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Gene information
General information
TC014200 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3072788..3090417
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014200
    iBeetle screen iB_06579

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014200 RA
      MTQHSDASS DRSEVSVTP PPPVSKMLE FASQQNLSF 
LNIEHRNML AAPLAALHS MTEMKQHQH QHHLSPDHL 
HGPEKADDV LLERTSLHV ERSGLTSRS PLGATSNSN 
SSQGANPHG IDHILSRPS QITTGPLHF PHSLGFTQN 
TLGMASNSA QTASNSGLR GFNIAAAAF FHHHAANSV 
NKPPVELGR STGGLYWPG FQGLVSNPI AWRDRLSSK 
QDAYFKFVN QGNVGGIGP DKDTKKKHT RPTFSGQQI 
FALEKTFEQ TKYLAGPER AKLAYALGM TESQVKVWF 
QNRRTKWRK KHAAEMATA KRKQEVLDN QTDENSDVI 
SDTEDEHAA KRQNIQ
    • >TC014200 RB
      MTQHSDASS DRSEVSVTP PPPVSKMLE FASQQNLSF 
LNIEHRNML AAPLAALHS MTEMKQHQH QHHLSPDHL 
HGPEKADDV LLERTSLHV ERSGLTSRS PLGATSNSN 
SSQGANPHG IDHILSRPS QITTGPLHF PHSLGFTQN 
TLGMASNSA QTASNSGLR GFNIAAAAF FHHHAANSV 
NKPPVELGR STGGLYWPG FQGLVSNPI AWRDRLSSM 
NQGNVGGIG PDKDTKKKH TRPTFSGQQ IFALEKTFE 
QTKYLAGPE RAKLAYALG MTESQVKVW FQNRRTKWR 
KKHAAEMAT AKRKQEVLD NQTDENSDV ISDTEDEHA 
AKRQNIQ
    CDS sequence
    • >TC014200 RA
      atgacgcaa cacagcgac gcgtccagc gaccgatcc 
gaagtctcc gtgacgccg ccaccgcca gtctccaaa 
atgctggag ttcgccagc cagcagaac ctcagcttc 
ctcaacatt gaacaccgg aacatgctc gccgccccg 
ctggcggcg ctccactcc atgacggag atgaagcaa 
caccaacac cagcaccac ctcagcccg gaccacctg 
cacggcccc gagaaagcc gacgatgtc ctgctggag 
cgtacaagt ttacacgta gaacgctcc ggcttgacc 
tcgcgcagc cccctgggg gccacgagc aacagcaat 
agctcccaa ggagccaac ccgcacggc atagaccac 
atactctcg cgaccctcg caaatcacg acgggcccg 
ctgcacttc ccgcacagc ctggggttc acgcagaac 
accctgggg atggcctcc aacagcgcg cagacggcc 
tcaaactcg ggcctacga gggttcaac atcgccgcc 
gctgccttc ttccatcac cacgccgcc aactccgtc 
aacaagccg cccgttgag ctcggaaga agcaccggc 
ggcctgtac tggcccggc ttccaggga ctcgtctcc 
aaccccatc gcttggcgg gatcggctc agcagcaaa 
caagatgct tactttaaa tttgtgaat cagggcaat 
gtgggagga attgggccg gacaaggac acgaaaaag 
aagcacacg aggcctact ttctcagga caacaaatt 
ttcgccctg gaaaaaacc ttcgaacaa accaaatac 
ttggcagga cccgagcgc gcgaaactc gcctacgct 
ctagggatg acggagtcc caggttaaa gtatggttt 
cagaacaga cgcaccaag tggcggaaa aagcacgcg 
gctgaaatg gcgacggcc aagcggaaa caagaagtg 
ctagacaac caaaccgac gagaacagt gacgtcatt 
tccgacact gaagatgaa cacgcagcg aagagacag 
aacatacaa tga
    • >TC014200 RB
      atgacgcaa cacagcgac gcgtccagc gaccgatcc 
gaagtctcc gtgacgccg ccaccgcca gtctccaaa 
atgctggag ttcgccagc cagcagaac ctcagcttc 
ctcaacatt gaacaccgg aacatgctc gccgccccg 
ctggcggcg ctccactcc atgacggag atgaagcaa 
caccaacac cagcaccac ctcagcccg gaccacctg 
cacggcccc gagaaagcc gacgatgtc ctgctggag 
cgtacaagt ttacacgta gaacgctcc ggcttgacc 
tcgcgcagc cccctgggg gccacgagc aacagcaat 
agctcccaa ggagccaac ccgcacggc atagaccac 
atactctcg cgaccctcg caaatcacg acgggcccg 
ctgcacttc ccgcacagc ctggggttc acgcagaac 
accctgggg atggcctcc aacagcgcg cagacggcc 
tcaaactcg ggcctacga gggttcaac atcgccgcc 
gctgccttc ttccatcac cacgccgcc aactccgtc 
aacaagccg cccgttgag ctcggaaga agcaccggc 
ggcctgtac tggcccggc ttccaggga ctcgtctcc 
aaccccatc gcttggcgg gatcggctc agcagcatg 
aatcagggc aatgtggga ggaattggg ccggacaag 
gacacgaaa aagaagcac acgaggcct actttctca 
ggacaacaa attttcgcc ctggaaaaa accttcgaa 
caaaccaaa tacttggca ggacccgag cgcgcgaaa 
ctcgcctac gctctaggg atgacggag tcccaggtt 
aaagtatgg tttcagaac agacgcacc aagtggcgg 
aaaaagcac gcggctgaa atggcgacg gccaagcgg 
aaacaagaa gtgctagac aaccaaacc gacgagaac 
agtgacgtc atttccgac actgaagat gaacacgca 
gcgaagaga cagaacata caatga
    mRNA sequence
    • >TC014200 RA
      cagtgctaa aagcctttt ggatattac atgttaaaa 
tggggattg agtctcatg tatactccg ataatacga 
gtccggagg gggaagggc ggtcttgca accgtgcgg 
gcggttcca gacgcgcgc gcgaccgcc aatcacggc 
gccgttctg ggcccgccg gtcctcctt cccgccaat 
gaaaaaagc attaatctc ttttaaaaa tagagcagt 
gtgcagcca cgcagccgg gcagcacgc accgccgtc 
cgaatctcc cctaccata aaggccagt gtaatgagc 
tttaacggt agcacatat ttaacatgc actacagtt 
catagacat ccccaccgc ggaggccgc tgagtcacc 
ttgccgccg cccaccgcc tcagtcagt gctcacctc 
aaagtctaa gtcagttca ttgccgacg ccgagtcac 
cgttggcca ccgcgaagt gctgtgatg tgcagtgct 
agccacgtg ttgtgagag ccctgacta gcccgcgac 
gcgaaacgc gtcctcacc gcaaccacg gcgtctccc 
gaagcaatg acgcaacac agcgacgcg tccagcgac 
cgatccgaa gtctccgtg acgccgcca ccgccagtc 
tccaaaatg ctggagttc gccagccag cagaacctc 
agcttcctc aacattgaa caccggaac atgctcgcc 
gccccgctg gcggcgctc cactccatg acggagatg 
aagcaacac caacaccag caccacctc agcccggac 
cacctgcac ggccccgag aaagccgac gatgtcctg 
ctggagcgt acaagttta cacgtagaa cgctccggc 
ttgacctcg cgcagcccc ctgggggcc acgagcaac 
agcaatagc tcccaagga gccaacccg cacggcata 
gaccacata ctctcgcga ccctcgcaa atcacgacg 
ggcccgctg cacttcccg cacagcctg gggttcacg 
cagaacacc ctggggatg gcctccaac agcgcgcag 
acggcctca aactcgggc ctacgaggg ttcaacatc 
gccgccgct gccttcttc catcaccac gccgccaac 
tccgtcaac aagccgccc gttgagctc ggaagaagc 
accggcggc ctgtactgg cccggcttc cagggactc 
gtctccaac cccatcgct tggcgggat cggctcagc 
agcaaacaa gatgcttac tttaaattt gtgaatcag 
ggcaatgtg ggaggaatt gggccggac aaggacacg 
aaaaagaag cacacgagg cctactttc tcaggacaa 
caaattttc gccctggaa aaaaccttc gaacaaacc 
aaatacttg gcaggaccc gagcgcgcg aaactcgcc 
tacgctcta gggatgacg gagtcccag gttaaagta 
tggtttcag aacagacgc accaagtgg cggaaaaag 
cacgcggct gaaatggcg acggccaag cggaaacaa 
gaagtgcta gacaaccaa accgacgag aacagtgac 
gtcatttcc gacactgaa gatgaacac gcagcgaag 
agacagaac atacaatga gaactcgaa ctcgttcaa 
atagagacc aagactctt gtaaatatg gtttagtga 
tctcagtgt ttgtgaaga cttgtgcgt gggacaagg 
gacgttgtg acgcgcttt gtaaaaatg ttatatatc 
tataaatat atgtatacg tattataac gctatttat 
tgttctttt ggcgcgcga aactgcgcg atttcgccg 
accagggga aattgggcc gttttcgcg ccgacttgt 
acagcagct tgaaacatt gcgggaata atatcctcc 
attgtacat aactttctt ttgcgtttg atagcaata 
tttacaggg tgattacgg aaataggct cgtggaaaa 
tattattcc cattaatat ttttgattt aagagctcg 
ccgctctca aatgagctg aatagaaat ttgtgttgg 
aaaattcaa aacatcgtg caatcttct tactatagg 
acgcggcat tttgcagct aaattatgt atattaaaa 
tgtatttgt aatattaaa ataaatatg ttcaagtga 
a
    • >TC014200 RB
      cagtgctaa aagcctttt ggatattac atgttaaaa 
tggggattg agtctcatg tatactccg ataatacga 
gtccggagg gggaagggc ggtcttgca accgtgcgg 
gcggttcca gacgcgcgc gcgaccgcc aatcacggc 
gccgttctg ggcccgccg gtcctcctt cccgccaat 
gaaaaaagc attaatctc ttttaaaaa tagagcagt 
gtgcagcca cgcagccgg gcagcacgc accgccgtc 
cgaatctcc cctaccata aaggccagt gtaatgagc 
tttaacggt agcacatat ttaacatgc actacagtt 
catagacat ccccaccgc ggaggccgc tgagtcacc 
ttgccgccg cccaccgcc tcagtcagt gctcacctc 
aaagtctaa gtcagttca ttgccgacg ccgagtcac 
cgttggcca ccgcgaagt gctgtgatg tgcagtgct 
agccacgtg ttgtgagag ccctgacta gcccgcgac 
gcgaaacgc gtcctcacc gcaaccacg gcgtctccc 
gaagcaatg acgcaacac agcgacgcg tccagcgac 
cgatccgaa gtctccgtg acgccgcca ccgccagtc 
tccaaaatg ctggagttc gccagccag cagaacctc 
agcttcctc aacattgaa caccggaac atgctcgcc 
gccccgctg gcggcgctc cactccatg acggagatg 
aagcaacac caacaccag caccacctc agcccggac 
cacctgcac ggccccgag aaagccgac gatgtcctg 
ctggagcgt acaagttta cacgtagaa cgctccggc 
ttgacctcg cgcagcccc ctgggggcc acgagcaac 
agcaatagc tcccaagga gccaacccg cacggcata 
gaccacata ctctcgcga ccctcgcaa atcacgacg 
ggcccgctg cacttcccg cacagcctg gggttcacg 
cagaacacc ctggggatg gcctccaac agcgcgcag 
acggcctca aactcgggc ctacgaggg ttcaacatc 
gccgccgct gccttcttc catcaccac gccgccaac 
tccgtcaac aagccgccc gttgagctc ggaagaagc 
accggcggc ctgtactgg cccggcttc cagggactc 
gtctccaac cccatcgct tggcgggat cggctcagc 
agcatgaat cagggcaat gtgggagga attgggccg 
gacaaggac acgaaaaag aagcacacg aggcctact 
ttctcagga caacaaatt ttcgccctg gaaaaaacc 
ttcgaacaa accaaatac ttggcagga cccgagcgc 
gcgaaactc gcctacgct ctagggatg acggagtcc 
caggttaaa gtatggttt cagaacaga cgcaccaag 
tggcggaaa aagcacgcg gctgaaatg gcgacggcc 
aagcggaaa caagaagtg ctagacaac caaaccgac 
gagaacagt gacgtcatt tccgacact gaagatgaa 
cacgcagcg aagagacag aacatacaa tgagaactc 
gaactcgtt caaatagag accaagact cttgtaaat 
atggtttag tgatctcag tgtttgtga agacttgtg 
cgtgggaca agggacgtt gtgacgcgc tttgtaaaa 
atgttatat atctataaa tatatgtat acgtattat 
aacgctatt tattgttct tttggcgcg cgaaactgc 
gcgatttcg ccgaccagg ggaaattgg gccgttttc 
gcgccgact tgtacagca gcttgaaac attgcggga 
ataatatcc tccattgta cataacttt cttttgcgt 
ttgatagca atatttaca gggtgatta cggaaatag 
gctcgtgga aaatattat tcccattaa tatttttga 
tttaagagc tcgccgctc tcaaatgag ctgaataga 
aatttgtgt tggaaaatt caaaacatc gtgcaatct 
tcttactat aggacgcgg cattttgca gctaaatta 
tgtatatta aaatgtatt tgtaatatt aaaataaat 
atgttcaag tgaa
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