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Gene information
General information
TC004125 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1627260..1628707
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004125
    iBeetle screen iB_06567

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004125 RA
      MWRESASLI LAAKSVFGA PNPFNYKIL CLKRSSKSG 
FMPNTYVFP GGNVSPSDS NLEWLKLYE NFGFCKQSF 
DDLRAVENI PNVLEHDEK NRLPKYLSF RICAIRETF 
EECGILLAR PKINADTAS NWASFIGGK ELVSWQHKV 
HDDSEQFFK MCSHFELCP DVWALKNWS HWVTPPSYL 
TSKFNTIFF LTTLHQMPT VHKDEREIQ DLMWTTPEQ 
CIEMNNQQK VLLPVPQFY EMSRLKTVP DVEALSDFA 
TKRARAGFE TYWPFRVKT NEGMYTILP GDDLYQKND 
ENNTAIPQV EQLPEARTK NRILHKSKY SNVVQIENF 
RPKCNHIAP VGAHLFNKG Y
    CDS sequence
    • >TC004125 RA
      atgtggcgc gaatctgcg agtttaatt ttggctgca 
aaatcggtc ttcggagcg cccaacccg ttcaattac 
aaaatcttg tgcctgaaa cgcagttcc aagtcggga 
tttatgcca aacacttac gtttttccc ggaggaaat 
gtatctccg agcgacagc aatttggag tggctaaaa 
ttatacgaa aacttcggt ttttgtaaa caatcattt 
gatgatcta cgagccgtt gaaaacatc ccaaatgta 
ttagaacac gacgagaaa aaccgcctg ccaaaatat 
ttatcgttc aggatttgt gctatcaga gagactttt 
gaagaatgt ggtattttg ctggctcgg cccaaaata 
aacgccgac actgcgtcc aactgggcc tcatttatc 
ggagggaaa gaattggtc tcgtggcag cataaagtg 
cacgacgac tcggaacaa ttttttaaa atgtgttcg 
cactttgaa ttgtgcccc gacgtttgg gcgctgaag 
aactggagc cattgggtc actcctccc tcgtacttg 
acgtccaaa tttaataca atatttttt ctaacaacg 
ctgcaccaa atgccaact gtgcacaaa gatgaaagg 
gaaatccaa gacttaatg tggaccaca ccagagcaa 
tgtatcgag atgaacaac cagcagaaa gttttactg 
ccagtgccg caattttac gaaatgtcg cggttgaaa 
accgtgccg gatgttgag gccctctca gattttgcc 
acaaagcgg gccagagcg ggctttgag acttactgg 
ccctttagg gtcaaaaca aacgaaggg atgtacacc 
atcctgcca ggtgacgat ttgtaccaa aaaaatgac 
gaaaataac actgccatt ccccaagtt gagcagctt 
ccagaagcg cggacaaaa aacaggata ttgcacaag 
tcgaagtac tccaatgtg gttcaaatc gagaatttt 
aggcccaag tgtaatcac atcgcacct gtcggtgcg 
cacttattt aataaaggg tattag
    mRNA sequence
    • >TC004125 RA
      tggccattt cgctcattt aaaatgcaa acaaacact 
tatcataat acgattaat tatcactaa cacagatta 
cttcacatg ttctacgga ccgggcatt gataacaac 
attttgaca taatttctt caaggacgg gagtttcgc 
cggggtcat gaccggcaa tttgaaaat gtggcgcga 
atctgcgag tttaatttt ggctgcaaa atcggtctt 
cggagcgcc caacccgtt caattacaa aatcttgtg 
cctgaaacg cagttccaa gtcgggatt tatgccaaa 
cacttacgt ttttcccgg aggaaatgt atctccgag 
cgacagcaa tttggagtg gctaaaatt atacgaaaa 
cttcggttt ttgtaaaca atcatttga tgatctacg 
agccgttga aaacatccc aaatgtatt agaacacga 
cgagaaaaa ccgcctgcc aaaatattt atcgttcag 
gatttgtgc tatcagaga gacttttga agaatgtgg 
tattttgct ggctcggcc caaaataaa cgccgacac 
tgcgtccaa ctgggcctc atttatcgg agggaaaga 
attggtctc gtggcagca taaagtgca cgacgactc 
ggaacaatt ttttaaaat gtgttcgca ctttgaatt 
gtgccccga cgtttgggc gctgaagaa ctggagcca 
ttgggtcac tcctccctc gtacttgac gtccaaatt 
taatacaat attttttct aacaacgct gcaccaaat 
gccaactgt gcacaaaga tgaaaggga aatccaaga 
cttaatgtg gaccacacc agagcaatg tatcgagat 
gaacaacca gcagaaagt tttactgcc agtgccgca 
attttacga aatgtcgcg gttgaaaac cgtgccgga 
tgttgaggc cctctcaga ttttgccac aaagcgggc 
cagagcggg ctttgagac ttactggcc ctttagggt 
caaaacaaa cgaagggat gtacaccat cctgccagg 
tgacgattt gtaccaaaa aaatgacga aaataacac 
tgccattcc ccaagttga gcagcttcc agaagcgcg 
gacaaaaaa caggatatt gcacaagtc gaagtactc 
caatgtggt tcaaatcga gaattttag gcccaagtg 
taatcacat cgcacctgt cggtgcgca cttatttaa 
taaagggta ttagttatg cttgtctta ttaattcaa 
gcggaacgt tttgttacg gcggattta tggaaggat 
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