Status
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Gene information
General information
TC031476
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011553
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3854160..3858235
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031476
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06554

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head capsule mostly size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031476 RA
      MAPKTLKLI IISTSLKFR PMCKPPYTV IRPNNKHSL 
      NCRFAITDT AFRSLKNEN REQCILISG ESGSGKTEA 
      SKKILQYIA EITDHRGDV ERVKDKLLQ SNPVLEAFG 
      NAKTNRNDN SSRFGKYMD IQFNYEGNP SGGNILNYL 
      LEKSRVISQ VPGERNFHI FYQLLAGAD EQELQRLNL 
      KRDLDSYYY ISNGAKRNG GPVDDDKGF RDVKNALTV 
      IEFDQKEQD EIFAITAAI LHLGNIGFS EENGVSTII 
      KPELVETVS RLLGCSLED LKNALTNRT IETIRDVVT 
      TPLTRELAI YARDALAKA VYDRLFTWL VHRLNTSLK 
      PHDRRTYSV MGILDIYGF EIFEKNSFE QLCINFCNE 
      KLQQLFIEL TLKSEQEEY LKEGIEWQN VEYFDNKVI 
      CDLIEEKHK GLIAFMDEE CLRPGDSSD MTMLNKLDK 
      NLSYHKHFI SHKKADVKL QKIMGRDEF RLIHYAGAV 
      TYNVKGFLE KNNDLLFRD LRETMSKSS NNVIQSVFL 
      KEEQQSKKR PNTAITQFK NSLNNLMHI LMDKEPSYI 
      RCIKPNGDK RSGVFDEEL VRHQVTYLG LMENLRVRR 
      AGFAYRREY GLFLERYKC LSKQTWPKY IGNPKDGVQ 
      ILVCALGYE ADEYKMGKT KIFIRYPKT LFYTEDAFQ 
      AKKHDLAAL IQAAWKGLV QRRKYKKMK EASVIMQKY 
      VRMFIAKCR ARKRRRAAN VLRNFIKGF ITRNGPATE 
      ENKAFLELA KAQWLVRLS KNLPQNILR RTWPPCPHI 
      CIEASQHLE RLYGAHMSR VYRLKLSPE REEQLRLKV 
      LAEKLFKDK KKSYQASVS RWFKTDRVT EAGVAKQSY 
      AQVLNGERE QYVTTVMKY DRHGYKPRQ RVVLITTKN 
      FYVIEVKNS SAKQKHCLP LSKLNFVVT PNNDKMLLV 
      RIPEDLLKK DKGDLILEV PHLIEAVTY IINVTDNPG 
      LLTIIDKSP IQHSMKQGK RGTIDVIIG NSESIHKDK 
      SGHLIVASS
    CDS sequence
    • >TC031476 RA
      atggcccca aagacgtta aagcttatt ataataagc 
      acttctttg aagttccgc cccatgtgt aagccccct 
      tacacagtc attaggcct aataataaa cactcatta 
      aattgtaga tttgcgata acggacact gccttcagg 
      tcgttaaaa aacgaaaac agggaacaa tgcatttta 
      atttctggc gagtccggt tctgggaag actgaagca 
      tcgaaaaag atactccaa tacattgcc gaaatcacc 
      gaccatcga ggagacgtt gaaagggtc aaagacaag 
      cttcttcaa tcaaatccg gttttggag gctttcgga 
      aacgcaaaa acaaaccgc aatgacaat tctagcagg 
      tttggtaaa tacatggac attcagttt aattacgaa 
      gggaatcct tctggtgga aacatcctg aactatctg 
      ctagaaaag tcgcgtgtt atcagtcaa gtgcctggc 
      gaaagaaat tttcatatt ttttatcaa ctccttgcc 
      ggcgcggac gaacaagaa ttacaaaga cttaactta 
      aaacgggac ttagattcg tactattac atctccaat 
      ggagcgaag agaaacggg ggcccggtt gacgacgac 
      aaaggcttc cgtgacgtg aaaaacgca ctaacagta 
      attgagttt gatcagaaa gaacaagac gagattttt 
      gccattaca gcggcgatt ctgcacttg gggaacatc 
      ggattttcg gaggaaaac ggcgtttcc acaatcatt 
      aaaccagaa cttgtggaa actgtcagc aggctgctt 
      ggttgcagt ctcgaagat ttgaaaaat gctctcaca 
      aatcgtaca attgaaacg attagagac gtggtaaca 
      actccatta acccgagaa ttggccatt tatgcacgc 
      gatgctttg gcaaaagcg gtgtacgat cgcctcttc 
      acttggctt gttcacaga cttaacact tccctcaaa 
      ccgcacgac aggaggacc tacagcgtg atgggcatt 
      ttggatatt tatgggttc gaaattttc gaaaaaaac 
      agtttcgag cagctgtgt ataaatttt tgcaacgag 
      aagctgcaa caacttttc attgaactg actctcaag 
      tcggaacaa gaggagtat ttgaaggaa gggatcgag 
      tggcagaat gttgagtac tttgataac aaagttatc 
      tgcgaccta attgaagaa aaacataaa ggacttatc 
      gcctttatg gacgaagag tgcctccga ccgggggat 
      tcctccgac atgacaatg ttgaataaa ttagataag 
      aacctaagt taccacaaa cacttcatc agccataaa 
      aaggcagac gtgaagtta caaaaaatt atgggccgt 
      gatgaattt cgcctaatt cattacgct ggtgctgta 
      acgtacaat gttaaaggt tttctcgag aaaaacaac 
      gacttgctt ttccgcgac ttgagagaa actatgtcc 
      aaatcttcc aataatgtc atccaatca gttttcttg 
      aaagaggaa caacagagc aaaaagcga cccaacact 
      gccattacc caatttaaa aattcgctg aacaatctg 
      atgcacatt ttgatggac aaggaaccg tcgtacatc 
      aggtgcatc aaaccgaac ggtgataaa agatcaggc 
      gtctttgac gaggagttg gtacgtcac caagtgaca 
      tacttgggc cttatggaa aatttgcga gtacgccgc 
      gctggtttt gcctaccga cgtgaatac ggcctgttc 
      ctggagcgc tacaaatgt ttgtcgaaa caaacttgg 
      cccaaatac atcggaaac ccaaaagac ggcgtccaa 
      attctagtc tgtgccctt ggctacgaa gccgacgag 
      tacaaaatg ggcaaaaca aaaatcttc atccgctac 
      ccgaagacg ctcttctac accgaggac gccttccag 
      gccaaaaaa cacgacttg gccgccctt atccaagcc 
      gcctggaag ggccttgtc cagcggcga aagtacaaa 
      aaaatgaaa gaggcgtcc gtaatcatg caaaaatac 
      gtgcggatg ttcatcgct aaatgtcgc gctaggaag 
      cgcagaagg gccgcgaac gttttgcgc aacttcatc 
      aagggtttt atcacccga aatgggccc gcaactgag 
      gaaaacaag gcgtttttg gagctggct aaagcccag 
      tggttggtg cgcctgtcg aaaaacctg ccccagaat 
      atactcagg cggacgtgg cccccttgt ccccacatc 
      tgcatcgag gcgtcgcaa cacctggag cgcctctat 
      ggggcccac atgtcgcgg gtgtaccga ctgaagttg 
      agccctgag agggaggaa cagctgagg cttaaagtc 
      cttgccgag aagttgttc aaagacaag aaaaagagc 
      taccaagcg agtgtttcg cggtggttt aagacggat 
      cgggtgacg gaggctggg gtggccaaa cagagctat 
      gctcaagtc ttgaatggg gagagagaa cagtacgtt 
      accacagtc atgaaatac gaccggcat ggttataaa 
      ccgcgccaa cgcgtcgtt ttgataacg accaaaaat 
      ttctacgtt atcgaagtg aagaattct tcggctaaa 
      caaaagcac tgtttgcct ttgtccaaa ctgaacttt 
      gtcgtgacg ccaaacaac gataagatg ctgctggtg 
      aggattccg gaagatttg ttgaagaaa gataaaggc 
      gatttaatt ttggaagtt ccacattta atcgaggct 
      gttacttat atcataaat gtgactgat aatcccggc 
      ctgttgacc attattgat aagtcgccg attcaacat 
      tcgatgaag caagggaaa cggggaacg attgatgtg 
      attattggg aactcggaa agtatacat aaggataaa 
      agtggtcat ttaattgtg gcttcctcg taa
    mRNA sequence
    • >TC031476 RA
      cggctattt taaacaatt tgtatttga atattattt 
      attttagac gtacatcgg gcaagtgct aatttcggt 
      aaaccctta caagcagtt ggatattta tggccccaa 
      agacgttaa agcttatta taataagca cttctttga 
      agttccgcc ccatgtgta agccccctt acacagtca 
      ttaggccta ataataaac actcattaa attgtagat 
      ttgcgataa cggacactg ccttcaggt cgttaaaaa 
      acgaaaaca gggaacaat gcattttaa tttctggcg 
      agtccggtt ctgggaaga ctgaagcat cgaaaaaga 
      tactccaat acattgccg aaatcaccg accatcgag 
      gagacgttg aaagggtca aagacaagc ttcttcaat 
      caaatccgg ttttggagg ctttcggaa acgcaaaaa 
      caaaccgca atgacaatt ctagcaggt ttggtaaat 
      acatggaca ttcagttta attacgaag ggaatcctt 
      ctggtggaa acatcctga actatctgc tagaaaagt 
      cgcgtgtta tcagtcaag tgcctggcg aaagaaatt 
      ttcatattt tttatcaac tccttgccg gcgcggacg 
      aacaagaat tacaaagac ttaacttaa aacgggact 
      tagattcgt actattaca tctccaatg gagcgaaga 
      gaaacgggg gcccggttg acgacgaca aaggcttcc 
      gtgacgtga aaaacgcac taacagtaa ttgagtttg 
      atcagaaag aacaagacg agatttttg ccattacag 
      cggcgattc tgcacttgg ggaacatcg gattttcgg 
      aggaaaacg gcgtttcca caatcatta aaccagaac 
      ttgtggaaa ctgtcagca ggctgcttg gttgcagtc 
      tcgaagatt tgaaaaatg ctctcacaa atcgtacaa 
      ttgaaacga ttagagacg tggtaacaa ctccattaa 
      cccgagaat tggccattt atgcacgcg atgctttgg 
      caaaagcgg tgtacgatc gcctcttca cttggcttg 
      ttcacagac ttaacactt ccctcaaac cgcacgaca 
      ggaggacct acagcgtga tgggcattt tggatattt 
      atgggttcg aaattttcg aaaaaaaca gtttcgagc 
      agctgtgta taaattttt gcaacgaga agctgcaac 
      aacttttca ttgaactga ctctcaagt cggaacaag 
      aggagtatt tgaaggaag ggatcgagt ggcagaatg 
      ttgagtact ttgataaca aagttatct gcgacctaa 
      ttgaagaaa aacataaag gacttatcg cctttatgg 
      acgaagagt gcctccgac cgggggatt cctccgaca 
      tgacaatgt tgaataaat tagataaga acctaagtt 
      accacaaac acttcatca gccataaaa aggcagacg 
      tgaagttac aaaaaatta tgggccgtg atgaatttc 
      gcctaattc attacgctg gtgctgtaa cgtacaatg 
      ttaaaggtt ttctcgaga aaaacaacg acttgcttt 
      tccgcgact tgagagaaa ctatgtcca aatcttcca 
      ataatgtca tccaatcag ttttcttga aagaggaac 
      aacagagca aaaagcgac ccaacactg ccattaccc 
      aatttaaaa attcgctga acaatctga tgcacattt 
      tgatggaca aggaaccgt cgtacatca ggtgcatca 
      aaccgaacg gtgataaaa gatcaggcg tctttgacg 
      aggagttgg tacgtcacc aagtgacat acttgggcc 
      ttatggaaa atttgcgag tacgccgcg ctggttttg 
      cctaccgac gtgaatacg gcctgttcc tggagcgct 
      acaaatgtt tgtcgaaac aaacttggc ccaaataca 
      tcggaaacc caaaagacg gcgtccaaa ttctagtct 
      gtgcccttg gctacgaag ccgacgagt acaaaatgg 
      gcaaaacaa aaatcttca tccgctacc cgaagacgc 
      tcttctaca ccgaggacg ccttccagg ccaaaaaac 
      acgacttgg ccgccctta tccaagccg cctggaagg 
      gccttgtcc agcggcgaa agtacaaaa aaatgaaag 
      aggcgtccg taatcatgc aaaaatacg tgcggatgt 
      tcatcgcta aatgtcgcg ctaggaagc gcagaaggg 
      ccgcgaacg ttttgcgca acttcatca agggtttta 
      tcacccgaa atgggcccg caactgagg aaaacaagg 
      cgtttttgg agctggcta aagcccagt ggttggtgc 
      gcctgtcga aaaacctgc cccagaata tactcaggc 
      ggacgtggc ccccttgtc cccacatct gcatcgagg 
      cgtcgcaac acctggagc gcctctatg gggcccaca 
      tgtcgcggg tgtaccgac tgaagttga gccctgaga 
      gggaggaac agctgaggc ttaaagtcc ttgccgaga 
      agttgttca aagacaaga aaaagagct accaagcga 
      gtgtttcgc ggtggttta agacggatc gggtgacgg 
      aggctgggg tggccaaac agagctatg ctcaagtct 
      tgaatgggg agagagaac agtacgtta ccacagtca 
      tgaaatacg accggcatg gttataaac cgcgccaac 
      gcgtcgttt tgataacga ccaaaaatt tctacgtta 
      tcgaagtga agaattctt cggctaaac aaaagcact 
      gtttgcctt tgtccaaac tgaactttg tcgtgacgc 
      caaacaacg ataagatgc tgctggtga ggattccgg 
      aagatttgt tgaagaaag ataaaggcg atttaattt 
      tggaagttc cacatttaa tcgaggctg ttacttata 
      tcataaatg tgactgata atcccggcc tgttgacca 
      ttattgata agtcgccga ttcaacatt cgatgaagc 
      aagggaaac ggggaacga ttgatgtga ttattggga 
      actcggaaa gtatacata aggataaaa gtggtcatt 
      taattgtgg cttcctcgt aattgtccg accagtgcc 
      ttaattggt gaaacaaaa aataatcat ataattgtg 
      taaaaagtg ccattacga caaatactt gtgtactta 
      ttaatttta cgttgcttt tcattatac atgtttaca 
      tatatttat tgtgggcct taatagttt attaaatta 
      aatactaaa ctaaaaata tatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY