Status
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Gene information
General information
TC013617 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3039201..3043076
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013617
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06577

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013617 RA
      MFKIFSIVL LAIAANCQL LRDEERPTR YVLPYSGLS 
      GGEEPEAAE EKNYYKPNR RSERIPATS YQYDDAEEE 
      YYRPQPQSR QKAESDSYS SRYQPSHKD YEEILKNNH 
      LVRLRVAAK KQSQPSQSQ PQQPVEQYL KEVNPTTKT 
      AYQRPLNYL RFNSQSALK QQEQSEETP LKQQSYRYL 
      LQPEPQQQK SYQQYQYES QQDSIKPVQ QKTPHFNLV 
      AYQNALIAQ QKLLAAAEN SQEENQSKS SLYVPQNVK 
      KAKKQKTTP SRQSSQQYD YYPQVPPQR PNYTPESPR 
      YPQSLPSRN AYQKSEANY QVEDPLLYP EPKELEYQT 
      QAPARSSKQ RTTRLPRRK PKYQTEEPE EYQPASDDQ 
      GFDYQPSSL KYQDEPTYR RPLPRPSKP AKYEEPSEA 
      KDDYLYQPL PKYQQNTPR RPQYSPEAG RYLSRQQYR 
      DESKQLPQY QAADLAYQP TAEVAVRQV GRGPSVSYR 
      QTYPQYQYD RRQQIATPL PKTRYFNSL EYQNEEDSS 
      DYITQKMRP STQYKYTRQ SNTLKLAPV RSYEVEEAS 
      EETVIVPQY QKEQGKYRP ENVIKKKGL HYPRRKKPT 
      YLNGQMFRE EVKKPRYYQ LEDLVTQPS SKTTLRYSP 
      NQPFLIVVY DD
    CDS sequence
    • >TC013617 RA
      atgtttaaa atattttca atagttctt ttggcgatt 
      gccgccaac tgtcaactg ttgagagac gaggagcga 
      ccgacgaga tacgtcctc ccgtactcc ggcttgtcc 
      ggaggcgaa gaaccggaa gcagcggaa gaaaaaaac 
      tattacaaa ccgaacaga cggtctgaa cgaattccg 
      gccacgtcg taccagtac gacgacgcc gaggaagag 
      tactacaga ccccagccc cagtcgcgc caaaaggcc 
      gaaagcgac tcctattcc agccgatac caaccgtcg 
      cacaaagac tacgaagaa attttgaaa aataatcac 
      ttagtgagg ctgagggtc gccgctaag aagcaatca 
      cagccttcg caatcgcag ccgcaacag cctgtcgag 
      cagtacttg aaagaagtt aatccgacg accaaaact 
      gcctatcag agacctttg aactatttg cggttcaat 
      agccagagc gctttgaag caacaagaa caaagtgaa 
      gaaacccct ttgaagcaa cagtcgtac cgatatctt 
      ttgcaacct gaaccgcaa caacagaaa tcgtaccaa 
      caatatcaa tacgaaagt caacaagat tctattaag 
      ccagttcag cagaaaaca ccccatttt aacttggtc 
      gcttatcaa aacgcccta atcgcccaa caaaaattg 
      ctagcagcg gctgaaaac agccaagaa gaaaaccag 
      tccaaatcc tcactttac gtgccgcaa aacgtgaaa 
      aaagccaaa aaacaaaaa acaacccct tcgcgtcag 
      tcgagccaa cagtacgat tactaccct caagtcccc 
      cctcaaaga cccaactac acccccgaa agcccccga 
      tacccacaa tcactgccc tcgcgaaat gcttaccaa 
      aagagtgaa gctaattac caagtcgaa gaccctctc 
      ttgtacccc gaacccaaa gagctggag taccaaact 
      caagcacct gccaggtct tcgaaacag agaactacc 
      agacttccg aggagaaaa ccaaagtac caaactgag 
      gaaccggag gagtaccag cccgcgtcc gacgatcag 
      ggattcgac taccaacca agctcgctg aagtaccaa 
      gacgaaccc acgtacaga cgccccttg cccaggccc 
      tcgaaacca gcaaagtac gaggaacca tcggaggcg 
      aaagacgat tacttgtac cagcctttg cccaagtac 
      cagcaaaac acaccgagg agaccgcag tactccccg 
      gaagccggc agatacctc agccggcag cagtaccga 
      gacgaaagc aaacaactc ccacaatac caagccgca 
      gatttggcg taccagccc accgccgaa gtcgcagtt 
      agacaagtt ggacgcgga ccgtctgtt tcttacagg 
      caaacatac ccacaatat cagtatgac agacggcaa 
      caaatcgcc acaccgctg ccaaaaact cgctacttc 
      aactcactc gagtaccaa aacgaagaa gactcttct 
      gattacatc acacaaaaa atgcgacca tccacacaa 
      tacaaatac actcgacaa tctaacact cttaaacta 
      gctcctgta cgaagctac gaagtcgag gaggcctcg 
      gaagagacc gtcatcgtg ccgcagtac cagaaagaa 
      caaggcaaa taccggccg gaaaacgtc atcaagaag 
      aaaggcctc cactacccg agaagaaag aaaccgact 
      tatctcaac gggcaaatg ttccgcgaa gaagtcaaa 
      aaaccgcgc tactaccag ctggaggac ctggtcacg 
      caaccctcc tccaaaacc acgcttcgc tacagccct 
      aaccaaccc ttcttaatc gtggtgtat gacgattaa
    mRNA sequence
    • >TC013617 RA
      tcgcaaacc tcagttctt ctttttgaa aatgtttaa 
      aatattttc aatagttct tttggcgat tgccgccaa 
      ctgtcaact gttgagaga cgaggagcg accgacgag 
      atacgtcct cccgtactc cggcttgtc cggaggcga 
      agaaccgga agcagcgga agaaaaaaa ctattacaa 
      accgaacag acggtctga acgaattcc ggccacgtc 
      gtaccagta cgacgacgc cgaggaaga gtactacag 
      accccagcc ccagtcgcg ccaaaaggc cgaaagcga 
      ctcctattc cagccgata ccaaccgtc gcacaaaga 
      ctacgaaga aattttgaa aaataatca cttagtgag 
      gctgagggt cgccgctaa gaagcaatc acagccttc 
      gcaatcgca gccgcaaca gcctgtcga gcagtactt 
      gaaagaagt taatccgac gaccaaaac tgcctatca 
      gagaccttt gaactattt gcggttcaa tagccagag 
      cgctttgaa gcaacaaga acaaagtga agaaacccc 
      tttgaagca acagtcgta ccgatatct tttgcaacc 
      tgaaccgca acaacagaa atcgtacca acaatatca 
      atacgaaag tcaacaaga ttctattaa gccagttca 
      gcagaaaac accccattt taacttggt cgcttatca 
      aaacgccct aatcgccca acaaaaatt gctagcagc 
      ggctgaaaa cagccaaga agaaaacca gtccaaatc 
      ctcacttta cgtgccgca aaacgtgaa aaaagccaa 
      aaaacaaaa aacaacccc ttcgcgtca gtcgagcca 
      acagtacga ttactaccc tcaagtccc ccctcaaag 
      acccaacta cacccccga aagcccccg atacccaca 
      atcactgcc ctcgcgaaa tgcttacca aaagagtga 
      agctaatta ccaagtcga agaccctct cttgtaccc 
      cgaacccaa agagctgga gtaccaaac tcaagcacc 
      tgccaggtc ttcgaaaca gagaactac cagacttcc 
      gaggagaaa accaaagta ccaaactga ggaaccgga 
      ggagtacca gcccgcgtc cgacgatca gggattcga 
      ctaccaacc aagctcgct gaagtacca agacgaacc 
      cacgtacag acgcccctt gcccaggcc ctcgaaacc 
      agcaaagta cgaggaacc atcggaggc gaaagacga 
      ttacttgta ccagccttt gcccaagta ccagcaaaa 
      cacaccgag gagaccgca gtactcccc ggaagccgg 
      cagatacct cagccggca gcagtaccg agacgaaag 
      caaacaact cccacaata ccaagccgc agatttggc 
      gtaccagcc caccgccga agtcgcagt tagacaagt 
      tggacgcgg accgtctgt ttcttacag gcaaacata 
      cccacaata tcagtatga cagacggca acaaatcgc 
      cacaccgct gccaaaaac tcgctactt caactcact 
      cgagtacca aaacgaaga agactcttc tgattacat 
      cacacaaaa aatgcgacc atccacaca atacaaata 
      cactcgaca atctaacac tcttaaact agctcctgt 
      acgaagcta cgaagtcga ggaggcctc ggaagagac 
      cgtcatcgt gccgcagta ccagaaaga acaaggcaa 
      ataccggcc ggaaaacgt catcaagaa gaaaggcct 
      ccactaccc gagaagaaa gaaaccgac ttatctcaa 
      cgggcaaat gttccgcga agaagtcaa aaaaccgcg 
      ctactacca gctggagga cctggtcac gcaaccctc 
      ctccaaaac cacgcttcg ctacagccc taaccaacc 
      cttcttaat cgtggtgta tgacgatta agtttttat 
      tgcctagtg tcttagaat tatccccat gctcaagta 
      ttaccgggg agtagtatt attgtgatg tttattcac 
      cttttaata tatttttat tacgtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY