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Gene information
General information
TC004132 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1443599..1449246
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004132
      iBeetle screen iB_06564

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Fertility
      ovary without detectable defects - Number of animals showing the phenotype: 3
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004132 RA
        MLESQDLKL FSIHFWYEK FQSVALKTI LIEIPPILL 
        QKLQSDESD VNIEEICPH EFVTEVKNA INTLDKNVF 
        VKNNWHAPM DAKMFSFGN QLKVTNLDD LSLFLSASN 
        IISEDFSTQ SSIPFYIAL KPWKSIHPA SEFRCIVVN 
        KVLRGITPR DWPTYYAHF KDEGPVIIE TLSNFYKEN 
        IADVFPKRH YTFDVIISF PDQPCIIDF GPLNSKTNL 
        YAFSWKEIS SLFDKEVPE EVAPIFRYL DSDIGIMSR 
        TDAIYKFAN AFT
      CDS sequence
      • >TC004132 RA
        atgttggaa tcgcaagat ttgaaactt ttctcgatt 
        catttttgg tacgaaaag ttccaatct gtcgcgctc 
        aaaacaatt ctaatcgaa atcccgccg attttgttg 
        caaaaactc cagtcagat gagtccgat gtaaacatc 
        gaagaaatc tgtccgcac gaattcgtg actgaggta 
        aaaaatgca attaacact ttagataag aacgttttt 
        gttaaaaac aactggcat gcacctatg gatgctaaa 
        atgttcagt tttgggaac cagttgaaa gttacaaat 
        ttagatgac ttgtcgttg ttcctctcc gcctcaaat 
        attatttca gaggacttt tcaacccaa agcagtatt 
        cccttttat atagcgtta aagccctgg aaaagtatt 
        caccccgct agcgaattc cgatgcatt gtcgtcaac 
        aaagtctta agaggtata acgcctaga gattggcca 
        acgtattat gcccatttc aaagatgag ggtcctgtg 
        attattgag actcttagc aatttttat aaggaaaat 
        attgcggac gtgttccca aaaagacac tatactttc 
        gacgtgata atatcgttt ccggaccaa ccatgtatt 
        attgatttt gggccatta aacagtaaa acgaattta 
        tatgcattt tcttggaaa gaaatttct tctctgttt 
        gataaggaa gtaccagaa gaagtcgct ccaatcttc 
        agatatttg gactctgat attggcatc atgtcaaga 
        actgatgca atttacaaa tttgcgaac gcatttact 
        taa
      mRNA sequence
      • >TC004132 RA
        ttttaaagt tttttgcta aaaattaag gaatccgta 
        cacaagcgc gacatcttt cgagtacgt cttgttatt 
        attgttatt gttatcatt tttttgtgt aactgataa 
        aaatgaagt gaatgaaat aattcatct ttggacact 
        ggacgtatc aagcgccaa aaattgacc atccgaaac 
        agtgaggtt atgagtcac accatgttg gaatcgcaa 
        gatttgaaa cttttctcg attcatttt tggtacgaa 
        aagttccaa tctgtcgcg ctcaaaaca attctaatc 
        gaaatcccg ccgattttg ttgcaaaaa ctccagtca 
        gatgagtcc gatgtaaac atcgaagaa atctgtccg 
        cacgaattc gtgactgag gtaaaaaat gcaattaac 
        actttagat aagaacgtt tttgttaaa aacaactgg 
        catgcacct atggatgct aaaatgttc agttttggg 
        aaccagttg aaagttaca aatttagat gacttgtcg 
        ttgttcctc tccgcctca aatattatt tcagaggac 
        ttttcaacc caaagcagt attcccttt tatatagcg 
        ttaaagccc tggaaaagt attcacccc gctagcgaa 
        ttccgatgc attgtcgtc aacaaagtc ttaagaggt 
        ataacgcct agagattgg ccaacgtat tatgcccat 
        ttcaaagat gagggtcct gtgattatt gagactctt 
        agcaatttt tataaggaa aatattgcg gacgtgttc 
        ccaaaaaga cactatact ttcgacgtg ataatatcg 
        tttccggac caaccatgt attattgat tttgggcca 
        ttaaacagt aaaacgaat ttatatgca ttttcttgg 
        aaagaaatt tcttctctg tttgataag gaagtacca 
        gaagaagtc gctccaatc ttcagatat ttggactct 
        gatattggc atcatgtca agaactgat gcaatttac 
        aaatttgcg aacgcattt acttaagtc ttgttgtca 
        aacagcgat tttttttaa ttgaataaa agttttttt 
        tctta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY