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Gene information
General information
TC014244 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3630826..3636161
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014244
    iBeetle screen iB_06587

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    remarks: eggs lost,
    Adult morphology
    remarks: eggs lost,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: eggs lost,
    remarks - cuticles: later than schedule,
     
    8 days later, due to lost eggs in day 9
     

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014244 RA
      MSKHEIPVS PIQVYVRQR PLTACEIKA KRHSIIKGM 
      NQNELFLEY ASFAKKYSF DRVFGGGAS QEEVYDVVV 
      RPLVDSMLL GNTCSLFAF GPSKTGKSY TQLGPHLSP 
      TVDLVNDPF VGMIPRAAV DILDKLSQL GVECVVTAS 
      FVELYNEEI RDLVVNDNS GLSVHDDPN YKGVTRVKG 
      VTEIRVKNC TELFEMLMV GANNRSEPS LHCHTIFTI 
      GVTIFESFK GEKIVKTGR IHLIDLAGS NFLASCGAL 
      VINKALQTF KTVIQALAS ESKHVPMRD SKLTRVLQD 
      CLNGKGNTS IIATISPCS DAFEDTVTT LDYVQMARG 
      VSTNPVPNV INIRRDDEV KNLEDEVSS LRRDLGTAG 
      SFRAEIENY AKLMELKVN HEKAVALTA EITKLEGVC 
      EHQKYYPDT GELAQETTQ KCEPQAEAK NAEIATEMV 
      HVSFEALSG VEFAVELFQ NDERSNLSD IATNNEKVR 
      EVTFMNLGR LRTILSNVC AFADSLKCT LDSLLSASE 
      FTETSARAN LTTMHIENH SILADNYHR VKATLCKLQ 
      GYIESTKTS LAEATFQYE DDVGMQYSL LSDMYERFY 
      TLLTNLHAT MQKRYLRIQ HLMSLSNQR LLLLDKITK 
      NFLNTQEDL TLELETMCY IRGYAGQLT ELLQEKAPG 
      IDNKLITLF TKFNQETAH LHLISLQRR FEAAKEEIQ 
      QSQTVCLEE VAQNHSRHQ DYFRSFFDA IRRGVATHN 
      EGVVATRDG LSFEAYMMV SHQYNMTVD RTSQISNSL 
      DDMFSNAKD LTSEMEDIS TETVAKLKQ LSEKVTVNS 
      PKEKKEVKK TLLQEKQDN SNEPPNENQ
    CDS sequence
    • >TC014244 RA
      atgagtaaa cacgagata ccggtatca cctatccag 
      gtgtacgtc aggcagaga cctctcacg gcgtgcgaa 
      attaaagcc aaaaggcat agcattatt aaaggcatg 
      aatcagaac gagctcttt cttgaatac gcgtctttt 
      gcgaaaaag tacagtttt gatcgagtt tttgggggt 
      ggtgcatcg caagaggag gtttacgat gtcgtggtg 
      cgccccctg gttgactca atgttgtta gggaacact 
      tgcagtctt ttcgcgttt ggaccaagt aaaaccggc 
      aaatcctac actcagctg gggccccac ttgagcccc 
      acggttgat ctcgttaac gacccgttt gttgggatg 
      atcccaagg gctgctgtt gatattctc gataagctg 
      tctcagtta ggggttgag tgtgtcgtt actgcatcc 
      tttgttgag ttatacaat gaagaaatc cgtgacctt 
      gtggttaat gacaattct ggcttgagc gtccatgat 
      gatccgaat tacaagggg gtgacacgt gttaagggt 
      gtgacggag attcgagta aaaaattgc actgaactg 
      tttgaaatg ttaatggtt ggagcgaat aatcgtagc 
      gagccaagt ttacattgt cacacaatt ttcaccatt 
      ggtgttacg attttcgaa tcatttaag ggtgaaaag 
      atagttaaa actggccga attcacttg attgattta 
      gctggaagt aatttcctt gcatcgtgt ggtgcgtta 
      gtcattaat aaggcgctt caaactttc aaaacagtg 
      attcaagcc ctcgcttct gaatctaaa catgttccc 
      atgagagac tcaaaactg acccgagtt ttgcaagac 
      tgcctcaac ggcaaaggc aatacttcg attattgca 
      acaatttca ccctgtagt gacgcattt gaagacaca 
      gtcactact ttggactac gtgcaaatg gcgcgaggc 
      gtctccact aacccggtc cccaacgta ataaacata 
      cgcagagat gatgaagtg aagaattta gaagacgaa 
      gttagttct ctacgccgg gatttaggc acagccggg 
      tctttcaga gcggagatc gaaaactac gcaaagttg 
      atggaactt aaagtaaac catgaaaaa gccgtcgca 
      cttacggcg gaaataaca aaattagag ggcgtatgt 
      gagcaccaa aagtattat cctgatacc ggagagttg 
      gcgcaagaa acaacccaa aaatgtgaa ccccaggcc 
      gaggcaaag aatgcggaa attgccaca gaaatggtc 
      cacgtttct ttcgaagcc ttgagcggg gttgagttc 
      gctgtcgaa ctcttccaa aacgatgag agatcgaat 
      ttgtctgat atagccaca aataatgag aaggttaga 
      gaggtcaca tttatgaat ttgggacgc ttgaggact 
      atcctaagc aatgtttgc gcatttgcc gattcgcta 
      aaatgcacg ttggactct ttgttgagc gcgtcggag 
      ttcaccgaa actagcgcc agggcgaat ttaacgacc 
      atgcatatc gagaatcat tcaatcttg gctgataat 
      tatcacaga gtgaaagca actctgtgc aaacttcaa 
      ggttatata gaatctacc aaaacatca ttggcggag 
      gcaacgttt cagtatgag gacgatgtc gggatgcag 
      tactcctta ctgagcgac atgtacgag agattctac 
      acgctactg actaattta catgcaacc atgcaaaaa 
      cgttacttg cgaattcaa catttaatg agcctcagt 
      aatcaaaga ctgctattg ttggacaag ataactaag 
      aattttctt aacacccaa gaggatttg actttggaa 
      ttggagacc atgtgctac ataaggggg tatgctggt 
      caactgacc gaattgttg caggaaaaa gctccagga 
      atcgacaat aagttaatt acgcttttt actaagttc 
      aatcaggag actgcacat cttcatttg atttcgctt 
      cagagaaga tttgaggcg gctaaagaa gaaattcag 
      caaagccaa acagtttgc ttggaggaa gttgctcaa 
      aatcacagc cgacatcaa gactacttt cgtagtttc 
      tttgatgct attagaaga ggtgtagcg actcataat 
      gagggagtt gttgcgacc agggatggt ttgtcattt 
      gaagcttac atgatggta agccatcaa tataatatg 
      actgtggac cgcacctca cagatctca aactcactt 
      gatgacatg ttctcgaac gcaaaggat ttaacttcg 
      gagatggaa gatatttct actgaaacc gttgctaaa 
      ctcaaacag ttgtcagaa aaagttacg gttaattct 
      ccgaaagaa aagaaagaa gttaagaag actctcctg 
      caggagaag caagataat tcaaacgag ccaccgaat 
      gaaaatcag taa
    mRNA sequence
    • >TC014244 RA
      ttcgtcgtc aaaaaaaaa acacccgcc acacataac 
      ggtttttgt ttttgtaat ttcatcccc tcaggttgg 
      tatgcattt ttccgtccg acttgaaac ttctcagag 
      cgtccttgt tgtttgcca gttgtttgt ggttgtttt 
      ttaggaaaa aaaccacaa atagtggtg aaaaagtgt 
      gcaaaatga gtaaacacg agataccgg tatcaccta 
      tccaggtgt acgtcaggc agagacctc tcacggcgt 
      gcgaaatta aagccaaaa ggcatagca ttattaaag 
      gcatgaatc agaacgagc tctttcttg aatacgcgt 
      cttttgcga aaaagtaca gttttgatc gagtttttg 
      ggggtggtg catcgcaag aggaggttt acgatgtcg 
      tggtgcgcc ccctggttg actcaatgt tgttaggga 
      acacttgca gtcttttcg cgtttggac caagtaaaa 
      ccggcaaat cctacactc agctggggc cccacttga 
      gccccacgg ttgatctcg ttaacgacc cgtttgttg 
      ggatgatcc caagggctg ctgttgata ttctcgata 
      agctgtctc agttagggg ttgagtgtg tcgttactg 
      catcctttg ttgagttat acaatgaag aaatccgtg 
      accttgtgg ttaatgaca attctggct tgagcgtcc 
      atgatgatc cgaattaca agggggtga cacgtgtta 
      agggtgtga cggagattc gagtaaaaa attgcactg 
      aactgtttg aaatgttaa tggttggag cgaataatc 
      gtagcgagc caagtttac attgtcaca caattttca 
      ccattggtg ttacgattt tcgaatcat ttaagggtg 
      aaaagatag ttaaaactg gccgaattc acttgattg 
