| General information | |||
| TC014200 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 3072788..3090417 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC014200 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06579 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC014200 RA
MTQHSDASS DRSEVSVTP PPPVSKMLE FASQQNLSF LNIEHRNML AAPLAALHS MTEMKQHQH QHHLSPDHL HGPEKADDV LLERTSLHV ERSGLTSRS PLGATSNSN SSQGANPHG IDHILSRPS QITTGPLHF PHSLGFTQN TLGMASNSA QTASNSGLR GFNIAAAAF FHHHAANSV NKPPVELGR STGGLYWPG FQGLVSNPI AWRDRLSSK QDAYFKFVN QGNVGGIGP DKDTKKKHT RPTFSGQQI FALEKTFEQ TKYLAGPER AKLAYALGM TESQVKVWF QNRRTKWRK KHAAEMATA KRKQEVLDN QTDENSDVI SDTEDEHAA KRQNIQ
- >TC014200 RB
MTQHSDASS DRSEVSVTP PPPVSKMLE FASQQNLSF LNIEHRNML AAPLAALHS MTEMKQHQH QHHLSPDHL HGPEKADDV LLERTSLHV ERSGLTSRS PLGATSNSN SSQGANPHG IDHILSRPS QITTGPLHF PHSLGFTQN TLGMASNSA QTASNSGLR GFNIAAAAF FHHHAANSV NKPPVELGR STGGLYWPG FQGLVSNPI AWRDRLSSM NQGNVGGIG PDKDTKKKH TRPTFSGQQ IFALEKTFE QTKYLAGPE RAKLAYALG MTESQVKVW FQNRRTKWR KKHAAEMAT AKRKQEVLD NQTDENSDV ISDTEDEHA AKRQNIQ
- >TC014200 RA
atgacgcaa cacagcgac gcgtccagc gaccgatcc gaagtctcc gtgacgccg ccaccgcca gtctccaaa atgctggag ttcgccagc cagcagaac ctcagcttc ctcaacatt gaacaccgg aacatgctc gccgccccg ctggcggcg ctccactcc atgacggag atgaagcaa caccaacac cagcaccac ctcagcccg gaccacctg cacggcccc gagaaagcc gacgatgtc ctgctggag cgtacaagt ttacacgta gaacgctcc ggcttgacc tcgcgcagc cccctgggg gccacgagc aacagcaat agctcccaa ggagccaac ccgcacggc atagaccac atactctcg cgaccctcg caaatcacg acgggcccg ctgcacttc ccgcacagc ctggggttc acgcagaac accctgggg atggcctcc aacagcgcg cagacggcc tcaaactcg ggcctacga gggttcaac atcgccgcc gctgccttc ttccatcac cacgccgcc aactccgtc aacaagccg cccgttgag ctcggaaga agcaccggc ggcctgtac tggcccggc ttccaggga ctcgtctcc aaccccatc gcttggcgg gatcggctc agcagcaaa caagatgct tactttaaa tttgtgaat cagggcaat gtgggagga attgggccg gacaaggac acgaaaaag aagcacacg aggcctact ttctcagga caacaaatt ttcgccctg gaaaaaacc ttcgaacaa accaaatac ttggcagga cccgagcgc gcgaaactc gcctacgct ctagggatg acggagtcc caggttaaa gtatggttt cagaacaga cgcaccaag tggcggaaa aagcacgcg gctgaaatg gcgacggcc aagcggaaa caagaagtg ctagacaac caaaccgac gagaacagt gacgtcatt tccgacact gaagatgaa cacgcagcg aagagacag aacatacaa tga
- >TC014200 RB
atgacgcaa cacagcgac gcgtccagc gaccgatcc gaagtctcc gtgacgccg ccaccgcca gtctccaaa atgctggag ttcgccagc cagcagaac ctcagcttc ctcaacatt gaacaccgg aacatgctc gccgccccg ctggcggcg ctccactcc atgacggag atgaagcaa caccaacac cagcaccac ctcagcccg gaccacctg cacggcccc gagaaagcc gacgatgtc ctgctggag cgtacaagt ttacacgta gaacgctcc ggcttgacc tcgcgcagc cccctgggg gccacgagc aacagcaat agctcccaa ggagccaac ccgcacggc atagaccac atactctcg cgaccctcg caaatcacg acgggcccg ctgcacttc ccgcacagc ctggggttc acgcagaac accctgggg atggcctcc aacagcgcg cagacggcc tcaaactcg ggcctacga gggttcaac atcgccgcc gctgccttc ttccatcac cacgccgcc aactccgtc aacaagccg cccgttgag ctcggaaga agcaccggc ggcctgtac tggcccggc ttccaggga ctcgtctcc aaccccatc gcttggcgg gatcggctc agcagcatg aatcagggc aatgtggga ggaattggg ccggacaag gacacgaaa aagaagcac acgaggcct actttctca ggacaacaa attttcgcc ctggaaaaa accttcgaa caaaccaaa tacttggca ggacccgag cgcgcgaaa ctcgcctac gctctaggg atgacggag tcccaggtt aaagtatgg tttcagaac agacgcacc aagtggcgg aaaaagcac gcggctgaa atggcgacg gccaagcgg aaacaagaa gtgctagac aaccaaacc gacgagaac agtgacgtc atttccgac actgaagat gaacacgca gcgaagaga cagaacata caatga
- >TC014200 RA
cagtgctaa aagcctttt ggatattac atgttaaaa tggggattg agtctcatg tatactccg ataatacga gtccggagg gggaagggc ggtcttgca accgtgcgg gcggttcca gacgcgcgc gcgaccgcc aatcacggc gccgttctg ggcccgccg gtcctcctt cccgccaat gaaaaaagc attaatctc ttttaaaaa tagagcagt gtgcagcca cgcagccgg gcagcacgc accgccgtc cgaatctcc cctaccata aaggccagt gtaatgagc tttaacggt agcacatat ttaacatgc actacagtt catagacat ccccaccgc ggaggccgc tgagtcacc ttgccgccg cccaccgcc tcagtcagt gctcacctc aaagtctaa gtcagttca ttgccgacg ccgagtcac cgttggcca ccgcgaagt gctgtgatg tgcagtgct agccacgtg ttgtgagag ccctgacta gcccgcgac gcgaaacgc gtcctcacc gcaaccacg gcgtctccc gaagcaatg acgcaacac agcgacgcg tccagcgac cgatccgaa gtctccgtg acgccgcca ccgccagtc tccaaaatg ctggagttc gccagccag cagaacctc agcttcctc aacattgaa caccggaac atgctcgcc gccccgctg gcggcgctc cactccatg acggagatg aagcaacac caacaccag caccacctc agcccggac cacctgcac ggccccgag aaagccgac gatgtcctg ctggagcgt acaagttta cacgtagaa cgctccggc ttgacctcg cgcagcccc ctgggggcc acgagcaac agcaatagc tcccaagga gccaacccg cacggcata gaccacata ctctcgcga ccctcgcaa atcacgacg ggcccgctg cacttcccg cacagcctg gggttcacg cagaacacc ctggggatg gcctccaac agcgcgcag acggcctca aactcgggc ctacgaggg ttcaacatc gccgccgct gccttcttc catcaccac gccgccaac tccgtcaac aagccgccc gttgagctc ggaagaagc accggcggc ctgtactgg cccggcttc cagggactc gtctccaac cccatcgct tggcgggat cggctcagc agcaaacaa gatgcttac tttaaattt gtgaatcag ggcaatgtg ggaggaatt gggccggac aaggacacg aaaaagaag cacacgagg cctactttc tcaggacaa caaattttc gccctggaa aaaaccttc gaacaaacc aaatacttg gcaggaccc gagcgcgcg aaactcgcc tacgctcta gggatgacg gagtcccag gttaaagta tggtttcag aacagacgc accaagtgg cggaaaaag cacgcggct gaaatggcg acggccaag cggaaacaa gaagtgcta gacaaccaa accgacgag aacagtgac gtcatttcc gacactgaa gatgaacac gcagcgaag agacagaac atacaatga gaactcgaa ctcgttcaa atagagacc aagactctt gtaaatatg gtttagtga tctcagtgt ttgtgaaga cttgtgcgt gggacaagg gacgttgtg acgcgcttt gtaaaaatg ttatatatc tataaatat atgtatacg tattataac gctatttat tgttctttt ggcgcgcga aactgcgcg atttcgccg accagggga aattgggcc gttttcgcg ccgacttgt acagcagct tgaaacatt gcgggaata atatcctcc attgtacat aactttctt ttgcgtttg atagcaata tttacaggg tgattacgg aaataggct cgtggaaaa tattattcc cattaatat ttttgattt aagagctcg ccgctctca aatgagctg aatagaaat ttgtgttgg aaaattcaa aacatcgtg caatcttct tactatagg acgcggcat tttgcagct aaattatgt atattaaaa tgtatttgt aatattaaa ataaatatg ttcaagtga a
- >TC014200 RB
cagtgctaa aagcctttt ggatattac atgttaaaa tggggattg agtctcatg tatactccg ataatacga gtccggagg gggaagggc ggtcttgca accgtgcgg gcggttcca gacgcgcgc gcgaccgcc aatcacggc gccgttctg ggcccgccg gtcctcctt cccgccaat gaaaaaagc attaatctc ttttaaaaa tagagcagt gtgcagcca cgcagccgg gcagcacgc accgccgtc cgaatctcc cctaccata aaggccagt gtaatgagc tttaacggt agcacatat ttaacatgc actacagtt catagacat ccccaccgc ggaggccgc tgagtcacc ttgccgccg cccaccgcc tcagtcagt gctcacctc aaagtctaa gtcagttca ttgccgacg ccgagtcac cgttggcca ccgcgaagt gctgtgatg tgcagtgct agccacgtg ttgtgagag ccctgacta gcccgcgac gcgaaacgc gtcctcacc gcaaccacg gcgtctccc gaagcaatg acgcaacac agcgacgcg tccagcgac cgatccgaa gtctccgtg acgccgcca ccgccagtc tccaaaatg ctggagttc gccagccag cagaacctc agcttcctc aacattgaa caccggaac atgctcgcc gccccgctg gcggcgctc cactccatg acggagatg aagcaacac caacaccag caccacctc agcccggac cacctgcac ggccccgag aaagccgac gatgtcctg ctggagcgt acaagttta cacgtagaa cgctccggc ttgacctcg cgcagcccc ctgggggcc acgagcaac agcaatagc tcccaagga gccaacccg cacggcata gaccacata ctctcgcga ccctcgcaa atcacgacg ggcccgctg cacttcccg cacagcctg gggttcacg cagaacacc ctggggatg gcctccaac agcgcgcag acggcctca aactcgggc ctacgaggg ttcaacatc gccgccgct gccttcttc catcaccac gccgccaac tccgtcaac aagccgccc gttgagctc ggaagaagc accggcggc ctgtactgg cccggcttc cagggactc gtctccaac cccatcgct tggcgggat cggctcagc agcatgaat cagggcaat gtgggagga attgggccg gacaaggac acgaaaaag aagcacacg aggcctact ttctcagga caacaaatt ttcgccctg gaaaaaacc ttcgaacaa accaaatac ttggcagga cccgagcgc gcgaaactc gcctacgct ctagggatg acggagtcc caggttaaa gtatggttt cagaacaga cgcaccaag tggcggaaa aagcacgcg gctgaaatg gcgacggcc aagcggaaa caagaagtg ctagacaac caaaccgac gagaacagt gacgtcatt tccgacact gaagatgaa cacgcagcg aagagacag aacatacaa tgagaactc gaactcgtt caaatagag accaagact cttgtaaat atggtttag tgatctcag tgtttgtga agacttgtg cgtgggaca agggacgtt gtgacgcgc tttgtaaaa atgttatat atctataaa tatatgtat acgtattat aacgctatt tattgttct tttggcgcg cgaaactgc gcgatttcg ccgaccagg ggaaattgg gccgttttc gcgccgact tgtacagca gcttgaaac attgcggga ataatatcc tccattgta cataacttt cttttgcgt ttgatagca atatttaca gggtgatta cggaaatag gctcgtgga aaatattat tcccattaa tatttttga tttaagagc tcgccgctc tcaaatgag ctgaataga aatttgtgt tggaaaatt caaaacatc gtgcaatct tcttactat aggacgcgg cattttgca gctaaatta tgtatatta aaatgtatt tgtaatatt aaaataaat atgttcaag tgaa
