Status
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Gene information
General information
TC031501
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030586
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3135259..3152696
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031501
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06580

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031501 RA
      MLSPKMQRT IRVNENQLI MLSERAHYD HSQAGYLHK 
      RSADSSKWQ LRWFVLYQN LLFYYDSEN CHRPSGVLL 
      LEGCYCERL ITTGSASKT KDGSEKQYC FAISYRREN 
      IRQYELRAL NEMECKNWI EAIRDASFN KLLLQKEEL 
      EQKHLHLLQ IVESEKTAK WQYTQQCEE LASEIKKLR 
      AELFALKKE WKPIPTHQS TSSLTSNQG TSTSTVSGP 
      FAHVEQDSA ELRKIKKVQ SFFRGWLCR RRWKQIVEQ 
      YIKSPHAES MRKRNSLVF RMVEAEEEY MEQMEVLVS 
      CFLRPFKMA ASSKKPPCS HEDVNSIFL NSETVLFLH 
      QIFLKGLTS RLESWPTLV LGDLFSMLL PMLSIYQEY 
      VRNHHYSLQ VLTECKQSP QFSAMLKRL ESKPACHGR 
      SLETFLTYP MHQVPRYII TLHELLAHT PHDHVERKS 
      LQNARQQLE DLSRQMHDE VSETENLRK NLAVERMVV 
      EGCDILLDV NQVFVRQGS LIQLPTEKH RSSRSKLNF 
      KSEKECVRQ CFLFSNHLI IATRTSSGK LHLVPDIGK 
      IPLADALLI EDPSDNPED DESSVCSVS SQSSGISET 
      SAASCSLHN RDFKIIVDL KDGSQPGQV TVHLLASSM 
      QEKAAWISD ISQCLDNVQ FNDMIRPTM SDNSSITLP 
      QSLKNDPKL FKDEADIRF SRTMNSCKV PQIRYATPE 
      RLLQRLTDL RFLSIDFLN TFLLTYRVF TDGVTVLEA 
      LKKVFYSSD PEPQGSVIS LEVFSNKDE NTLQLYDDR 
      RRSSFSPRR TSGASSVSG YGSEMSERD RSHSYDSQG 
      HRHWKATLQ KYEEESHEQ THPRIIRQS PTAQHKDSV 
      SPPVQKSIS IRVEQSETP KEDKTHLAI PKVAASSSS 
      ETLTDVTVL SAPCSPSNL SSVTLVGSS PSDTTEGET 
      GTSPKKPPS PKKEPSPTK EPPHQRPSI VERKVERGT 
      SEDTEEDMT QFFNTMHRA SCSSSRSAS ISTTTTSAV 
      LQSYYGSRR SMQEGEATS TARSSFQHD SPQKPSKAG 
      VVITSFRQS KRRSSTSSA AAAFAIATS GSSNPRDLS 
      PSARGGGMM DQDEFAMER NRYKENVIS TACTMRVLN 
      VLRHWISKH AQDFENDHK LKNLTIEFL DDIIYSPNL 
      LPAEHKAAA QLLRLLTKE ESESSKVDI FQLVSAPST 
      PSKESIETL SALEIAEQL TYIDHQIFI SISSEEFLG 
      QAWMTNDKT SRAPHILLM TKRFNDVSC LVASEILRR 
      PSLCARVAA IEKWAAVAD ISRCLHNFN GVLQICSAF 
      TNSSVFRLK KTWDKVSKT TRQTIEKLQ NIVSSDGRF 
      RSLRDALHR CDPPCIPYL GLYLTDLSF IEEGTPNFT 
      QDGLLNFSK MRMIAHVIR EIRHFQQTP YKIELIPKV 
      ANYLLDSSL WMDDEELYN TSLEIEPRT SRLSTSALG 
      QLSLSSKS
    • >TC031501 RB
      MLSPKMQRT IRVNENQLI MLSERAHYD HSQAGYLHK 
      RSADSSKWQ LRWFVLYQN LLFYYDSEN CHRPSGVLL 
      LEGCYCERL ITTGSASKT KDGSEKQYC FAISYRREN 
      IRQYELRAL NEMECKNWI EAIRDASFN KLLLQKEEL 
      EQKHLHLLQ IVESEKTAK WQYTQQCEE LASEIKKLR 
      AELFALKKE WKPIPTHQS TSSLTSNQG TSTSTVSGP 
      FAHVEQDSA ELRKIKKVQ SFFRGWLCR RRWKQIVEQ 
      YIKSPHAES MRKRNSLVF RMVEAEEEY MEQMEVLVS 
      CFLRPFKMA ASSKKPPCS HEDVNSIFL NSETVLFLH 
      QIFLKGLTS RLESWPTLV LGDLFSMLL PMLSIYQEY 
      VRNHHYSLQ VLTECKQSP QFSAMLKRL ESKPACHGR 
      SLETFLTYP MHQVPRYII TLHELLAHT PHDHVERKS 
      LQNARQQLE DLSRQMHDE VSETENLRK NLAVERMVV 
      EGCDILLDV NQVFVRQGS LIQLPTEKH RSSRSKLNF 
      KSEKECVRQ CFLFSNHLI IATRTSSGK LHLVPDIGK 
      IPLADALLI EDPSDNPED DESSVCSVS SQSSGISET 
      SAASCSLHN RDFKIIVDL KDGSQPGQV TVHLLASSM 
      QEKAAWISD ISQCLDNVQ FNDMIRPTM SDNSSITLP 
      QSLKNDPKL FKDEADIRF SRTMNSCKV PQIRYATPE 
      RLLQRLTDL RFLSIDFLN TFLLTYRVF TDGVTVLEA 
      LKKVFYSSD PEPQGSVIS LEVFSNKDE NTLQLYDDR 
      RRSSFSPRR TSGASSVSG YGSEMSERD RSHSYDSQG 
      HRHWKATLQ KYEEESHEQ THPRIIRQS PTAQHKDSV 
      SPPVQKSIS IRVEQSETP KEDKTHLAI PKVAASSSS 
      ETLTDVTVL SAPCSPSNL SSVTLVGSS PSDTTEGET 
      GTSPKKPPS PKKEPSPTK EPPHQRPSI VERKVERGT 
      SEDTEDMTQ FFNTMHRAS CSSSRSASI STTTTSAVL 
      QSYYGSRRS MQEGEATST ARSSFQHDS PQKPSKAGV 
      VITSFRQSK RRSSTSSAA AAFAIATSG SSNPRDLSP 
      SARGGGMMD QDEFAMERN RYKENVIST ACTMRVLNV 
      LRHWISKHA QDFENDHKL KNLTIEFLD DIIYSPNLL 
      PAEHKAAAQ LLRLLTKEE SESSKVDIF QLVSAPSTP 
      SKESIETLS ALEIAEQLT YIDHQIFIS ISSEEFLGQ 
      AWMTNDKTS RAPHILLMT KRFNDVSCL VASEILRRP 
      SLCARVAAI EKWAAVADI SRCLHNFNG VLQICSAFT 
      NSSVFRLKK TWDKVSKTT RQTIEKLQN IVSSDGRFR 
      SLRDALHRC DPPCIPYLG LYLTDLSFI EEGTPNFTQ 
      DGLLNFSKM RMIAHVIRE IRHFQQTPY KIELIPKVA 
      NYLLDSSLW MDDEELYNT SLEIEPRTS RLSTSALGQ 
      LSLSSKS
    CDS sequence
    • >TC031501 RA
      atgctgagt ccgaagatg caaagaact atccgagtg 
      aacgagaat cagctgata atgttatca gaaagggct 
      cactatgac cactctcaa gccggctat ttgcataag 
      agatcagcg gacagcagt aaatggcag ctgaggtgg 
      ttcgtgctt taccagaac ttgctgttt tactacgat 
      tcggaaaat tgccatcga cccagcggc gtcctcctg 
      ctggaggga tgctactgc gagcgactt atcaccacc 
      ggaagcgcc tcaaagacc aaggatggg tccgagaag 
      cagtactgt ttcgctata tcgtacaga agagaaaat 
      attcgccag tacgagcta agagcttta aatgaaatg 
      gagtgtaaa aactggatt gaagctata cgagatgca 
      agttttaac aagttgctg ttgcaaaag gaagaactg 
      gaacagaaa catttgcat ttgttacaa atcgtcgaa 
      tcggaaaaa acagcaaag tggcaatat acacagcaa 
      tgcgaggag ttggcttct gaaattaaa aagctgcga 
      gctgagttg tttgctttg aaaaaagaa tggaagccc 
      attccaacg caccaatcg acgagcagt ttgacatca 
      aatcaaggg acatctaca tcgaccgtc tccggtcca 
      tttgctcac gttgagcaa gactcggcc gaattaaga 
      aaaattaag aaagttcaa agctttttc cgcggatgg 
      ctgtgtcgt cgacgttgg aaacaaatc gtcgaacaa 
      tacatcaaa agccctcat gctgaaagt atgagaaaa 
      cgcaacagt ctcgtattc cgcatggtt gaagccgaa 
      gaagagtac atggagcag atggaagtg ctcgtctcg 
      tgttttttg cgcccgttt aaaatggcc gcatcgtcg 
      aaaaaaccg ccatgttct cacgaagac gtcaactcg 
      atttttctg aacagcgag acggtgcta tttttacac 
      caaattttc ctcaaaggg ttaacatca agacttgaa 
      agctggcct accttagtt ttaggggat ttattttcc 
      atgctactt cctatgcta agcatctat caagagtac 
      gtcagaaac caccactac tcccttcaa gtactcacc 
      gagtgcaaa caatcaccg cagttttcg gctatgctt 
      aagcgactc gaaagcaaa cccgcctgc catggcaga 
      tccttagag actttcctc acatatcct atgcatcag 
      gttccgagg tacattatc accctccac gagctgcta 
      gcccacacc ccccacgac cacgtcgaa cgaaagagc 
      ctccagaac gcccgccaa cagctggag gacctctcc 
      agacaaatg cacgacgaa gtgtcggaa accgagaat 
      ttacgcaaa aacctggcc gttgagaga atggtcgtc 
      gaaggctgc gacatcctt ctcgacgtt aaccaagtt 
      ttcgtccgt caaggcagt ctaatccaa ctaccgacc 
      gaaaaacac agaagctcg agaagcaaa ttaaacttc 
      aaaagcgaa aaggaatgc gtccgacaa tgtttcttg 
      ttttcgaat catctcatc atcgccaca aggacttcg 
      agcggaaaa ctccattta gtcccagac atcggcaaa 
      ataccccta gctgacgcg ctcctgatc gaggatcca 
      agtgacaac cctgaagac gatgaatca tcggtttgt 
      tcagtttcg agccaatcg tcgggaatt tccgaaacg 
      tcggcggct agctgcagt ttgcacaac cgcgacttc 
      aaaatcatc gtcgacctg aaggacgga tcgcagcct 
      ggccaggtc acggtgcat ctgttggcg tcgtccatg 
      caggagaaa gccgcatgg atttccgat atcagccag 
      tgcttggat aatgtccaa tttaacgat atgatacgg 
      ccgacgatg tctgataac agctccatc actcttcct 
      caatcgctg aagaatgac ccaaagttg tttaaagac 
      gaggcagat atccggttc agtagaaca atgaactct 
      tgtaaagtt cctcaaata agatacgca acaccagaa 
      cgccttctt caaagacta actgattta agattcctc 
      tccatagac ttcttaaat acattcctc cttacatat 
      cgcgttttt accgacggc gtaactgtc ctggaagct 
      ttaaagaaa gtgttctat agtagtgat cccgaaccg 
      caaggctct gttatttcc ctcgaagta ttttcgaac 
      aaagacgaa aatacctta caattatac gatgaccga 
      agacgtagt agtttttcc ccgcgtaga acaagcggt 
      gctagctct gtctccggc tacggctca gaaatgagc 
      gaacgggac agatcccac agttacgac tctcaaggc 
      cacaggcat tggaaagca actttgcaa aaatacgag 
      gaagagagc cacgagcaa acccacccg agaatcatt 
      agacagagc ccgaccgca caacacaaa gactcagtt 
      tcgccccca gtccaaaag tccatctcc atcagagtc 
      gaacagtcc gaaactcca aaagaagac aaaacccac 
      ctcgccatc cccaaagtc gccgcgtcc agcagcagc 
      gaaacttta acagatgtg acagtgcta agtgctccc 
      tgctcgccc agcaaccta agctcggta acactagtc 
      ggctcctcc ccgtccgac accaccgag ggcgaaacc 
      ggaacttcg cccaaaaaa ccaccatca cccaagaaa 
      gaaccatca ccgaccaaa gagccccca caccagaga 
      ccctcaatc gtcgagcgc aaagtcgaa cgcggcact 
      tcggaggac accgaagaa gatatgaca cagtttttt 
      aacaccatg caccgggca tcgtgcagc tcctcgcga 
      tccgccagc atctccacc accaccacg agcgcagtc 
      ctccagagc tactacggg tcccggcgg tcaatgcaa 
      gagggcgaa gccacgtca accgcccgc tccagcttc 
      cagcacgac tccccccag aagcccagc aaggcaggg 
      gtggtcata acgtccttc cgacagtcg aagcgaagg 
      agcagcacc tccagcgcg gctgccgcc ttcgccatc 
      gccacctcc ggctcgagc aacccccgg gacctgtcc 
      ccgagcgcc cgcgggggc ggcatgatg gaccaagac 
      gagttcgcc atggaacgg aaccggtac aaggagaac 
      gtcatctcg accgcttgc accatgagg gttttgaac 
      gttttgaga cactggatc tcgaagcat gcgcaagac 
      ttcgaaaac gaccacaag ttgaagaac ttgacgatt 
      gagttcttg gatgatatt atctacagc cccaacttg 
      ctcccggcg gagcacaag gccgcagct cagctcttg 
      cgattgctg acgaaggag gagtccgag agctcgaaa 
      gtcgacatt ttccagctg gtttcggcg ccttcgacc 
      ccgagcaag gagagcatt gagacgctt tcagcgctt 
      gagatcgcg gagcagttg acgtacatt gaccaccag 
      atttttatc tcgatttcg agcgaggag ttcctggga 
      caggcttgg atgacgaac gataagacc agccgcgct 
      ccgcatatt ctcctgatg acgaagcgt tttaacgac 
      gtttcttgc ttggtggcg tcggagatc ctccgccgg 
      ccctctctg tgtgccagg gttgcggcg atagagaaa 
      tgggccgct gtggccgac atttcgcgc tgtttgcac 
      aattttaac ggtgtttta cagatctgt tcggcgttt 
      actaacagc tccgtgttc cgtttgaag aaaacttgg 
      gataaagtg tcgaaaacg acgcggcaa actatcgag 
      aaattgcaa aatatagtg tcgtcagat gggcggttc 
      aggtcgctg cgggacgct ctccacaga tgtgacccg 
      ccgtgtatc ccctacttg gggctttat ctaactgat 
      ttgtctttt atcgaagaa ggaacacca aattttaca 
      caagatggg ctcttgaac ttttctaag atgagaatg 
      attgctcat gttattcgg gagattcgt cactttcag 
      caaacccca tataaaatt gagttgata cctaaagtc 
      gccaactat ttgttggat tcgtcgttg tggatggat 
      gatgaagaa ctttacaat acctcgttg gaaatcgag 
      ccgcgaacc tcacgtctg agcacttca gctctgggg 
      cagttgtct ttatcgtca aaatcttaa
    • >TC031501 RB
      atgctgagt ccgaagatg caaagaact atccgagtg 
      aacgagaat cagctgata atgttatca gaaagggct 
      cactatgac cactctcaa gccggctat ttgcataag 
      agatcagcg gacagcagt aaatggcag ctgaggtgg 
      ttcgtgctt taccagaac ttgctgttt tactacgat 
      tcggaaaat tgccatcga cccagcggc gtcctcctg 
      ctggaggga tgctactgc gagcgactt atcaccacc 
      ggaagcgcc tcaaagacc aaggatggg tccgagaag 
      cagtactgt ttcgctata tcgtacaga agagaaaat 
      attcgccag tacgagcta agagcttta aatgaaatg 
      gagtgtaaa aactggatt gaagctata cgagatgca 
      agttttaac aagttgctg ttgcaaaag gaagaactg 
      gaacagaaa catttgcat ttgttacaa atcgtcgaa 
      tcggaaaaa acagcaaag tggcaatat acacagcaa 
      tgcgaggag ttggcttct gaaattaaa aagctgcga 
      gctgagttg tttgctttg aaaaaagaa tggaagccc 
      attccaacg caccaatcg acgagcagt ttgacatca 
      aatcaaggg acatctaca tcgaccgtc tccggtcca 
      tttgctcac gttgagcaa gactcggcc gaattaaga 
      aaaattaag aaagttcaa agctttttc cgcggatgg 
      ctgtgtcgt cgacgttgg aaacaaatc gtcgaacaa 
      tacatcaaa agccctcat gctgaaagt atgagaaaa 
      cgcaacagt ctcgtattc cgcatggtt gaagccgaa 
      gaagagtac atggagcag atggaagtg ctcgtctcg 
      tgttttttg cgcccgttt aaaatggcc gcatcgtcg 
      aaaaaaccg ccatgttct cacgaagac gtcaactcg 
      atttttctg aacagcgag acggtgcta tttttacac 
      caaattttc ctcaaaggg ttaacatca agacttgaa 
      agctggcct accttagtt ttaggggat ttattttcc 
      atgctactt cctatgcta agcatctat caagagtac 
      gtcagaaac caccactac tcccttcaa gtactcacc 
      gagtgcaaa caatcaccg cagttttcg gctatgctt 
      aagcgactc gaaagcaaa cccgcctgc catggcaga 
      tccttagag actttcctc acatatcct atgcatcag 
      gttccgagg tacattatc accctccac gagctgcta 
      gcccacacc ccccacgac cacgtcgaa cgaaagagc 
      ctccagaac gcccgccaa cagctggag gacctctcc 
      agacaaatg cacgacgaa gtgtcggaa accgagaat 
      ttacgcaaa aacctggcc gttgagaga atggtcgtc 
      gaaggctgc gacatcctt ctcgacgtt aaccaagtt 
      ttcgtccgt caaggcagt ctaatccaa ctaccgacc 
      gaaaaacac agaagctcg agaagcaaa ttaaacttc 
      aaaagcgaa aaggaatgc gtccgacaa tgtttcttg 
      ttttcgaat catctcatc atcgccaca aggacttcg 
      agcggaaaa ctccattta gtcccagac atcggcaaa 
      ataccccta gctgacgcg ctcctgatc gaggatcca 
      agtgacaac cctgaagac gatgaatca tcggtttgt 
      tcagtttcg agccaatcg tcgggaatt tccgaaacg 
      tcggcggct agctgcagt ttgcacaac cgcgacttc 
      aaaatcatc gtcgacctg aaggacgga tcgcagcct 
      ggccaggtc acggtgcat ctgttggcg tcgtccatg 
      caggagaaa gccgcatgg atttccgat atcagccag 
      tgcttggat aatgtccaa tttaacgat atgatacgg 
      ccgacgatg tctgataac agctccatc actcttcct 
      caatcgctg aagaatgac ccaaagttg tttaaagac 
      gaggcagat atccggttc agtagaaca atgaactct 
      tgtaaagtt cctcaaata agatacgca acaccagaa 
      cgccttctt caaagacta actgattta agattcctc 
      tccatagac ttcttaaat acattcctc cttacatat 
      cgcgttttt accgacggc gtaactgtc ctggaagct 
      ttaaagaaa gtgttctat agtagtgat cccgaaccg 
      caaggctct gttatttcc ctcgaagta ttttcgaac 
      aaagacgaa aatacctta caattatac gatgaccga 
      agacgtagt agtttttcc ccgcgtaga acaagcggt 
      gctagctct gtctccggc tacggctca gaaatgagc 
      gaacgggac agatcccac agttacgac tctcaaggc 
      cacaggcat tggaaagca actttgcaa aaatacgag 
      gaagagagc cacgagcaa acccacccg agaatcatt 
      agacagagc ccgaccgca caacacaaa gactcagtt 
      tcgccccca gtccaaaag tccatctcc atcagagtc 
      gaacagtcc gaaactcca aaagaagac aaaacccac 
      ctcgccatc cccaaagtc gccgcgtcc agcagcagc 
      gaaacttta acagatgtg acagtgcta agtgctccc 
      tgctcgccc agcaaccta agctcggta acactagtc 
      ggctcctcc ccgtccgac accaccgag ggcgaaacc 
      ggaacttcg cccaaaaaa ccaccatca cccaagaaa 
      gaaccatca ccgaccaaa gagccccca caccagaga 
      ccctcaatc gtcgagcgc aaagtcgaa cgcggcact 
      tcggaggac accgaagat atgacacag ttttttaac 
      accatgcac cgggcatcg tgcagctcc tcgcgatcc 
      gccagcatc tccaccacc accacgagc gcagtcctc 
      cagagctac tacgggtcc cggcggtca atgcaagag 
      ggcgaagcc acgtcaacc gcccgctcc agcttccag 
      cacgactcc ccccagaag cccagcaag gcaggggtg 
      gtcataacg tccttccga cagtcgaag cgaaggagc 
      agcacctcc agcgcggct gccgccttc gccatcgcc 
      acctccggc tcgagcaac ccccgggac ctgtccccg 
      agcgcccgc gggggcggc atgatggac caagacgag 
      ttcgccatg gaacggaac cggtacaag gagaacgtc 
      atctcgacc gcttgcacc atgagggtt ttgaacgtt 
      ttgagacac tggatctcg aagcatgcg caagacttc 
      gaaaacgac cacaagttg aagaacttg acgattgag 
      ttcttggat gatattatc tacagcccc aacttgctc 
      ccggcggag cacaaggcc gcagctcag ctcttgcga 
      ttgctgacg aaggaggag tccgagagc tcgaaagtc 
      gacattttc cagctggtt tcggcgcct tcgaccccg 
      agcaaggag agcattgag acgctttca gcgcttgag 
      atcgcggag cagttgacg tacattgac caccagatt 
      tttatctcg atttcgagc gaggagttc ctgggacag 
      gcttggatg acgaacgat aagaccagc cgcgctccg 
      catattctc ctgatgacg aagcgtttt aacgacgtt 
      tcttgcttg gtggcgtcg gagatcctc cgccggccc 
      tctctgtgt gccagggtt gcggcgata gagaaatgg 
      gccgctgtg gccgacatt tcgcgctgt ttgcacaat 
      tttaacggt gttttacag atctgttcg gcgtttact 
      aacagctcc gtgttccgt ttgaagaaa acttgggat 
      aaagtgtcg aaaacgacg cggcaaact atcgagaaa 
      ttgcaaaat atagtgtcg tcagatggg cggttcagg 
      tcgctgcgg gacgctctc cacagatgt gacccgccg 
      tgtatcccc tacttgggg ctttatcta actgatttg 
      tcttttatc gaagaagga acaccaaat tttacacaa 
      gatgggctc ttgaacttt tctaagatg agaatgatt 
      gctcatgtt attcgggag attcgtcac tttcagcaa 
      accccatat aaaattgag ttgatacct aaagtcgcc 
      aactatttg ttggattcg tcgttgtgg atggatgat 
      gaagaactt tacaatacc tcgttggaa atcgagccg 
      cgaacctca cgtctgagc acttcagct ctggggcag 
      ttgtcttta tcgtcaaaa tcttaa
    mRNA sequence
    • >TC031501 RA
      gaaactttt cttccaact tttcataat taattaagg 
      aaatatacg cattttcac tggaataaa gaaacctga 
      aaagatgct gagtccgaa gatgcaaag aactatccg 
      agtgaacga gaatcagct gataatgtt atcagaaag 
      ggctcacta tgaccactc tcaagccgg ctatttgca 
      taagagatc agcggacag cagtaaatg gcagctgag 
      gtggttcgt gctttacca gaacttgct gttttacta 
      cgattcgga aaattgcca tcgacccag cggcgtcct 
      cctgctgga gggatgcta ctgcgagcg acttatcac 
      caccggaag cgcctcaaa gaccaagga tgggtccga 
      gaagcagta ctgtttcgc tatatcgta cagaagaga 
      aaatattcg ccagtacga gctaagagc tttaaatga 
      aatggagtg taaaaactg gattgaagc tatacgaga 
      tgcaagttt taacaagtt gctgttgca aaaggaaga 
      actggaaca gaaacattt gcatttgtt acaaatcgt 
      cgaatcgga aaaaacagc aaagtggca atatacaca 
      gcaatgcga ggagttggc ttctgaaat taaaaagct 
      gcgagctga gttgtttgc tttgaaaaa agaatggaa 
      gcccattcc aacgcacca atcgacgag cagtttgac 
      atcaaatca agggacatc tacatcgac cgtctccgg 
      tccatttgc tcacgttga gcaagactc ggccgaatt 
      aagaaaaat taagaaagt tcaaagctt tttccgcgg 
      atggctgtg tcgtcgacg ttggaaaca aatcgtcga 
      acaatacat caaaagccc tcatgctga aagtatgag 
      aaaacgcaa cagtctcgt attccgcat ggttgaagc 
      cgaagaaga gtacatgga gcagatgga agtgctcgt 
      ctcgtgttt tttgcgccc gtttaaaat ggccgcatc 
      gtcgaaaaa accgccatg ttctcacga agacgtcaa 
      ctcgatttt tctgaacag cgagacggt gctattttt 
      acaccaaat tttcctcaa agggttaac atcaagact 
      tgaaagctg gcctacctt agttttagg ggatttatt 
      ttccatgct acttcctat gctaagcat ctatcaaga 
      gtacgtcag aaaccacca ctactccct tcaagtact 
      caccgagtg caaacaatc accgcagtt ttcggctat 
      gcttaagcg actcgaaag caaacccgc ctgccatgg 
      cagatcctt agagacttt cctcacata tcctatgca 
      tcaggttcc gaggtacat tatcaccct ccacgagct 
      gctagccca cacccccca cgaccacgt cgaacgaaa 
      gagcctcca gaacgcccg ccaacagct ggaggacct 
      ctccagaca aatgcacga cgaagtgtc ggaaaccga 
      gaatttacg caaaaacct ggccgttga gagaatggt 
      cgtcgaagg ctgcgacat ccttctcga cgttaacca 
      agttttcgt ccgtcaagg cagtctaat ccaactacc 
      gaccgaaaa acacagaag ctcgagaag caaattaaa 
      cttcaaaag cgaaaagga atgcgtccg acaatgttt 
      cttgttttc gaatcatct catcatcgc cacaaggac 
      ttcgagcgg aaaactcca tttagtccc agacatcgg 
      caaaatacc cctagctga cgcgctcct gatcgagga 
      tccaagtga caaccctga agacgatga atcatcggt 
      ttgttcagt ttcgagcca atcgtcggg aatttccga 
      aacgtcggc ggctagctg cagtttgca caaccgcga 
      cttcaaaat catcgtcga cctgaagga cggatcgca 
      gcctggcca ggtcacggt gcatctgtt ggcgtcgtc 
      catgcagga gaaagccgc atggatttc cgatatcag 
      ccagtgctt ggataatgt ccaatttaa cgatatgat 
      acggccgac gatgtctga taacagctc catcactct 
      tcctcaatc gctgaagaa tgacccaaa gttgtttaa 
      agacgaggc agatatccg gttcagtag aacaatgaa 
      ctcttgtaa agttcctca aataagata cgcaacacc 
      agaacgcct tcttcaaag actaactga tttaagatt 
      cctctccat agacttctt aaatacatt cctccttac 
      atatcgcgt ttttaccga cggcgtaac tgtcctgga 
      agctttaaa gaaagtgtt ctatagtag tgatcccga 
      accgcaagg ctctgttat ttccctcga agtattttc 
      gaacaaaga cgaaaatac cttacaatt atacgatga 
      ccgaagacg tagtagttt ttccccgcg tagaacaag 
      cggtgctag ctctgtctc cggctacgg ctcagaaat 
      gagcgaacg ggacagatc ccacagtta cgactctca 
      aggccacag gcattggaa agcaacttt gcaaaaata 
      cgaggaaga gagccacga gcaaaccca cccgagaat 
      cattagaca gagcccgac cgcacaaca caaagactc 
      agtttcgcc cccagtcca aaagtccat ctccatcag 
      agtcgaaca gtccgaaac tccaaaaga agacaaaac 
      ccacctcgc catccccaa agtcgccgc gtccagcag 
      cagcgaaac tttaacaga tgtgacagt gctaagtgc 
      tccctgctc gcccagcaa cctaagctc ggtaacact 
      agtcggctc ctccccgtc cgacaccac cgagggcga 
      aaccggaac ttcgcccaa aaaaccacc atcacccaa 
      gaaagaacc atcaccgac caaagagcc cccacacca 
      gagaccctc aatcgtcga gcgcaaagt cgaacgcgg 
      cacttcgga ggacaccga agaagatat gacacagtt 
      ttttaacac catgcaccg ggcatcgtg cagctcctc 
      gcgatccgc cagcatctc caccaccac cacgagcgc 
      agtcctcca gagctacta cgggtcccg gcggtcaat 
      gcaagaggg cgaagccac gtcaaccgc ccgctccag 
      cttccagca cgactcccc ccagaagcc cagcaaggc 
      aggggtggt cataacgtc cttccgaca gtcgaagcg 
      aaggagcag cacctccag cgcggctgc cgccttcgc 
      catcgccac ctccggctc gagcaaccc ccgggacct 
      gtccccgag cgcccgcgg gggcggcat gatggacca 
      agacgagtt cgccatgga acggaaccg gtacaagga 
      gaacgtcat ctcgaccgc ttgcaccat gagggtttt 
      gaacgtttt gagacactg gatctcgaa gcatgcgca 
      agacttcga aaacgacca caagttgaa gaacttgac 
      gattgagtt cttggatga tattatcta cagccccaa 
      cttgctccc ggcggagca caaggccgc agctcagct 
      cttgcgatt gctgacgaa ggaggagtc cgagagctc 
      gaaagtcga cattttcca gctggtttc ggcgccttc 
      gaccccgag caaggagag cattgagac gctttcagc 
      gcttgagat cgcggagca gttgacgta cattgacca 
      ccagatttt tatctcgat ttcgagcga ggagttcct 
      gggacaggc ttggatgac gaacgataa gaccagccg 
      cgctccgca tattctcct gatgacgaa gcgttttaa 
      cgacgtttc ttgcttggt ggcgtcgga gatcctccg 
      ccggccctc tctgtgtgc cagggttgc ggcgataga 
      gaaatgggc cgctgtggc cgacatttc gcgctgttt 
      gcacaattt taacggtgt tttacagat ctgttcggc 
      gtttactaa cagctccgt gttccgttt gaagaaaac 
      ttgggataa agtgtcgaa aacgacgcg gcaaactat 
      cgagaaatt gcaaaatat agtgtcgtc agatgggcg 
      gttcaggtc gctgcggga cgctctcca cagatgtga 
      cccgccgtg tatccccta cttggggct ttatctaac 
      tgatttgtc ttttatcga agaaggaac accaaattt 
      tacacaaga tgggctctt gaacttttc taagatgag 
      aatgattgc tcatgttat tcgggagat tcgtcactt 
      tcagcaaac cccatataa aattgagtt gatacctaa 
      agtcgccaa ctatttgtt ggattcgtc gttgtggat 
      ggatgatga agaacttta caatacctc gttggaaat 
      cgagccgcg aacctcacg tctgagcac ttcagctct 
      ggggcagtt gtctttatc gtcaaaatc ttaactctt 
      aacaataat cggcaatct tatggacgc gacaatcga 
      aacgatctg ctctttaaa acccttaca atcatcgtt 
      ttataaaat aattgaggt aataatgat atagagtta 
      attgaattt tagttcgcc agttactta ccgtcgtac 
      gtaactaac attgcaaaa caatcataa caattaagt 
      cactgtcat taagtaacc taaactctc ctgaggaag 
      tcgaaccct tgactctaa gactgttag cagttatat 
      atcattagg ggaaagcgt aggaaatct gtcaaatta 
      tataaagtg tcttcatag atgctactc tatatcata 
      aggcctaca tatcaagat ttccttagg aggcgaaaa 
      ctgacttgt tcatgcttg ggaaaacaa tggttgttg 
      tagcgtaga ttaccaaca attctccct caaaatttg 
      tgtttcaca ggtgttcag gatcccaaa aatggttca 
      ttgtgacta cacctgacg ctaaacaaa aaaccattt 
      ttgggattg tatctgtta gttctaaat agtatcaaa 
      tgtgataat agtgtgcgc aaattatat ctacatgta 
      gttgtacaa tattgttgg ctttttgtt gttaaaatg 
      ccgtcatat ctttgaaac atatctggg ctaatatta 
      cacttcaga cttcacaat aatgttatg tgatttacg 
      gatgtcttc tattgagtt accggtttc acattgtta 
      agtggcggt ttgccaaat tctagacgt aattagtgt 
      aacaaacta aaataatgt gccatcctt taacattta 
      atgtagttt tatgaagtt tattttgta gtaaatggg 
      atgtttaag taaatgtta gacacaata gatcaagtt 
      gtgtcactc tttagttca agccgatga ttttttcat 
      tttaccaaa tgtgttagt gctcaatta ttagtcaat 
      tatttgttt tgaaacacg tggcaacca cattttgct 
      cacttcagc acacttgtg catttacaa tctaaatgt 
      ttttttccg aaataatca attttaaat atttgtcaa 
      ttcgatcag actttgcta tcataatac tattgagca 
      cttatgttt acgactaaa tgttacatt gatagttca 
      taggaaaca gaattttta aataaattc acaaaaata 
      gatacgctt accttgata ttttactcc cgcaacagt 
      tgtagttca tgctaagtg gactacaat ttaccttag 
      ctacgcgag ccaaaattc aacaataga tttttgtaa 
      aaactctcg tttaaacga tacaataat tctctatca 
      agctgtttt tattacaat gttaataac tttaatatg 
      tacataaat ataaatata tttctttac aatct
    • >TC031501 RB
      acccatctt tgagagaaa attttcgtc aaataaaca 
      tgaataaag aaacctgaa aagatgctg agtccgaag 
      atgcaaaga actatccga gtgaacgag aatcagctg 
      ataatgtta tcagaaagg gctcactat gaccactct 
      caagccggc tatttgcat aagagatca gcggacagc 
      agtaaatgg cagctgagg tggttcgtg ctttaccag 
      aacttgctg ttttactac gattcggaa aattgccat 
      cgacccagc ggcgtcctc ctgctggag ggatgctac 
      tgcgagcga cttatcacc accggaagc gcctcaaag 
      accaaggat gggtccgag aagcagtac tgtttcgct 
      atatcgtac agaagagaa aatattcgc cagtacgag 
      ctaagagct ttaaatgaa atggagtgt aaaaactgg 
      attgaagct atacgagat gcaagtttt aacaagttg 
      ctgttgcaa aaggaagaa ctggaacag aaacatttg 
      catttgtta caaatcgtc gaatcggaa aaaacagca 
      aagtggcaa tatacacag caatgcgag gagttggct 
      tctgaaatt aaaaagctg cgagctgag ttgtttgct 
      ttgaaaaaa gaatggaag cccattcca acgcaccaa 
      tcgacgagc agtttgaca tcaaatcaa gggacatct 
      acatcgacc gtctccggt ccatttgct cacgttgag 
      caagactcg gccgaatta agaaaaatt aagaaagtt 
      caaagcttt ttccgcgga tggctgtgt cgtcgacgt 
      tggaaacaa atcgtcgaa caatacatc aaaagccct 
      catgctgaa agtatgaga aaacgcaac agtctcgta 
      ttccgcatg gttgaagcc gaagaagag tacatggag 
      cagatggaa gtgctcgtc tcgtgtttt ttgcgcccg 
      tttaaaatg gccgcatcg tcgaaaaaa ccgccatgt 
      tctcacgaa gacgtcaac tcgattttt ctgaacagc 
      gagacggtg ctattttta caccaaatt ttcctcaaa 
      gggttaaca tcaagactt gaaagctgg cctacctta 
      gttttaggg gatttattt tccatgcta cttcctatg 
      ctaagcatc tatcaagag tacgtcaga aaccaccac 
      tactccctt caagtactc accgagtgc aaacaatca 
      ccgcagttt tcggctatg cttaagcga ctcgaaagc 
      aaacccgcc tgccatggc agatcctta gagactttc 
      ctcacatat cctatgcat caggttccg aggtacatt 
      atcaccctc cacgagctg ctagcccac accccccac 
      gaccacgtc gaacgaaag agcctccag aacgcccgc 
      caacagctg gaggacctc tccagacaa atgcacgac 
      gaagtgtcg gaaaccgag aatttacgc aaaaacctg 
      gccgttgag agaatggtc gtcgaaggc tgcgacatc 
      cttctcgac gttaaccaa gttttcgtc cgtcaaggc 
      agtctaatc caactaccg accgaaaaa cacagaagc 
      tcgagaagc aaattaaac ttcaaaagc gaaaaggaa 
      tgcgtccga caatgtttc ttgttttcg aatcatctc 
      atcatcgcc acaaggact tcgagcgga aaactccat 
      ttagtccca gacatcggc aaaataccc ctagctgac 
      gcgctcctg atcgaggat ccaagtgac aaccctgaa 
      gacgatgaa tcatcggtt tgttcagtt tcgagccaa 
      tcgtcggga atttccgaa acgtcggcg gctagctgc 
      agtttgcac aaccgcgac ttcaaaatc atcgtcgac 
      ctgaaggac ggatcgcag cctggccag gtcacggtg 
      catctgttg gcgtcgtcc atgcaggag aaagccgca 
      tggatttcc gatatcagc cagtgcttg gataatgtc 
      caatttaac gatatgata cggccgacg atgtctgat 
      aacagctcc atcactctt cctcaatcg ctgaagaat 
      gacccaaag ttgtttaaa gacgaggca gatatccgg 
      ttcagtaga acaatgaac tcttgtaaa gttcctcaa 
      ataagatac gcaacacca gaacgcctt cttcaaaga 
      ctaactgat ttaagattc ctctccata gacttctta 
      aatacattc ctccttaca tatcgcgtt tttaccgac 
      ggcgtaact gtcctggaa gctttaaag aaagtgttc 
      tatagtagt gatcccgaa ccgcaaggc tctgttatt 
      tccctcgaa gtattttcg aacaaagac gaaaatacc 
      ttacaatta tacgatgac cgaagacgt agtagtttt 
      tccccgcgt agaacaagc ggtgctagc tctgtctcc 
      ggctacggc tcagaaatg agcgaacgg gacagatcc 
      cacagttac gactctcaa ggccacagg cattggaaa 
      gcaactttg caaaaatac gaggaagag agccacgag 
      caaacccac ccgagaatc attagacag agcccgacc 
      gcacaacac aaagactca gtttcgccc ccagtccaa 
      aagtccatc tccatcaga gtcgaacag tccgaaact 
      ccaaaagaa gacaaaacc cacctcgcc atccccaaa 
      gtcgccgcg tccagcagc agcgaaact ttaacagat 
      gtgacagtg ctaagtgct ccctgctcg cccagcaac 
      ctaagctcg gtaacacta gtcggctcc tccccgtcc 
      gacaccacc gagggcgaa accggaact tcgcccaaa 
      aaaccacca tcacccaag aaagaacca tcaccgacc 
      aaagagccc ccacaccag agaccctca atcgtcgag 
      cgcaaagtc gaacgcggc acttcggag gacaccgaa 
      gatatgaca cagtttttt aacaccatg caccgggca 
      tcgtgcagc tcctcgcga tccgccagc atctccacc 
      accaccacg agcgcagtc ctccagagc tactacggg 
      tcccggcgg tcaatgcaa gagggcgaa gccacgtca 
      accgcccgc tccagcttc cagcacgac tccccccag 
      aagcccagc aaggcaggg gtggtcata acgtccttc 
      cgacagtcg aagcgaagg agcagcacc tccagcgcg 
      gctgccgcc ttcgccatc gccacctcc ggctcgagc 
      aacccccgg gacctgtcc ccgagcgcc cgcgggggc 
      ggcatgatg gaccaagac gagttcgcc atggaacgg 
      aaccggtac aaggagaac gtcatctcg accgcttgc 
      accatgagg gttttgaac gttttgaga cactggatc 
      tcgaagcat gcgcaagac ttcgaaaac gaccacaag 
      ttgaagaac ttgacgatt gagttcttg gatgatatt 
      atctacagc cccaacttg ctcccggcg gagcacaag 
      gccgcagct cagctcttg cgattgctg acgaaggag 
      gagtccgag agctcgaaa gtcgacatt ttccagctg 
      gtttcggcg ccttcgacc ccgagcaag gagagcatt 
      gagacgctt tcagcgctt gagatcgcg gagcagttg 
      acgtacatt gaccaccag atttttatc tcgatttcg 
      agcgaggag ttcctggga caggcttgg atgacgaac 
      gataagacc agccgcgct ccgcatatt ctcctgatg 
      acgaagcgt tttaacgac gtttcttgc ttggtggcg 
      tcggagatc ctccgccgg ccctctctg tgtgccagg 
      gttgcggcg atagagaaa tgggccgct gtggccgac 
      atttcgcgc tgtttgcac aattttaac ggtgtttta 
      cagatctgt tcggcgttt actaacagc tccgtgttc 
      cgtttgaag aaaacttgg gataaagtg tcgaaaacg 
      acgcggcaa actatcgag aaattgcaa aatatagtg 
      tcgtcagat gggcggttc aggtcgctg cgggacgct 
      ctccacaga tgtgacccg ccgtgtatc ccctacttg 
      gggctttat ctaactgat ttgtctttt atcgaagaa 
      ggaacacca aattttaca caagatggg ctcttgaac 
      ttttctaag atgagaatg attgctcat gttattcgg 
      gagattcgt cactttcag caaacccca tataaaatt 
      gagttgata cctaaagtc gccaactat ttgttggat 
      tcgtcgttg tggatggat gatgaagaa ctttacaat 
      acctcgttg gaaatcgag ccgcgaacc tcacgtctg 
      agcacttca gctctgggg cagttgtct ttatcgtca 
      aaatcttaa ctcttaaca ataatcggc aatcttatg 
      gacgcgaca atcgaaacg atctgctct ttaaaaccc 
      ttacaatca tcgttttat aaaataatt gaggtaata 
      atgatatag agttaattg aattttagt tcgccagtt 
      acttaccgt cgtacgtaa ctaacattg caaaacaat 
      cataacaat taagtcact gtcattaag taacctaaa 
      ctctcctga ggaagtcga acccttgac tctaagact 
      gttagcagt tatatatca ttaggggaa agcgtagga 
      aatctgtca aattatata aagtgtctt catagatgc 
      tactctata tcataaggc ctacatatc aagatttcc 
      ttaggaggc gaaaactga cttgttcat gcttgggaa 
      aacaatggt tgttgtagc gtagattac caacaattc 
      tccctcaaa atttgtgtt tcacaggtg ttcaggatc 
      ccaaaaatg gttcattgt gactacacc tgacgctaa 
      acaaaaaac catttttgg gattgtatc tgttagttc 
      taaatagta tcaaatgtg ataatagtg tgcgcaaat 
      tatatctac atgtagttg tacaatatt gttggcttt 
      ttgttgtta aaatgccgt catatcttt gaaacatat 
      ctgggctaa tattacact tcagacttc acaataatg 
      ttatgtgat ttacggatg tcttctatt gagttaccg 
      gtttcacat tgttaagtg gcggtttgc caaattcta 
      gacgtaatt agtgtaaca aactaaaat aatgtgcca 
      tcctttaac atttaatgt agttttatg aagtttatt 
      ttgtagtaa atgggatgt ttaagtaaa tgttagaca 
      caatagatc aagttgtgt cactcttta gttcaagcc 
      gatgatttt ttcatttta ccaaatgtg ttagtgctc 
      aattattag tcaattatt tgttttgaa acacgtggc 
      aaccacatt ttgctcact tcagcacac ttgtgcatt 
      tacaatcta aatgttttt ttccgaaat aatcaattt 
      taaatattt gtcaattcg atcagactt tgctatcat 
      aatactatt gagcactta tgtttacga ctaaatgtt 
      acattgata gttcatagg aaacagaat ttttaaata 
      aattcacaa aaatagata cgcttacct tgatatttt 
      actcccgca acagttgta gttcatgct aagtggact 
      acaatttac cttagctac gcgagccaa aattcaaca 
      atagatttt tgtaaaaac tctcgttta aacgataca 
      ataattctc tatcaagct gtttttatt acaatgtta 
      ataacttta atatgtaca taaatataa atatatttc 
      tttacaatc t
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