      atttagctg gaagtaatt tccttgcat cgtgtggtg 
      cgttagtca ttaataagg cgcttcaaa ctttcaaaa 
      cagtgattc aagccctcg cttctgaat ctaaacatg 
      ttcccatga gagactcaa aactgaccc gagttttgc 
      aagactgcc tcaacggca aaggcaata cttcgatta 
      ttgcaacaa tttcaccct gtagtgacg catttgaag 
      acacagtca ctactttgg actacgtgc aaatggcgc 
      gaggcgtct ccactaacc cggtcccca acgtaataa 
      acatacgca gagatgatg aagtgaaga atttagaag 
      acgaagtta gttctctac gccgggatt taggcacag 
      ccgggtctt tcagagcgg agatcgaaa actacgcaa 
      agttgatgg aacttaaag taaaccatg aaaaagccg 
      tcgcactta cggcggaaa taacaaaat tagagggcg 
      tatgtgagc accaaaagt attatcctg ataccggag 
      agttggcgc aagaaacaa cccaaaaat gtgaacccc 
      aggccgagg caaagaatg cggaaattg ccacagaaa 
      tggtccacg tttctttcg aagccttga gcggggttg 
      agttcgctg tcgaactct tccaaaacg atgagagat 
      cgaatttgt ctgatatag ccacaaata atgagaagg 
      ttagagagg tcacattta tgaatttgg gacgcttga 
      ggactatcc taagcaatg tttgcgcat ttgccgatt 
      cgctaaaat gcacgttgg actctttgt tgagcgcgt 
      cggagttca ccgaaacta gcgccaggg cgaatttaa 
      cgaccatgc atatcgaga atcattcaa tcttggctg 
      ataattatc acagagtga aagcaactc tgtgcaaac 
      ttcaaggtt atatagaat ctaccaaaa catcattgg 
      cggaggcaa cgtttcagt atgaggacg atgtcggga 
      tgcagtact ccttactga gcgacatgt acgagagat 
      tctacacgc tactgacta atttacatg caaccatgc 
      aaaaacgtt acttgcgaa ttcaacatt taatgagcc 
      tcagtaatc aaagactgc tattgttgg acaagataa 
      ctaagaatt ttcttaaca cccaagagg atttgactt 
      tggaattgg agaccatgt gctacataa gggggtatg 
      ctggtcaac tgaccgaat tgttgcagg aaaaagctc 
      caggaatcg acaataagt taattacgc tttttacta 
      agttcaatc aggagactg cacatcttc atttgattt 
      cgcttcaga gaagatttg aggcggcta aagaagaaa 
      ttcagcaaa gccaaacag tttgcttgg aggaagttg 
      ctcaaaatc acagccgac atcaagact actttcgta 
      gtttctttg atgctatta gaagaggtg tagcgactc 
      ataatgagg gagttgttg cgaccaggg atggtttgt 
      catttgaag cttacatga tggtaagcc atcaatata 
      atatgactg tggaccgca cctcacaga tctcaaact 
      cacttgatg acatgttct cgaacgcaa aggatttaa 
      cttcggaga tggaagata tttctactg aaaccgttg 
      ctaaactca aacagttgt cagaaaaag ttacggtta 
      attctccga aagaaaaga aagaagtta agaagactc 
      tcctgcagg agaagcaag ataattcaa acgagccac 
      cgaatgaaa atcagtaat ccgtactga tttaatcgt 
      tctgctagt tttaggttg ttacgttac ttttttttt 
      atttatttg taatgaaac aagttttgc tgtttgtgt 
      ttgcaaaac ttgctagtc gaattggtg aagttataa 
      ctctcacca atagtactc aaagagtat tagaagttc 
      ttaccggag aactttcgc cttttgata tttatgtgc 
      aatgaaacg attttgcta agactagat acatttcag 
      tcaaggtaa aaaagtgat ggtagtttc gctgattgg 
      tctaaagct ttaaacgat acctagtca aatgtttca 
      ctgtgcaaa ccttagtat tatattgtt tttggtgtt 
      aatgttcct tcttgcaat gttccgtat gttttttta 
      aaacatacc aattaaacc aaagttgaa aatgtgtta 
      aatatagct ttgccgctt gtttcgcaa gctataaat 
      cctaactag tctaactag aagttgaaa atgttacca 
      ttatatttg aatttctta atattatat tgttcatga 
      tgttctgtt ccatgtttg taatgttct gattgtgtt 
      tggacttac aaataaact taaaaaaaa cat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY