Status
Please wait ...
Gene information
General information
TC014200 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3072788..3090417
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014200
    iBeetle screen iB_06579

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014200 RA
      MTQHSDASS DRSEVSVTP PPPVSKMLE FASQQNLSF 
      LNIEHRNML AAPLAALHS MTEMKQHQH QHHLSPDHL 
      HGPEKADDV LLERTSLHV ERSGLTSRS PLGATSNSN 
      SSQGANPHG IDHILSRPS QITTGPLHF PHSLGFTQN 
      TLGMASNSA QTASNSGLR GFNIAAAAF FHHHAANSV 
      NKPPVELGR STGGLYWPG FQGLVSNPI AWRDRLSSK 
      QDAYFKFVN QGNVGGIGP DKDTKKKHT RPTFSGQQI 
      FALEKTFEQ TKYLAGPER AKLAYALGM TESQVKVWF 
      QNRRTKWRK KHAAEMATA KRKQEVLDN QTDENSDVI 
      SDTEDEHAA KRQNIQ
    • >TC014200 RB
      MTQHSDASS DRSEVSVTP PPPVSKMLE FASQQNLSF 
      LNIEHRNML AAPLAALHS MTEMKQHQH QHHLSPDHL 
      HGPEKADDV LLERTSLHV ERSGLTSRS PLGATSNSN 
      SSQGANPHG IDHILSRPS QITTGPLHF PHSLGFTQN 
      TLGMASNSA QTASNSGLR GFNIAAAAF FHHHAANSV 
      NKPPVELGR STGGLYWPG FQGLVSNPI AWRDRLSSM 
      NQGNVGGIG PDKDTKKKH TRPTFSGQQ IFALEKTFE 
      QTKYLAGPE RAKLAYALG MTESQVKVW FQNRRTKWR 
      KKHAAEMAT AKRKQEVLD NQTDENSDV ISDTEDEHA 
      AKRQNIQ
    CDS sequence
    • >TC014200 RA
      atgacgcaa cacagcgac gcgtccagc gaccgatcc 
      gaagtctcc gtgacgccg ccaccgcca gtctccaaa 
      atgctggag ttcgccagc cagcagaac ctcagcttc 
      ctcaacatt gaacaccgg aacatgctc gccgccccg 
      ctggcggcg ctccactcc atgacggag atgaagcaa 
      caccaacac cagcaccac ctcagcccg gaccacctg 
      cacggcccc gagaaagcc gacgatgtc ctgctggag 
      cgtacaagt ttacacgta gaacgctcc ggcttgacc 
      tcgcgcagc cccctgggg gccacgagc aacagcaat 
      agctcccaa ggagccaac ccgcacggc atagaccac 
      atactctcg cgaccctcg caaatcacg acgggcccg 
      ctgcacttc ccgcacagc ctggggttc acgcagaac 
      accctgggg atggcctcc aacagcgcg cagacggcc 
      tcaaactcg ggcctacga gggttcaac atcgccgcc 
      gctgccttc ttccatcac cacgccgcc aactccgtc 
      aacaagccg cccgttgag ctcggaaga agcaccggc 
      ggcctgtac tggcccggc ttccaggga ctcgtctcc 
      aaccccatc gcttggcgg gatcggctc agcagcaaa 
      caagatgct tactttaaa tttgtgaat cagggcaat 
      gtgggagga attgggccg gacaaggac acgaaaaag 
      aagcacacg aggcctact ttctcagga caacaaatt 
      ttcgccctg gaaaaaacc ttcgaacaa accaaatac 
      ttggcagga cccgagcgc gcgaaactc gcctacgct 
      ctagggatg acggagtcc caggttaaa gtatggttt 
      cagaacaga cgcaccaag tggcggaaa aagcacgcg 
      gctgaaatg gcgacggcc aagcggaaa caagaagtg 
      ctagacaac caaaccgac gagaacagt gacgtcatt 
      tccgacact gaagatgaa cacgcagcg aagagacag 
      aacatacaa tga
    • >TC014200 RB
      atgacgcaa cacagcgac gcgtccagc gaccgatcc 
      gaagtctcc gtgacgccg ccaccgcca gtctccaaa 
      atgctggag ttcgccagc cagcagaac ctcagcttc 
      ctcaacatt gaacaccgg aacatgctc gccgccccg 
      ctggcggcg ctccactcc atgacggag atgaagcaa 
      caccaacac cagcaccac ctcagcccg gaccacctg 
      cacggcccc gagaaagcc gacgatgtc ctgctggag 
      cgtacaagt ttacacgta gaacgctcc ggcttgacc 
      tcgcgcagc cccctgggg gccacgagc aacagcaat 
      agctcccaa ggagccaac ccgcacggc atagaccac 
      atactctcg cgaccctcg caaatcacg acgggcccg 
      ctgcacttc ccgcacagc ctggggttc acgcagaac 
      accctgggg atggcctcc aacagcgcg cagacggcc 
      tcaaactcg ggcctacga gggttcaac atcgccgcc 
      gctgccttc ttccatcac cacgccgcc aactccgtc 
      aacaagccg cccgttgag ctcggaaga agcaccggc 
      ggcctgtac tggcccggc ttccaggga ctcgtctcc 
      aaccccatc gcttggcgg gatcggctc agcagcatg 
      aatcagggc aatgtggga ggaattggg ccggacaag 
      gacacgaaa aagaagcac acgaggcct actttctca 
      ggacaacaa attttcgcc ctggaaaaa accttcgaa 
      caaaccaaa tacttggca ggacccgag cgcgcgaaa 
      ctcgcctac gctctaggg atgacggag tcccaggtt 
      aaagtatgg tttcagaac agacgcacc aagtggcgg 
      aaaaagcac gcggctgaa atggcgacg gccaagcgg 
      aaacaagaa gtgctagac aaccaaacc gacgagaac 
      agtgacgtc atttccgac actgaagat gaacacgca 
      gcgaagaga cagaacata caatga
    mRNA sequence
    • >TC014200 RA
      cagtgctaa aagcctttt ggatattac atgttaaaa 
      tggggattg agtctcatg tatactccg ataatacga 
      gtccggagg gggaagggc ggtcttgca accgtgcgg 
      gcggttcca gacgcgcgc gcgaccgcc aatcacggc 
      gccgttctg ggcccgccg gtcctcctt cccgccaat 
      gaaaaaagc attaatctc ttttaaaaa tagagcagt 
      gtgcagcca cgcagccgg gcagcacgc accgccgtc 
      cgaatctcc cctaccata aaggccagt gtaatgagc 
      tttaacggt agcacatat ttaacatgc actacagtt 
      catagacat ccccaccgc ggaggccgc tgagtcacc 
      ttgccgccg cccaccgcc tcagtcagt gctcacctc 
      aaagtctaa gtcagttca ttgccgacg ccgagtcac 
      cgttggcca ccgcgaagt gctgtgatg tgcagtgct 
      agccacgtg ttgtgagag ccctgacta gcccgcgac 
      gcgaaacgc gtcctcacc gcaaccacg gcgtctccc 
      gaagcaatg acgcaacac agcgacgcg tccagcgac 
      cgatccgaa gtctccgtg acgccgcca ccgccagtc 
      tccaaaatg ctggagttc gccagccag cagaacctc 
      agcttcctc aacattgaa caccggaac atgctcgcc 
      gccccgctg gcggcgctc cactccatg acggagatg 
      aagcaacac caacaccag caccacctc agcccggac 
      cacctgcac ggccccgag aaagccgac gatgtcctg 
      ctggagcgt acaagttta cacgtagaa cgctccggc 
      ttgacctcg cgcagcccc ctgggggcc acgagcaac 
      agcaatagc tcccaagga gccaacccg cacggcata 
      gaccacata ctctcgcga ccctcgcaa atcacgacg 
      ggcccgctg cacttcccg cacagcctg gggttcacg 
      cagaacacc ctggggatg gcctccaac agcgcgcag 
      acggcctca aactcgggc ctacgaggg ttcaacatc 
      gccgccgct gccttcttc catcaccac gccgccaac 
      tccgtcaac aagccgccc gttgagctc ggaagaagc 
      accggcggc ctgtactgg cccggcttc cagggactc 
      gtctccaac cccatcgct tggcgggat cggctcagc 
      agcaaacaa gatgcttac tttaaattt gtgaatcag 
      ggcaatgtg ggaggaatt gggccggac aaggacacg 
      aaaaagaag cacacgagg cctactttc tcaggacaa 
      caaattttc gccctggaa aaaaccttc gaacaaacc 
      aaatacttg gcaggaccc gagcgcgcg aaactcgcc 
      tacgctcta gggatgacg gagtcccag gttaaagta 
      tggtttcag aacagacgc accaagtgg cggaaaaag 
      cacgcggct gaaatggcg acggccaag cggaaacaa 
      gaagtgcta gacaaccaa accgacgag aacagtgac 
      gtcatttcc gacactgaa gatgaacac gcagcgaag 
      agacagaac atacaatga gaactcgaa ctcgttcaa 
      atagagacc aagactctt gtaaatatg gtttagtga 
      tctcagtgt ttgtgaaga cttgtgcgt gggacaagg 
      gacgttgtg acgcgcttt gtaaaaatg ttatatatc 
      tataaatat atgtatacg tattataac gctatttat 
      tgttctttt ggcgcgcga aactgcgcg atttcgccg 
      accagggga aattgggcc gttttcgcg ccgacttgt 
      acagcagct tgaaacatt gcgggaata atatcctcc 
      attgtacat aactttctt ttgcgtttg atagcaata 
      tttacaggg tgattacgg aaataggct cgtggaaaa 
      tattattcc cattaatat ttttgattt aagagctcg 
      ccgctctca aatgagctg aatagaaat ttgtgttgg 
      aaaattcaa aacatcgtg caatcttct tactatagg 
      acgcggcat tttgcagct aaattatgt atattaaaa 
      tgtatttgt aatattaaa ataaatatg ttcaagtga 
      a
    • >TC014200 RB
      cagtgctaa aagcctttt ggatattac atgttaaaa 
      tggggattg agtctcatg tatactccg ataatacga 
      gtccggagg gggaagggc ggtcttgca accgtgcgg 
      gcggttcca gacgcgcgc gcgaccgcc aatcacggc 
      gccgttctg ggcccgccg gtcctcctt cccgccaat 
      gaaaaaagc attaatctc ttttaaaaa tagagcagt 
      gtgcagcca cgcagccgg gcagcacgc accgccgtc 
      cgaatctcc cctaccata aaggccagt gtaatgagc 
      tttaacggt agcacatat ttaacatgc actacagtt 
      catagacat ccccaccgc ggaggccgc tgagtcacc 
      ttgccgccg cccaccgcc tcagtcagt gctcacctc 
      aaagtctaa gtcagttca ttgccgacg ccgagtcac 
      cgttggcca ccgcgaagt gctgtgatg tgcagtgct 
      agccacgtg ttgtgagag ccctgacta gcccgcgac 
      gcgaaacgc gtcctcacc gcaaccacg gcgtctccc 
      gaagcaatg acgcaacac agcgacgcg tccagcgac 
      cgatccgaa gtctccgtg acgccgcca ccgccagtc 
      tccaaaatg ctggagttc gccagccag cagaacctc 
      agcttcctc aacattgaa caccggaac atgctcgcc 
      gccccgctg gcggcgctc cactccatg acggagatg 
      aagcaacac caacaccag caccacctc agcccggac 
      cacctgcac ggccccgag aaagccgac gatgtcctg 
      ctggagcgt acaagttta cacgtagaa cgctccggc 
      ttgacctcg cgcagcccc ctgggggcc acgagcaac 
      agcaatagc tcccaagga gccaacccg cacggcata 
      gaccacata ctctcgcga ccctcgcaa atcacgacg 
      ggcccgctg cacttcccg cacagcctg gggttcacg 
      cagaacacc ctggggatg gcctccaac agcgcgcag 
      acggcctca aactcgggc ctacgaggg ttcaacatc 
      gccgccgct gccttcttc catcaccac gccgccaac 
      tccgtcaac aagccgccc gttgagctc ggaagaagc 
      accggcggc ctgtactgg cccggcttc cagggactc 
      gtctccaac cccatcgct tggcgggat cggctcagc 
      agcatgaat cagggcaat gtgggagga attgggccg 
      gacaaggac acgaaaaag aagcacacg aggcctact 
      ttctcagga caacaaatt ttcgccctg gaaaaaacc 
      ttcgaacaa accaaatac ttggcagga cccgagcgc 
      gcgaaactc gcctacgct ctagggatg acggagtcc 
      caggttaaa gtatggttt cagaacaga cgcaccaag 
      tggcggaaa aagcacgcg gctgaaatg gcgacggcc 
      aagcggaaa caagaagtg ctagacaac caaaccgac 
      gagaacagt gacgtcatt tccgacact gaagatgaa 
      cacgcagcg aagagacag aacatacaa tgagaactc 
      gaactcgtt caaatagag accaagact cttgtaaat 
      atggtttag tgatctcag tgtttgtga agacttgtg 
      cgtgggaca agggacgtt gtgacgcgc tttgtaaaa 
      atgttatat atctataaa tatatgtat acgtattat 
      aacgctatt tattgttct tttggcgcg cgaaactgc 
      gcgatttcg ccgaccagg ggaaattgg gccgttttc 
      gcgccgact tgtacagca gcttgaaac attgcggga 
      ataatatcc tccattgta cataacttt cttttgcgt 
      ttgatagca atatttaca gggtgatta cggaaatag 
      gctcgtgga aaatattat tcccattaa tatttttga 
      tttaagagc tcgccgctc tcaaatgag ctgaataga 
      aatttgtgt tggaaaatt caaaacatc gtgcaatct 
      tcttactat aggacgcgg cattttgca gctaaatta 
      tgtatatta aaatgtatt tgtaatatt aaaataaat 
      atgttcaag tgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY