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Gene information
General information
TC013566 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3608172..3628182
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013566
    iBeetle screen iB_06586

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    remarks: eggs lost,
    Adult morphology
    remarks: eggs lost,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     remarks: eggs lost,
    remarks - cuticles: later than schedule,
     
    8 days later, due to lost eggs in day 9
     

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013566 RA
      MFTRFDANK STKGASKLR RDLINAEIA NLRELLPLP 
PSTRQRLSQ LQLMALVCV YVRKANYFQ QAFKCHDVG 
LHQSPTPNI GFSKALNGF LMMMTQNGK LLYISDNAA 
EYLGHSMED LLIHGDSVY DIIDKQDHQ AIQCELGRP 
VNSGSNSST SEEIRLFLC RMNVSRNAR RQMRFGDQK 
VVLVQGRYL SFLPLCSRN EPVFLATCT PVAMPETRE 
CVVQGATNV FTSVHSMDM RFIHIDNNG EFYLGFPRA 
DLQGVSWYR LLHWECTRE AQTKHRLIT QSEQERSCI 
LLAQFQRRS GDWIWMHCV LQVKESSES SQQPVIVCT 
NQVLSEAEA AVMRTNNWL YHYYSVQNK LQYGLAYDI 
GPPSRLLYY PENPPGQEF TGLVARPVK
    • >TC013566 RB
      MFTRFDANK STKGASKLR RDLINAEIA NLRELLPLP 
PSTRQRLSQ LQLMALVCV YVRKANYFQ QAFKCHDVG 
LHQSPTPNI GFSKALNGF LMMMTQNGK LLYISDNAA 
EYLGHSMVS VLQEDLLIH GDSVYDIID KQDHQAIQC 
ELGRPVNSG SNSSTSEEI RLFLCRMNV SRNARRQMR 
FGDQKVVLV QGRYLSFLP LCSRNEPVF LATCTPVAM 
PETRECVVQ GATNVFTSV HSMDMRFIH IDNNGEFYL 
GFPRADLQG VSWYRLLHW ECTREAQTK HRLITQSEQ 
ERSCILLAQ FQRRSGDWI WMHCVLQVK ESSESSQQP 
VIVCTNQVL SEAEAAVMR TNNWLYHYY SVQNKLQYG 
LAYDIGPPS RLLYYPENP PGQEFTGLV ARPVK
    CDS sequence
    • >TC013566 RA
      atgttcaca aggtttgat gcaaacaaa tcgaccaaa 
ggagccagc aagctacga agagaccta ataaatgca 
gaaatagca aatttacgt gagttatta cctttacca 
ccatcgacg aggcaaagg ctttcacaa ctgcagctt 
atggcacta gtctgtgta tatgtcagg aaagctaat 
tattttcaa caagcattc aaatgccat gatgtggga 
ttgcaccag tcaccaaca cccaacatc ggattttca 
aaggcattg aacggcttt ctgatgatg atgactcaa 
aatggtaag ctgttgtat atatcggac aatgctgcg 
gagtacctg ggacattcc atggaggac ctgttgatc 
catggagat agtgtatac gatattata gataagcag 
gatcaccaa gcaattcaa tgcgaattg ggacggcca 
gtaaattca ggatcaaat tctagcact agtgaagaa 
attcgttta tttttatgt aggatgaat gtttctaga 
aacgcaagg cggcaaatg aggttcggg gaccaaaag 
gtagtgctg gtgcagggt cgatacttg tcgtttttg 
ccattatgc agtcgaaac gaaccagtc ttcctggcc 
acctgtacg ccagtggcg atgccagaa accagagag 
tgtgtcgtg cagggcgcc accaacgtc ttcacttca 
gtacactcg atggatatg cgatttatc cacatcgac 
aacaacggc gaattctac ttgggtttt ccgcgcgcc 
gatctgcag ggggtgtcc tggtaccgg ctgctgcat 
tgggaatgc acgcgtgag gcccagaca aaacaccga 
ctgatcacc caatcggaa caggagcgg tcgtgtatt 
ctgctagcg cagtttcag cgacgctcc ggtgactgg 
atttggatg cactgcgtc ctgcaagtc aaagaaagc 
tccgaaagc agtcagcag cccgtcatc gtctgcacc 
aaccaagtg ctgagcgag gccgaagcg gccgtcatg 
cggaccaac aactggctc tatcactat tattccgtg 
caaaacaaa ctccagtat ggtctcgcc tatgacatt 
ggaccaccc tcacggctc ctctactac ccggaaaac 
ccacccggg caggaattc accgggtta gtggccagg 
ccagtaaaa taa
    • >TC013566 RB
      atgttcaca aggtttgat gcaaacaaa tcgaccaaa 
ggagccagc aagctacga agagaccta ataaatgca 
gaaatagca aatttacgt gagttatta cctttacca 
ccatcgacg aggcaaagg ctttcacaa ctgcagctt 
atggcacta gtctgtgta tatgtcagg aaagctaat 
tattttcaa caagcattc aaatgccat gatgtggga 
ttgcaccag tcaccaaca cccaacatc ggattttca 
aaggcattg aacggcttt ctgatgatg atgactcaa 
aatggtaag ctgttgtat atatcggac aatgctgcg 
gagtacctg ggacattcc atggtgagt gttttacag 
gaggacctg ttgatccat ggagatagt gtatacgat 
attatagat aagcaggat caccaagca attcaatgc 
gaattggga cggccagta aattcagga tcaaattct 
agcactagt gaagaaatt cgtttattt ttatgtagg 
atgaatgtt tctagaaac gcaaggcgg caaatgagg 
ttcggggac caaaaggta gtgctggtg cagggtcga 
tacttgtcg tttttgcca ttatgcagt cgaaacgaa 
ccagtcttc ctggccacc tgtacgcca gtggcgatg 
ccagaaacc agagagtgt gtcgtgcag ggcgccacc 
aacgtcttc acttcagta cactcgatg gatatgcga 
tttatccac atcgacaac aacggcgaa ttctacttg 
ggttttccg cgcgccgat ctgcagggg gtgtcctgg 
taccggctg ctgcattgg gaatgcacg cgtgaggcc 
cagacaaaa caccgactg atcacccaa tcggaacag 
gagcggtcg tgtattctg ctagcgcag tttcagcga 
cgctccggt gactggatt tggatgcac tgcgtcctg 
caagtcaaa gaaagctcc gaaagcagt cagcagccc 
gtcatcgtc tgcaccaac caagtgctg agcgaggcc 
gaagcggcc gtcatgcgg accaacaac tggctctat 
cactattat tccgtgcaa aacaaactc cagtatggt 
ctcgcctat gacattgga ccaccctca cggctcctc 
tactacccg gaaaaccca cccgggcag gaattcacc 
gggttagtg gccaggcca gtaaaataa
    mRNA sequence
    • >TC013566 RA
      actacgcga tcgtatttt tattctaaa actcacgtg 
ctttgtcca ttcgcgccg tttttcgta ctacaatgt 
gtgtgtgaa tgtgttatt tgtattttc attaactga 
caaacatgt tcacaaggt ttgatgcaa acaaatcga 
ccaaaggag ccagcaagc tacgaagag acctaataa 
atgcagaaa tagcaaatt tacgtgagt tattacctt 
taccaccat cgacgaggc aaaggcttt cacaactgc 
agcttatgg cactagtct gtgtatatg tcaggaaag 
ctaattatt ttcaacaag cattcaaat gccatgatg 
tgggattgc accagtcac caacaccca acatcggat 
tttcaaagg cattgaacg gctttctga tgatgatga 
ctcaaaatg gtaagctgt tgtatatat cggacaatg 
ctgcggagt acctgggac attccatgg aggacctgt 
tgatccatg gagatagtg tatacgata ttatagata 
agcaggatc accaagcaa ttcaatgcg aattgggac 
ggccagtaa attcaggat caaattcta gcactagtg 
aagaaattc gtttatttt tatgtagga tgaatgttt 
ctagaaacg caaggcggc aaatgaggt tcggggacc 
aaaaggtag tgctggtgc agggtcgat acttgtcgt 
ttttgccat tatgcagtc gaaacgaac cagtcttcc 
tggccacct gtacgccag tggcgatgc cagaaacca 
gagagtgtg tcgtgcagg gcgccacca acgtcttca 
cttcagtac actcgatgg atatgcgat ttatccaca 
tcgacaaca acggcgaat tctacttgg gttttccgc 
gcgccgatc tgcaggggg tgtcctggt accggctgc 
tgcattggg aatgcacgc gtgaggccc agacaaaac 
accgactga tcacccaat cggaacagg agcggtcgt 
gtattctgc tagcgcagt ttcagcgac gctccggtg 
actggattt ggatgcact gcgtcctgc aagtcaaag 
aaagctccg aaagcagtc agcagcccg tcatcgtct 
gcaccaacc aagtgctga gcgaggccg aagcggccg 
tcatgcgga ccaacaact ggctctatc actattatt 
ccgtgcaaa acaaactcc agtatggtc tcgcctatg 
acattggac caccctcac ggctcctct actacccgg 
aaaacccac ccgggcagg aattcaccg ggttagtgg 
ccaggccag taaaataac actttcatt tgaaaacgt 
ctagtccac gaatttttt cttttaacc accgacgaa 
aataaagtg tttttgata ct
    • >TC013566 RB
      actacgcga tcgtatttt tattctaaa actcacgtg 
ctttgtcca ttcgcgccg tttttcgta ctacaatgt 
gtgtgtgaa tgtgttatt tgtattttc attaactga 
caaacatgt tcacaaggt ttgatgcaa acaaatcga 
ccaaaggag ccagcaagc tacgaagag acctaataa 
atgcagaaa tagcaaatt tacgtgagt tattacctt 
taccaccat cgacgaggc aaaggcttt cacaactgc 
agcttatgg cactagtct gtgtatatg tcaggaaag 
ctaattatt ttcaacaag cattcaaat gccatgatg 
tgggattgc accagtcac caacaccca acatcggat 
tttcaaagg cattgaacg gctttctga tgatgatga 
ctcaaaatg gtaagctgt tgtatatat cggacaatg 
ctgcggagt acctgggac attccatgg tgagtgttt 
tacaggagg acctgttga tccatggag atagtgtat 
acgatatta tagataagc aggatcacc aagcaattc 
aatgcgaat tgggacggc cagtaaatt caggatcaa 
attctagca ctagtgaag aaattcgtt tatttttat 
gtaggatga atgtttcta gaaacgcaa ggcggcaaa 
tgaggttcg gggaccaaa aggtagtgc tggtgcagg 
gtcgatact tgtcgtttt tgccattat gcagtcgaa 
acgaaccag tcttcctgg ccacctgta cgccagtgg 
cgatgccag aaaccagag agtgtgtcg tgcagggcg 
ccaccaacg tcttcactt cagtacact cgatggata 
tgcgattta tccacatcg acaacaacg gcgaattct 
acttgggtt ttccgcgcg ccgatctgc agggggtgt 
cctggtacc ggctgctgc attgggaat gcacgcgtg 
aggcccaga caaaacacc gactgatca cccaatcgg 
aacaggagc ggtcgtgta ttctgctag cgcagtttc 
agcgacgct ccggtgact ggatttgga tgcactgcg 
tcctgcaag tcaaagaaa gctccgaaa gcagtcagc 
agcccgtca tcgtctgca ccaaccaag tgctgagcg 
aggccgaag cggccgtca tgcggacca acaactggc 
tctatcact attattccg tgcaaaaca aactccagt 
atggtctcg cctatgaca ttggaccac cctcacggc 
tcctctact acccggaaa acccacccg ggcaggaat 
tcaccgggt tagtggcca ggccagtaa aataacact 
ttcatttga aaacgtcta gtccacgaa ttttttctt 
ttaaccacc gacgaaaat aaagtgttt ttgatact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY