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Gene information
General information
TC014245 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3638606..3642159
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014245
    iBeetle screen iB_06588

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    remarks: eggs lost,
    Adult morphology
    remarks: eggs lost,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: eggs lost,
    remarks - cuticles: later than schedule,
     
    8 days later, due to lost eggs in day 9
     

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014245 RA
      MDRKKSVNP DLIKEREKC TFNTLELTH LLDGSATKT 
      EERHSREKF FLEDPELQS NVPLEYLSH KEKYEDAVR 
      KACIIFRKV QELQNQGKG GMDNFREIL GGQLGSAIL 
      KDGNPLTLH YVMFIPTLM GQGTFDQQA EWIQRAWNC 
      DIIGTYAQT ELGHGTFIR GLETTATYD PATEEFVLH 
      SPTLTSYKW WPGGLGHSA NYAVVVAQL YTKGTCHGI 
      HPFIVQLRD EETHEPLPG IKIGEIGCK LGMNSTNNG 
      YLGFDNVRI PRINMLMKN SQVLPDGTY VKSPSSKLT 
      YGTMIFVRV VIVQDAAHY LRKAVTIAT RYSAVRHQS 
      QIKPDAPEP QIIDFVTQQ YKLFPHIAT YFAFQYSAS 
      WLWDIYNNV TNELESGQL ESLPELHAI ACCLKAVSS 
      ADAAQGVEI CRLACGGHG YMTSSNLPS TYGLVTAMC 
      TYEGENTVL LLQTSRFLI KSWQNRNKL DKLATVAYL 
      GRKMSKSNP FEKSVEWII HALQQTAAS KIELANNRI 
      NARVRNGKS YEDAWNETS IELVAAAEA HCRCFIVTT 
      FYNTVNGLN VSKELKTVL VQLLNLYGV YTALRLTGD 
      LFRFTNITG QDVETLQDW LEELLALIR PNAVGIVDS 
      FDIRDEVLG STLGAYDGN VYERLYEEA SKSPLNSEP 
      VNKSFKSYL KPFMKSNL
    CDS sequence
    • >TC014245 RA
      atggacaga aaaaaatca gtaaatcct gatctcatt 
      aaagagaga gaaaaatgt acattcaat actctagaa 
      ctgacacac ttactggat ggcagtgct acaaaaaca 
      gaggagaga cacagtaga gaaaaattt ttcctcgaa 
      gacccggag ctacaatcg aacgtccca ctggagtat 
      ttaagtcac aaggagaaa tatgaagat gccgtacga 
      aaagcttgt attattttc cgaaaagta caagagctc 
      cagaaccaa ggcaaaggc ggtatggac aatttcaga 
      gaaattttg ggtggtcag ctaggctct gccatttta 
      aaagacggc aatccattg acgctgcac tatgttatg 
      tttatcccg acgctgatg ggacaggga acgtttgac 
      caacaagcc gaatggatc caaagggca tggaattgt 
      gatattata ggaacttac gcacaaact gaattaggt 
      catggcaca tttatcaga ggtcttgaa accacagcc 
      acctacgat cccgcgaca gaggaattt gttttgcac 
      agtccaacc ctcacttcg tacaaatgg tggccggga 
      ggattgggc cattccgcg aattacgca gttgtggtg 
      gcccaactg tacacaaaa ggcacttgc cacggaatc 
      caccctttt atagtccag cttcgtgat gaggaaacg 
      cacgagcct ttaccaggc attaaaatc ggagaaatt 
      ggctgcaaa cttggcatg aacagtacc aacaacggc 
      tacctgggt tttgacaac gtcagaata cccaggatt 
      aacatgtta atgaaaaat agtcaagtt ttacctgat 
      ggcacttac gttaaatcg cccagtagt aagctaact 
      tacggaaca atgattttc gtccgagtt gtgatagta 
      caggatgcc gcacactac ttgcgaaag gctgtaacc 
      atagcaaca cgctacagc gccgttcgc caccaatca 
      caaatcaaa cccgatgca cccgagccc caaataatc 
      gatttcgtg acgcaacag tataagtta ttcccgcac 
      atcgccact tattttgca ttccagtat agtgcctct 
      tggctgtgg gacatttac aacaatgtc accaacgag 
      ctggagtcc ggtcagctc gagagtttg cctgaactc 
      cacgctatt gcctgttgc cttaaagct gtgagcagt 
      gccgatgca gcccagggt gtggaaatt tgccggttg 
      gcctgtggg ggccacggc tacatgacc tcgtccaac 
      ctgccctca acttacggc ctagtgacg gcaatgtgc 
      acttacgag ggcgaaaac accgtactt ctgttgcaa 
      acatcacgt tttcttata aagtcgtgg cagaatcgt 
      aacaaattg gataagttg gcgactgtg gcgtacttg 
      ggccgcaaa atgagcaaa agtaacccg tttgaaaag 
      tcagttgag tggattata cacgcactg cagcaaact 
      gcggcaagt aaaattgaa ctggcaaat aaccgtata 
      aatgcccgg gtgcgcaat ggaaagtcg tacgaagat 
      gcctggaat gaaacttcg attgagttg gtggcggct 
      gccgaagct cactgtcgg tgttttatt gttacaacg 
      ttttacaac actgttaac ggtttgaac gtctctaaa 
      gagctcaaa actgttctg gtacaactt ttgaacttg 
      tatggcgtt tatacggcc cttagatta acaggggat 
      ctttttaga ttcacgaat atcaccgga caagatgtg 
      gagacactc caggactgg ctggaagaa ctcttggcc 
      cttatacgc cccaatgca gtgggaata gttgatagt 
      tttgacatt cgtgacgaa gttctaggg tctaccttg 
      ggagcttat gacggaaac gtgtacgaa agactgtat 
      gaagaagca tccaaaagt ccactcaat tccgagcca 
      gttaacaag tcattcaaa tcgtactta aaacctttc 
      atgaaatcg aacttgtaa
    mRNA sequence
    • >TC014245 RA
      tctggttaa gaagtctga ggtgacgag tttgttggg 
      tgaaatatc atgcgccct ctgtaggca ggagtggca 
      gtggtggca atagattta tgattaatt accgtgtgt 
      agtgatgtg gtagtactg tcatagagt gtcggtgat 
      agtggttca aagtataat cttttgttt attttgtgt 
      atttaacga aaatggaca gaaaaaaat cagtaaatc 
      ctgatctca ttaaagaga gagaaaaat gtacattca 
      atactctag aactgacac acttactgg atggcagtg 
      ctacaaaaa cagaggaga gacacagta gagaaaaat 
      ttttcctcg aagacccgg agctacaat cgaacgtcc 
      cactggagt atttaagtc acaaggaga aatatgaag 
      atgccgtac gaaaagctt gtattattt tccgaaaag 
      tacaagagc tccagaacc aaggcaaag gcggtatgg 
      acaatttca gagaaattt tgggtggtc agctaggct 
      ctgccattt taaaagacg gcaatccat tgacgctgc 
      actatgtta tgtttatcc cgacgctga tgggacagg 
      gaacgtttg accaacaag ccgaatgga tccaaaggg 
      catggaatt gtgatatta taggaactt acgcacaaa 
      ctgaattag gtcatggca catttatca gaggtcttg 
      aaaccacag ccacctacg atcccgcga cagaggaat 
      ttgttttgc acagtccaa ccctcactt cgtacaaat 
      ggtggccgg gaggattgg gccattccg cgaattacg 
      cagttgtgg tggcccaac tgtacacaa aaggcactt 
      gccacggaa tccaccctt ttatagtcc agcttcgtg 
      atgaggaaa cgcacgagc ctttaccag gcattaaaa 
      tcggagaaa ttggctgca aacttggca tgaacagta 
      ccaacaacg gctacctgg gttttgaca acgtcagaa 
      tacccagga ttaacatgt taatgaaaa atagtcaag 
      ttttacctg atggcactt acgttaaat cgcccagta 
      gtaagctaa cttacggaa caatgattt tcgtccgag 
      ttgtgatag tacaggatg ccgcacact acttgcgaa 
      aggctgtaa ccatagcaa cacgctaca gcgccgttc 
      gccaccaat cacaaatca aacccgatg cacccgagc 
      cccaaataa tcgatttcg tgacgcaac agtataagt 
      tattcccgc acatcgcca cttattttg cattccagt 
      atagtgcct cttggctgt gggacattt acaacaatg 
      tcaccaacg agctggagt ccggtcagc tcgagagtt 
      tgcctgaac tccacgcta ttgcctgtt gccttaaag 
      ctgtgagca gtgccgatg cagcccagg gtgtggaaa 
      tttgccggt tggcctgtg ggggccacg gctacatga 
      cctcgtcca acctgccct caacttacg gcctagtga 
      cggcaatgt gcacttacg agggcgaaa acaccgtac 
      ttctgttgc aaacatcac gttttctta taaagtcgt 
      ggcagaatc gtaacaaat tggataagt tggcgactg 
      tggcgtact tgggccgca aaatgagca aaagtaacc 
      cgtttgaaa agtcagttg agtggatta tacacgcac 
      tgcagcaaa ctgcggcaa gtaaaattg aactggcaa 
      ataaccgta taaatgccc gggtgcgca atggaaagt 
      cgtacgaag atgcctgga atgaaactt cgattgagt 
      tggtggcgg ctgccgaag ctcactgtc ggtgtttta 
      ttgttacaa cgttttaca acactgtta acggtttga 
      acgtctcta aagagctca aaactgttc tggtacaac 
      ttttgaact tgtatggcg tttatacgg cccttagat 
      taacagggg atcttttta gattcacga atatcaccg 
      gacaagatg tggagacac tccaggact ggctggaag 
      aactcttgg cccttatac gccccaatg cagtgggaa 
      tagttgata gttttgaca ttcgtgacg aagttctag 
      ggtctacct tgggagctt atgacggaa acgtgtacg 
      aaagactgt atgaagaag catccaaaa gtccactca 
      attccgagc cagttaaca agtcattca aatcgtact 
      taaaacctt tcatgaaat cgaacttgt aaattttga 
      aaaaatatt taggatagc tttggaata ggggaccag 
      cggtagtta attgtatga acaaagtca aagttgtga 
      ttggtgttt gtcttgtaa aaatgttga gagtcatta 
      tgtttgtta ataaaaaat taaacattg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY