Status
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Gene information
General information
TC016051 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1932468..1936813
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016051
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06572

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    remarks: eggs lost,
    Adult morphology
    remarks: eggs lost,
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: eggs lost,
    remarks - cuticles: later than schedule,
     
    8 days later, due to lost eggs in day 9
     

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016051 RA
      MGTRVFVGG LTYRVRERD IEKFFRKYG RIKEVAMKN 
      GFAFVEFDD YRDADDAVY ELNGKELLG ERVSVERAR 
      GTPRGCDQW RGSGGRGYG PPRGRSRDN REPDMRSHD 
      RYGPPTRTE YRLIVENLS SRVSWQDLK DYMRQAGEV 
      TYADAHKQR RNEGVVEFA SYSDMKNAI DKLDDTELN 
      GRRIRLVED RRRGSRRSR SSSSRSRSR SRSPRRRSR 
      SRSRSRSHR SSRSKSRDR SKSKSAAHS KSRSRSKSK 
      EASKSRSRS RSHSKRSKS RSCSKENGA KNRSRSRSM 
      SKEGSKSRS QSPTKENSP EKHD
    • >TC016051 RB
      MGTRVFVGG LTYRVRERD IEKFFRKYG RIKEVAMKN 
      GFAFVEFDD YRDADDAVY ELNGKELLG ERVSVERAR 
      GTPRGCDQW RGSGGRGYG PPRGRSRDK YGPPTRTEY 
      RLIVENLSS RVSWQDLKD YMRQAGEVT YADAHKQRR 
      NEGVVEFAS YSDMKNAID KLDDTELNG RRIRLVEDR 
      RRGSRRSRS SSSRSRSRS RSPRRRSRS RSRSRSHRS 
      SRSKSRDRS KSKSAAHSK SRSRSKSKE ASKSRSRSR 
      SHSKRSKSR SCSKENGAK NRSRSRSMS KEGSKSRSQ 
      SPTKENSPE KHD
    CDS sequence
    • >TC016051 RA
      atgggtacc cgcgttttt gttggcggt ctcacatac 
      agggtgaga gagcgggac atcgagaag ttttttaga 
      aagtatgga cgcattaag gaagttgcg atgaagaat 
      ggctttgcg tttgtggaa tttgacgac tatagagac 
      gcagatgat gctgtctat gagttgaat ggcaaggag 
      ctgcttgga gagcgagtt tctgtggaa agggctcgc 
      ggtactccg agaggatgt gaccagtgg cgcggtagc 
      ggtggccgt ggatacggc cccccgaga gggcgcagt 
      cgcgataac cgtgaacct gacatgcgg tcacatgat 
      aggtatggt ccaccgact aggacagag tatcgactg 
      attgtggag aatttgtca agccgagtg agctggcag 
      gaccttaaa gattacatg cggcaggct ggagaagtc 
      acgtatgca gatgctcat aagcaacga aggaacgaa 
      ggagtcgtt gagtttgct tcctattct gatatgaag 
      aatgccatt gataagttg gacgatact gagttgaat 
      ggtcgccga attcgtctg gttgaagac agacgtcgc 
      ggcagtaga agatcaagg tcgtctagt agtcggagc 
      cgttctcgc tccaggtct ccacggcgc cgttctcgt 
      tccaggtct agaagtcgt tcacatcgt tcaagccgt 
      tcaaaatct agggatcgt tcaaagagt aaatcagcc 
      gcacattca aaatcgcgt tctcgttct aagtcaaag 
      gaggcgtca aagtccaga tcgcgttcg aggtctcac 
      agtaagaga tctaagtct aggtcgtgt tcaaaagag 
      aacggggct aagaatagg tcccgttcg aggtcaatg 
      agtaaggaa ggaagtaaa tctaggagt cagtcgcca 
      accaaagaa aattcgcct gaaaaacat gattaa
    • >TC016051 RB
      atgggtacc cgcgttttt gttggcggt ctcacatac 
      agggtgaga gagcgggac atcgagaag ttttttaga 
      aagtatgga cgcattaag gaagttgcg atgaagaat 
      ggctttgcg tttgtggaa tttgacgac tatagagac 
      gcagatgat gctgtctat gagttgaat ggcaaggag 
      ctgcttgga gagcgagtt tctgtggaa agggctcgc 
      ggtactccg agaggatgt gaccagtgg cgcggtagc 
      ggtggccgt ggatacggc cccccgaga gggcgcagt 
      cgcgataag tatggtcca ccgactagg acagagtat 
      cgactgatt gtggagaat ttgtcaagc cgagtgagc 
      tggcaggac cttaaagat tacatgcgg caggctgga 
      gaagtcacg tatgcagat gctcataag caacgaagg 
      aacgaagga gtcgttgag tttgcttcc tattctgat 
      atgaagaat gccattgat aagttggac gatactgag 
      ttgaatggt cgccgaatt cgtctggtt gaagacaga 
      cgtcgcggc agtagaaga tcaaggtcg tctagtagt 
      cggagccgt tctcgctcc aggtctcca cggcgccgt 
      tctcgttcc aggtctaga agtcgttca catcgttca 
      agccgttca aaatctagg gatcgttca aagagtaaa 
      tcagccgca cattcaaaa tcgcgttct cgttctaag 
      tcaaaggag gcgtcaaag tccagatcg cgttcgagg 
      tctcacagt aagagatct aagtctagg tcgtgttca 
      aaagagaac ggggctaag aataggtcc cgttcgagg 
      tcaatgagt aaggaagga agtaaatct aggagtcag 
      tcgccaacc aaagaaaat tcgcctgaa aaacatgat 
      taa
    mRNA sequence
    • >TC016051 RA
      actttatgt ataactttg tttcggaag ttatgtttt 
      ttggaaatg aggcgtagt tgaagcgca gcaggcatt 
      ggccattgt cgagcaccg ccatcactt tagttgtgt 
      cgtgttggt agtttattt tcttcgtta ttgctacaa 
      attctcatc gaaaaaaga tgggtaccc gcgtttttg 
      ttggcggtc tcacataca gggtgagag agcgggaca 
      tcgagaagt tttttagaa agtatggac gcattaagg 
      aagttgcga tgaagaatg gctttgcgt ttgtggaat 
      ttgacgact atagagacg cagatgatg ctgtctatg 
      agttgaatg gcaaggagc tgcttggag agcgagttt 
      ctgtggaaa gggctcgcg gtactccga gaggatgtg 
      accagtggc gcggtagcg gtggccgtg gatacggcc 
      ccccgagag ggcgcagtc gcgataacc gtgaacctg 
      acatgcggt cacatgata ggtatggtc caccgacta 
      ggacagagt atcgactga ttgtggaga atttgtcaa 
      gccgagtga gctggcagg accttaaag attacatgc 
      ggcaggctg gagaagtca cgtatgcag atgctcata 
      agcaacgaa ggaacgaag gagtcgttg agtttgctt 
      cctattctg atatgaaga atgccattg ataagttgg 
      acgatactg agttgaatg gtcgccgaa ttcgtctgg 
      ttgaagaca gacgtcgcg gcagtagaa gatcaaggt 
      cgtctagta gtcggagcc gttctcgct ccaggtctc 
      cacggcgcc gttctcgtt ccaggtcta gaagtcgtt 
      cacatcgtt caagccgtt caaaatcta gggatcgtt 
      caaagagta aatcagccg cacattcaa aatcgcgtt 
      ctcgttcta agtcaaagg aggcgtcaa agtccagat 
      cgcgttcga ggtctcaca gtaagagat ctaagtcta 
      ggtcgtgtt caaaagaga acggggcta agaataggt 
      cccgttcga ggtcaatga gtaaggaag gaagtaaat 
      ctaggagtc agtcgccaa ccaaagaaa attcgcctg 
      aaaaacatg attaataca tagttgttt tttaagtaa 
      gtcatttgc ccgaattgt gacgcggca aaactgatc 
      gtaggacta tagctaact agttttaag atattattt 
      tgataataa ttgtctcag tctttgccg ttaagttga 
      tttagcatg gaccagagg ttttgctaa tagaaggct 
      ggttttggg tccagttag tcagcatat tctataatt 
      accgaaatc agctgatgt aaacatact gtttgcata 
      taaatgtct gatgtgttt tgtaatata aaaagaaat 
      gttcaaaat gtaacctaa ctgacttat aataacctt 
      atttttagt aggaacttg gctgtagaa atatgtctg 
      aaacaatct tacggtata catgttaca aaatctttt 
      tgaagtgtg acgttatta ggcctaagg gacacactc 
      ttggtaata catttgtga catatatat caatttaat 
      tgcatacct accgtgtag aatttttta catttattt 
      ttttataga atttctttg tggttttag tgatttact 
      ttaagtcaa attacgtta catcctaaa acactacat 
      cacatggat aacgtaggg taaacgtta gagactcgt 
      ccagtatat ttttttatg ttgtaactt tgtgatgta 
      gtgtttgtc taatgttga catttggat tgttaattt 
      ttcgtcttt atttttttt agttttgtt tccttgtta 
      agtgtcaac acatttata tatacatat ataactgta 
      atccgtaat ttaataaat agaatttaa cttc
    • >TC016051 RB
      actttatgt ataactttg tttcggaag ttatgtttt 
      ttggaaatg aggcgtagt tgaagcgca gcaggcatt 
      ggccattgt cgagcaccg ccatcactt tagttgtgt 
      cgtgttggt agtttattt tcttcgtta ttgctacaa 
      attctcatc gaaaaaaga tgggtaccc gcgtttttg 
      ttggcggtc tcacataca gggtgagag agcgggaca 
      tcgagaagt tttttagaa agtatggac gcattaagg 
      aagttgcga tgaagaatg gctttgcgt ttgtggaat 
      ttgacgact atagagacg cagatgatg ctgtctatg 
      agttgaatg gcaaggagc tgcttggag agcgagttt 
      ctgtggaaa gggctcgcg gtactccga gaggatgtg 
      accagtggc gcggtagcg gtggccgtg gatacggcc 
      ccccgagag ggcgcagtc gcgataagt atggtccac 
      cgactagga cagagtatc gactgattg tggagaatt 
      tgtcaagcc gagtgagct ggcaggacc ttaaagatt 
      acatgcggc aggctggag aagtcacgt atgcagatg 
      ctcataagc aacgaagga acgaaggag tcgttgagt 
      ttgcttcct attctgata tgaagaatg ccattgata 
      agttggacg atactgagt tgaatggtc gccgaattc 
      gtctggttg aagacagac gtcgcggca gtagaagat 
      caaggtcgt ctagtagtc ggagccgtt ctcgctcca 
      ggtctccac ggcgccgtt ctcgttcca ggtctagaa 
      gtcgttcac atcgttcaa gccgttcaa aatctaggg 
      atcgttcaa agagtaaat cagccgcac attcaaaat 
      cgcgttctc gttctaagt caaaggagg cgtcaaagt 
      ccagatcgc gttcgaggt ctcacagta agagatcta 
      agtctaggt cgtgttcaa aagagaacg gggctaaga 
      ataggtccc gttcgaggt caatgagta aggaaggaa 
      gtaaatcta ggagtcagt cgccaacca aagaaaatt 
      cgcctgaaa aacatgatt aatacatag ttgtttttt 
      aagtaagtc atttgcccg aattgtgac gcggcaaaa 
      ctgatcgta ggactatag ctaactagt tttaagata 
      ttattttga taataattg tctcagtct ttgccgtta 
      agttgattt agcatggac cagaggttt tgctaatag 
      aaggctggt tttgggtcc agttagtca gcatattct 
      ataattacc gaaatcagc tgatgtaaa catactgtt 
      tgcatataa atgtctgat gtgttttgt aatataaaa 
      agaaatgtt caaaatgta acctaactg acttataat 
      aaccttatt tttagtagg aacttggct gtagaaata 
      tgtctgaaa caatcttac ggtatacat gttacaaaa 
      tctttttga agtgtgacg ttattaggc ctaagggac 
      acactcttg gtaatacat ttgtgacat atatatcaa 
      tttaattgc atacctacc gtgtagaat tttttacat 
      ttatttttt tatagaatt tctttgtgg ttttagtga 
      tttacttta agtcaaatt acgttacat cctaaaaca 
      ctacatcac atggataac gtagggtaa acgttagag 
      actcgtcca gtatatttt tttatgttg taactttgt 
      gatgtagtg tttgtctaa tgttgacat ttggattgt 
      taatttttc gtctttatt ttttttagt tttgtttcc 
      ttgttaagt gtcaacaca tttatatat acatatata 
      actgtaatc cgtaattta ataaataga atttaactt 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY