Status
Please wait ...
Gene information
General information
TC016051 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1932468..1936813
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC016051
    iBeetle screen iB_06572

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection

    remarks: eggs lost,
    Adult morphology
    remarks: eggs lost,
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: eggs lost,
    remarks - cuticles: later than schedule,
     
    8 days later, due to lost eggs in day 9
     

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016051 RA
      MGTRVFVGG LTYRVRERD IEKFFRKYG RIKEVAMKN 
GFAFVEFDD YRDADDAVY ELNGKELLG ERVSVERAR 
GTPRGCDQW RGSGGRGYG PPRGRSRDN REPDMRSHD 
RYGPPTRTE YRLIVENLS SRVSWQDLK DYMRQAGEV 
TYADAHKQR RNEGVVEFA SYSDMKNAI DKLDDTELN 
GRRIRLVED RRRGSRRSR SSSSRSRSR SRSPRRRSR 
SRSRSRSHR SSRSKSRDR SKSKSAAHS KSRSRSKSK 
EASKSRSRS RSHSKRSKS RSCSKENGA KNRSRSRSM 
SKEGSKSRS QSPTKENSP EKHD
    • >TC016051 RB
      MGTRVFVGG LTYRVRERD IEKFFRKYG RIKEVAMKN 
GFAFVEFDD YRDADDAVY ELNGKELLG ERVSVERAR 
GTPRGCDQW RGSGGRGYG PPRGRSRDK YGPPTRTEY 
RLIVENLSS RVSWQDLKD YMRQAGEVT YADAHKQRR 
NEGVVEFAS YSDMKNAID KLDDTELNG RRIRLVEDR 
RRGSRRSRS SSSRSRSRS RSPRRRSRS RSRSRSHRS 
SRSKSRDRS KSKSAAHSK SRSRSKSKE ASKSRSRSR 
SHSKRSKSR SCSKENGAK NRSRSRSMS KEGSKSRSQ 
SPTKENSPE KHD
    CDS sequence
    • >TC016051 RA
      atgggtacc cgcgttttt gttggcggt ctcacatac 
agggtgaga gagcgggac atcgagaag ttttttaga 
aagtatgga cgcattaag gaagttgcg atgaagaat 
ggctttgcg tttgtggaa tttgacgac tatagagac 
gcagatgat gctgtctat gagttgaat ggcaaggag 
ctgcttgga gagcgagtt tctgtggaa agggctcgc 
ggtactccg agaggatgt gaccagtgg cgcggtagc 
ggtggccgt ggatacggc cccccgaga gggcgcagt 
cgcgataac cgtgaacct gacatgcgg tcacatgat 
aggtatggt ccaccgact aggacagag tatcgactg 
attgtggag aatttgtca agccgagtg agctggcag 
gaccttaaa gattacatg cggcaggct ggagaagtc 
acgtatgca gatgctcat aagcaacga aggaacgaa 
ggagtcgtt gagtttgct tcctattct gatatgaag 
aatgccatt gataagttg gacgatact gagttgaat 
ggtcgccga attcgtctg gttgaagac agacgtcgc 
ggcagtaga agatcaagg tcgtctagt agtcggagc 
cgttctcgc tccaggtct ccacggcgc cgttctcgt 
tccaggtct agaagtcgt tcacatcgt tcaagccgt 
tcaaaatct agggatcgt tcaaagagt aaatcagcc 
gcacattca aaatcgcgt tctcgttct aagtcaaag 
gaggcgtca aagtccaga tcgcgttcg aggtctcac 
agtaagaga tctaagtct aggtcgtgt tcaaaagag 
aacggggct aagaatagg tcccgttcg aggtcaatg 
agtaaggaa ggaagtaaa tctaggagt cagtcgcca 
accaaagaa aattcgcct gaaaaacat gattaa
    • >TC016051 RB
      atgggtacc cgcgttttt gttggcggt ctcacatac 
agggtgaga gagcgggac atcgagaag ttttttaga 
aagtatgga cgcattaag gaagttgcg atgaagaat 
ggctttgcg tttgtggaa tttgacgac tatagagac 
gcagatgat gctgtctat gagttgaat ggcaaggag 
ctgcttgga gagcgagtt tctgtggaa agggctcgc 
ggtactccg agaggatgt gaccagtgg cgcggtagc 
ggtggccgt ggatacggc cccccgaga gggcgcagt 
cgcgataag tatggtcca ccgactagg acagagtat 
cgactgatt gtggagaat ttgtcaagc cgagtgagc 
tggcaggac cttaaagat tacatgcgg caggctgga 
gaagtcacg tatgcagat gctcataag caacgaagg 
aacgaagga gtcgttgag tttgcttcc tattctgat 
atgaagaat gccattgat aagttggac gatactgag 
ttgaatggt cgccgaatt cgtctggtt gaagacaga 
cgtcgcggc agtagaaga tcaaggtcg tctagtagt 
cggagccgt tctcgctcc aggtctcca cggcgccgt 
tctcgttcc aggtctaga agtcgttca catcgttca 
agccgttca aaatctagg gatcgttca aagagtaaa 
tcagccgca cattcaaaa tcgcgttct cgttctaag 
tcaaaggag gcgtcaaag tccagatcg cgttcgagg 
tctcacagt aagagatct aagtctagg tcgtgttca 
aaagagaac ggggctaag aataggtcc cgttcgagg 
tcaatgagt aaggaagga agtaaatct aggagtcag 
tcgccaacc aaagaaaat tcgcctgaa aaacatgat 
taa
    mRNA sequence
    • >TC016051 RA
      actttatgt ataactttg tttcggaag ttatgtttt 
ttggaaatg aggcgtagt tgaagcgca gcaggcatt 
ggccattgt cgagcaccg ccatcactt tagttgtgt 
cgtgttggt agtttattt tcttcgtta ttgctacaa 
attctcatc gaaaaaaga tgggtaccc gcgtttttg 
ttggcggtc tcacataca gggtgagag agcgggaca 
tcgagaagt tttttagaa agtatggac gcattaagg 
aagttgcga tgaagaatg gctttgcgt ttgtggaat 
ttgacgact atagagacg cagatgatg ctgtctatg 
agttgaatg gcaaggagc tgcttggag agcgagttt 
ctgtggaaa gggctcgcg gtactccga gaggatgtg 
accagtggc gcggtagcg gtggccgtg gatacggcc 
ccccgagag ggcgcagtc gcgataacc gtgaacctg 
acatgcggt cacatgata ggtatggtc caccgacta 
ggacagagt atcgactga ttgtggaga atttgtcaa 
gccgagtga gctggcagg accttaaag attacatgc 
ggcaggctg gagaagtca cgtatgcag atgctcata 
agcaacgaa ggaacgaag gagtcgttg agtttgctt 
cctattctg atatgaaga atgccattg ataagttgg 
acgatactg agttgaatg gtcgccgaa ttcgtctgg 
ttgaagaca gacgtcgcg gcagtagaa gatcaaggt 
cgtctagta gtcggagcc gttctcgct ccaggtctc 
cacggcgcc gttctcgtt ccaggtcta gaagtcgtt 
cacatcgtt caagccgtt caaaatcta gggatcgtt 
caaagagta aatcagccg cacattcaa aatcgcgtt 
ctcgttcta agtcaaagg aggcgtcaa agtccagat 
cgcgttcga ggtctcaca gtaagagat ctaagtcta 
ggtcgtgtt caaaagaga acggggcta agaataggt 
cccgttcga ggtcaatga gtaaggaag gaagtaaat 
ctaggagtc agtcgccaa ccaaagaaa attcgcctg 
aaaaacatg attaataca tagttgttt tttaagtaa 
gtcatttgc ccgaattgt gacgcggca aaactgatc 
gtaggacta tagctaact agttttaag atattattt 
tgataataa ttgtctcag tctttgccg ttaagttga 
tttagcatg gaccagagg ttttgctaa tagaaggct 
ggttttggg tccagttag tcagcatat tctataatt 
accgaaatc agctgatgt aaacatact gtttgcata 
taaatgtct gatgtgttt tgtaatata aaaagaaat 
gttcaaaat gtaacctaa ctgacttat aataacctt 
atttttagt aggaacttg gctgtagaa atatgtctg 
aaacaatct tacggtata catgttaca aaatctttt 
tgaagtgtg acgttatta ggcctaagg gacacactc 
ttggtaata catttgtga catatatat caatttaat 
tgcatacct accgtgtag aatttttta catttattt 
ttttataga atttctttg tggttttag tgatttact 
ttaagtcaa attacgtta catcctaaa acactacat 
cacatggat aacgtaggg taaacgtta gagactcgt 
ccagtatat ttttttatg ttgtaactt tgtgatgta 
gtgtttgtc taatgttga catttggat tgttaattt 
ttcgtcttt atttttttt agttttgtt tccttgtta 
agtgtcaac acatttata tatacatat ataactgta 
atccgtaat ttaataaat agaatttaa cttc
    • >TC016051 RB
      actttatgt ataactttg tttcggaag ttatgtttt 
ttggaaatg aggcgtagt tgaagcgca gcaggcatt 
ggccattgt cgagcaccg ccatcactt tagttgtgt 
cgtgttggt agtttattt tcttcgtta ttgctacaa 
attctcatc gaaaaaaga tgggtaccc gcgtttttg 
ttggcggtc tcacataca gggtgagag agcgggaca 
tcgagaagt tttttagaa agtatggac gcattaagg 
aagttgcga tgaagaatg gctttgcgt ttgtggaat 
ttgacgact atagagacg cagatgatg ctgtctatg 
agttgaatg gcaaggagc tgcttggag agcgagttt 
ctgtggaaa gggctcgcg gtactccga gaggatgtg 
accagtggc gcggtagcg gtggccgtg gatacggcc 
ccccgagag ggcgcagtc gcgataagt atggtccac 
cgactagga cagagtatc gactgattg tggagaatt 
tgtcaagcc gagtgagct ggcaggacc ttaaagatt 
acatgcggc aggctggag aagtcacgt atgcagatg 
ctcataagc aacgaagga acgaaggag tcgttgagt 
ttgcttcct attctgata tgaagaatg ccattgata 
agttggacg atactgagt tgaatggtc gccgaattc 
gtctggttg aagacagac gtcgcggca gtagaagat 
caaggtcgt ctagtagtc ggagccgtt ctcgctcca 
ggtctccac ggcgccgtt ctcgttcca ggtctagaa 
gtcgttcac atcgttcaa gccgttcaa aatctaggg 
atcgttcaa agagtaaat cagccgcac attcaaaat 
cgcgttctc gttctaagt caaaggagg cgtcaaagt 
ccagatcgc gttcgaggt ctcacagta agagatcta 
agtctaggt cgtgttcaa aagagaacg gggctaaga 
ataggtccc gttcgaggt caatgagta aggaaggaa 
gtaaatcta ggagtcagt cgccaacca aagaaaatt 
cgcctgaaa aacatgatt aatacatag ttgtttttt 
aagtaagtc atttgcccg aattgtgac gcggcaaaa 
ctgatcgta ggactatag ctaactagt tttaagata 
ttattttga taataattg tctcagtct ttgccgtta 
agttgattt agcatggac cagaggttt tgctaatag 
aaggctggt tttgggtcc agttagtca gcatattct 
ataattacc gaaatcagc tgatgtaaa catactgtt 
tgcatataa atgtctgat gtgttttgt aatataaaa 
agaaatgtt caaaatgta acctaactg acttataat 
aaccttatt tttagtagg aacttggct gtagaaata 
tgtctgaaa caatcttac ggtatacat gttacaaaa 
tctttttga agtgtgacg ttattaggc ctaagggac 
acactcttg gtaatacat ttgtgacat atatatcaa 
tttaattgc atacctacc gtgtagaat tttttacat 
ttatttttt tatagaatt tctttgtgg ttttagtga 
tttacttta agtcaaatt acgttacat cctaaaaca 
ctacatcac atggataac gtagggtaa acgttagag 
actcgtcca gtatatttt tttatgttg taactttgt 
gatgtagtg tttgtctaa tgttgacat ttggattgt 
taatttttc gtctttatt ttttttagt tttgtttcc 
ttgttaagt gtcaacaca tttatatat acatatata 
actgtaatc cgtaattta ataaataga atttaactt 
c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY