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Gene information
General information
TC034518 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2959850..2970184
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034518
    iBeetle screen iB_06575

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034518 RA
      MLIAIPKEF RKYSKNCQS LRRILRDTC CALDDISSN 
      LQLGKEIKE DLLNQDVID KLHNKLDTV PTLMVFGQN 
      CHAKALFVN NLLDQIILP LFSNQWRYI TFKYGHSKT 
      FKLKAHEKP WVTIPEEDL QRIGDDLCA SLEVEICHT 
      LLKDNANIM VPPDCQPDQ VHEILNKHM KSVLPVIIY 
      AVSEDILSD SNIQEIRKF KELYNLPFL FVSVSNIGP 
      SQSTESLTE SEQHLLECN GGQKTLSKM QNLRDQLVR 
      LGYLNDSDN LKTEELMKR KSYCLDCSL YNTLIHDTQ 
      MNEELIVFI HEVLKSSLL KMAALLSEI HSQCLRRFI 
      LSAFDMARE IQITPKRIL YAQDIELKL YKKLMKIAG 
      EQQEEITGI IQRTLQEMK TNVSDVLEG YNKNDDHQS 
      TKMATLEIQ QLVLRRLRN SVATQIVQS VGCLQESFT 
      GTLQRCLES LEKNCHDLE GNLSASDAV KQILSAAYN 
      IDLKSSTSF SVVHTFMDR LRTLLSNFI LPWSSSQVQ 
      CNVQWQLQH PRIVKLRGS VIDYTYGGG SSPAVLLIM 
      ERMTRDLHC GLRNGLSWV VRLRIAIDV VEGIRYLHS 
      QGLVHRDIK LKNVLLDGE DRAKLTDFG FCIPEAMMS 
      GSVVGTPVH MAPELLSGR YDSSVDVYA FGILFWYIC 
      AGQVKLPNH FDQFQNKEQ LWNSVRKGI RPECLAHFN 
      QACWNLMEQ CWAAEPADR PLLGYVQPQ LEAIYNSAK 
      AESSDSYS
    CDS sequence
    • >TC034518 RA
      atgttgata gccatacca aaggaattc agaaaatat 
      tcgaaaaat tgtcaaagt ttaagacgg attttgagg 
      gatacatgc tgtgctcta gatgatatt agtagtaat 
      ttgcaatta ggaaaagaa atcaaggag gatttgcta 
      aatcaagat gtaattgat aagctgcat aacaaactt 
      gatacggta cccactctc atggttttc gggcaaaac 
      tgtcacgcc aaagcctta tttgtcaac aatttgttg 
      gatcagatc attttgccg ctctttagc aaccagtgg 
      cgttatata acttttaaa tatggacac tcaaaaaca 
      ttcaagcta aaagcgcat gaaaagccc tgggtcaca 
      atacccgag gaagacctg cagcggatt ggtgacgac 
      ctatgtgcg tccctagag gttgaaatc tgccacacg 
      ttactcaaa gataacgcc aatattatg gtcccaccc 
      gactgtcag ccggaccaa gtccatgaa attctaaac 
      aaacacatg aaaagtgtt ctgcctgtg ataatatat 
      gctgtgtcc gaggatatt ctcagtgat tctaatatt 
      caagaaatt cgtaagttc aaagagctc tacaatctg 
      ccgtttttg tttgtgagc gtttccaac attggacct 
      tcgcaaagc acggaatct ctaacagag tccgaacag 
      cacctgttg gagtgcaat ggaggacag aagactttg 
      agtaaaatg cagaatttg cgcgaccag ctcgtacga 
      ttaggctat ttgaatgac tcagacaac ttaaagact 
      gaagaactg atgaaacgg aaatcgtac tgtctggac 
      tgttcattg tacaatact ctgatccac gatacgcaa 
      atgaatgag gaactaata gtattcatt cacgaggtt 
      ttaaagtcg agtctctta aaaatggct gccttactt 
      agtgagatt catagtcag tgtttgcgg cgttttatt 
      ctgtccgcc ttcgacatg gcccgcgaa atccagata 
      actcccaaa cgtatatta tatgcccaa gatatcgag 
      ctgaaactt tacaagaag ctgatgaaa atagccggg 
      gagcagcag gaggaaatc accggaata atccagcga 
      acgttgcag gaaatgaag acgaacgtc agcgacgtc 
      ttggaagga tacaacaaa aacgatgac caccagtcg 
      acgaaaatg gcgactctg gaaatccag cagttggtg 
      ttgcggagg ctccggaac tccgtcgcg acgcaaatt 
      gtccagtcg gtcggttgt ttgcaggag agcttcacg 
      gggactttg cagcggtgt ttggaaagt ttggagaaa 
      aactgccat gatctcgaa ggaaatctc tcagcgtcg 
      gacgccgtg aagcagatc ttgagcgcg gcctataac 
      attgatttg aaatcgtcg acgtcgttt tccgttgtt 
      cacacgttc atggatcgg ctacgaacg ctcttatcg 
      aattttatc ctgccgtgg tcgtcgtca caagtccag 
      tgcaacgtc cagtggcaa ctgcagcat ccccgaatt 
      gtgaaactg cggggctct gtgattgat tacacgtat 
      gggggcggg agctcccct gctgtcctc ctgatcatg 
      gagaggatg actcgggac ttgcactgt ggccttcgt 
      aacggcctc tcttgggtg gtacgtctg cggattgcg 
      attgatgtt gttgaagga atccgctat ttgcactcc 
      caaggtctc gtccatcga gacatcaag ttgaaaaat 
      gtcctgctt gatggtgaa gatcgcgcg aagttgact 
      gatttcggt ttctgtatc cctgaagcc atgatgtcg 
      ggaagtgtc gttgggact cccgtgcac atggcccct 
      gaattattg tccggccgt tatgattcc agcgttgac 
      gtttatgct tttggaata ttgttttgg tacatatgt 
      gcagggcaa gtgaagttg ccgaatcat tttgatcag 
      tttcaaaat aaggaacag ctgtggaac agtgtgcgg 
      aaagggatt cggcctgaa tgtttagca cacttcaac 
      caagcgtgc tggaatctc atggaacag tgttgggcc 
      gcggaaccc gccgacaga ccgctgctt ggatatgta 
      cagccgcaa ctcgaagct atttataat agtgctaaa 
      gctgaatct tcagattcc tactcatga
    mRNA sequence
    • >TC034518 RA
      gtcatttga cattttgtt ttgacaagt ttatgtatg 
      gtttaattg taaaaatcc gattttaat attaggcat 
      gacagatta acagatcag tattttatt aattcaaat 
      atcaatgat ttaagtgcg caaaaatgt tgatagcca 
      taccaaagg aattcagaa aatattcga aaaattgtc 
      aaagtttaa gacggattt tgagggata catgctgtg 
      ctctagatg atattagta gtaatttgc aattaggaa 
      aagaaatca aggaggatt tgctaaatc aagatgtaa 
      ttgataagc tgcataaca aacttgata cggtaccca 
      ctctcatgg ttttcgggc aaaactgtc acgccaaag 
      ccttatttg tcaacaatt tgttggatc agatcattt 
      tgccgctct ttagcaacc agtggcgtt atataactt 
      ttaaatatg gacactcaa aaacattca agctaaaag 
      cgcatgaaa agccctggg tcacaatac ccgaggaag 
      acctgcagc ggattggtg acgacctat gtgcgtccc 
      tagaggttg aaatctgcc acacgttac tcaaagata 
      acgccaata ttatggtcc cacccgact gtcagccgg 
      accaagtcc atgaaattc taaacaaac acatgaaaa 
      gtgttctgc ctgtgataa tatatgctg tgtccgagg 
      atattctca gtgattcta atattcaag aaattcgta 
      agttcaaag agctctaca atctgccgt ttttgtttg 
      tgagcgttt ccaacattg gaccttcgc aaagcacgg 
      aatctctaa cagagtccg aacagcacc tgttggagt 
      gcaatggag gacagaaga ctttgagta aaatgcaga 
      atttgcgcg accagctcg tacgattag gctatttga 
      atgactcag acaacttaa agactgaag aactgatga 
      aacggaaat cgtactgtc tggactgtt cattgtaca 
      atactctga tccacgata cgcaaatga atgaggaac 
      taatagtat tcattcacg aggttttaa agtcgagtc 
      tcttaaaaa tggctgcct tacttagtg agattcata 
      gtcagtgtt tgcggcgtt ttattctgt ccgccttcg 
      acatggccc gcgaaatcc agataactc ccaaacgta 
      tattatatg cccaagata tcgagctga aactttaca 
      agaagctga tgaaaatag ccggggagc agcaggagg 
      aaatcaccg gaataatcc agcgaacgt tgcaggaaa 
      tgaagacga acgtcagcg acgtcttgg aaggataca 
      acaaaaacg atgaccacc agtcgacga aaatggcga 
      ctctggaaa tccagcagt tggtgttgc ggaggctcc 
      ggaactccg tcgcgacgc aaattgtcc agtcggtcg 
      gttgtttgc aggagagct tcacgggga ctttgcagc 
      ggtgtttgg aaagtttgg agaaaaact gccatgatc 
      tcgaaggaa atctctcag cgtcggacg ccgtgaagc 
      agatcttga gcgcggcct ataacattg atttgaaat 
      cgtcgacgt cgttttccg ttgttcaca cgttcatgg 
      atcggctac gaacgctct tatcgaatt ttatcctgc 
      cgtggtcgt cgtcacaag tccagtgca acgtccagt 
      ggcaactgc agcatcccc gaattgtga aactgcggg 
      gctctgtga ttgattaca cgtatgggg gcgggagct 
      cccctgctg tcctcctga tcatggaga ggatgactc 
      gggacttgc actgtggcc ttcgtaacg gcctctctt 
      gggtggtac gtctgcgga ttgcgattg atgttgttg 
      aaggaatcc gctatttgc actcccaag gtctcgtcc 
      atcgagaca tcaagttga aaaatgtcc tgcttgatg 
      gtgaagatc gcgcgaagt tgactgatt tcggtttct 
      gtatccctg aagccatga tgtcgggaa gtgtcgttg 
      ggactcccg tgcacatgg cccctgaat tattgtccg 
      gccgttatg attccagcg ttgacgttt atgcttttg 
      gaatattgt tttggtaca tatgtgcag ggcaagtga 
      agttgccga atcattttg atcagtttc aaaataagg 
      aacagctgt ggaacagtg tgcggaaag ggattcggc 
      ctgaatgtt tagcacact tcaaccaag cgtgctgga 
      atctcatgg aacagtgtt gggccgcgg aacccgccg 
      acagaccgc tgcttggat atgtacagc cgcaactcg 
      aagctattt ataatagtg ctaaagctg aatcttcag 
      attcctact catgaagag gatatttga aatgaaatt 
      acttttatc aaattaaaa cttactgta tata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY