Status
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Gene information
General information
TC001639 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1180461..1189182
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001639
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06563

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_02942

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001639 RA
      MDDGSSKRQ ATRVFKKSS PNGKITVYL GKRDFVDHI 
      DHVDPIDGV VLIDPDYVK DRKVFGHVL AAFRYGRED 
      LDVLGLTFR KDLYLAAEQ IYPPESKNK RELTRLQER 
      LIKKLGPNA YPFYFELPP HCPASVTLQ PAPGDTGKP 
      CGVDYELKA FVGESQDDK PHKRNSVRL AIRKIMYAP 
      SKQGEQPSV EVSKEFMMS PNKLHLEAS LDKELYHHG 
      ENIAVNVHI ANNSNRSVK KIKVSVRQF ADICLFSTA 
      QYKCIVAET ESEEGCPIG PGFTLSKVF TLTPLLANN 
      KDKWGLALD GQLKHEDTN LASSTLVAD PSQRENLGI 
      IVQYKVKVK LCLGALGGD LVAELPFIL MHPKPEEET 
      PPPTSGSRR SSKDVGADV PVDANLIQL DADENNLQD 
      HDDDIIFED FARLRLKGG ETDA
    CDS sequence
    • >TC001639 RA
      atggacgat ggcagcagt aagagacaa gccacaagg 
      gtttttaag aaaagttct cccaatgga aagataacg 
      gtgtattta gggaaacgg gatttcgtg gatcatata 
      gaccacgtg gaccctata gatggtgtt gttctcatc 
      gatcccgat tatgtaaaa gataggaag gtttttggc 
      catgtcctg gctgcgttc aggtatggc cgagaagac 
      ttagacgtt ttaggttta acgtttcgt aaagacctg 
      tatttagca gctgaacag atttatcca ccggaaagt 
      aaaaacaaa agagagttg actaggcta caggaacgc 
      ttaattaaa aaattggga ccaaacgct tatcctttt 
      tatttcgaa ttgccgcca cattgcccc gcatctgta 
      acactccag cctgctcca ggcgatacg ggcaagcct 
      tgtggcgtc gattatgaa cttaaagcg tttgttggc 
      gaatctcaa gatgacaaa ccccacaaa agaaattcc 
      gttcgattg gcaattcgt aagataatg tatgcaccg 
      agtaaacag ggagaacag ccgtcagtt gaagttagt 
      aaggagttt atgatgagt ccgaataaa ttacattta 
      gaagcatcg ctagacaag gaactctac caccatggg 
      gaaaacatc gccgtcaat gtccacata gccaacaat 
      tccaatcgg tccgttaag aaaattaaa gtttccgtt 
      cggcaattt gccgacatt tgccttttt tctaccgct 
      cagtataaa tgtatagtt gccgaaacg gaaagcgaa 
      gaaggctgt cccatcggc ccaggcttc actttgagc 
      aaagtgttc actctaaca cctcttctg gccaataat 
      aaggataag tggggcctc gccttggac ggacaatta 
      aagcacgaa gatactaac ttagcatcg agtacttta 
      gttgctgat ccgtctcaa agagagaat ttgggaata 
      attgtccag tataaagta aaagtaaag ttatgttta 
      ggagctttg ggaggggac ttagtagcc gaacttcct 
      tttatttta atgcacccc aaacctgag gaagaaaca 
      ccacctccg acttccggc tcgcgtcgt tctagcaaa 
      gatgtgggg gccgatgtt ccggtcgac gccaatttg 
      atccagctg gacgcggac gaaaataat ttgcaagat 
      cacgacgac gacattatt ttcgaagat ttcgctcgg 
      cttcgatta aaaggaggt gagactgac gcatga
    mRNA sequence
    • >TC001639 RA
      gttggctac aagttcaaa atctggttt tgtttccgc 
      agtctttgg tcaaaattt tggtttttt gcttggagg 
      cggcccttt cgggcgccg gggccgggg ccccacgtc 
      gtggcggcg tcctgggtg tgcgtgccc atgatggtc 
      ggttggcag gtctgaatc agcatgcgc ttcagttgc 
      tttatatcc ctactccac tccccagtt ttcacagtt 
      gggaagttg gacaaggcc caaggtgtt ccaatggga 
      agaccatcg aattgtttg gggttgtga aatcgtaaa 
      tttgtggtt attcaccgg ttggtcccg gtgcccgaa 
      gtccaccct ccctggggc gcctcctcg catagtgca 
      gttctgttg gtgtgcatt ggacgtgta gagttaaat 
      tttaggcac ttccaggaa aatactgta cacatcaga 
      ctggagggg cacaacgtg ccctcgctt tgtcaacca 
      gaccctggc atcacgttg gctccttct agcccagat 
      atggacgat ggcagcagt aagagacaa gccacaagg 
      gtttttaag aaaagttct cccaatgga aagataacg 
      gtgtattta gggaaacgg gatttcgtg gatcatata 
      gaccacgtg gaccctata gatggtgtt gttctcatc 
      gatcccgat tatgtaaaa gataggaag gtttttggc 
      catgtcctg gctgcgttc aggtatggc cgagaagac 
      ttagacgtt ttaggttta acgtttcgt aaagacctg 
      tatttagca gctgaacag atttatcca ccggaaagt 
      aaaaacaaa agagagttg actaggcta caggaacgc 
      ttaattaaa aaattggga ccaaacgct tatcctttt 
      tatttcgaa ttgccgcca cattgcccc gcatctgta 
      acactccag cctgctcca ggcgatacg ggcaagcct 
      tgtggcgtc gattatgaa cttaaagcg tttgttggc 
      gaatctcaa gatgacaaa ccccacaaa agaaattcc 
      gttcgattg gcaattcgt aagataatg tatgcaccg 
      agtaaacag ggagaacag ccgtcagtt gaagttagt 
      aaggagttt atgatgagt ccgaataaa ttacattta 
      gaagcatcg ctagacaag gaactctac caccatggg 
      gaaaacatc gccgtcaat gtccacata gccaacaat 
      tccaatcgg tccgttaag aaaattaaa gtttccgtt 
      cggcaattt gccgacatt tgccttttt tctaccgct 
      cagtataaa tgtatagtt gccgaaacg gaaagcgaa 
      gaaggctgt cccatcggc ccaggcttc actttgagc 
      aaagtgttc actctaaca cctcttctg gccaataat 
      aaggataag tggggcctc gccttggac ggacaatta 
      aagcacgaa gatactaac ttagcatcg agtacttta 
      gttgctgat ccgtctcaa agagagaat ttgggaata 
      attgtccag tataaagta aaagtaaag ttatgttta 
      ggagctttg ggaggggac ttagtagcc gaacttcct 
      tttatttta atgcacccc aaacctgag gaagaaaca 
      ccacctccg acttccggc tcgcgtcgt tctagcaaa 
      gatgtgggg gccgatgtt ccggtcgac gccaatttg 
      atccagctg gacgcggac gaaaataat ttgcaagat 
      cacgacgac gacattatt ttcgaagat ttcgctcgg 
      cttcgatta aaaggaggt gagactgac gcatgaaag 
      aaggtacag gctcaggct atgcagcct gcccgtcca 
      ttccaaccc atacgtact gatgtgtat atcagctgc 
      gattaggtg tgaacccat gatccaagt gtgtgccct 
      agtcatcgg ctgtgctgc cagtcacaa ccagaatgc 
      acccattta tctcagggc aggggtttt tgacgtact 
      tttgcattt tgggcactc gtagtttta ttgctttga 
      agttttatt tcaaatgga atactagtc aagtgtttg 
      agtgccgcg attaaaagc ccctgcgct caacttact 
      ttcttcaac ctctgtaaa ataaaacca aatcttgct 
      tatctgaaa atcaaactt cagttctta tgatttttt 
      aaccttctg tacctagag agtgaattc tatacctat 
      tatcagaag cgcagagtc ttggctaag cgattagtt 
      ggcgttttg tacgtttgc attcattta gtggtgtta 
      gtgttttaa aattgtgca caattcaac tggctgtat 
      ttatttcct ttgtaaata atgtttccg ttttcgtct 
      gtctctcgc tcttataat gggtgtaaa agtttaaat 
      tactcgcag aaaataata aagtaacgc tacatacta 
      attttacaa ttaaatgat aactttgta ctgaattta 
      attaataag tattttgta ttacttttt taaagcact 
      tttatactc aaatataaa gtactgact atcggttgt 
      agtcataaa tatgtaaaa ggctgataa cagattaat 
      atatttttg tgtcagagt tggattgga tgaatgatc 
      agcgcggtt atgattaca accgtagat tttttgggt 
      agttagtta agtgctact gttacctaa atgcagttc 
      aacacaaac gtttttagg gttaatgtt cgtaaaaat 
      gtatgtatg ttcctctct ttctgtata cacaagctt 
      tcttcaaaa ttatttatg tgctatctt tgctgtttt 
      gtatgttgt cttagcatt tgatcgaac tcatgcatt 
      tatttgaac gccataagg agaaaatat ttatcacta 
      cgcaagaac attgtttcg tgcataagc atattagat 
      gatttgcta caaaaacag tatttgtaa ccacttgtg 
      atactttaa ttttacgct caattcgac tattacaga 
      tttatttct aaccatatc agtgtaaca atgtatttt 
      ttcacacac gttgtgcct tgtgataaa tgtattttc 
      ttcacatta gtaaatcat cgttctttt tctgaccat 
      catgaaatg tgaccgcaa actgtctgt tctatctat 
      acatgtcct tatttttgt tactttcgg tggaaaatt 
      gattttcga agtatacta gagttgcat ttttcgttc 
      tttaagttc atgtaatat tctgtaaaa taattgtgt 
      atactaagt aaataaaat aaattagga gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY