Status
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Gene information
General information
TC031508
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014233,TC014234
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3513054..3519599
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031508
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06583

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    remarks: eggs lost,
    Adult morphology
    remarks: eggs lost,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: eggs lost,
    remarks - cuticles: later than schedule,
     
    8 days later, due to lost eggs in day 9
     

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031508 RA
      MLDRRTPSV RERDARAQP LSPRRDRSR DLSVLAPST 
      SSVTRDSSS ASSADFRDC GAVPVSECG SERLDPGHP 
      DSGHGPRLD RSGHVCRQL PSWPLIKMS CSVTERVTE 
      NRAGPGGGP ATGAGAPAG AGCRLRGSS SASLSTPPN 
      SRGGPPASA GSQSGGPQN GPQPGQGAP LRPKPCCLC 
      WCCCCSCSC STPGKGNDE NGPTRNSNN SDLLNYDGD 
      PPPSLEEIR SWGRSFDKL MRSPAGRKV FRDFLRCEY 
      SEENILFWL ACEDLKKEN NPEAVEEKA RIIYEDYIS 
      ILSPKEVSL DARVREIVN RNMVEPTPH TFDDAQLQI 
      YTLMHRDSY PRFVNSPLY RSLAQQQPP SQQQQQQQQ 
      QGEKDERSS IAPDQDGPG PHG
    CDS sequence
    • >TC031508 RA
      atgttggat cggcgcacg ccgtcggta cgcgaacgc 
      gacgcccga gcgcaacct ctcagtcca cgtagggac 
      cgttctcgc gatctctcc gtgctcgcg ccatcgacg 
      tccagcgtc actcgagac agcagcagc gcgtcgtcg 
      gccgacttc cgcgactgc ggcgcagtg ccagtgtcc 
      gagtgcggc agtgagcgc ctggaccct ggacatccg 
      gattccgga catggccct cggctcgac cgcagcggg 
      cacgtgtgc cgacagcta ccttcatgg ccattgata 
      aagatgtct tgtagcgta actgagcgg gtgacggag 
      aacagagcg gggcctggg ggtgggcca gcgaccggt 
      gcaggagct ccagctggg gcgggctgc cgacttcga 
      gggtccagc agtgcgagc ctttccacg cctccgaac 
      tccagagga gggcccccg gcctcggcg gggtcccag 
      tccggcggc ccccagaac ggcccccag cccggccag 
      ggcgctccg ctgcgcccc aagccctgc tgcctgtgc 
      tggtgctgc tgctgcagc tgttcctgt tcaacccct 
      ggaaaagga aacgatgaa aatggccct acaagaaat 
      tccaataac tcagatctt ttaaattac gatggagat 
      ccacctcct tctttagag gaaatcaga agttggggt 
      agatctttt gataaactt atgcgaagc cccgctgga 
      cgaaaagtt tttagagat ttcctacgg tgtgaatac 
      tcggaagaa aatatatta ttctggctg gcttgtgaa 
      gatttaaaa aaagaaaac aatcccgaa gctgttgaa 
      gagaaggca agaatcata tacgaagac tacatttcg 
      attctatca cctaaagag gtgtctttg gacgcccgc 
      gttcgcgag atcgtgaac cggaacatg gtggagccg 
      accccgcac acgttcgac gacgctcag ctgcagatt 
      tacacattg atgcatcga gatagctac ccccgtttt 
      gtgaattcg cccttgtac cggtcgctg gcgcagcag 
      cagcccccg tctcagcag cagcagcag cagcagcag 
      cagggcgag aaggacgag cgctcgtcg atagcgccg 
      gatcaggac gggccagga ccccacggg tag
    mRNA sequence
    • >TC031508 RA
      gtcgtctag tgaaagtag gtgtagcgt cgtagcatc 
      gtagcgtcg taggcgcgg gcgctttgg cggcgtcgg 
      tggcggcgt cggcatgcg ccatgttgg atcggcgca 
      cgccgtcgg tacgcgaac gcgacgccc gagcgcaac 
      ctctcagtc cacgtaggg accgttctc gcgatctct 
      ccgtgctcg cgccatcga cgtccagcg tcactcgag 
      acagcagca gcgcgtcgt cggccgact tccgcgact 
      gcggcgcag tgccagtgt ccgagtgcg gcagtgagc 
      gcctggacc ctggacatc cggattccg gacatggcc 
      ctcggctcg accgcagcg ggcacgtgt gccgacagc 
      taccttcat ggccattga taaagatgt cttgtagcg 
      taactgagc gggtgacgg agaacagag cggggcctg 
      ggggtgggc cagcgaccg gtgcaggag ctccagctg 
      gggcgggct gccgacttc gagggtcca gcagtgcga 
      gcctttcca cgcctccga actccagag gagggcccc 
      cggcctcgg cggggtccc agtccggcg gcccccaga 
      acggccccc agcccggcc agggcgctc cgctgcgcc 
      ccaagccct gctgcctgt gctggtgct gctgctgca 
      gctgttcct gttcaaccc ctggaaaag gaaacgatg 
      aaaatggcc ctacaagaa attccaata actcagatc 
      ttttaaatt acgatggag atccacctc cttctttag 
      aggaaatca gaagttggg gtagatctt ttgataaac 
      ttatgcgaa gccccgctg gacgaaaag tttttagag 
      atttcctac ggtgtgaat actcggaag aaaatatat 
      tattctggc tggcttgtg aagatttaa aaaaagaaa 
      acaatcccg aagctgttg aagagaagg caagaatca 
      tatacgaag actacattt cgattctat cacctaaag 
      aggtgtctt tggacgccc gcgttcgcg agatcgtga 
      accggaaca tggtggagc cgaccccgc acacgttcg 
      acgacgctc agctgcaga tttacacat tgatgcatc 
      gagatagct acccccgtt ttgtgaatt cgcccttgt 
      accggtcgc tggcgcagc agcagcccc cgtctcagc 
      agcagcagc agcagcagc agcagggcg agaaggacg 
      agcgctcgt cgatagcgc cggatcagg acgggccag 
      gaccccacg ggtagcgtt cttccggcc tttgacggc 
      ggcctaacg agacctaga ctcgacagt cgacaataa 
      ctcggaagt cggaaggcg gcggctacg gccaggcgc 
      ctcttcgga tcattcctc gctccgaca ccgatgcac 
      cgtcagttc caaaagtgc gaccgtgga ccggtgtcg 
      tcaacggac caatcccgg atcgggaca gtgacgtta 
      caaaccgtt attttgtta ccgcgtgca tttcatagc 
      aatcagact tggacgtca ctcgtcgct cacttctta 
      aatattagt gttgtgtaa taacgaagt tatggttca 
      tctcacttc taggattag gataatgtg gcataagat 
      taagtaata ataagggct acttgtctc actagtcaa 
      cccacccca tcccgccgc cgctcgccg cgacttaca 
      atatgaaga tgtgatata actacgtag cgactcgac 
      cacggcggt cagccctgc cctcattct acttgcacc 
      acaccatgt accccaatt atagtacac taataatcg 
      acacactgt gaattcata taaataaat atcaaatat 
      acgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY