Status
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Gene information
General information
TC004115 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1831387..1834584
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004115
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06571

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004115 RA
      MESASGLAV LEDEVLHNE TKALWVFAY GSLCWKPGF 
QFNKAVTGY VQGFHRRFW QGNTTHRGT EEKPGRVAT 
LVENSKGLV HGVAFAVSG EAAIPYLSK RECELGGYS 
SVFTTFYPV SGEPFKVLL YVATPKNPL WLGDAQIAD 
IADQIVDCR GPSGYNVEY VLRLANFMK HHFPEHDDQ 
HLFHLEEAV LRRVKERNM CLKTLMGDG EGCVTFVKV 
ERRRSTDSS DDNPDRIET FQHTTRVPE KTLRCLNI
    CDS sequence
    • >TC004115 RA
      atggaatca gcaagtggc ttagcggtc ctcgaagat 
gaggttctg cacaacgaa accaaagct ttatgggtc 
tttgcttac ggctccctg tgctggaaa cccggcttc 
cagtttaac aaagctgtc accggctac gtccaaggg 
tttcaccgc cggttctgg caggggaac accacgcac 
cgggggaca gaagaaaag cccggtcgt gtcgccact 
ttggtcgaa aacagtaag ggcttggtc cacggggtc 
gctttcgcg gtgagcgga gaggccgcg attccgtac 
ttgtcgaag agggagtgc gagttggga gggtacagt 
tcggtgttc accaccttc taccccgtc agcggggag 
ccgttcaag gtgctgctg tacgtggcc acgccgaag 
aacccgctc tggctggga gatgcccaa attgcggac 
atcgccgac cagattgtg gactgccgg gggcccagc 
gggtacaac gtcgagtac gtgctgcga ctggccaat 
ttcatgaag caccacttc ccggagcac gacgaccag 
cacctgttt cacctggag gaggcggtc ttgaggagg 
gtcaaggag cgcaacatg tgcctcaaa accctcatg 
ggggacggc gaaggctgc gtcaccttc gtcaaagtc 
gagcggcgg aggtccacg gacagctcg gacgacaat 
ccggaccgg atagagacg ttccaacac acgacgcgg 
gttccagaa aaaacgcta cgttgcttg aatatttag
    mRNA sequence
    • >TC004115 RA
      acacgtccc tgtataccg cggtgtccg tctgcgtcc 
gaagtgtag caaaacgca tgtgtgatc tgcatattt 
aaaaataaa ttcgcgcgc ttgaaattt gtcggagaa 
aagctcgtg cgtgtagtt agtgtccag ttcgagttg 
ggattgatc gaatctttc tgacagctg tctcggaag 
tgttgcatc agctatgga atcagcaag tggcttagc 
ggtcctcga agatgaggt tctgcacaa cgaaaccaa 
agctttatg ggtctttgc ttacggctc cctgtgctg 
gaaacccgg cttccagtt taacaaagc tgtcaccgg 
ctacgtcca agggtttca ccgccggtt ctggcaggg 
gaacaccac gcaccgggg gacagaaga aaagcccgg 
tcgtgtcgc cactttggt cgaaaacag taagggctt 
ggtccacgg ggtcgcttt cgcggtgag cggagaggc 
cgcgattcc gtacttgtc gaagaggga gtgcgagtt 
gggagggta cagttcggt gttcaccac cttctaccc 
cgtcagcgg ggagccgtt caaggtgct gctgtacgt 
ggccacgcc gaagaaccc gctctggct gggagatgc 
ccaaattgc ggacatcgc cgaccagat tgtggactg 
ccgggggcc cagcgggta caacgtcga gtacgtgct 
gcgactggc caatttcat gaagcacca cttcccgga 
gcacgacga ccagcacct gtttcacct ggaggaggc 
ggtcttgag gagggtcaa ggagcgcaa catgtgcct 
caaaaccct catggggga cggcgaagg ctgcgtcac 
cttcgtcaa agtcgagcg gcggaggtc cacggacag 
ctcggacga caatccgga ccggataga gacgttcca 
acacacgac gcgggttcc agaaaaaac gctacgttg 
cttgaatat ttagcgatt gtttattta tttattata 
gcaacggtg ctaccgtgt agtaccaat tcgttcagt 
gttgattgt gattttatt tttgcattt aagtatatt 
tttatatga gtcgtgcgg tccagtcaa cagttacag 
gttcagaga tatatttat attgttttg tattggccc 
aactgtgat tttataatt aacaaaata aactcgtat 
ttaataacg cgatttaaa tttaattac ccgattgac 
ctcacctca gaaggacat tccgtagcc tttgaccat 
gaattgtta cggagtgtt tctcctatt aactgtgac 
ggttcgtca acctttgat aaacactgg aacaaggtt 
ccttatcct tgtttctcg gagtgaaat gataagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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