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Gene information
General information
TC013566 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3608172..3628182
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013566
    iBeetle screen iB_06586

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    remarks: eggs lost,
    Adult morphology
    remarks: eggs lost,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: eggs lost,
    remarks - cuticles: later than schedule,
     
    8 days later, due to lost eggs in day 9
     

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013566 RA
      MFTRFDANK STKGASKLR RDLINAEIA NLRELLPLP 
      PSTRQRLSQ LQLMALVCV YVRKANYFQ QAFKCHDVG 
      LHQSPTPNI GFSKALNGF LMMMTQNGK LLYISDNAA 
      EYLGHSMED LLIHGDSVY DIIDKQDHQ AIQCELGRP 
      VNSGSNSST SEEIRLFLC RMNVSRNAR RQMRFGDQK 
      VVLVQGRYL SFLPLCSRN EPVFLATCT PVAMPETRE 
      CVVQGATNV FTSVHSMDM RFIHIDNNG EFYLGFPRA 
      DLQGVSWYR LLHWECTRE AQTKHRLIT QSEQERSCI 
      LLAQFQRRS GDWIWMHCV LQVKESSES SQQPVIVCT 
      NQVLSEAEA AVMRTNNWL YHYYSVQNK LQYGLAYDI 
      GPPSRLLYY PENPPGQEF TGLVARPVK
    • >TC013566 RB
      MFTRFDANK STKGASKLR RDLINAEIA NLRELLPLP 
      PSTRQRLSQ LQLMALVCV YVRKANYFQ QAFKCHDVG 
      LHQSPTPNI GFSKALNGF LMMMTQNGK LLYISDNAA 
      EYLGHSMVS VLQEDLLIH GDSVYDIID KQDHQAIQC 
      ELGRPVNSG SNSSTSEEI RLFLCRMNV SRNARRQMR 
      FGDQKVVLV QGRYLSFLP LCSRNEPVF LATCTPVAM 
      PETRECVVQ GATNVFTSV HSMDMRFIH IDNNGEFYL 
      GFPRADLQG VSWYRLLHW ECTREAQTK HRLITQSEQ 
      ERSCILLAQ FQRRSGDWI WMHCVLQVK ESSESSQQP 
      VIVCTNQVL SEAEAAVMR TNNWLYHYY SVQNKLQYG 
      LAYDIGPPS RLLYYPENP PGQEFTGLV ARPVK
    CDS sequence
    • >TC013566 RA
      atgttcaca aggtttgat gcaaacaaa tcgaccaaa 
      ggagccagc aagctacga agagaccta ataaatgca 
      gaaatagca aatttacgt gagttatta cctttacca 
      ccatcgacg aggcaaagg ctttcacaa ctgcagctt 
      atggcacta gtctgtgta tatgtcagg aaagctaat 
      tattttcaa caagcattc aaatgccat gatgtggga 
      ttgcaccag tcaccaaca cccaacatc ggattttca 
      aaggcattg aacggcttt ctgatgatg atgactcaa 
      aatggtaag ctgttgtat atatcggac aatgctgcg 
      gagtacctg ggacattcc atggaggac ctgttgatc 
      catggagat agtgtatac gatattata gataagcag 
      gatcaccaa gcaattcaa tgcgaattg ggacggcca 
      gtaaattca ggatcaaat tctagcact agtgaagaa 
      attcgttta tttttatgt aggatgaat gtttctaga 
      aacgcaagg cggcaaatg aggttcggg gaccaaaag 
      gtagtgctg gtgcagggt cgatacttg tcgtttttg 
      ccattatgc agtcgaaac gaaccagtc ttcctggcc 
      acctgtacg ccagtggcg atgccagaa accagagag 
      tgtgtcgtg cagggcgcc accaacgtc ttcacttca 
      gtacactcg atggatatg cgatttatc cacatcgac 
      aacaacggc gaattctac ttgggtttt ccgcgcgcc 
      gatctgcag ggggtgtcc tggtaccgg ctgctgcat 
      tgggaatgc acgcgtgag gcccagaca aaacaccga 
      ctgatcacc caatcggaa caggagcgg tcgtgtatt 
      ctgctagcg cagtttcag cgacgctcc ggtgactgg 
      atttggatg cactgcgtc ctgcaagtc aaagaaagc 
      tccgaaagc agtcagcag cccgtcatc gtctgcacc 
      aaccaagtg ctgagcgag gccgaagcg gccgtcatg 
      cggaccaac aactggctc tatcactat tattccgtg 
      caaaacaaa ctccagtat ggtctcgcc tatgacatt 
      ggaccaccc tcacggctc ctctactac ccggaaaac 
      ccacccggg caggaattc accgggtta gtggccagg 
      ccagtaaaa taa
    • >TC013566 RB
      atgttcaca aggtttgat gcaaacaaa tcgaccaaa 
      ggagccagc aagctacga agagaccta ataaatgca 
      gaaatagca aatttacgt gagttatta cctttacca 
      ccatcgacg aggcaaagg ctttcacaa ctgcagctt 
      atggcacta gtctgtgta tatgtcagg aaagctaat 
      tattttcaa caagcattc aaatgccat gatgtggga 
      ttgcaccag tcaccaaca cccaacatc ggattttca 
      aaggcattg aacggcttt ctgatgatg atgactcaa 
      aatggtaag ctgttgtat atatcggac aatgctgcg 
      gagtacctg ggacattcc atggtgagt gttttacag 
      gaggacctg ttgatccat ggagatagt gtatacgat 
      attatagat aagcaggat caccaagca attcaatgc 
      gaattggga cggccagta aattcagga tcaaattct 
      agcactagt gaagaaatt cgtttattt ttatgtagg 
      atgaatgtt tctagaaac gcaaggcgg caaatgagg 
      ttcggggac caaaaggta gtgctggtg cagggtcga 
      tacttgtcg tttttgcca ttatgcagt cgaaacgaa 
      ccagtcttc ctggccacc tgtacgcca gtggcgatg 
      ccagaaacc agagagtgt gtcgtgcag ggcgccacc 
      aacgtcttc acttcagta cactcgatg gatatgcga 
      tttatccac atcgacaac aacggcgaa ttctacttg 
      ggttttccg cgcgccgat ctgcagggg gtgtcctgg 
      taccggctg ctgcattgg gaatgcacg cgtgaggcc 
      cagacaaaa caccgactg atcacccaa tcggaacag 
      gagcggtcg tgtattctg ctagcgcag tttcagcga 
      cgctccggt gactggatt tggatgcac tgcgtcctg 
      caagtcaaa gaaagctcc gaaagcagt cagcagccc 
      gtcatcgtc tgcaccaac caagtgctg agcgaggcc 
      gaagcggcc gtcatgcgg accaacaac tggctctat 
      cactattat tccgtgcaa aacaaactc cagtatggt 
      ctcgcctat gacattgga ccaccctca cggctcctc 
      tactacccg gaaaaccca cccgggcag gaattcacc 
      gggttagtg gccaggcca gtaaaataa
    mRNA sequence
    • >TC013566 RA
      actacgcga tcgtatttt tattctaaa actcacgtg 
      ctttgtcca ttcgcgccg tttttcgta ctacaatgt 
      gtgtgtgaa tgtgttatt tgtattttc attaactga 
      caaacatgt tcacaaggt ttgatgcaa acaaatcga 
      ccaaaggag ccagcaagc tacgaagag acctaataa 
      atgcagaaa tagcaaatt tacgtgagt tattacctt 
      taccaccat cgacgaggc aaaggcttt cacaactgc 
      agcttatgg cactagtct gtgtatatg tcaggaaag 
      ctaattatt ttcaacaag cattcaaat gccatgatg 
      tgggattgc accagtcac caacaccca acatcggat 
      tttcaaagg cattgaacg gctttctga tgatgatga 
      ctcaaaatg gtaagctgt tgtatatat cggacaatg 
      ctgcggagt acctgggac attccatgg aggacctgt 
      tgatccatg gagatagtg tatacgata ttatagata 
      agcaggatc accaagcaa ttcaatgcg aattgggac 
      ggccagtaa attcaggat caaattcta gcactagtg 
      aagaaattc gtttatttt tatgtagga tgaatgttt 
      ctagaaacg caaggcggc aaatgaggt tcggggacc 
      aaaaggtag tgctggtgc agggtcgat acttgtcgt 
      ttttgccat tatgcagtc gaaacgaac cagtcttcc 
      tggccacct gtacgccag tggcgatgc cagaaacca 
      gagagtgtg tcgtgcagg gcgccacca acgtcttca 
      cttcagtac actcgatgg atatgcgat ttatccaca 
      tcgacaaca acggcgaat tctacttgg gttttccgc 
      gcgccgatc tgcaggggg tgtcctggt accggctgc 
      tgcattggg aatgcacgc gtgaggccc agacaaaac 
      accgactga tcacccaat cggaacagg agcggtcgt 
      gtattctgc tagcgcagt ttcagcgac gctccggtg 
      actggattt ggatgcact gcgtcctgc aagtcaaag 
      aaagctccg aaagcagtc agcagcccg tcatcgtct 
      gcaccaacc aagtgctga gcgaggccg aagcggccg 
      tcatgcgga ccaacaact ggctctatc actattatt 
      ccgtgcaaa acaaactcc agtatggtc tcgcctatg 
      acattggac caccctcac ggctcctct actacccgg 
      aaaacccac ccgggcagg aattcaccg ggttagtgg 
      ccaggccag taaaataac actttcatt tgaaaacgt 
      ctagtccac gaatttttt cttttaacc accgacgaa 
      aataaagtg tttttgata ct
    • >TC013566 RB
      actacgcga tcgtatttt tattctaaa actcacgtg 
      ctttgtcca ttcgcgccg tttttcgta ctacaatgt 
      gtgtgtgaa tgtgttatt tgtattttc attaactga 
      caaacatgt tcacaaggt ttgatgcaa acaaatcga 
      ccaaaggag ccagcaagc tacgaagag acctaataa 
      atgcagaaa tagcaaatt tacgtgagt tattacctt 
      taccaccat cgacgaggc aaaggcttt cacaactgc 
      agcttatgg cactagtct gtgtatatg tcaggaaag 
      ctaattatt ttcaacaag cattcaaat gccatgatg 
      tgggattgc accagtcac caacaccca acatcggat 
      tttcaaagg cattgaacg gctttctga tgatgatga 
      ctcaaaatg gtaagctgt tgtatatat cggacaatg 
      ctgcggagt acctgggac attccatgg tgagtgttt 
      tacaggagg acctgttga tccatggag atagtgtat 
      acgatatta tagataagc aggatcacc aagcaattc 
      aatgcgaat tgggacggc cagtaaatt caggatcaa 
      attctagca ctagtgaag aaattcgtt tatttttat 
      gtaggatga atgtttcta gaaacgcaa ggcggcaaa 
      tgaggttcg gggaccaaa aggtagtgc tggtgcagg 
      gtcgatact tgtcgtttt tgccattat gcagtcgaa 
      acgaaccag tcttcctgg ccacctgta cgccagtgg 
      cgatgccag aaaccagag agtgtgtcg tgcagggcg 
      ccaccaacg tcttcactt cagtacact cgatggata 
      tgcgattta tccacatcg acaacaacg gcgaattct 
      acttgggtt ttccgcgcg ccgatctgc agggggtgt 
      cctggtacc ggctgctgc attgggaat gcacgcgtg 
      aggcccaga caaaacacc gactgatca cccaatcgg 
      aacaggagc ggtcgtgta ttctgctag cgcagtttc 
      agcgacgct ccggtgact ggatttgga tgcactgcg 
      tcctgcaag tcaaagaaa gctccgaaa gcagtcagc 
      agcccgtca tcgtctgca ccaaccaag tgctgagcg 
      aggccgaag cggccgtca tgcggacca acaactggc 
      tctatcact attattccg tgcaaaaca aactccagt 
      atggtctcg cctatgaca ttggaccac cctcacggc 
      tcctctact acccggaaa acccacccg ggcaggaat 
      tcaccgggt tagtggcca ggccagtaa aataacact 
      ttcatttga aaacgtcta gtccacgaa ttttttctt 
      ttaaccacc gacgaaaat aaagtgttt ttgatact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY