| General information | |||
| TC016051 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 1932468..1936813 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC016051 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06572 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
remarks: eggs lost,
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: eggs lost,
remarks - cuticles: later than schedule,
|
8 days later, due to lost eggs in day 9
|
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC016051 RA
MGTRVFVGG LTYRVRERD IEKFFRKYG RIKEVAMKN GFAFVEFDD YRDADDAVY ELNGKELLG ERVSVERAR GTPRGCDQW RGSGGRGYG PPRGRSRDN REPDMRSHD RYGPPTRTE YRLIVENLS SRVSWQDLK DYMRQAGEV TYADAHKQR RNEGVVEFA SYSDMKNAI DKLDDTELN GRRIRLVED RRRGSRRSR SSSSRSRSR SRSPRRRSR SRSRSRSHR SSRSKSRDR SKSKSAAHS KSRSRSKSK EASKSRSRS RSHSKRSKS RSCSKENGA KNRSRSRSM SKEGSKSRS QSPTKENSP EKHD
- >TC016051 RB
MGTRVFVGG LTYRVRERD IEKFFRKYG RIKEVAMKN GFAFVEFDD YRDADDAVY ELNGKELLG ERVSVERAR GTPRGCDQW RGSGGRGYG PPRGRSRDK YGPPTRTEY RLIVENLSS RVSWQDLKD YMRQAGEVT YADAHKQRR NEGVVEFAS YSDMKNAID KLDDTELNG RRIRLVEDR RRGSRRSRS SSSRSRSRS RSPRRRSRS RSRSRSHRS SRSKSRDRS KSKSAAHSK SRSRSKSKE ASKSRSRSR SHSKRSKSR SCSKENGAK NRSRSRSMS KEGSKSRSQ SPTKENSPE KHD
- >TC016051 RA
atgggtacc cgcgttttt gttggcggt ctcacatac agggtgaga gagcgggac atcgagaag ttttttaga aagtatgga cgcattaag gaagttgcg atgaagaat ggctttgcg tttgtggaa tttgacgac tatagagac gcagatgat gctgtctat gagttgaat ggcaaggag ctgcttgga gagcgagtt tctgtggaa agggctcgc ggtactccg agaggatgt gaccagtgg cgcggtagc ggtggccgt ggatacggc cccccgaga gggcgcagt cgcgataac cgtgaacct gacatgcgg tcacatgat aggtatggt ccaccgact aggacagag tatcgactg attgtggag aatttgtca agccgagtg agctggcag gaccttaaa gattacatg cggcaggct ggagaagtc acgtatgca gatgctcat aagcaacga aggaacgaa ggagtcgtt gagtttgct tcctattct gatatgaag aatgccatt gataagttg gacgatact gagttgaat ggtcgccga attcgtctg gttgaagac agacgtcgc ggcagtaga agatcaagg tcgtctagt agtcggagc cgttctcgc tccaggtct ccacggcgc cgttctcgt tccaggtct agaagtcgt tcacatcgt tcaagccgt tcaaaatct agggatcgt tcaaagagt aaatcagcc gcacattca aaatcgcgt tctcgttct aagtcaaag gaggcgtca aagtccaga tcgcgttcg aggtctcac agtaagaga tctaagtct aggtcgtgt tcaaaagag aacggggct aagaatagg tcccgttcg aggtcaatg agtaaggaa ggaagtaaa tctaggagt cagtcgcca accaaagaa aattcgcct gaaaaacat gattaa
- >TC016051 RB
atgggtacc cgcgttttt gttggcggt ctcacatac agggtgaga gagcgggac atcgagaag ttttttaga aagtatgga cgcattaag gaagttgcg atgaagaat ggctttgcg tttgtggaa tttgacgac tatagagac gcagatgat gctgtctat gagttgaat ggcaaggag ctgcttgga gagcgagtt tctgtggaa agggctcgc ggtactccg agaggatgt gaccagtgg cgcggtagc ggtggccgt ggatacggc cccccgaga gggcgcagt cgcgataag tatggtcca ccgactagg acagagtat cgactgatt gtggagaat ttgtcaagc cgagtgagc tggcaggac cttaaagat tacatgcgg caggctgga gaagtcacg tatgcagat gctcataag caacgaagg aacgaagga gtcgttgag tttgcttcc tattctgat atgaagaat gccattgat aagttggac gatactgag ttgaatggt cgccgaatt cgtctggtt gaagacaga cgtcgcggc agtagaaga tcaaggtcg tctagtagt cggagccgt tctcgctcc aggtctcca cggcgccgt tctcgttcc aggtctaga agtcgttca catcgttca agccgttca aaatctagg gatcgttca aagagtaaa tcagccgca cattcaaaa tcgcgttct cgttctaag tcaaaggag gcgtcaaag tccagatcg cgttcgagg tctcacagt aagagatct aagtctagg tcgtgttca aaagagaac ggggctaag aataggtcc cgttcgagg tcaatgagt aaggaagga agtaaatct aggagtcag tcgccaacc aaagaaaat tcgcctgaa aaacatgat taa
- >TC016051 RA
actttatgt ataactttg tttcggaag ttatgtttt ttggaaatg aggcgtagt tgaagcgca gcaggcatt ggccattgt cgagcaccg ccatcactt tagttgtgt cgtgttggt agtttattt tcttcgtta ttgctacaa attctcatc gaaaaaaga tgggtaccc gcgtttttg ttggcggtc tcacataca gggtgagag agcgggaca tcgagaagt tttttagaa agtatggac gcattaagg aagttgcga tgaagaatg gctttgcgt ttgtggaat ttgacgact atagagacg cagatgatg ctgtctatg agttgaatg gcaaggagc tgcttggag agcgagttt ctgtggaaa gggctcgcg gtactccga gaggatgtg accagtggc gcggtagcg gtggccgtg gatacggcc ccccgagag ggcgcagtc gcgataacc gtgaacctg acatgcggt cacatgata ggtatggtc caccgacta ggacagagt atcgactga ttgtggaga atttgtcaa gccgagtga gctggcagg accttaaag attacatgc ggcaggctg gagaagtca cgtatgcag atgctcata agcaacgaa ggaacgaag gagtcgttg agtttgctt cctattctg atatgaaga atgccattg ataagttgg acgatactg agttgaatg gtcgccgaa ttcgtctgg ttgaagaca gacgtcgcg gcagtagaa gatcaaggt cgtctagta gtcggagcc gttctcgct ccaggtctc cacggcgcc gttctcgtt ccaggtcta gaagtcgtt cacatcgtt caagccgtt caaaatcta gggatcgtt caaagagta aatcagccg cacattcaa aatcgcgtt ctcgttcta agtcaaagg aggcgtcaa agtccagat cgcgttcga ggtctcaca gtaagagat ctaagtcta ggtcgtgtt caaaagaga acggggcta agaataggt cccgttcga ggtcaatga gtaaggaag gaagtaaat ctaggagtc agtcgccaa ccaaagaaa attcgcctg aaaaacatg attaataca tagttgttt tttaagtaa gtcatttgc ccgaattgt gacgcggca aaactgatc gtaggacta tagctaact agttttaag atattattt tgataataa ttgtctcag tctttgccg ttaagttga tttagcatg gaccagagg ttttgctaa tagaaggct ggttttggg tccagttag tcagcatat tctataatt accgaaatc agctgatgt aaacatact gtttgcata taaatgtct gatgtgttt tgtaatata aaaagaaat gttcaaaat gtaacctaa ctgacttat aataacctt atttttagt aggaacttg gctgtagaa atatgtctg aaacaatct tacggtata catgttaca aaatctttt tgaagtgtg acgttatta ggcctaagg gacacactc ttggtaata catttgtga catatatat caatttaat tgcatacct accgtgtag aatttttta catttattt ttttataga atttctttg tggttttag tgatttact ttaagtcaa attacgtta catcctaaa acactacat cacatggat aacgtaggg taaacgtta gagactcgt ccagtatat ttttttatg ttgtaactt tgtgatgta gtgtttgtc taatgttga catttggat tgttaattt ttcgtcttt atttttttt agttttgtt tccttgtta agtgtcaac acatttata tatacatat ataactgta atccgtaat ttaataaat agaatttaa cttc
- >TC016051 RB
actttatgt ataactttg tttcggaag ttatgtttt ttggaaatg aggcgtagt tgaagcgca gcaggcatt ggccattgt cgagcaccg ccatcactt tagttgtgt cgtgttggt agtttattt tcttcgtta ttgctacaa attctcatc gaaaaaaga tgggtaccc gcgtttttg ttggcggtc tcacataca gggtgagag agcgggaca tcgagaagt tttttagaa agtatggac gcattaagg aagttgcga tgaagaatg gctttgcgt ttgtggaat ttgacgact atagagacg cagatgatg ctgtctatg agttgaatg gcaaggagc tgcttggag agcgagttt ctgtggaaa gggctcgcg gtactccga gaggatgtg accagtggc gcggtagcg gtggccgtg gatacggcc ccccgagag ggcgcagtc gcgataagt atggtccac cgactagga cagagtatc gactgattg tggagaatt tgtcaagcc gagtgagct ggcaggacc ttaaagatt acatgcggc aggctggag aagtcacgt atgcagatg ctcataagc aacgaagga acgaaggag tcgttgagt ttgcttcct attctgata tgaagaatg ccattgata agttggacg atactgagt tgaatggtc gccgaattc gtctggttg aagacagac gtcgcggca gtagaagat caaggtcgt ctagtagtc ggagccgtt ctcgctcca ggtctccac ggcgccgtt ctcgttcca ggtctagaa gtcgttcac atcgttcaa gccgttcaa aatctaggg atcgttcaa agagtaaat cagccgcac attcaaaat cgcgttctc gttctaagt caaaggagg cgtcaaagt ccagatcgc gttcgaggt ctcacagta agagatcta agtctaggt cgtgttcaa aagagaacg gggctaaga ataggtccc gttcgaggt caatgagta aggaaggaa gtaaatcta ggagtcagt cgccaacca aagaaaatt cgcctgaaa aacatgatt aatacatag ttgtttttt aagtaagtc atttgcccg aattgtgac gcggcaaaa ctgatcgta ggactatag ctaactagt tttaagata ttattttga taataattg tctcagtct ttgccgtta agttgattt agcatggac cagaggttt tgctaatag aaggctggt tttgggtcc agttagtca gcatattct ataattacc gaaatcagc tgatgtaaa catactgtt tgcatataa atgtctgat gtgttttgt aatataaaa agaaatgtt caaaatgta acctaactg acttataat aaccttatt tttagtagg aacttggct gtagaaata tgtctgaaa caatcttac ggtatacat gttacaaaa tctttttga agtgtgacg ttattaggc ctaagggac acactcttg gtaatacat ttgtgacat atatatcaa tttaattgc atacctacc gtgtagaat tttttacat ttatttttt tatagaatt tctttgtgg ttttagtga tttacttta agtcaaatt acgttacat cctaaaaca ctacatcac atggataac gtagggtaa acgttagag actcgtcca gtatatttt tttatgttg taactttgt gatgtagtg tttgtctaa tgttgacat ttggattgt taatttttc gtctttatt ttttttagt tttgtttcc ttgttaagt gtcaacaca tttatatat acatatata actgtaatc cgtaattta ataaataga atttaactt c
