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Gene information
General information
TC001615 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1342291..1343846
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001615
    iBeetle screen iB_06558

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001615 RA
      MKGFEDNDC RLCSKTFCC PNCRILHEE KFHNVNPEC 
      EICVYGRAS LKNASVSLL NHIKTAHWP LHCVFCKKL 
      FTSFDDLLS HDKCPFKLE TLKMETTPK TPVTSLTET 
      EKPSPEVTP FYKATEHGA LKTRTMGAT STPLIHKDS 
      DKSDKITPI NQSEIIYHK SSLKLTGSS NFSDSKKSD 
      RRVTFSETP FVSEMCKKP KYLPLPKQI TNENSNVSN 
      DDSTDELEL YKTAPHTIL ECEEKENLD NIGVFNNCD 
      ANNDGTLWE SALTKLDFS GCNNIIEKI DNKTNNFNV 
      SLQINVFSP PNSPKSKSN DADLSLNKS TNIWSSVTS 
      MVKSVVSNI SMTAQSSFS GVGLKRPQP DNDALCDAP 
      HLKRMKLTD IKCRRPIRT ISPLYQIRL ARPRYVDKA 
      TQTD
    CDS sequence
    • >TC001615 RA
      atgaaaggc tttgaagac aacgactgt cgcttgtgt 
      tcaaaaaca ttctgttgc ccgaactgc cgcatctta 
      cacgaggaa aaattccac aacgtgaac ccggagtgt 
      gaaatttgc gtttacggc cgggcttcc cttaaaaac 
      gcctcagtt tcgctacta aaccacata aaaaccgcc 
      cattggccc ttgcattgt gttttttgt aaaaaactg 
      tttacttct tttgacgac cttctctcg catgataaa 
      tgtcctttt aagcttgaa acgctcaaa atggaaaca 
      actccaaaa actccagtc acttcactg acagaaacc 
      gaaaagccc tcgcctgaa gtaaccccc ttttacaag 
      gcgactgaa cacggggcc ctcaaaacc aggaccatg 
      ggggccacg agcacgccc ctgattcac aaagatagc 
      gacaaaagc gataaaatc accccgatt aatcagagc 
      gaaattatc taccataaa tcaagcttg aagctaaca 
      gggtcgtcg aatttcagc gactccaag aaaagcgac 
      cgcagggtc actttttcg gaaaccccc tttgtgagt 
      gaaatgtgc aaaaagccc aagtattta cctttgcct 
      aaacaaata acgaatgaa aattcaaac gtttccaat 
      gacgactca accgatgaa ctggaattg tacaaaaca 
      gcccctcac acaattcta gaatgcgaa gaaaaggag 
      aatttagat aacattggc gtatttaat aattgtgac 
      gctaataac gacgggacc ttatgggaa agtgcttta 
      acaaaattg gatttcagc ggctgtaat aacatcatt 
      gagaagatt gacaacaaa acgaataat tttaacgtt 
      tcgctgcaa ataaatgtg ttcagcccg ccaaatagt 
      ccaaaatcg aaaagcaac gacgctgac ttgtctttg 
      aataaatcc acaaacatt tggtcctct gtgacaagc 
      atggtcaag tcggtcgtg agcaacatc tcaatgacg 
      gcgcagagt agtttttca ggcgtaggt ctcaaaagg 
      ccgcagcct gataatgac gcactttgc gacgcccca 
      catttgaag agaatgaaa ctgaccgac attaagtgc 
      cgacggcca atcaggacc ataagtccc ttgtatcaa 
      ataagactc gcacgcccc cgttatgta gacaaagcg 
      actcaaacc gactaa
    mRNA sequence
    • >TC001615 RA
      atttttgtt gtgcgagtt gtggaaaaa aaaatcaaa 
      agttaccga ctgatttgg cgggttttt atggcggca 
      atttgaaaa aatgaaagg ctttgaaga caacgactg 
      tcgcttgtg ttcaaaaac attctgttg cccgaactg 
      ccgcatctt acacgagga aaaattcca caacgtgaa 
      cccggagtg tgaaatttg cgtttacgg ccgggcttc 
      ccttaaaaa cgcctcagt ttcgctact aaaccacat 
      aaaaaccgc ccattggcc cttgcattg tgttttttg 
      taaaaaact gtttacttc ttttgacga ccttctctc 
      gcatgataa atgtccttt taagcttga aacgctcaa 
      aatggaaac aactccaaa aactccagt cacttcact 
      gacagaaac cgaaaagcc ctcgcctga agtaacccc 
      cttttacaa ggcgactga acacggggc cctcaaaac 
      caggaccat gggggccac gagcacgcc cctgattca 
      caaagatag cgacaaaag cgataaaat caccccgat 
      taatcagag cgaaattat ctaccataa atcaagctt 
      gaagctaac agggtcgtc gaatttcag cgactccaa 
      gaaaagcga ccgcagggt cactttttc ggaaacccc 
      ctttgtgag tgaaatgtg caaaaagcc caagtattt 
      acctttgcc taaacaaat aacgaatga aaattcaaa 
      cgtttccaa tgacgactc aaccgatga actggaatt 
      gtacaaaac agcccctca cacaattct agaatgcga 
      agaaaagga gaatttaga taacattgg cgtatttaa 
      taattgtga cgctaataa cgacgggac cttatggga 
      aagtgcttt aacaaaatt ggatttcag cggctgtaa 
      taacatcat tgagaagat tgacaacaa aacgaataa 
      ttttaacgt ttcgctgca aataaatgt gttcagccc 
      gccaaatag tccaaaatc gaaaagcaa cgacgctga 
      cttgtcttt gaataaatc cacaaacat ttggtcctc 
      tgtgacaag catggtcaa gtcggtcgt gagcaacat 
      ctcaatgac ggcgcagag tagtttttc aggcgtagg 
      tctcaaaag gccgcagcc tgataatga cgcactttg 
      cgacgcccc acatttgaa gagaatgaa actgaccga 
      cattaagtg ccgacggcc aatcaggac cataagtcc 
      cttgtatca aataagact cgcacgccc ccgttatgt 
      agacaaagc gactcaaac cgactaaat ttggatgtg 
      atttttacc aaactacat ttaactacc cttttttgt 
      atttaatgt aattacttt gaaataaat tatgtgata 
      atta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY