Status
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Gene information
General information
TC030731 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9140211..9146659
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030731
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06574

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially rounded - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030731 RA
      MDTFLAKAY CQEGGGYLR RANPQHLHD IFNKYASQE 
      KNGERFMTP EDFIIKYLG LFEPENFNA HSVRLLAGI 
      VDTSKDGLI SYSEFQAFE GLLCFPDAL YKTAFQLFD 
      TNGNGMVSF QEFVEVMKK TELHERIPF NMDSPFIQL 
      YFGRDKKRL VPYSEFSQF LHDFHEEYA IEGFRRADK 
      TGSGFISVL DFQFIMTNI KSHLLTKQV QSHLIETAQ 
      GATSGRRVS FPYFIAFNS LLNNMELAK RIYLNVTNG 
      HRTQEVTKE EFMHSAQAM SQMTPLEVD ILFQLCDVL 
      HQTGRIVYN DLNAIAPEQ YYKQITNRL AEIHAVSSP 
      EDRGVFIQV LESMYRFTL GSVAGAVGA TAVYPIDLV 
      KTRMQNQRT GSFIGELMY RNSIDCFKK VIRHEGVFG 
      LYRGLVPQL MGVAPEKAI KLTVNDFVR DKFYDKNGN 
      ISGIGEVIS GAAAGASQV IFTNPLEIV KIRLQVAGE 
      IAGGSKVRA WHVVKELGL FGLYKGAKA CLLRDIPFS 
      AIYFPTYAH TKAKFADET GYNHPLSLL AAGAIAGVP 
      AAGLVTPAD VIKTRLQVV ARAGQTTYN GVFDAARKI 
      YVEEGFRAF WKGAIARVC RSSPQFGVT LVTYEVLQR 
      MLYIDFGGT RPAGSELKV TALGGDAPK LQKPDHVGG 
      YSVSIPVFS GIETKFGLC LPKLGLLSE RK
    CDS sequence
    • >TC030731 RA
      atggacaca tttttagct aaagcctat tgccaagag 
      ggtggtggg tacttgcga agagccaat ccgcagcat 
      ttacacgat attttcaac aagtatgcg tcgcaagaa 
      aagaacggg gagagattt atgaccccg gaggatttc 
      attattaaa tatctcggc ttgttcgaa ccggaaaat 
      tttaatgct cattcggtg cgtcttctc gccggaatt 
      gtcgacacg agcaaggat ggactcatt tcctactcg 
      gagttccaa gcttttgaa ggactcttg tgcttcccg 
      gacgcgctc tataaaacg gcatttcaa ttgttcgac 
      actaatgga aatggaatg gttagtttt caggaattc 
      gttgaggtg atgaagaaa actgagctc catgaacgc 
      atccctttc aacatggat tcgcccttt atccagctg 
      tacttcggg cgcgataag aaacgccta gtgccctac 
      agcgagttc agtcagttt ctgcacgat ttccacgaa 
      gagtacgca attgaaggc ttccgaagg gctgataag 
      acgggcagt gggttcatt tcggtgctg gattttcag 
      ttcatcatg accaatatc aaaagccat ttgctcact 
      aagcaagta caatctcac ctgatcgag acggctcaa 
      ggggcaacc agtggtcga agggtcagc tttccctat 
      ttcattgca tttaattcg ttgttaaac aacatggag 
      ttggcaaag cgtatttat ttgaatgtg acaaacggg 
      cacaggacg caggaagtg acgaaggaa gagttcatg 
      cattcggca caggcgatg tcgcaaatg acgcccctc 
      gaagtcgat attttgttt caactatgt gatgtactt 
      caccaaacg gggcggatc gtttataat gaccttaat 
      gccatcgca ccggaacaa tattataaa caaatcacg 
      aaccggctc gccgaaata cacgccgtc tctagtccc 
      gaagatcga ggagttttt atacaagtc ttggaaagc 
      atgtacagg tttacgcta ggctcagtg gcaggagcg 
      gttggcgct actgccgtt taccccatc gacttggta 
      aaaacccgt atgcagaac cagcgaacg ggctcattc 
      attggcgaa ttaatgtac agaaatagt atcgattgt 
      ttcaagaaa gtgatacgc catgagggc gtttttggt 
      ctctatcgt ggtctcgta ccacaatta atgggcgta 
      gcgcccgaa aaagccatc aaactgacg gtgaacgat 
      ttcgtgcgg gataaattc tacgacaaa aacggcaac 
      atctcgggg attggggag gtgatctcg ggggcagcg 
      gccggagct tcgcaagta attttcaca aatccgctg 
      gaaatcgtc aaaatccgg ttgcaagtc gccggcgaa 
      atcgccggc ggttcgaag gtcagggcg tggcacgtg 
      gtcaaagag ttggggctt tttgggctc tacaagggc 
      gcgaaggcc tgtctgctg cgggatatc cccttcagt 
      gcgatttac ttcccgact tacgctcac acgaaggcg 
      aaattcgcc gacgagacc ggttataac caccctttg 
      tccttgcta gcggctggg gctatcgcc ggtgtgccg 
      gctgccggg ctggtgacc ccagccgac gtcatcaag 
      acccggctc caggtggtg gcaagggcc gggcagacc 
      acctacaac ggggtgttt gacgccgcc aggaagatc 
      tacgtcgag gaagggttt agggccttt tggaagggc 
      gccattgcg agagtgtgt cggtcgtcc ccgcagttt 
      ggtgtgacg ctggtgact tacgaggtt ttgcaaaga 
      atgttatat attgatttc ggcggaacg agacccgct 
      gggtccgaa ttgaaggtc acggcgttg ggtggggac 
      gcccccaag ttgcagaag ccggaccat gtcgggggt 
      tactcagtc tccatacca gtcttctca ggaattgaa 
      actaagttt ggtctttgc ctgccaaaa cttggattg 
      ctttcggaa aggaagtga
    mRNA sequence
    • >TC030731 RA
      gacctcgtg ttatcaatc aatgtcgtt tctgtcaaa 
      tgctaaccc tggtgcaga gtttttgtg ggtttattc 
      taaatcagg ctgttaaaa aaattaatt aaacattag 
      ggacgtttt tttgttaaa caatttgtg ataattttt 
      tatgagatt tttgtgttt tgggaatgg acacatttt 
      tagctaaag cctattgcc aagagggtg gtgggtact 
      tgcgaagag ccaatccgc agcatttac acgatattt 
      tcaacaagt atgcgtcgc aagaaaaga acggggaga 
      gatttatga ccccggagg atttcatta ttaaatatc 
      tcggcttgt tcgaaccgg aaaatttta atgctcatt 
      cggtgcgtc ttctcgccg gaattgtcg acacgagca 
      aggatggac tcatttcct actcggagt tccaagctt 
      ttgaaggac tcttgtgct tcccggacg cgctctata 
      aaacggcat ttcaattgt tcgacacta atggaaatg 
      gaatggtta gttttcagg aattcgttg aggtgatga 
      agaaaactg agctccatg aacgcatcc ctttcaaca 
      tggattcgc cctttatcc agctgtact tcgggcgcg 
      ataagaaac gcctagtgc cctacagcg agttcagtc 
      agtttctgc acgatttcc acgaagagt acgcaattg 
      aaggcttcc gaagggctg ataagacgg gcagtgggt 
      tcatttcgg tgctggatt ttcagttca tcatgacca 
      atatcaaaa gccatttgc tcactaagc aagtacaat 
      ctcacctga tcgagacgg ctcaagggg caaccagtg 
      gtcgaaggg tcagctttc cctatttca ttgcattta 
      attcgttgt taaacaaca tggagttgg caaagcgta 
      tttatttga atgtgacaa acgggcaca ggacgcagg 
      aagtgacga aggaagagt tcatgcatt cggcacagg 
      cgatgtcgc aaatgacgc ccctcgaag tcgatattt 
      tgtttcaac tatgtgatg tacttcacc aaacggggc 
      ggatcgttt ataatgacc ttaatgcca tcgcaccgg 
      aacaatatt ataaacaaa tcacgaacc ggctcgccg 
      aaatacacg ccgtctcta gtcccgaag atcgaggag 
      tttttatac aagtcttgg aaagcatgt acaggttta 
      cgctaggct cagtggcag gagcggttg gcgctactg 
      ccgtttacc ccatcgact tggtaaaaa cccgtatgc 
      agaaccagc gaacgggct cattcattg gcgaattaa 
      tgtacagaa atagtatcg attgtttca agaaagtga 
      tacgccatg agggcgttt ttggtctct atcgtggtc 
      tcgtaccac aattaatgg gcgtagcgc ccgaaaaag 
      ccatcaaac tgacggtga acgatttcg tgcgggata 
      aattctacg acaaaaacg gcaacatct cggggattg 
      gggaggtga tctcggggg cagcggccg gagcttcgc 
      aagtaattt tcacaaatc cgctggaaa tcgtcaaaa 
      tccggttgc aagtcgccg gcgaaatcg ccggcggtt 
      cgaaggtca gggcgtggc acgtggtca aagagttgg 
      ggctttttg ggctctaca agggcgcga aggcctgtc 
      tgctgcggg atatcccct tcagtgcga tttacttcc 
      cgacttacg ctcacacga aggcgaaat tcgccgacg 
      agaccggtt ataaccacc ctttgtcct tgctagcgg 
      ctggggcta tcgccggtg tgccggctg ccgggctgg 
      tgaccccag ccgacgtca tcaagaccc ggctccagg 
      tggtggcaa gggccgggc agaccacct acaacgggg 
      tgtttgacg ccgccagga agatctacg tcgaggaag 
      ggtttaggg ccttttgga agggcgcca ttgcgagag 
      tgtgtcggt cgtccccgc agtttggtg tgacgctgg 
      tgacttacg aggttttgc aaagaatgt tatatattg 
      atttcggcg gaacgagac ccgctgggt ccgaattga 
      aggtcacgg cgttgggtg gggacgccc ccaagttgc 
      agaagccgg accatgtcg ggggttact cagtctcca 
      taccagtct tctcaggaa ttgaaacta agtttggtc 
      tttgcctgc caaaacttg gattgcttt cggaaagga 
      agtgatggc accaaatgc ataacaaag tttttttat 
      tttttaata ttttattcc taccttgtt cttacttca 
      gattttatt aaaacactc tttaagatt ctatttaat 
      aaagtatac acaataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY