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Gene information
General information
TC004122 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1716953..1718987
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004122
    iBeetle screen iB_06568

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva dorsal irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: In strong cases head or thoracic segments are completely not present.
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: In strong cases head or thoracic segments are completely not present.
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: In strong cases head or thoracic segments are completely not present.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004122 RA
      MSQNEHKFV YSHGDKSVE VDIKIPFEG SVGELVHVL 
      ILKNNLPIY VEKDLAENL SQFVNDWSI KHNNEHTAS 
      LIDDARNDK TNIDEITKE WEKLMREEM AEYGERRCA 
      SDEELFATA YHKMVHSPA LETMLQLEH MYSKTVRAK 
      TEEKNRCIQ NLSMKQTEE MNHAVDRLE QDMTESKIN 
      ELVARHYEA HSLLKVKLT SELDTIKEA QRREYREWL 
      MQMLEQNQA TSSLPTPNS PLTPHTPQP AIYSSECAR 
      SIDTPILEE SFTIHLGSQ LKQMHNIRI LSANVMELC 
      AVEDNDQSS EPKPQLLQT ALALYSSDL SGLVLMTDN 
      KIGSRLTQD FQEICQRTT EFHFPHIDD QLDKISNTA 
      QLYLKSQKE NSNRNINSE LLQAGDFYI TRHSNLAQV 
      HVIFHMVSD DSLRGSDIN SRHPVVLGL RNILKTACS 
      NDITSLTIP LLLQYEMTE EMTISWCEK RAELVFKCV 
      KGFMIEMAS WGGSELKNL QFMLPQGIS SRVFQSLTE 
      MLPRIFKVS NPKILK
    CDS sequence
    • >TC004122 RA
      atgtcccag aacgaacat aaatttgtt tactcacac 
      ggcgacaaa tcagttgaa gtcgacatt aaaatcccg 
      tttgagggg tccgttggg gagttagtc catgttttg 
      atccttaaa aacaactta ccaatatat gtggagaaa 
      gatcttgct gagaacttg tcccaattt gtgaacgac 
      tggagcata aagcacaac aatgaacac acggcgtct 
      ctaatagac gacgcaaga aacgacaaa actaacatt 
      gacgagata acgaaagaa tgggagaaa ctaatgcgg 
      gaggaaatg gccgaatat ggggagcgt agatgcgcc 
      tcagatgag gagcttttc gccacagct taccacaaa 
      atggtgcat tcaccagcg ctagagaca atgctacaa 
      ctcgagcac atgtactcg aagaccgtg cgtgctaaa 
      accgaggaa aaaaaccgt tgtatccag aacctatca 
      atgaaacaa actgaggag atgaaccat gccgtggac 
      aggctggaa caggacatg accgagtca aaaatcaac 
      gaattagtg gcgcgacac tacgaggcc cattcgcta 
      ctcaaagtc aaactaacc agcgagtta gacacaatc 
      aaggaggcc caaaggagg gagtaccgg gaatggctg 
      atgcaaatg ctggagcaa aaccaagcg acgagttca 
      ctaccaact cccaactcg cctttaacg ccacatacc 
      ccccaaccc gcgatttat tcgtcagag tgcgcccgg 
      agcatagac acccccatt ttggaggag agtttcacc 
      attcatttg gggtctcag ctgaaacag atgcacaat 
      atccgcatt ttgtcggca aatgtcatg gagttgtgt 
      gccgtggaa gacaacgat cagtcgtct gagccaaaa 
      ccacaactt ctacaaacc gccctggct ttgtactca 
      agcgatctc tcggggctt gttctcatg actgacaat 
      aaaattggg tcacgactc acccaagac tttcaagaa 
      atttgccag agaactact gaattccac ttcccgcat 
      attgacgac cagttggac aaaatctcg aacactgct 
      cagctgtac ctaaagtcg cagaaggag aacagcaac 
      agaaacata aacagcgag ttgttacaa gccggcgat 
      ttttacatc accagacat tccaactta gcacaagtc 
      catgttatt ttccatatg gtttcggac gatagctta 
      agaggcagt gatattaac agcagacat cccgtcgtg 
      ttagggttg aggaacatc ctaaagacg gcctgctcc 
      aacgatatc acttcactg accatcccg ttgttgcta 
      caatacgag atgacagag gaaatgacc atatcatgg 
      tgcgaaaaa cgggcagaa ctggttttt aagtgcgtc 
      aaaggtttc atgattgaa atggcctca tggggcggc 
      tccgagctg aaaaaccta cagtttatg ctgccgcaa 
      ggcatctcg agcagagtt ttccagtcg ctaaccgaa 
      atgttacca agaatattt aaagtatca aatcccaaa 
      atattgaag taa
    mRNA sequence
    • >TC004122 RA
      tgtccaagc gtttgtcga aaataataa taattatta 
      ttattatgg ccaaccgtt ttttttgtt gacgattca 
      cactgacag cgaacagct gtcaaaata aaggatgaa 
      caagttaga ggttaactc caacataaa atttaattt 
      attagggaa cgtaataat cattgatgt tagcgcttt 
      atgttgaat gtagtgtag tgtctagtc aaacatgtc 
      ccagaacga acataaatt tgtttactc acacggcga 
      caaatcagt tgaagtcga cattaaaat cccgtttga 
      ggggtccgt tggggagtt agtccatgt tttgatcct 
      taaaaacaa cttaccaat atatgtgga gaaagatct 
      tgctgagaa cttgtccca atttgtgaa cgactggag 
      cataaagca caacaatga acacacggc gtctctaat 
      agacgacgc aagaaacga caaaactaa cattgacga 
      gataacgaa agaatggga gaaactaat gcgggagga 
      aatggccga atatgggga gcgtagatg cgcctcaga 
      tgaggagct tttcgccac agcttacca caaaatggt 
      gcattcacc agcgctaga gacaatgct acaactcga 
      gcacatgta ctcgaagac cgtgcgtgc taaaaccga 
      ggaaaaaaa ccgttgtat ccagaacct atcaatgaa 
      acaaactga ggagatgaa ccatgccgt ggacaggct 
      ggaacagga catgaccga gtcaaaaat caacgaatt 
      agtggcgcg acactacga ggcccattc gctactcaa 
      agtcaaact aaccagcga gttagacac aatcaagga 
      ggcccaaag gagggagta ccgggaatg gctgatgca 
      aatgctgga gcaaaacca agcgacgag ttcactacc 
      aactcccaa ctcgccttt aacgccaca tacccccca 
      acccgcgat ttattcgtc agagtgcgc ccggagcat 
      agacacccc cattttgga ggagagttt caccattca 
      tttggggtc tcagctgaa acagatgca caatatccg 
      cattttgtc ggcaaatgt catggagtt gtgtgccgt 
      ggaagacaa cgatcagtc gtctgagcc aaaaccaca 
      acttctaca aaccgccct ggctttgta ctcaagcga 
      tctctcggg gcttgttct catgactga caataaaat 
      tgggtcacg actcaccca agactttca agaaatttg 
      ccagagaac tactgaatt ccacttccc gcatattga 
      cgaccagtt ggacaaaat ctcgaacac tgctcagct 
      gtacctaaa gtcgcagaa ggagaacag caacagaaa 
      cataaacag cgagttgtt acaagccgg cgattttta 
      catcaccag acattccaa cttagcaca agtccatgt 
      tattttcca tatggtttc ggacgatag cttaagagg 
      cagtgatat taacagcag acatcccgt cgtgttagg 
      gttgaggaa catcctaaa gacggcctg ctccaacga 
      tatcacttc actgaccat cccgttgtt gctacaata 
      cgagatgac agaggaaat gaccatatc atggtgcga 
      aaaacgggc agaactggt ttttaagtg cgtcaaagg 
      tttcatgat tgaaatggc ctcatgggg cggctccga 
      gctgaaaaa cctacagtt tatgctgcc gcaaggcat 
      ctcgagcag agttttcca gtcgctaac cgaaatgtt 
      accaagaat atttaaagt atcaaatcc caaaatatt 
      gaagtaaat taaccatgt gatatgtag gaaaataag 
      aacttttat attatcaat attaattga ataaatatt 
      tcaagttac a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY