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Gene information
General information
TC032121
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000975
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9089341..9091577
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032121
    iBeetle screen iB_06560

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    egg chamber shape irregular - Number of animals showing the phenotype: 4

    follicular epithelium potentially not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032121 RA
      MKLLYYNTV VLFLRQYKI ETRPNAGPG FWLMLLLPS 
      ALIISVAQH PIISTSTYK LSSIFSLGL ILTSFTFMH 
      QISQTRLKL LNIWLVTSC LIVAVLFRI CLHRGWPFS 
      VFSAFVATF FYHKLYIYF LQKLPKCFT LGEAAICTQ 
      SYTLLLYFT SVNVWQWVR KPASNVVQM STLTIQLGL 
      LAISTIIFL AYKLRITSS FKFYSLTAA SFVLIVLLP 
      LQFLMRINP LLWILNLIW DDLFANMVV VYWAFFSII 
      AIAAVNLRI KKAQKASTA VRKIFHLLT VAVFIPGLL 
      YNCSFLYLA TGVILGIFF MLEVLRVLN MPPLAEVLQ 
      NGLVVFCDE KDTGIIAFT PMYLLAGCS LPLWIHPSP 
      CDVTNSAVF NLLPLLSGL LTIGIGDTA ASVVGSKFG 
      KFHWPGSRK TIEGTLACI LSQLFVIFA FVYLDYVKL 
      VTSVQYVKT VSAVIVTSI VEAKTDQVD NLVLPFVMY 
      LFFIVV
    CDS sequence
    • >TC032121 RA
      atgaaactc ctgtattat aacaccgtt gtccttttc 
      ttacgccag tacaaaatc gagaccagg ccaaatgcc 
      gggcctggg ttttggctc atgctctta ttgccctcg 
      gccctaatt atttcagtg gcccagcat ccaattatc 
      tcgacttcc acttacaaa ttatcgtcg attttttca 
      ttagggttg attttaacg tcgttcaca tttatgcac 
      caaatatca caaacaaga ctgaaactt ttgaacatt 
      tggcttgtg acaagttgc ctcattgtg gccgtttta 
      tttaggata tgtcttcac agaggatgg ccgttttcg 
      gtttttagt gcctttgtt gcgacgttt ttttaccat 
      aaactctac atttatttt ttacaaaaa ctgccgaaa 
      tgcttcaca ttgggcgaa gctgcgatt tgtactcaa 
      agttacacg cttttgttg tatttcact tcggttaat 
      gtttggcaa tgggtgaga aaaccggcc agtaatgtt 
      gttcaaatg tcgactctc acaattcag ctgggtttg 
      ttggcaata tcgacaatc attttcctg gcgtacaaa 
      ttgagaata acaagtagt tttaaattt tactctcta 
      acagcggca agtttcgtt ttgatcgtt ttattgccg 
      ctacaattt ttaatgcga ataaatccg ttgttatgg 
      attttaaat ttaatttgg gacgacctt tttgcgaac 
      atggttgtc gtttattgg gcttttttc agcattatt 
      gctatagcg gccgtaaat ctccgaata aaaaaggcc 
      caaaaggct tcaactgcc gtacgaaaa atcttccat 
      ttattaaca gtcgccgtt tttatccct ggtctttta 
      tacaactgt tccttcctc tatttagcc acaggggtg 
      atccttgga atttttttc atgctggag gttttgagg 
      gtgttgaac atgccaccc ttggccgag gttttgcaa 
      aacggcctt gttgtgttt tgtgacgaa aaagacacc 
      ggaattata gctttcaca ccgatgtat ctcttggca 
      ggttgttca ctgcctttg tggatccac ccatcgcct 
      tgtgatgtt acaaattcg gcggttttt aatttgttg 
      cctttgttg agtggtttg ttgaccatt gggatcggt 
      gatacggct gcgagtgtc gttggttca aagtttggc 
      aaatttcat tggccaggt tctaggaaa actatagaa 
      gggacactt gcgtgtatt ttgagccaa ctttttgtc 
      atatttgct tttgtctac ttagattac gtaaaattg 
      gtgacttct gtacaatac gtgaagact gtttccgca 
      gtaattgtt acctcgatt gttgaggca aaaactgat 
      caagttgat aatttagtg ctgccattt gtgatgtat 
      ttatttttt attgtggtt tag
    mRNA sequence
    • >TC032121 RA
      agcctttta aataatttt ttgtcgagt aaaacgtac 
      gaactgaac taatctcac aacaaaaaa tcaaagtta 
      attaatttt ttgcccgct tgtttgaca cgtcaggtg 
      tcaaaactg acgtttaat gccaaagtt gaggttata 
      ctaatacaa aataattaa attagcacc ttatttgca 
      ttgattcct tcaaattac gacaaaata taagagttc 
      tggtcgtaa ttaccaatt ccgaaatga ttttggagt 
      gtgtttatg tcacaaatc gcattattt ttcgaatga 
      acattgaac aagactcct ggtcaaagt ttaagtagt 
      gaataaatc gttccgtag tcttgtttt ggaaaaatg 
      aaactcctg tattataac accgttgtc cttttctta 
      cgccagtac aaaatcgag accaggcca aatgccggg 
      cctgggttt tggctcatg ctcttattg ccctcggcc 
      ctaattatt tcagtggcc cagcatcca attatctcg 
      acttccact tacaaatta tcgtcgatt ttttcatta 
      gggttgatt ttaacgtcg ttcacattt atgcaccaa 
      atatcacaa acaagactg aaacttttg aacatttgg 
      cttgtgaca agttgcctc attgtggcc gttttattt 
      aggatatgt cttcacaga ggatggccg ttttcggtt 
      tttagtgcc tttgttgcg acgtttttt taccataaa 
      ctctacatt tatttttta caaaaactg ccgaaatgc 
      ttcacattg ggcgaagct gcgatttgt actcaaagt 
      tacacgctt ttgttgtat ttcacttcg gttaatgtt 
      tggcaatgg gtgagaaaa ccggccagt aatgttgtt 
      caaatgtcg actctcaca attcagctg ggtttgttg 
      gcaatatcg acaatcatt ttcctggcg tacaaattg 
      agaataaca agtagtttt aaattttac tctctaaca 
      gcggcaagt ttcgttttg atcgtttta ttgccgcta 
      caattttta atgcgaata aatccgttg ttatggatt 
      ttaaattta atttgggac gaccttttt gcgaacatg 
      gttgtcgtt tattgggct tttttcagc attattgct 
      atagcggcc gtaaatctc cgaataaaa aaggcccaa 
      aaggcttca actgccgta cgaaaaatc ttccattta 
      ttaacagtc gccgttttt atccctggt cttttatac 
      aactgttcc ttcctctat ttagccaca ggggtgatc 
      cttggaatt tttttcatg ctggaggtt ttgagggtg 
      ttgaacatg ccacccttg gccgaggtt ttgcaaaac 
      ggccttgtt gtgttttgt gacgaaaaa gacaccgga 
      attatagct ttcacaccg atgtatctc ttggcaggt 
      tgttcactg cctttgtgg atccaccca tcgccttgt 
      gatgttaca aattcggcg gtttttaat ttgttgcct 
      ttgttgagt ggtttgttg accattggg atcggtgat 
      acggctgcg agtgtcgtt ggttcaaag tttggcaaa 
      tttcattgg ccaggttct aggaaaact atagaaggg 
      acacttgcg tgtattttg agccaactt tttgtcata 
      tttgctttt gtctactta gattacgta aaattggtg 
      acttctgta caatacgtg aagactgtt tccgcagta 
      attgttacc tcgattgtt gaggcaaaa actgatcaa 
      gttgataat ttagtgctg ccatttgtg atgtattta 
      ttttttatt gtggtttag ttatgtgtc aataaagaa 
      atttttata ttttttttg cccaaaata aaagcctca 
      aaaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY