Status
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Gene information
General information
TC001604 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1251761..1257475
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001604
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06561

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001604 RA
      MESFAFAIK AKIELWLKY LVGSGGLTM SVSGSLEYL 
      MIKQTCRFF AYKFEMGSS QSPQDASDI YSFEFLKAL 
      YDRPRDNIN RIIKASFGG ENGDSEAVK ANDENYTLI 
      EKRVLLDFF KKVVDMKSQ PAKQWTDLQ HYLCFIYRY 
      CMTTDPDPV QEALSPYFI ADMRSIVCS LMEEDQIQL 
      CLRLFSLVR EYTVFAHKQ HQKIASVEA SIESLFYYY 
      EAFLSATKN LCKIFPELE QLLINTYKL GWLDFNTSI 
      FMYYFYETP YVQDMVRKF EMIKDNVSI KAAGIYTVL 
      TELRKLWRS QMLGINSVI ANKLGARFK GLDDKMMAS 
      HKRVLAKNF WIDRTAKEK AMQDVRLFD CQLFTLVGN 
      KNLPMPDPT LDESGQCIC EDCLIIKYK SLLENTQNK 
      IASECMKVS CRFCRQQVD LEHFQQHVN GRAVAELDG 
      NNCKNLSRI ISDNIDIVK LGISGSRKN GAHQNDSLI 
      SKSLKRHEN TNGEISKDP CPKLANEPP LFKHNIFED 
      EDEDIDVTE LAFPGLEIT KMSRKTFYN FLKHRFALP 
      RNGKENKAE TSPKVAEKA PVVQNAVKN TPPKPEETK 
      GSEVVKNSK NTNKTNGNT FPCGHSCKL EEELKSEFK 
      KAYQDQCDH HKESKKCDC TFCEVFGTT TSHVHKTNE 
      TRNRLRIRL NQRNESGSA KNKKTAPKA SATKVESPA 
      GPSKQNQSS EGTTPTNEE TNSNPLMND IHGLLNYIE 
      GNTNVDKVA LAEKKAAKK ARQRHKKEE ERLKLEEEE 
      RKRVEEEKR KAEERLRNE ELARQLAEK AAKNSKKEN 
      KKKKQKNKG KDGSPELVE ETIPAMVTI KRVVENDNA 
      LPTVTITLK GSTPDQDKL LYTLVNGHD GNNAQLEEN 
      KKIKKNKEL VAKTVNLEV AANQKKKKN KNEKNNQPA 
      NAVVTKELR VTLAVDPPS DKKNKQTKK TENKKDKIN 
      NKNGDINIP MLKLPPGIT ITKVDGPMT DRNYKATNI 
      ESPPYSSNI PVSKSGVIV VDTEKLIKQ SNVTTNGTT 
      TASKKNRRK RNKKQQLQT APQTAPSKP SMVTLKNPI 
      FQNMQPRHE PEIMPDMSG GQCQQASIF KNENGMVTI 
      RSSRLQQSL NSGAPMPSL LPDLKPVLG PEVPTSYMS 
      SCNFPEFSR ISPFNAQEI LSGLPGIEI TKVDKNATR 
      SDLDSKKAC HTAEVSIIP ANNSDKFNF DKDDLHYDN 
      VFTPKDILE DDMDADERE LEAFKRFCQ QSVPPKRKE 
      KVAHLNVKD IVLKKKNEK ITA
    CDS sequence
    • >TC001604 RA
      atggagtcg tttgcgttt gccataaaa gcaaaaatt 
      gaattgtgg ctgaaatac ctggtggga agtggtggt 
      ttaaccatg agtgtgtcg ggttctctt gagtatctc 
      atgataaag cagacttgt cggttcttc gcttacaag 
      tttgaaatg ggctcaagt caatcgccc caagacgca 
      tcggacatc tactcattc gagttttta aaggcctta 
      tacgaccgc ccgagggac aacattaat aggataatt 
      aaggcgagc ttcgggggc gagaatggc gacagcgaa 
      gctgtcaag gcaaacgac gaaaactat accctaatc 
      gaaaagagg gtgctgtta gacttcttc aagaaagtg 
      gtcgatatg aagtcacaa ccggccaag cagtggaca 
      gatttgcag cactacttg tgcttcatt tataggtat 
      tgcatgacc acggacccg gacccggtg caggaggcg 
      ctctcgccc tatttcata gccgacatg aggagcatc 
      gtttgcagt ctgatggag gaagaccag atccaactt 
      tgccttaga ttattttcc cttgtgcga gaatacacc 
      gtttttgct cacaagcaa caccaaaaa atcgcaagc 
      gtggaggct tccattgaa agcttgttc tactactac 
      gaggcgttt ctgtccgca actaaaaac ctgtgcaag 
      attttcccc gagttggag caactccta atcaacacc 
      tacaagttg ggctggctt gactttaac acctcgatt 
      tttatgtac tatttttac gaaacgccc tacgtccaa 
      gacatggtg cgcaaattc gaaatgatc aaagacaac 
      gtctcgatt aaagcggcc gggatttac accgtttta 
      accgaactg cgcaagctg tggaggtcg caaatgttg 
      ggaattaac agcgttatt gccaacaaa cttggcgct 
      aggtttaaa ggcctggat gataagatg atggcgagt 
      cataaacgc gtcttagcg aagaacttt tggatagat 
      cggacggca aaagagaaa gcaatgcag gacgtgaga 
      ctcttcgac tgccaattg ttcacactt gtggggaac 
      aaaaacctg cctatgccc gacccgact ttggacgag 
      tcaggccaa tgcatctgc gaggactgc ttaataatc 
      aaatacaag tcactgctt gaaaatact cagaacaaa 
      atcgcctcc gagtgcatg aaagtctct tgcagattc 
      tgccgccaa caagtcgat ttagagcac ttccaacag 
      cacgtcaac ggccgtgct gtggcggaa ctcgacggc 
      aacaactgt aaaaatctc tcgcggatc atctccgac 
      aacatcgat atcgtcaaa ctcggaata tcaggctcg 
      cggaaaaac ggggcgcac caaaacgac tcactcata 
      tcaaagtct ctcaaacga cacgaaaac accaacgga 
      gaaatttcc aaagatccg tgccccaaa ctcgccaac 
      gaaccccca ctcttcaaa cacaacatt ttcgaagac 
      gaggacgaa gatatcgat gttacggag ttagcgttc 
      ccgggacta gaaatcacg aaaatgtcg cgtaaaacg 
      ttttacaat tttctgaaa cacaggttt gccttgcct 
      cgtaatggc aaggagaac aaagctgag actagtccg 
      aaagtcgcg gaaaaagca ccagttgtg caaaatgcg 
      gtcaaaaat acgccgccg aaacccgaa gaaacgaag 
      gggtctgag gtggtcaaa aactcgaag aatacgaac 
      aaaactaac ggtaacacc ttcccgtgc ggtcacagt 
      tgcaaactt gaggaggaa ttaaaatca gagtttaaa 
      aaagcttat caggaccag tgcgaccac cacaaggag 
      tcgaagaaa tgcgattgc actttttgc gaagtgttc 
      gggacaacg acttctcac gttcacaaa acgaacgaa 
      acccggaac aggttacga atcagattg aaccagagg 
      aatgaaagc ggtagcgcg aagaacaag aaaaccgct 
      ccgaaagcg agcgcgacc aaggtggaa tcaccggct 
      gggccttcg aaacaaaac caatcgagc gaagggacg 
      actccgacc aatgaagaa acgaactcc aatccgtta 
      atgaacgac attcacgga ctcctcaac tatatcgag 
      ggcaacacg aatgtggac aaagtggct ttggcggag 
      aagaaagcg gcgaagaag gccaggcaa aggcacaaa 
      aaagaggag gaacggttg aagcttgag gaggaggaa 
      cggaagcga gttgaggag gaaaaacgc aaagctgag 
      gagcgactc cggaatgag gagttagca agacagctt 
      gcagaaaaa gcggccaaa aactcgaag aaagaaaat 
      aagaagaag aaacagaag aacaaaggc aaggacggc 
      agtcctgag ttggtcgaa gagacaatt ccggccatg 
      gtcactata aaacgtgtc gtcgaaaat gataatgca 
      ctgccaact gttactatc accctaaag ggttcgacg 
      cccgatcaa gacaaactt ttatacacg ctagtgaac 
      ggccatgat ggcaataat gctcagtta gaagagaac 
      aagaagatt aaaaagaac aaagaactc gtggcgaag 
      actgttaat ttagaagtt gcagctaat cagaagaag 
      aaaaagaac aaaaatgaa aaaaacaac cagcccgct 
      aatgcagtt gttacaaaa gaattgcga gtgacacta 
      gccgtagac cctccaagt gataaaaag aacaaacaa 
      acgaagaaa accgaaaat aagaaagac aaaatcaat 
      aacaagaac ggcgatatt aatattccc atgctgaaa 
      ctgccacca ggaattaca ataacgaag gtcgacggc 
      ccaatgacc gaccggaat tacaaggcc acgaacatc 
      gagagcccg ccttacagt tcgaacatc ccggtgagc 
      aaatcggga gtgatagtc gtagacacg gaaaaacta 
      ataaagcaa tcaaacgtg acaacaaac ggcaccaca 
      accgctagc aagaaaaac cggcgaaag cgcaacaaa 
      aaacaacag ttgcagacc gccccccag acagccccg 
      agcaagccg tcgatggtg actttgaaa aacccgatt 
      ttccaaaac atgcagccc cgccacgag ccggaaata 
      atgccggac atgagcggc ggccagtgc caacaagcc 
      tcgattttc aaaaacgag aacgggatg gtcaccatt 
      cggagctcc agactgcaa caaagcctc aacagtggg 
      gcccccatg cccagcctt ttgccagat ctgaagccg 
      gtcctgggg cccgaagtc cccactagc tacatgtcg 
      tcgtgcaac ttcccggag ttctcccgg atttcgccg 
      ttcaacgct caagaaatt ctgtcaggc ttgccgggc 
      atcgagatc acgaaggtg gacaaaaac gcgacacgg 
      agcgatcta gacagcaag aaggcgtgc cacacggcg 
      gaggtctcg atcattccg gcgaacaat tccgacaaa 
      tttaatttt gataaagat gacttgcat tatgataac 
      gttttcacg ccgaaggat attctggag gacgatatg 
      gacgccgac gagcgggag ctggaggcc ttcaagcgg 
      ttctgccag cagtccgtg ccgcccaag cgcaaggaa 
      aaagttgct catctcaat gttaaagat attgtttta 
      aagaagaaa aacgagaaa ataacggct tag
    mRNA sequence
    • >TC001604 RA
      gctgttttg aatttctgg aatggtttc atggagtcg 
      tttgcgttt gccataaaa gcaaaaatt gaattgtgg 
      ctgaaatac ctggtggga agtggtggt ttaaccatg 
      agtgtgtcg ggttctctt gagtatctc atgataaag 
      cagacttgt cggttcttc gcttacaag tttgaaatg 
      ggctcaagt caatcgccc caagacgca tcggacatc 
      tactcattc gagttttta aaggcctta tacgaccgc 
      ccgagggac aacattaat aggataatt aaggcgagc 
      ttcgggggc gagaatggc gacagcgaa gctgtcaag 
      gcaaacgac gaaaactat accctaatc gaaaagagg 
      gtgctgtta gacttcttc aagaaagtg gtcgatatg 
      aagtcacaa ccggccaag cagtggaca gatttgcag 
      cactacttg tgcttcatt tataggtat tgcatgacc 
      acggacccg gacccggtg caggaggcg ctctcgccc 
      tatttcata gccgacatg aggagcatc gtttgcagt 
      ctgatggag gaagaccag atccaactt tgccttaga 
      ttattttcc cttgtgcga gaatacacc gtttttgct 
      cacaagcaa caccaaaaa atcgcaagc gtggaggct 
      tccattgaa agcttgttc tactactac gaggcgttt 
      ctgtccgca actaaaaac ctgtgcaag attttcccc 
      gagttggag caactccta atcaacacc tacaagttg 
      ggctggctt gactttaac acctcgatt tttatgtac 
      tatttttac gaaacgccc tacgtccaa gacatggtg 
      cgcaaattc gaaatgatc aaagacaac gtctcgatt 
      aaagcggcc gggatttac accgtttta accgaactg 
      cgcaagctg tggaggtcg caaatgttg ggaattaac 
      agcgttatt gccaacaaa cttggcgct aggtttaaa 
      ggcctggat gataagatg atggcgagt cataaacgc 
      gtcttagcg aagaacttt tggatagat cggacggca 
      aaagagaaa gcaatgcag gacgtgaga ctcttcgac 
      tgccaattg ttcacactt gtggggaac aaaaacctg 
      cctatgccc gacccgact ttggacgag tcaggccaa 
      tgcatctgc gaggactgc ttaataatc aaatacaag 
      tcactgctt gaaaatact cagaacaaa atcgcctcc 
      gagtgcatg aaagtctct tgcagattc tgccgccaa 
      caagtcgat ttagagcac ttccaacag cacgtcaac 
      ggccgtgct gtggcggaa ctcgacggc aacaactgt 
      aaaaatctc tcgcggatc atctccgac aacatcgat 
      atcgtcaaa ctcggaata tcaggctcg cggaaaaac 
      ggggcgcac caaaacgac tcactcata tcaaagtct 
      ctcaaacga cacgaaaac accaacgga gaaatttcc 
      aaagatccg tgccccaaa ctcgccaac gaaccccca 
      ctcttcaaa cacaacatt ttcgaagac gaggacgaa 
      gatatcgat gttacggag ttagcgttc ccgggacta 
      gaaatcacg aaaatgtcg cgtaaaacg ttttacaat 
      tttctgaaa cacaggttt gccttgcct cgtaatggc 
      aaggagaac aaagctgag actagtccg aaagtcgcg 
      gaaaaagca ccagttgtg caaaatgcg gtcaaaaat 
      acgccgccg aaacccgaa gaaacgaag gggtctgag 
      gtggtcaaa aactcgaag aatacgaac aaaactaac 
      ggtaacacc ttcccgtgc ggtcacagt tgcaaactt 
      gaggaggaa ttaaaatca gagtttaaa aaagcttat 
      caggaccag tgcgaccac cacaaggag tcgaagaaa 
      tgcgattgc actttttgc gaagtgttc gggacaacg 
      acttctcac gttcacaaa acgaacgaa acccggaac 
      aggttacga atcagattg aaccagagg aatgaaagc 
      ggtagcgcg aagaacaag aaaaccgct ccgaaagcg 
      agcgcgacc aaggtggaa tcaccggct gggccttcg 
      aaacaaaac caatcgagc gaagggacg actccgacc 
      aatgaagaa acgaactcc aatccgtta atgaacgac 
      attcacgga ctcctcaac tatatcgag ggcaacacg 
      aatgtggac aaagtggct ttggcggag aagaaagcg 
      gcgaagaag gccaggcaa aggcacaaa aaagaggag 
      gaacggttg aagcttgag gaggaggaa cggaagcga 
      gttgaggag gaaaaacgc aaagctgag gagcgactc 
      cggaatgag gagttagca agacagctt gcagaaaaa 
      gcggccaaa aactcgaag aaagaaaat aagaagaag 
      aaacagaag aacaaaggc aaggacggc agtcctgag 
      ttggtcgaa gagacaatt ccggccatg gtcactata 
      aaacgtgtc gtcgaaaat gataatgca ctgccaact 
      gttactatc accctaaag ggttcgacg cccgatcaa 
      gacaaactt ttatacacg ctagtgaac ggccatgat 
      ggcaataat gctcagtta gaagagaac aagaagatt 
      aaaaagaac aaagaactc gtggcgaag actgttaat 
      ttagaagtt gcagctaat cagaagaag aaaaagaac 
      aaaaatgaa aaaaacaac cagcccgct aatgcagtt 
      gttacaaaa gaattgcga gtgacacta gccgtagac 
      cctccaagt gataaaaag aacaaacaa acgaagaaa 
      accgaaaat aagaaagac aaaatcaat aacaagaac 
      ggcgatatt aatattccc atgctgaaa ctgccacca 
      ggaattaca ataacgaag gtcgacggc ccaatgacc 
      gaccggaat tacaaggcc acgaacatc gagagcccg 
      ccttacagt tcgaacatc ccggtgagc aaatcggga 
      gtgatagtc gtagacacg gaaaaacta ataaagcaa 
      tcaaacgtg acaacaaac ggcaccaca accgctagc 
      aagaaaaac cggcgaaag cgcaacaaa aaacaacag 
      ttgcagacc gccccccag acagccccg agcaagccg 
      tcgatggtg actttgaaa aacccgatt ttccaaaac 
      atgcagccc cgccacgag ccggaaata atgccggac 
      atgagcggc ggccagtgc caacaagcc tcgattttc 
      aaaaacgag aacgggatg gtcaccatt cggagctcc 
      agactgcaa caaagcctc aacagtggg gcccccatg 
      cccagcctt ttgccagat ctgaagccg gtcctgggg 
      cccgaagtc cccactagc tacatgtcg tcgtgcaac 
      ttcccggag ttctcccgg atttcgccg ttcaacgct 
      caagaaatt ctgtcaggc ttgccgggc atcgagatc 
      acgaaggtg gacaaaaac gcgacacgg agcgatcta 
      gacagcaag aaggcgtgc cacacggcg gaggtctcg 
      atcattccg gcgaacaat tccgacaaa tttaatttt 
      gataaagat gacttgcat tatgataac gttttcacg 
      ccgaaggat attctggag gacgatatg gacgccgac 
      gagcgggag ctggaggcc ttcaagcgg ttctgccag 
      cagtccgtg ccgcccaag cgcaaggaa aaagttgct 
      catctcaat gttaaagat attgtttta aagaagaaa 
      aacgagaaa ataacggct tagatttag gttttttat 
      cggaagcgc ggaggacaa ttttatttg gtttacgtt 
      ttattgctt cctttattt atatcactt taagaaatt 
      cccatgtgc agatctaat ctgaatgtt attattagg 
      aaaatgtga ttaattttt tcaccaaat tttgatgtt 
      tctttattg ccagaccag taaactttt aaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY