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Gene information
General information
TC004131 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1486090..1488906
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004131
      iBeetle screen iB_06565

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
      Days post injection


      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004131 RA
        MSSLKLQKR LAASVMRCG KKKVWLDPN 
        EINEIANTN SRQNIRKLI KDGLIIKKP 
        VAVHSRARV RKNTEARRK GRHCGFGKR 
        KGTANARMP QKELWIQRM RVLRRLLKK 
        YREAKKIDR HLYHALYMK AKGNVFKNK 
        RVLMEYIHK KKAEKARAK MLNDQADAR 
        RLKVKEARK RREERIAAK KLEVLQNYQ 
        REDEATAAK K
      CDS sequence
      • >TC004131 RA
        atgagttcc ctaaaactc caaaaacgc ctagcggcg 
        tctgttatg agatgtggc aagaaaaaa gtgtggctt 
        gatccgaac gaaatcaac gaaattgcc aacaccaac 
        tcaagacaa aacatccgc aagttaatt aaggatggc 
        cttatcatc aaaaagccc gtggctgtg cactcccgc 
        gcccgcgtg cgaaaaaac acggaggcc cggcgaaaa 
        ggcaggcac tgtggtttt ggcaaaaga aagggcact 
        gccaatgcg cgtatgccc caaaaagag ctttggata 
        caacggatg cgtgttttg cgacgcctc ctcaaaaag 
        tacagggag gcgaaaaaa atcgaccga catttgtac 
        cacgctctg tacatgaag gcgaaaggt aacgtcttc 
        aagaacaag agggtcctc atggagtac atccataag 
        aaaaaggcc gagaaagct agggcaaag atgctgaac 
        gaccaagct gacgcgcga cgactgaaa gtcaaggag 
        gcaagaaaa cggagggag gaacgcatc gccgccaaa 
        aagttggaa gttttgcag aattatcaa cgcgaggat 
        gaagcaaca gctgctaaa aaatag
      mRNA sequence
      • >TC004131 RA
        atcgacttt ggtttgtca cactgcaag tgtctcctg 
        ccgctcccc gttaagaca tgaacgctg atcacgaac 
        agcagcaac aagcagagt tggcagtaa aagaggcac 
        gtcgtgaag agcgtcgta aaaaaaaga ggaggagga 
        ggagaagac gacaaaaag aaaaaatgc agcacctaa 
        aacaacttt acgagggga ataacacac cgcaggaga 
        aacaaaacg aagacaccc caacccaat cacaatgaa 
        aaaaagctt ctcatataa tatcaatgt aaaactatc 
        aaatatttt ttaatcagg cacgttgta gattagtag 
        attggttgc acaattagt tcaatataa ccaaatatt 
        tagtgtact ttttatatt atgttatat tgtattact 
        ctattttta ttataaata taaaatatc caatacgac 
        ggacactta ttttaaaga aaattatca aaaattata 
        aaaaatacc gcaacaaca tagataaat taaaaaaca 
        tgaccgtgt atttttaaa ataaaatga aaaagcctc 
        tattttcgt cttaatttt ttttattaa aaaacggtt 
        tggaatttc ttttattga ttttttggg accagaaaa 
        aaattaacc cttagaact tcaaattca aaaaaaggt 
        caagtgaca gttgagtac agcgccact agttttttt 
        gacagttga cacgtgcat agggccata aggttgtgt 
        aagaatgag ttccctaaa actccaaaa acgcctagc 
        ggcgtctgt tatgagatg tggcaagaa aaaagtgtg 
        gcttgatcc gaacgaaat caacgaaat tgccaacac 
        caactcaag acaaaacat ccgcaagtt aattaagga 
        tggccttat catcaaaaa gcccgtggc tgtgcactc 
        ccgcgcccg cgtgcgaaa aaacacgga ggcccggcg 
        aaaaggcag gcactgtgg ttttggcaa aagaaaggg 
        cactgccaa tgcgcgtat gccccaaaa agagctttg 
        gatacaacg gatgcgtgt tttgcgacg cctcctcaa 
        aaagtacag ggaggcgaa aaaaatcga ccgacattt 
        gtaccacgc tctgtacat gaaggcgaa aggtaacgt 
        cttcaagaa caagagggt cctcatgga gtacatcca 
        taagaaaaa ggccgagaa agctagggc aaagatgct 
        gaacgacca agctgacgc gcgacgact gaaagtcaa 
        ggaggcaag aaaacggag ggaggaacg catcgccgc 
        caaaaagtt ggaagtttt gcagaatta tcaacgcga 
        ggatgaagc aacagctgc taaaaaata gttttggga 
        ttttttgtg atggaatgc aattacaga taaatatta 
        caaaacagt cggactttt tctttgaca agcacccaa 
        gcggctaag ttaataagt tacctataa atataataa 
        tcatcatca t
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY