| General information | |||
| TC013566 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 3608172..3628182 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013566 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06586 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
remarks: eggs lost,
remarks: eggs lost,
remarks - cuticles: later than schedule,
|
8 days later, due to lost eggs in day 9
|
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC013566 RA
MFTRFDANK STKGASKLR RDLINAEIA NLRELLPLP PSTRQRLSQ LQLMALVCV YVRKANYFQ QAFKCHDVG LHQSPTPNI GFSKALNGF LMMMTQNGK LLYISDNAA EYLGHSMED LLIHGDSVY DIIDKQDHQ AIQCELGRP VNSGSNSST SEEIRLFLC RMNVSRNAR RQMRFGDQK VVLVQGRYL SFLPLCSRN EPVFLATCT PVAMPETRE CVVQGATNV FTSVHSMDM RFIHIDNNG EFYLGFPRA DLQGVSWYR LLHWECTRE AQTKHRLIT QSEQERSCI LLAQFQRRS GDWIWMHCV LQVKESSES SQQPVIVCT NQVLSEAEA AVMRTNNWL YHYYSVQNK LQYGLAYDI GPPSRLLYY PENPPGQEF TGLVARPVK
- >TC013566 RB
MFTRFDANK STKGASKLR RDLINAEIA NLRELLPLP PSTRQRLSQ LQLMALVCV YVRKANYFQ QAFKCHDVG LHQSPTPNI GFSKALNGF LMMMTQNGK LLYISDNAA EYLGHSMVS VLQEDLLIH GDSVYDIID KQDHQAIQC ELGRPVNSG SNSSTSEEI RLFLCRMNV SRNARRQMR FGDQKVVLV QGRYLSFLP LCSRNEPVF LATCTPVAM PETRECVVQ GATNVFTSV HSMDMRFIH IDNNGEFYL GFPRADLQG VSWYRLLHW ECTREAQTK HRLITQSEQ ERSCILLAQ FQRRSGDWI WMHCVLQVK ESSESSQQP VIVCTNQVL SEAEAAVMR TNNWLYHYY SVQNKLQYG LAYDIGPPS RLLYYPENP PGQEFTGLV ARPVK
- >TC013566 RA
atgttcaca aggtttgat gcaaacaaa tcgaccaaa ggagccagc aagctacga agagaccta ataaatgca gaaatagca aatttacgt gagttatta cctttacca ccatcgacg aggcaaagg ctttcacaa ctgcagctt atggcacta gtctgtgta tatgtcagg aaagctaat tattttcaa caagcattc aaatgccat gatgtggga ttgcaccag tcaccaaca cccaacatc ggattttca aaggcattg aacggcttt ctgatgatg atgactcaa aatggtaag ctgttgtat atatcggac aatgctgcg gagtacctg ggacattcc atggaggac ctgttgatc catggagat agtgtatac gatattata gataagcag gatcaccaa gcaattcaa tgcgaattg ggacggcca gtaaattca ggatcaaat tctagcact agtgaagaa attcgttta tttttatgt aggatgaat gtttctaga aacgcaagg cggcaaatg aggttcggg gaccaaaag gtagtgctg gtgcagggt cgatacttg tcgtttttg ccattatgc agtcgaaac gaaccagtc ttcctggcc acctgtacg ccagtggcg atgccagaa accagagag tgtgtcgtg cagggcgcc accaacgtc ttcacttca gtacactcg atggatatg cgatttatc cacatcgac aacaacggc gaattctac ttgggtttt ccgcgcgcc gatctgcag ggggtgtcc tggtaccgg ctgctgcat tgggaatgc acgcgtgag gcccagaca aaacaccga ctgatcacc caatcggaa caggagcgg tcgtgtatt ctgctagcg cagtttcag cgacgctcc ggtgactgg atttggatg cactgcgtc ctgcaagtc aaagaaagc tccgaaagc agtcagcag cccgtcatc gtctgcacc aaccaagtg ctgagcgag gccgaagcg gccgtcatg cggaccaac aactggctc tatcactat tattccgtg caaaacaaa ctccagtat ggtctcgcc tatgacatt ggaccaccc tcacggctc ctctactac ccggaaaac ccacccggg caggaattc accgggtta gtggccagg ccagtaaaa taa
- >TC013566 RB
atgttcaca aggtttgat gcaaacaaa tcgaccaaa ggagccagc aagctacga agagaccta ataaatgca gaaatagca aatttacgt gagttatta cctttacca ccatcgacg aggcaaagg ctttcacaa ctgcagctt atggcacta gtctgtgta tatgtcagg aaagctaat tattttcaa caagcattc aaatgccat gatgtggga ttgcaccag tcaccaaca cccaacatc ggattttca aaggcattg aacggcttt ctgatgatg atgactcaa aatggtaag ctgttgtat atatcggac aatgctgcg gagtacctg ggacattcc atggtgagt gttttacag gaggacctg ttgatccat ggagatagt gtatacgat attatagat aagcaggat caccaagca attcaatgc gaattggga cggccagta aattcagga tcaaattct agcactagt gaagaaatt cgtttattt ttatgtagg atgaatgtt tctagaaac gcaaggcgg caaatgagg ttcggggac caaaaggta gtgctggtg cagggtcga tacttgtcg tttttgcca ttatgcagt cgaaacgaa ccagtcttc ctggccacc tgtacgcca gtggcgatg ccagaaacc agagagtgt gtcgtgcag ggcgccacc aacgtcttc acttcagta cactcgatg gatatgcga tttatccac atcgacaac aacggcgaa ttctacttg ggttttccg cgcgccgat ctgcagggg gtgtcctgg taccggctg ctgcattgg gaatgcacg cgtgaggcc cagacaaaa caccgactg atcacccaa tcggaacag gagcggtcg tgtattctg ctagcgcag tttcagcga cgctccggt gactggatt tggatgcac tgcgtcctg caagtcaaa gaaagctcc gaaagcagt cagcagccc gtcatcgtc tgcaccaac caagtgctg agcgaggcc gaagcggcc gtcatgcgg accaacaac tggctctat cactattat tccgtgcaa aacaaactc cagtatggt ctcgcctat gacattgga ccaccctca cggctcctc tactacccg gaaaaccca cccgggcag gaattcacc gggttagtg gccaggcca gtaaaataa
- >TC013566 RA
actacgcga tcgtatttt tattctaaa actcacgtg ctttgtcca ttcgcgccg tttttcgta ctacaatgt gtgtgtgaa tgtgttatt tgtattttc attaactga caaacatgt tcacaaggt ttgatgcaa acaaatcga ccaaaggag ccagcaagc tacgaagag acctaataa atgcagaaa tagcaaatt tacgtgagt tattacctt taccaccat cgacgaggc aaaggcttt cacaactgc agcttatgg cactagtct gtgtatatg tcaggaaag ctaattatt ttcaacaag cattcaaat gccatgatg tgggattgc accagtcac caacaccca acatcggat tttcaaagg cattgaacg gctttctga tgatgatga ctcaaaatg gtaagctgt tgtatatat cggacaatg ctgcggagt acctgggac attccatgg aggacctgt tgatccatg gagatagtg tatacgata ttatagata agcaggatc accaagcaa ttcaatgcg aattgggac ggccagtaa attcaggat caaattcta gcactagtg aagaaattc gtttatttt tatgtagga tgaatgttt ctagaaacg caaggcggc aaatgaggt tcggggacc aaaaggtag tgctggtgc agggtcgat acttgtcgt ttttgccat tatgcagtc gaaacgaac cagtcttcc tggccacct gtacgccag tggcgatgc cagaaacca gagagtgtg tcgtgcagg gcgccacca acgtcttca cttcagtac actcgatgg atatgcgat ttatccaca tcgacaaca acggcgaat tctacttgg gttttccgc gcgccgatc tgcaggggg tgtcctggt accggctgc tgcattggg aatgcacgc gtgaggccc agacaaaac accgactga tcacccaat cggaacagg agcggtcgt gtattctgc tagcgcagt ttcagcgac gctccggtg actggattt ggatgcact gcgtcctgc aagtcaaag aaagctccg aaagcagtc agcagcccg tcatcgtct gcaccaacc aagtgctga gcgaggccg aagcggccg tcatgcgga ccaacaact ggctctatc actattatt ccgtgcaaa acaaactcc agtatggtc tcgcctatg acattggac caccctcac ggctcctct actacccgg aaaacccac ccgggcagg aattcaccg ggttagtgg ccaggccag taaaataac actttcatt tgaaaacgt ctagtccac gaatttttt cttttaacc accgacgaa aataaagtg tttttgata ct
- >TC013566 RB
actacgcga tcgtatttt tattctaaa actcacgtg ctttgtcca ttcgcgccg tttttcgta ctacaatgt gtgtgtgaa tgtgttatt tgtattttc attaactga caaacatgt tcacaaggt ttgatgcaa acaaatcga ccaaaggag ccagcaagc tacgaagag acctaataa atgcagaaa tagcaaatt tacgtgagt tattacctt taccaccat cgacgaggc aaaggcttt cacaactgc agcttatgg cactagtct gtgtatatg tcaggaaag ctaattatt ttcaacaag cattcaaat gccatgatg tgggattgc accagtcac caacaccca acatcggat tttcaaagg cattgaacg gctttctga tgatgatga ctcaaaatg gtaagctgt tgtatatat cggacaatg ctgcggagt acctgggac attccatgg tgagtgttt tacaggagg acctgttga tccatggag atagtgtat acgatatta tagataagc aggatcacc aagcaattc aatgcgaat tgggacggc cagtaaatt caggatcaa attctagca ctagtgaag aaattcgtt tatttttat gtaggatga atgtttcta gaaacgcaa ggcggcaaa tgaggttcg gggaccaaa aggtagtgc tggtgcagg gtcgatact tgtcgtttt tgccattat gcagtcgaa acgaaccag tcttcctgg ccacctgta cgccagtgg cgatgccag aaaccagag agtgtgtcg tgcagggcg ccaccaacg tcttcactt cagtacact cgatggata tgcgattta tccacatcg acaacaacg gcgaattct acttgggtt ttccgcgcg ccgatctgc agggggtgt cctggtacc ggctgctgc attgggaat gcacgcgtg aggcccaga caaaacacc gactgatca cccaatcgg aacaggagc ggtcgtgta ttctgctag cgcagtttc agcgacgct ccggtgact ggatttgga tgcactgcg tcctgcaag tcaaagaaa gctccgaaa gcagtcagc agcccgtca tcgtctgca ccaaccaag tgctgagcg aggccgaag cggccgtca tgcggacca acaactggc tctatcact attattccg tgcaaaaca aactccagt atggtctcg cctatgaca ttggaccac cctcacggc tcctctact acccggaaa acccacccg ggcaggaat tcaccgggt tagtggcca ggccagtaa aataacact ttcatttga aaacgtcta gtccacgaa ttttttctt ttaaccacc gacgaaaat aaagtgttt ttgatact
