatacg taatacagt ttgaatcgt 
      ttaaacgtt ttacaataa tttgtgcta caatcttgg 
      gattcaaca ccaacatca agggattgt agaataggg 
      aaattttct accctttca tacgaagat tgtaataga 
      gttcaaaaa aagttgaag aagtaaacc aagcgcgtt 
      agaaatttc gtattgttt tatactcga ttagtgaat 
      taaacaagt tgattttaa tgtaaaaga agttcttcg 
      caagaagac aaaacagcc gccaccatt gaggaagtt 
      ctagattac gattttgag ttcagcgac gaaaacatg 
      atgtccaac ggattagac tcgggggtt gttggacaa 
      cagaatgga ggcagcggg ggccaagtc ggtgggggt 
      caagaagag tccaaaacg aatctcatc gttaattac 
      ctaccccaa accatgaca caagaagaa atccgatct 
      ttattttcc agtattgga gaagtcgaa agttgcaaa 
      ctaatccgc gataaagtc acagggcaa tcacttggt 
      tatggcttc gtaaactac caccggcca gaagatgca 
      gaaaaagca attaataca ttgaacgga ttaagattg 
      cagaacaag acaattaag gtgtcatat gcacggcca 
      agttccgaa gcaatcaag ggtgcaaac ctgtacgtt 
      tccggatta ccgaagaac atgacccaa caagatttg 
      gaatccctg tttagtccg tacggtcgt ataataaca 
      tcgcgcatt ttatgcgac aacataacc ggcctgtcg 
      aaaggagta ggtttcatc cgttttgac caacgttta 
      gaagcagaa cgagcaatt caagaactc aatggtacc 
      attcctaag ggctccacc gaacctata acagtgaaa 
      tttgccaac aacccgagt aacaacaac aaagccatt 
      cctccattg gcagcttat ctaactccc caagccact 
      cgccgtttc gctggccct atccaccac ccaacggga 
      cgattcagg tattctccg ctggccgga gacctgctg 
      gccaactcc atgctaccc ggaaacgct atgaacggt 
      tccggttgg tgcattttc gtgtataac ctcgctcca 
      gagacagaa gagaatgtt ctctggcag ttgtttggc 
      ccgttcgga gccgtccag agcgtcaaa gtgatccgt 
      gacctgcag acgaacaaa tgtaaaggt ttcggcttt 
      gtcacgatg accaattat gacgaagcc gtggtagcc 
      atccagtcg ctaaacggt tacacgctg ggtaaccgt 
      gtcctgcag gtcagcttt aaaacgaat aaaagtaaa 
      actacatag agcgacgtt tagtcaaca ccagcgcac 
      ttgccggac gctgcgcac acgccgccg atatttaaa 
      cattattta ttatttatt gttcactgt tcgtgtaaa 
      ttatacggg ctctatgta ggtaattta atgcgacgg 
      tgtgcgaca gcgatcggc aggaacggg ggcgcccgc 
      caaaagcgc acctcccta tttaataac ttagtactt 
      ataaaaaga tacgaagct agtcagaag tgcattcgc 
      gtttcctaa tagcaagat ttctacggt ctgatgttt 
      ttatttaat gtctataaa aattgtgcc atcaccgtt 
      aactgacat attgggaac tcgcccaaa gattcataa 
      tagctaaac aatatatat ttacatgta gtatttatt 
      gtataattt cgtaatttc gttcaagat gtaattggt 
      tagtataat ttatgtata tttatatgt agtgttttg 
      tgtatttct taaattgag actagtcgc atcaacaac 
      ggacacgca ggctctgaa cttgagttc ggtggtgtt 
      gtaccgttc gttgtattt gtaatttgt gttcgcgga 
      cggccgtgg cggcgaggg ttgacttta aataaataa 
      attacatag gaatatatt tatatataa ttgattagt 
      tatgtctgc atgtgatag gtgatgggc accaaacta 
      catgttaat aaaattata ggttttacg gttttacca 
      ccagttaga cattttata tcggactat gctgaaaag 
      cgtgactga ctagacgcg gacgcagac gcggcatgg 
      acgactgaa aatcagtca caccctcac cgcaccggt 
      gctccgttt attcttgat tgtgtgttg tgtttagtt 
      tcacgttta ccggatttc aagccactt acagctttc 
      tgataggaa gaatttgcg tggttttgc tttgaaatg 
      ccatcaaat gagcgacgg caatgcttt agcgagacc 
      acaactctc ccattaatt tattatttc cttatgatt 
      tattttctt ttcgttttt gggaacgaa acggacgtt 
      tcgtgccgg ttttgactt atactttgt taattttag 
      tttgattat tttatcaac ctggttcgt ttatttatt 
      tccaatata aatcgatta aaatttgat tttgatttt 
      gttttcatt tttggtttc attacaatt acatttcct 
      ctgttgtga tgtctcacg gtgttcgct gacgtcgat 
      gcaacttgc tactacgca ttgcacaag cggnn
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY